• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.17-17
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• 2010

Ethylene, known as a stress hormone regulate wide developmental processes including germination, root hair initiation, root and shoot primordial formation and elongation, leaf and flower senescence and abscission, fruit ripening. The acceleration of ethylene biosynthesis in plant associated with environmental and biological stresses. 1-Aminocycloprophane-1-carboxlyate deaminase(ACCD) is an enzyme that cleaves ACC into and ammonia, a precursor of the plant hormone ethylene. Plant growth-promoting rhizobacteria (PGPR) having ACCD can decrease endogenous ACC level of tissue, resulting in reduced production of ethylene in plants. ACC deaminse was a key enzyme for protect stressed plants from injurious effects of ethylene. ACCD gene was encoded from Pseudomonas flourescens, PGPR and was cloned in Escherichia coli. We expressed the recombinant ACCD(rACCD) containing 357 amino acids with molecular weight 39 kDa that revealed by SDS-PAGE and western blot. The rACCD was purified by Ni-NTA purification system. The active form of rACCD having enzyme activity converted ACC to a-ketobutyrate. The optimal pH for ACC deaminase activity was pH 8.5, but no activity below pH 7.0 and a less severe tapering activity at base condition resulting in loss of activity at over pH 11. The optimal temperature of the enzyme was $30^{\circ}$ and a slightly less severe tapering activity at 15 - 30$^{\circ}$, but no activity over $35^{\circ}$. P. flourescens ACC deaminase has a highly conserved residue that plays in allowing substrate accessibility to the active sites. The enzymatic properties of this rACCD will provide an important reference for analysis of newly isolated ACCD and identification of newly isolated PGPR containing ACCD.

• Journal of Ginseng Research
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• v.40 no.2
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• pp.97-104
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• 2016

Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.

• Journal of Internet Computing and Services
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• v.14 no.4
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• pp.25-33
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• 2013

The procedure of the arbitration which is the tag collision is essential because the multiple tags response simultaneously in the same frequency to the request of the Reader. This procedure is known as Anti-collision and it is a key technology in the RFID system. In this paper, we propose the 4-Bit Pattern Slot Allocation(4-BPSA) algorithm for the high-speed identification of the multiple tags. The proposed algorithm is based on the tree algorithm using the time slot and identify the tag quickly and efficiently through accurate prediction using the a slot as a 4-bit pattern according to the slot allocation scheme. Through mathematical performance analysis, We proved that the 4-BPSA is an O(n) algorithm by analyzing the worst-case time complexity and the performance of the 4-BPSA is improved compared to existing algorithms. In addition, we verified that the 4-BPSA is performed the average 0.7 times the query per the Tag through MATLAB simulation experiments with performance evaluation of the algorithm and the 4-BPSA ensure stable performance regardless of the number of the tags.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1992.10a
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• pp.25-30
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• 1992

최근 전자계산기를 이용한 진동해석 방법이 눈부시게 발달하여, 일반 구조물 이나 기계 구조물 등의 동특성을 설계 단계에서 정도 높게 예측하는 것이 가능하게 되었다. 그러나 종래의 구조해석은 주어진 시스템의 동특성을 위한 것으로 얻어진 동특성으로부터 질량, 관성제원 및 스프링상수값 등의 설계상 수값을 규명하는 연구는 미미한 실정이다. 이것에 대한 해결방법으로 크게 해석적인 방법과 실험적인 방법으로의 접근이 있어 왔다. 해석적인 방법으로 유한요소해석에서 얻은 모드좌표를 물리좌표로 변환하는 방법으로 Guyan의 정축소와 같은 절점축소를 행하는 방법이 고찰되었다. 실험적인 방법으로 가 진실험에서 얻은 전달함수나 모드파라미터로부터 [M], [K] 행렬을 결정하는 연구가 있었지만 어떤것도 질량, 스프링상수 등의 설계상수를 완전히 규명하 지는 못하였다. 또한, 설계 단계에서 필요한 질량, 관성제원 또는 스프링상수 등의 최적한 값이나, 원하는 시스템특성을 얻을 수 있는 설계상수의 적정한 폭을 구하는 연구는 설계자의 경험과 반복된 시행착오에 의존하는 실정이다. 감도해석은 이러한 문제점을 개선하는 수단으로 설계변수에 대한 동특성의 변화율을 구하는 것이다. 감도해석을 수행하는 것은 어느 설계변수를 수정하 는 것이 주어진 동특성에 부합되는 지를 알려주고, 어느 것을 수정하는 것이 원하는 방향의 동특성변화에 가장 효과적인지를 알려주는 것이다. 따라서 감 도해석을 이용하여 설계의 최적화 프로그램을 만들수 있고, 이것은 설계자가 요구하는 동특성을 목적함수로 하여 주어진 구조물을 최적화하는 설계상수 값을 얻을 수 있게 한다. 본 논문에서는 강체모델의 동특성으로부터 모델의 설계 상수를 규명하고, 동특성의 개선을 위하여 설계변수의 변경량을 물리좌 표계에서 얻는것을 목적으로 한다. 강체 마운트계의 관성제원 및 마운트강성 의 규명을 위하여 임으로 주어진 설계상수를 모델데이타로 하여 관성제원과 스프링 강성을 구하였다. 관성제원의 규명은 주어진 모델의 관성값을 모르는 것으로 하여 임의의 초기 관성값으로 감도해석에 의해 주어진 계의 관성값 을 물리 좌표계에서 규명하였다. 마운트 강성의 규명도 관성제원의 규명과 같은 방법으로 임의의 강성값으로 감도해석을 하여 강성값을 규명하였다. 또 한 감도해석에 의한 동특성 변경은 특정한 고유진동 수의 변경이 필요할 때, 고유진동수의 이동을 위한 관성제원의 변경 및 마운트 강성변경값을 예측할 수 있다. 본 연구수행의 기본적인 흐름도는 Fig.1.1과 같다. 위와 같은 작업 으로 엔진 마운트와 같은 강체 모델의 시스템 규명을 행하는 경우에 유한요 소해석 및 가진 실험으로 얻은 고유진동수의 정보 또는 원하는 고유진동수 의 특성을 기본으로 실제 설계에서 사용이 가능하도록 물리 좌표계에서 관 성 제원 및 스프링상수를 구할 수 있을 것이다.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.4
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• pp.267-272
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• 2008

Mid-infrared (MIR) spectroscopy, particularly Fourier transform infrared spectroscopy (FTIR), has emerged as an important analytical tool in quantification as well as identification of multi-atomic inorganic ions such as nitrate. In the present study, the possibility of quantifying soil nitrate via diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) without change of a sample phase or with least treated samples was examined. Four types of soils were spectrally characterized in terms of unique bands of soil contents and interferences with nitrate bands in the range of $2000-1000cm^{-1}$. In order to reduce the effects of soil composition on calibration model for nitrate, spectra transformed to the 1st order derivatives were used in the partial least squared regression (PLSR) model and the classification procedure associated with input soil types was involved in calibration system. PLSR calibration models for each soil type provided better performance results ($R^2$>0.95, RPD>6.0) than the model considering just one type of soil as a standard.

• Child Health Nursing Research
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• v.7 no.4
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• pp.421-433
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• 2001

The purpose of this phenomenological study was to understand the lived experience of mothers of children with muscular dystrophy. The participants were 11 mothers recruited by snowball sampling, who agreed with the objectives of the research and could verbally communicate with the researcher and who lived with their sons who had muscular dystrophy. Data collection was done through in-depth interviews with participants in their own homes. Each interview took 50 to 120 minutes. All of the interviews were audiotaped and then transcribed. Data were analyzed using Colaizzi's (1978) method of phenomenology. From the transcripts 109 significant sentences and phrases were selected and 13 clusters of themes were categorized from 67 significant statements. These results were integrated into the essential structure of the lived experience of mothers of children with muscular dystrophy. 1. Anxious and relying on chance due to indefinite diagnosis. 2. Only able to cry with shock because of son's fatal disease. 3. Seeing the future as dismal and feeling resentment that this disease in transmitted through the mother. 4. Feeling caught between a husband who is distancing himself from his family and the family which is disintegrating. 5. Feeling like a sinner for transmission of genetic disease(Feeling guilt for conceiving a son with a genetically transmitted fatal disease). 6. Empathizing with other suffers of muscular dystropy and their parents in their grief and helping to dissipate their heavy feelings. 7. Deciding to sacrifice self as a way of taking responsibility for giving birth to a handi-capped son. 8. Losing hope (feeling despair) as son's condition deteriorates in spite of all attempts to help him. 9. Wishing to die with son who becomes progressively immobile. 10. Accumulating Han*(한, 恨), because of rising Hwa(화, anger), and becoming sick both mentally and physically. 11. Seeing events as destiny and finding self-control through faith. 12. Finally, giving up sacrificing self for son and becoming concerned(involved) with other children in the family. 13. Feeling fear at son's impending death. This is the first research on the experience of Korean mothers of children with muscular dystrophy. In applying the phenomenology research method, this study not only helps health professionals understand the experience of these mothers in the Korean patriarchal social system, but the researcher, as a nurse, can share their agony and grief through identification of their inner world through in-depth personal interviews. The results obtained in this study will not only help in the development of family nursing practice for families with genetically transmitted diseases but also prepare basic data for family nursing practice in the Korean sociocultural context.

• Progress in Medical Physics
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• v.17 no.4
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• pp.192-200
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• 2006

We evaluated the positional accuracy of the delivered beams to the target in a phantom by simulating the whole process of the radiation treatments Including CT scanning, planning and beam exposures with MLCs. For this purpose, a phantom was made to calibrate the alignment between the CT and the attached laser system. A new, convenient method was also devised to align the setup lasers in the treatment room. Film was used for the Identification of the delivered beam and analyzed with a homemade computer program. The positional differences between the target and the beam centers varied with the couch rotations. The accelerator we used showed a maximum discrepancy of 2.0 mm at the table angle of $295^{\circ}$. The same measurements based on the new isocenter from the Winston-Lutz test resulted in the maximum of 1.35 mm for all rotation angles. The evaluation of the differences between the target and the beam centers is useful for the treatment planning.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1917-1923
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• 2016

Background: Lynch Syndrome (LS) is a familial cancer condition caused by germline mutations in DNA mismatch repair genes. Individuals with LS have a greatly increased risk of developing colorectal cancer (CRC) and it is therefore important to identify mutation carriers so they can undergo regular surveillance. Tumor DNA from LS patients characteristically shows microsatellite instability (MSI). Our aim here was to screen young CRC patients for MSI as a first step in the identification of unrecognized cases of LS in the Saudi population. Materials and Methods: Archival tumor tissue was obtained from 284 CRC patients treated at 4 institutes in Dammam and Riyadh between 2006 and 2015 and aged less than 60 years at diagnosis. MSI screening was performed using the BAT-26 microsatellite marker and positive cases confirmed using the pentaplex MSI analysis system. Positive cases were screened for BRAF mutations to exclude sporadic CRC and were evaluated for loss of expression of 4 DNA mismatch repair proteins using immunohistochemistry. Results: MSI was found in 33/284 (11.6%) cases, of which only one showed a BRAF mutation. Saudi MSI cases showed similar instability in the BAT-26 and BAT-25 markers to Australian MSI cases, but significantly lower frequencies of instability in 3 other microsatellite markers. Conclusions: MSI screening of young Saudi CRC patients reveals that approximately 1 in 9 are candidates for LS. Patients with MSI are strongly recommended to undergo genetic counselling and germline mutation testing for LS. Other affected family members can then be identified and offered regular surveillance for early detection of LS-associated cancers.

• Atmosphere
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• v.13 no.4
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• pp.57-70
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• 2003

The National Center for Intensive Observation of Severe Weathers (NCIO) as a part of METRI's principal project "Korea Enhanced Observing Period; KEOP" was established at Haenam Weather Observatory in order to effectively monitor and observe heavy rainfall in summer, which is essential for the identification of the structure and evolution mechanism of mesoscale severe weather system. The intensive field-based experiments in 2002 within southwestern Korea toward various meteorological phenomena ranging from heavy rainfall to snowfall were conducted in collaboration with KMA(Korea Meteorological Administration) and universities. In this study, preliminary analysis results using intensive observation data obtained from these experiments are presented together with the introduction of NCIO and its operational structure.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.4
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• pp.480-488
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• 2018

Objective: Average daily gain (ADG) is an important target trait of pig breeding programs. We aimed to identify single nucleotide polymorphisms (SNPs) and genomic regions that are associated with ADG in the Duroc pig population. Methods: We performed a genome-wide association study involving 390 Duroc boars and by using the PorcineSNP60K Beadchip and two linear models. Results: After quality control, we detected 3,5971 SNPs, which included seven SNPs that are significantly associated with the ADG of pigs. We identified six quantitative trait loci (QTL) regions for ADG. These QTLs included four previously reported QTLs on Sus scrofa chromosome (SSC) 1, SSC5, SSC9, and SSC13, as well as two novel QTLs on SSC6 and SSC16. In addition, we selected six candidate genes (general transcription factor 3C polypeptide 5, high mobility group AT-hook 2, nicotinamide phosphoribosyltransferase, oligodendrocyte transcription factor 1, pleckstrin homology and RhoGEF domain containing G4B, and ENSSSCG00000031548) associated with ADG on the basis of their physiological roles and positional information. These candidate genes are involved in skeletal muscle cell differentiation, diet-induced obesity, and nervous system development. Conclusion: This study contributes to the identification of the casual mutation that underlies QTLs associated with ADG and to future pig breeding programs based on marker-assisted selection. Further studies are needed to elucidate the role of the identified candidate genes in the physiological processes involved in ADG regulation.