• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.186-195
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• 2015

Anthranilate synthase (AS) is a key enzyme in the biosynthesis of tryptophan (Trp), which is the precursor of bioactive metabolites like indole-3-acetic acid and other indole alkaloids. Alpha anthranilate synthase 2 (OsASA2) plays a critical role in the feedback inhibition of tryptophan biosynthesis. In this study, two vectors with single (F124V) and double (S126F/L530D) point mutations of the OsASA2 gene for feedback-insensitive ${\alpha}$ subunit of rice anthranilate synthase were constructed and transformed into wildtype Dongjinbyeo by Agrobacterium-mediated transformation. Transgenic single and double mutant lines were selected as a single copy using TaqMan PCR utilized nos gene probe. To select intergenic lines, the flanking sequence of RB or LB was digested with a BfaI enzyme. Four intergenic lines were selected using a flanking sequence tagged (FST) analysis. Expression in rice (Oryza sativa L.) of the transgenes resulted in the accumulation of tryptophan (Trp), indole-3-acetonitrile (IAN), and indole-3-acetic acid (IAA) in leaves and tryptophan content as a free amino acid in seeds also increased up to 30 times relative to the wildtype. Two homozygous event lines, S-TG1 and D-TG1, were selected for characterization of agronomic traits and metabolite profiling of seeds. Differentially expressed genes (DEGs), related to ion transfer and nutrient supply, were upregulated and DEGs related to co-enzymes that work as functional genes were down regulated. These results suggest that two homozygous event lines may prove effective for the breeding of crops with an increased level of free tryptophan content.

• Journal of Korean Society of Steel Construction
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• v.15 no.5 s.66
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• pp.499-507
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• 2003

As the load is increased on the steel-concrete composite structure, its interface begins to show nonlinear behavior due to the reduction of interaction, micro-crack, slip and separation, and it causes slip-softening, Therefore, it is essential to consider the partial-interaction analysis technique. Until now, however, full-interaction or, in some instances, the linear-elastic model, which are insufficient to simulate accurate behavior, are assumed in the analysis of composite structure since the analysis method and nonlinear model for interface are very difficult and complicated. Therefore, the design of composite structure is followed by the experimental method which is inefficient-because a number of tests have to be carried out according to the design environments. In this study, we carried out the nonlinear analysis according to various interface nonlinear models by interaction magnitude, and analyzed more accurate structural behavior and performance by maximum tangential traction and slip-softening at the interface. As a result of this study. we were able to prove that the nonlinear model of interface more exactly represents behavior after yielding, such as ultimate load: that initial tangential stiffness of interface has a significant effect on the yielding load of structural members or part: and that the maximum tangential traction and slip-softening mainly effects structural yielding and ultimate load. Therefore, the structural performance of composite structure is highly dependent on the steel-concrete interface or interaction, which may result in initial tangential stiffness, maximum tangential traction and slip-softening in nonlinear model.

• Korean Journal of Environmental Agriculture
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• v.20 no.3
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• pp.162-168
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• 2001

This study was conducted to evaluate the degradation, adsorption and desorption and leaching of acetamiprid in soils. The half-life of acetamiprid in field condition was $1.7{\sim}3.3$ days in Bokhyun soil and, in case of laboratory condition, 15.5 days. Adsorption of acetamiprid was equilibrated in 12 hours incubation. In adsorption experiment using modified soils, such as oxidized soil, oxidized soil added humic acid, fulvic acid, kaolinite or montmorillinite, adsorption rate of acetamiprid was the highest in the oxidized soil added fulvic acid. The desorption rate was the lowest in the oxidized soil added fulvic acid. The adsorption and desorption results should be suggested that acetamiprid could be strongly adsorbed with soil humic materials, especially fulvic acid. When the mobility of acetamiprid in soil was calculated according to GUS (Groundwater Ubiquity Score) equation, it was prove to non-leacher, and it was confirmed in the leaching experiment with soil column. Most of acetamiprid was remained in the upper 30 cm of the soil column after eluting with water and it was not even detected in leachate.

• Journal of the Korea Society of Computer and Information
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• v.16 no.8
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• pp.29-38
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• 2011

Unlike in traditional row-oriented database systems, a column-oriented database system stores data in column-oriented and not row-oriented order. Recently, research results revealed the effectiveness of column-oriented databases for applications such as data warehouse and decision support systems that access large volumes of data in a read only manner. In this paper, we investigate the join strategies for column-oriented databases and prove the effectiveness of column-oriented databases in data warehouse systems. For unbiased comparison, the two database systems are analyzed using the star schema benchmark and the performance analysis of a star schema join query is carried out. We experimented with well-known join algorithms and considered early materialization and late materialization join strategies for column-oriented databases. The performance results confirm that star schema join queries perform better in terms of disk I/O cost in column-oriented databases than in row-oriented databases. In addition, the late materialization strategy showed more performance gain than the early materialization strategy in column-oriented databases.

• Journal of Oral Medicine and Pain
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• v.32 no.2
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• pp.151-156
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• 2007

Antimicrobial action of phytoncide in the mouth decrease odor-producing microorganisms. Also phytoncide has malodor effect by reaction with volatile sulfur compounds. Phytoncide has excellent malodor effect in microbiologically and chemically. This study prove the malodor effect of phytoncide by use ferrous sulfate. So I try to make new treatment method for halitosis. I get the results as follows. 1. The difference of mean value of absorbancy was 0.849 between the mean absorbancy of deposition by add phytoncide to saliva and the saliva only. 2. The difference of mean value of absorbancy was 0.701 between the mean absorbancy of deposition by add phytoncide to distilled water and the distilled water only. 3. The difference of mean value(0.849) in saliva by existence of phytoncide was larger than in double distilled water(0.701) by existence of phytoncide. Therefore, phytoncide make more deposition in saliva than double distilled water by reaction with sulfur compounds. As the results, phytoncide reaction with sulfur compounds in saliva. It take malodor action in liquid state effectively. It is thought, only the toothpaste it knows from in the limit which does not have a side effect by the human body it adds in the oral cavity of the mouth rinse and with the fact that it will be able to use positively in clinic.

• Journal of Biomedical Engineering Research
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• v.18 no.3
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• pp.291-299
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• 1997

As a microimaging device detecting gamma rays emitted from small lesions or tumors during operation, the intraoperative surgical probe has been proposed and is now under development. We have designed a multipurpose portable gamma prove system and evaluated the performance both for the absolute counting purpose of residual radioactivities and for the localizing capability of gamma events using the NaI(Tl) crystal and two types of photomultiplier tubes(PMTs). Counting efficiencies in the range of routine clinical use of radiation dose were measured using the assembly of single channel PMTs and 0.5 inch thick NaI(Tl) crystal of 1 inch diameter. The positioning of gamma events for imaging purpose requires the multiple channel PMTs with appropriate positioning electronics. We have designed a simple and reliable positioning circuit based on the concept of modified Anger. In preliminary experiments using the multiple channel PMT of 3 inch diameter and the dim lighth source, we were able to trace and localize the correct position with reduced positioning error by the use of two multiplier/divider chipset and simplified peripherals. The energy resolutions for the counting gamma probe measured as full width at half maximum(FWHM) for Cs-137, F-18, Tc-99m were 12%, 13%, and 36%, respectively. The spatial resolution for the imaging gamma probe measured as FWHM for green LED was 2.9 mm. The results indicate that the currently developing probe is very promising and could be very useful for many applications in nuclear medicine. Future studies will include developing collimators, improving interface hardwares, and evaluating the system with clinical data.

• The Korean Journal of Air & Space Law and Policy
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• v.21 no.2
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• pp.55-69
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• 2006

Electronic ticketing ("e-ticketing") through the internet will be the most critical commercial tool in the international airline industry. Actually the International Air Transport Association(IATA) has announced that it's goal is to accomplish 100 percent implementation of e-ticketing worldwide by 2007. Domestically e-ticketing will be put into operation in a full scale from the next year(2007). Thus, we count on, sooner or later, e-ticketing will be accomplished globally. As a legal aspect, this e-ticketing raises many legal issues which are no longer subject to a static set of legal rules driven by judicial precedent because e-ticketing is carried out on the "cyber space" which is a new and developing law area. In this treatise, I studied these legal issues, specially on the time and place(jurisdiction) of the contract which are the most important issues based on the New Montreal Convention signed at Montreal on May 28, 1999, which would seem prove that the airline industry is receptive and capable of change. Upon this opportunity, I hope the more comprehensive legal study on the e-ticketing will be introduced in the near future.

• Cartoon and Animation Studies
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• s.39
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• pp.111-132
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• 2015

Although Rotoscoping technique has been used for a long time to mimic the natural and smooth motion since the early 20th century, its artistic value was devalued as tricks because it traces the already recorded images. But the fact that the rotoscoping images can cross the boundaries between animation and live action in an infinite integral freedom in the digital era became rather expansive new aesthetic possibilities of representation of the reality. In addition, Jonas Odell's animations such as (2010), (2008), (2006) are good example to prove that the rotoscoping images also can serve as means to enhance its narrative. This study is to analyze how rotoscoping images act as a unique role in relation with the narrative based on the said person's real stories and realistic images. I argue that his animated films constantly contain these three characteristics -Images to mediate Auditory sensitivity as a record of inner metaphysical reality, anonymous images to represent a specific existential character, and images that act as physical representation that holds the physical space/time and related memory. This reveals that rotoscoping images in this digital era went beyond reproduction for natural movements or special type of style. It rather suggests new layers of experience, and acquires new value in animation. I hope that this study could serve as a foundation to rediscover and re-position the value of rotoscoping images as well as good opportunity to introduce very talented swedish animation artist who already received global attention with his unique philosophical and aesthetic style.

• Journal of the Korean Wood Science and Technology
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• v.32 no.2
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• pp.73-81
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• 2004

To develope of the inexpensive anti-stain chemicals, it was conducted to investigate the inhibitory effectiveness of mycelial growth and anti-mold effectiveness against test fungi causing surface mold of wood, iron corrosivity and hygroscopicity of six fluoride-based chemicals made from industrial by- product.Among the six chemicals. RNF-3 and RNF-4 were the most effective with respect to a mycelial growth control and anti-mold effectiveness. For the wood treatment with these chemicals, the optimum concentration was about 2% and there was no difference in the effectiveness of chemicals between wood species. The wood treated with synthesized chemicals showed a relatively high iron corrosion rate for corrosive F component whereas, in the treatment with RNF-3 and RNF-4, there was no difference from the untreated wood when the concentration was less than 2%. The hygroscopic property of wood was not effected by treatment of these chemicals. To prove the feasibility for practical using of RNF-3 and RNF-4 chemicals, it is necessary to test of anti-mold effectiveness in the sawmill by field test.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.75-83
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• 2021

This study will analyze the meaning of the signifiant, which occupies an absolute position in Lacanian psychoanalysis, and will prove the slip of meaning and signification that are accompanied together at the same time when the signifiant was utter through the subject. By directly citing the part where Lacan explained signification in his seminars and Écrits, I would like to examine how signifiant is carried out in everyday conversation. In addition, the dialogue that takes place in our discourse has the purposefulness and groundless purposelessness of the signifiant. Understanding this purpose is the core part that Lacanian psychoanalysis aims to pursue, and it discovers the cracks of the hidden meaning in the relationship between the signifiant and the signifiant connected to the next, presenting that the signifiant which arouses unrelenting fantasy of the subject is the practical ruler of body and mind. This thesis is aimed to present the above points mentioned above, and as an alternative to overcome the limitations of the signifiant, the ruler in discourse, this study would like to suggest the autonomy as a subject resisting against "Where it was, I must come into being," pursued by Lacan psychoanalysis.