• Journal of Yeungnam Medical Science
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• v.5 no.2
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• pp.37-45
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• 1988

Phosphoinositide-specific phospholipase C(PI-PLC) is a second messenger of signal transducer on cell membrane. In the previous study, PLC of bovine brain has been purified three isozymes. In this paper, uterus and seminal vesicle have been purified. Two peaks of PI-PLC activity were resolved when bovine uterus and seminal vesicle proteins were chromatographed on a DEAE and phenyl TSK 5PW HPLC column. Each two peak was compared with PI-PLC I, IT and ill from bovine brain and we got the retension time on HPLC. The peak fractions with PLC activity were tested homogeneity with brain PLC monoclonal antibodies(Mab). Mab-labeled affigels were bounded in the range of 73.8%~97.5% with PLC I, IT and III. Homogeneity of fractions were revealed that DEAE F-1 and phenyl F-1-I were highest level of PLC III in uterus and seminal vesicle and DEAE F-2 and phenyl F-2-I were mixed PLC I and II.

• Molecules and Cells
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• v.19 no.3
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• pp.375-381
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• 2005

Phospholipase C-${\beta}$ (PLC-${\beta}$) hydrolyses phosphatidylinositol 4,5-bisphosphate and generates inositol 1,4,5-trisphosphate in response to activation of various G protein-coupled receptors (GPCRs). Using glial cells from knock-out mice lacking either PLC-${\beta}1$ [PLC-${\beta}1$ (-/-)] or PLC-${\beta}3$ [PLC-${\beta}3$ (-/-)], we examined which isotype of PLC-${\beta}$ participated in the cellular signaling events triggered by thrombin. Generation of inositol phosphates (IPs) was enhanced by thrombin in PLC-${\beta}1$ (-/-) cells, but was negligible in PLC-${\beta}3$ (-/-) cells. Expression of PLC-${\beta}3$ in PLC-${\beta}3$ (-/-) cells resulted in an increase in pertussis toxin (PTx)-sensitive IPs in response to thrombin as well as to PAR1-specific peptide, while expression of PLC-${\beta}1$ in PLC-${\beta}1$ (-/-) cells did not have any effect on IP generation. The thrombin-induced $[Ca^{2+}]_i$ increase was delayed and attenuated in PLC-${\beta}3$ (-/-) cells, but normal in PLC-${\beta}1$ (-/-) cells. Pertussis toxin evoked a delayed $[Ca^{2+}]_i$ increase in PLC-${\beta}3$ (-/-) cells as well as in PLC-${\beta}1$ (-/-) cells. These results suggest that activation of PLC-${\beta}3$ by pertussis toxin-sensitive G proteins is responsible for the transient $[Ca^{2+}]_i$ increase in response to thrombin, whereas the delayed $[Ca^{2+}]_i$ increase may be due to activation of some other PLC, such as PLC-${\beta}4$, acting via PTx-insensitive G proteins.

• Journal of Institute of Control, Robotics and Systems
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• v.12 no.1
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• pp.1-8
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• 2006

The home network system guarantees families a safe, economical, socially integrated and healthy life by using information appliances. And it provides a family with domestic safety, control of instruments, controllable energy and health monitoring by connecting to home appliances. This study designs the broadband PLC home controller using broadband PLC(Power Line Communication) technology which can save much cost at a network infrastructure by using the existing power line at home. The broadband PLC home controller consists of the broadband PLC module, the embedded main controller module and I/O module. The broadband PLC home controller can control various domestic appliances such as an auto door-lock, a boiler, an oven, etc., because it has various I/O specifications. In this study, selected home network middleware for the broadband PLC home controller is Jini surrogate using Jini technology designed by means of access to easily a home network system without a limitation of the devices. And a client application program is supported java servlet program to manage and monitor the broadband PLC home controller via web browser of a PC or a PDA, etc. Finally, for an application, we implemented and tested a home security system using one broadband PLC home controller.

• Journal of Institute of Control, Robotics and Systems
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• v.11 no.4
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• pp.297-303
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• 2005

In this paper, I developed an intelligent multi agents based softPLC(IMPLC). In IMPLC, the standard IEC 1131-3 PLC languages(LD, SFC, FBD, ST) programmed by a user are converted to IL, which is one of intermediate codes, in order to make them interactions. And then the IL is converted to the standard C code regarding some extension and transplanting, which can be used in a commercial editor such as visual C++. In IMPLC, the logical errors and syntax errors occurred by users are detected, so that the optimal PC control based softPLC can be possible. IMPLC provide easy programming platform to such beginner as well as professionals. The study of code conversion is firstly tried in the world as well as KOREA. I applied IMPLC to 3 steps conveyer belt system. The simulation results say that the debugging steps by IMPLC using multi agents are decreased than the conventional softPLC's.

• Journal of the Korea Society for Simulation
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• v.25 no.4
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• pp.21-30
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• 2016

Recently manufacturing companies have used PLC control programs popularly for their automated production systems. Since the life cycle of production process is not so long, the change of the production lines occur frequently. Most of changes happen with modification of the position information and control process of the equipment. PLC control program is also modified based on the fundamental process. Therefore, to verify new PLC program by configuring virtual space according to real environment is needed. In this paper we show a logical modeling method, based on Timed-FSA useful for sequence control and dead-lock prevention. There is a problem wasting user's labor and time when defining a variety of states in a device. To overcome this problem, we propose an extended I-O model based on existing methods by adding a token concept of Petri Nets. Also we will show the usability of the extended I-O modeling through user study.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.19 no.37
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• pp.171-178
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• 1996

The programmable logic controller(PLC) programming has been manually carried out, which might cause the productivity decrease by omission and repetition of instruction. Recently, the knowledge-based automatic programming system is introduced. In the system, input/output(I/O) addresses are input by user. In this study, we developed the prototype system for automatic I/O addressing using knowledge-based technology, With the developed system, the PLC addressing task can he consistently and effectively performed.

• Journal of the Korean Society of Marine Environment & Safety
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• v.20 no.3
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• pp.318-322
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• 2014

A kind of field bus called Actuator and Sensor interface bus(AS-i) was designed in this paper. The configuration of AS-i network system used Application Specific Integrated Circuit(ASIC) SAP5S chip and PLC S7-200 station, which included CPU 224 and AS-i master module CP 243-2. We also created an example program for PLC S7-200 to control AS-i network. The fire and smoke detection system was made with AS-i network system that was designed. This system had got more advantages than other system such as number of stations, easy installation, wide working area, etc. And designed system can be used as a partner network for higher level field bus networks.

• Molecules and Cells
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• v.22 no.1
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• pp.65-69
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• 2006

In murine gastrointestinal myocytes muscarinic stimulation activates nonselective cation channels via a G-protein and $Ca^{2+}$-dependent pathway. We recorded inward cationic currents following application of carbachol ($I_{CCh}$) to murine gastric myocytes held at -60 mV, using the whole-cell patch-clamp method. The properties of the inward cationic currents were similar to those of the nonselective cation channels activated by muscarinic stimulation in other gastrointestinal smooth muscle cells. CCh-induced $I_{CCh}$ and spontaneous decay of $I_{CCh}$ (desensitization of $I_{CCh}$) occurred. Unlike the situation in guinea pig gastric myocytes, desensitization was not affected by varying $[EGTA]_i$. Pretreatment with the PLC inhibitor (U73122) blocked the activation of $I_{CCh}$, and desensitization of $I_{CCh}$ was attenuated in PLC ${\beta}_1$ knock-out mice. These results suggest that the desensitization of $I_{CCh}$ in murine gastric myocytes is not due to a pathway dependent on intracellular $Ca^{2+}$ but to the PLC ${\beta}_1$ pathway.

• 제어로봇시스템학회:학술대회논문집
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• 1999.10a
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• pp.61-64
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• 1999

In this paper, a technique to expand the Input and Output (I/O) of the programmable logic controller (PLC) using remote I/O module is presented. The controller and the remote I/O module should have the same protocol and are interfaced through RS 485. Each remote I/O module consists of 16 digital input and 16 digital output, and the maximum of 32 remote I/O module can be linked to one controller. The remote I/O is programmed for interrupt request to controller independently. Therefore, there is no affect to the scan time of the controller. Using this technique, the PLC can be efficiently applied to the several hundred meters different control points such as the ON-OFF control fur the agriculture farm, the building automation system, a multi group of machine control.

• Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.18.1-18.10
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• 2016

In this study, to clarify the function of $PoPLC-{\beta}1$, in response to stress challenge, we examined the $PoPLC-{\beta}1$ expression pattern in response to external stress (pathogen-associated molecular pathogen challenge and environmental challenge including temperature and salinity). $PoPLC-{\beta}1$ expression analysis of tissue from olive flounder showed that the messenger RNA (mRNA) was predominantly expressed in the brain, heart, eye, liver, spleen, and stomach. We also tested the mRNA expression of the $PoPLC-{\beta}1$ in the spleen and kidney of olive flounder by RT-PCR and real-time PCR following stimulation with lipopolysaccharide (LPS), concanavalin A (ConA), or polyinosinic:polycytidylic acid (PolyI:C) and compared with the inflammatory cytokines IL-1b and IL-6 in the stimulated flounder tissues. Each of the spleen and kidney and mRNA transcripts of $PoPLC-{\beta}1$ were increased 30- and 10-fold than normal tissue at 1-6 h post injection (HPI) with PolyI:C when the expression of $PoPLC-{\beta}1$ transcript was similar to LPS and ConA. We also tested the expression of $PoPLC-{\beta}1$ in response to temperature and salinity stress. The expression of $PoPLC-{\beta}1$ also was affected by temperature and salinity stress. Our results provide clear evidence that the olive flounder $PLC-{\beta}1$ signal pathways may play a critical role in immune function at the cellular level and in inflammation reactions. In addition, $PLC-{\beta}1$ appears to act as an oxidative-stress suppressor to prevent cell damage in fish.