• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2013년도 춘계학술대회 논문집
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• pp.325-326
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• 2013

• BMB Reports
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• 제31권1호
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• pp.77-82
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• 1998

To purify the geranylgeranyl protein transferase type I (GGPT-I) efficiently, a gene expression system using the pGEX-4T-1 vector was constructed. The cal1 gene, encoding the ${\beta}$ subunit of GGPT-I, was subcloned into the pGEX-4T-1 vector and co-transformed into E. coli cells harboring the ram2 gene, the ${\alpha}$ subunit gene of GGPT-I. GGPT-I was highly expressed as a fusion protein with glutathione S-transferase (GST) in E. coli, purified to homogeneity by glutathione-agarose affinity chromatography, and the GST moiety was excised by thrombin treatment. The purified yeast GGPT-I showed a dose-dependent increase in the transferase activity, and its apparent $K_m$ value for an undecapeptide fused with GST (GST-PEP) was $0.66\;{\mu}M$ and the apparent value for geranylgeranyl pyrophosphate (GGPP) was $0.071\;{\mu}M$.

• Nuclear Medicine and Molecular Imaging
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• 제40권4호
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• pp.211-217
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• 2006

재조합된 scFv lym-1의 세포결합 실험에서 높은 면역반응성을 보였으며, 비특이 반응에서 모항체인 IgG lym-1에 의해 결합이 억제됨을 확인하였고, 동물 영상을 통해 IgG보다 분자랑이 작은 scFv lym-1 항체가 빠른 체내대사율과 종양섭취율을 보여 방사면역진단에 유용할 것으로 보여진다.

• 한국식물병리학회지
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• 제11권1호
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• pp.66-72
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• 1995

The coat protein (CP) gene of cucumber mosaic virus-As (CMV-As) strain was engineered for expression in the plant by using the cauliflower mosaic virus 35S transcript regulatory sequences. The CP gene was cloned into an Agrobacterium-derived binary vector. A chimeric gene was constructed by the cDNA of CMV-As CP and plant expression vector pBI121. The clone, pCMAS66, was first introduced into the phagemid vector pSPORT1 for situating sense orientation for translation and making restriction sites in order to re-introduce plant expression vector, pHI121. The resulting subclone pCASCP02 and plant expression vector pBI121 were treated with BamHI-SacI for excising the target gene and removing GUS gene, respectively. After Agrobacterium transformation by freeze-thaw technique, the clone, pCMASCP121-123 which contains sense orientation of the target gene, was selected and confirmed by restriction endonuclease analysis. The CMV-As CP gene was introduced into A. tumefaciens. The results on tobacco plant transformation with the vector system revealed that the system could be successfully introduced and showed high frequency of selection to putative transformations.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1996년도 하계학술대회 논문집 A
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• pp.603-605
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• 1996

The SLIM used in the conveyance system has been generally developed the controller based on the slip frequency control and the VVVF method to obtain the quick response for the position control signal. This paper deals with the trust control of the SLIM by vector control with Bang-Bang condition. Also, the control system is composed of the PI controller for soft start of the SLIM and the q-axis current controller for correction in phase with Space Vector for reducing the harmonic pulsation in low speed. The processing for vector control and robust dynamic breaking control is carried out by MC80196KC micro processor and IGBT module. The proposed scheme is verified through the computer simulation and experiments for the 10KW SLIM.

• Journal of the Optical Society of Korea
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• 제20권1호
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• pp.135-139
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• 2016

I propose a vector passive harmonic mode-locked fiber laser based on topological insulator Bi2Se3 interacting with a fiber taper with a diameter of 7 μm. The particles of topological insulator are deposited uniformly onto the fiber taper with light pressure effect. By incorporating the fabricated saturable absorber into an Er-doped fiber laser cavity, stable mode-locked fiber is obtained. Due to the intense evanescent field of the fiber taper, strong confinement of light enhances the nonlinearity of the laser cavity, and passive harmonic mode-locking is performed. I observe a maximum harmonic mode-locking of 356th, corresponding to a frequency of 3.57 GHz. The pulse duration is 824 fs, and the full width at half maximum of the spectrum is about 8.2 nm. The polarization dependent loss of the saturable absorber is ~ 2.5 dB in the wavelength range of the C band. As the cavity contains no other polarization dependent device, the mode-locked laser is functioning in the vector state. The harmonic order vs pump power is investigated. To the best of our knowledge, this report is the highest frequency mode-locked fiber laser based on Bi2Se3. Experimental results indicate that the topological insulator Bi2Se3 functioning with a thin fiber taper is effective for vector harmonic mode-locking.

• 한국측량학회지
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• 제30권6_2호
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• pp.665-672
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• 2012

Amid rapidly increasing imagery inputs and their volume in a remote sensing imagery database, Content-Based Image Retrieval (CBIR) is an effective tool to search for an image feature or image content of interest a user wants to retrieve. It seeks to capture salient features from a 'query' image, and then to locate other instances of image region having similar features elsewhere in the image database. For a CBIR approach that uses texture as a primary feature primitive, designing a texture descriptor to better represent image contents is a key to improve CBIR results. For this purpose, an extended feature vector combining the Gabor filter and co-occurrence histogram method is suggested and evaluated for quantitywise and qualitywise retrieval performance criterion. For the better CBIR performance, assessing similarity between high dimensional feature vectors is also a challenging issue. Therefore a number of distance metrics (i.e. L1 and L2 norm) is tried to measure closeness between two feature vectors, and its impact on retrieval result is analyzed. In this paper, experimental results are presented with several CBIR samples. The current results show that 1) the overall retrieval quantity and quality is improved by combining two types of feature vectors, 2) some feature is better retrieved by a specific feature vector, and 3) retrieval result quality (i.e. ranking of retrieved image tiles) is sensitive to an adopted similarity metric when the extended feature vector is employed.

• 정보통신설비학회논문지
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• 제1권2호
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• pp.19-29
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• 2002

본 논문은 MPEG-21 디지털 아이템 적응의 핵심 기술 중 하나인 혼성 비디오 부호변환에 관해 고찰한다. 부호변환기를 복잡도 측면에서 효율적으로 구현하기 위해서는 움직임 벡터 재사용 기법이 반드시 필요하나, 기존의 방법들은 P-picture만을 위한 움직임 벡터 재사용 기법을 채용하고 있다. 본 논문에서는 B-picture를 위한 두가지 움직임 벡터 재사용 기법을 제안한다. 제안한 기법을 사용함으로써 I/B/P picture 양식으로 부호화되는 MPEG 비트열로 부호변환이 가능하게 된다. 컴퓨터 실험을 통해 본 논문에서 제안한 방법은 부호 변환기의 성능을 일정 범위 내로 보존하면서도, 계산 효율을 크게 향상시킬 수 있음을 보인다.

• KSBB Journal
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• 제5권1호
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• pp.49-58
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• 1990

대장균으로부터 alpha interferon의 생산과 분비를 유도하기 위해 대장균의 lipoprotein promoter, lactose promoter 및 operator와 lipoprotein의 signal seqquence를 가지는 vector에 alpha-IFN유전자를 cloning하여 발현 vector pIF-III-B와 vector pIF-III-C를 제작하였다.

• 전자공학회논문지 IE
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• 제43권4호
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• pp.37-42
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• 2006

본 논문에서는 MPEG2 부호화기내에서 워터마크 세기를 비트율과 움직임 벡터의 크기에 따라 적응적으로 조절하는 방법을 제안한다. I 프레임에 대하여는 양자화 스텝 크기에 따라 워터마크 세기를 가변적으로 조절하고, P, B 프레임은 고효율의 압축이 실현되므로 워터마크의 강인성을 유지하기 위해 비트율과 매크로 블록의 움직임 벡터의 크기에 따라 적응적으로 워터마크 세기를 조절한다. 워터마크 검출은 MPEG 동영상을 완전히 복호화하지 않고 MPEG 복호화시에 DCT 영역에서 실시간으로 검출한다. 실험 결과, 제안한 방법은 화질의 차이가 눈에 띄지 않고 GoP 변환후 동영상 재압축, 저주파 필터 공격과 프레임 삭제 등의 동영상 편집 공격에서도 강인함을 나타낸다.