• The Journal of the Acoustical Society of Korea
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• v.34 no.2
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• pp.171-176
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• 2015

We present a speaker verification method by extracting i-vectors based on dominant state information of Hidden Markov Model (HMM) - Universal Background Model (UBM). Ergodic HMM is used for estimating UBM so that various characteristic of individual speaker can be effectively classified. Unlike Gaussian Mixture Model(GMM)-UBM based speaker verification system, the proposed system obtains i-vectors corresponding to each HMM state. Among them, the i-vector for feature is selected by extracting it from the specific state containing dominant state information. Relevant experiments are conducted for validating the proposed system performance using the National Institute of Standards and Technology (NIST) 2008 Speaker Recognition Evaluation (SRE) database. As a result, 12 % improvement is attained in terms of equal error rate.

• Korean Journal of Microbiology
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• v.32 no.4
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• pp.271-279
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• 1994

To develop a yeast strain which stably secretes both $\alpha$-amylase and glucoamylase and therefore is able to convert starch directly to ethanol, a mouse salivary $\alpha$-amylase cDNA gene with a yeast alcohol dehydrogenase I promoter has been introduced into the cell of a Saccharomyces diactaticus hybrid strain secreting only glucoamylase. To secrete both enzymes more stably without loss of the $\alpha$-amylase gene during a cell-multiplication, an integrating plasmid vector containing $\alpha$-amylase gene was constructed and introduced into the yeast cell. The results showed that the linearized form of the integrating vector was superior in the transformation efficiency and the rate of the expression of the $\alpha$-amylase gene than the circular type of the vector. The yeast transformant having a linearized plasmid vector exhibited higher mitotic stability than the yeast transformant habouring episomat plasmid vector. The transformant containing the linearized vector producing both $\alpha$-amylase and glucoamylase exhibited 2-3 times more amylolytic activity than the original untransformed strain secreting only glucoamylase.

• Journal of Korea Multimedia Society
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• v.10 no.11
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• pp.1427-1438
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• 2007

Video watermarking technologies are classified into types of four kinds. The first type is to embed the watermark into a raw video signal and to code the watermarked video signal. Most of video watermarking technologies fall into the category of this type. The second type is to apply watermarking to the coding process, such as block DCT and quantization. The third is to directly embed the watermark into the compressed bitstream itself. Generally, it is referred as labelling rather than watermarking. Finally, the fourth is to embed the water mark into MPEG motion vector. This type has the difficulty in real-time process because of the high complexity and has the blocking effects because of DCT-based on coder. In this paper, we proposed the digital video watermarking that embed the watermark in SPIHT video code for I-frame using motion vector analysis. This method can remove the blocking effect occurred at the DCT-based on coder and obtain video data that has progressive transmission property. The proposed method is to select the region for the watermark embedding in I frame using motion vector estimated from the previous P or B frame. And then, it is to perform DWT and embed the watermark based on HVS into the wavelet coefficients in the same subband of DWT as the motion vector direction. Finally, the watermarked video bitstream is obtained by the SPIHT coder. The experimental results verified that the proposed method has the invisibility from the objective and subjective image quality and the robustness against the various SPIHT compression and MPEG re-code.

• The KIPS Transactions:PartB
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• v.15B no.6
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• pp.575-584
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• 2008

According to the network condition, the communication network overload could be occurred when media transmitting. Many researches are being carried out to lessen the network overload, such as the filtering, load distributing, frame dropping and many other methods. Among these methods, one of effective method is frame dropping that reduces specified video frames for bandwidth diminution. B frames are dropped and then I, P frames are dropped according to dependency among the frames in frame dropping. This paper proposes a scheme for protecting copyrights by encryption, when we apply frame dropping to reduce bandwidth of media following MPEG-4 file format. We designed two kinds of frame dropping: first one stores and then sends the dropped files and the other drops frames in real-time when transmitting. We designed three kinds of encryption methods in which DES algorithm is used to encrypt MPEG-4 data: macro block encryption in I-VOP, macro block and motion vector encryption in P-VOP, and macro block and motion vector encryption in I, P-VOP. Based on these three methods, we implemented a digital right management solution for MPEG-4 data streaming. We compared the results of dropping, encryption, decryption and quality of video sequences to select an optimal method, and there is no noticeable difference between the video sequences recovered after frame dropping and the ones recovered without frame dropping. The best performance in encryption and decryption of frames was obtained when we apply the macro block and motion vector encryption in I, P-VOP.

• Current Optics and Photonics
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• v.2 no.2
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• pp.134-139
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• 2018

A novel approach for frequency-tripling vector signal generation via balanced detection without precoding and optical filter is proposed. The scheme is mainly utilizing an integrated dual-polarization quadrature phase shift keying (DPQPSK) modulator. In the DPQPSK modulator, one QPSK modulator is driven by an RF signal to generate high-order optical sidebands, while the other QPSK modulator is modulated by I/Q data streams to produce baseband vector signal as an optical carrier. After that, a frequency-tripling 16-quadrature-amplitude-modulation (16QAM) vector millimeter-wave (mm-wave) signal can be obtained by balanced detection. The proposed scheme can reduce the complexity of transmitter digital signal processing. The results show that, a 4 Gbaud baseband 16QAM vector signal can be generated at 30 GHz by frequency-tripling. After 10 km single-mode fiber (SMF) transmission, the constellation and eye diagrams of the generated vector signal perform well and a bit-error-rate (BER) below than 1e-3 can be achieved.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.144-149
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• 1996

To develope the baculovirus expression vector system (BEVS) using Spodoptera exigua nuclear polyhedrosis virus (SeNPV), we characterized the polyhedrin of SeNPV. The SeNPV polyhedra was irregular and composed of the major protein molecular weight of 30 kDa determined by electronmicroscopy and SDS-AGE analysis, respectively. The nucleotid suquences of 876 bases including the coding region of polyhedrin gene was determined and it was revealed that the polyhedrin gene is located within Xho I 3.0Kb and Nco I 6.0 Kb by Southern blot analysis, respectively. Also, the Xho I 3.0 Kb and the Nco I 6.0 Kb fragments were cloned and restriction enzyme map of these clones were determined.

• Proceedings of the KIPE Conference
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• 2009.11a
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• pp.271-273
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• 2009

Because few signals are generated using low resolution position sensor at low speed, rotor position estimation is difficult in drum washing machine application. Besides, inertia of water and drum affect the initial operation load up to two times of rated load. Therefore overcurrent can be occurred at initial operation. In this paper, IPMSM vector control for drum washing machine is proposed by applying I/F initial operating method and sensorless control. And we verify the performance through experiment.

• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.247-254
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• 2015

In this presentation, I describe the expression and purification of the recombinant liver X receptor β-ligand binding domain proteins in E. coli using a commercially available double cistronic vector, pACYCDuet-1, to express the receptor heterodimer in a single cell as the soluble form. I describe here the expression and characterization of a biologically active heterodimer composed of the liver X receptor β-ligand binding domain and retinoid X receptor α-ligand binding domain. Although many of these proteins were previously seen to be produced in E. coli as insoluble aggregates or "inclusion bodies", I show here that as a form of heterodimer they can be made in soluble forms that are biologically active. This suggests that co-expression of the liver X receptor β-ligand binding domain with its binding partner improves the solubility of the complex and probably assists in their correct folding, thereby functioning as a type of molecular chaperone.

• Journal of applied mathematics & informatics
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• v.11 no.1_2
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• pp.1-57
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• 2003

Vector fields in three-space admit bundles of internal variables such as a Heisenberg algebra bundle. Information transmission along field lines of vector fields is described by a wave linked to the Schrodinger representation in the realm of time-frequency analysis. The preservation of local information causes geometric optics and a quantization scheme. A natural circle bundle models quantum information visualized by holographic methods. Features of this setting are applied to magnetic resonance imaging.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.588-591
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• 2000

Serine palmitoyl transferase(SPT) and ceramidase are the key enzymes in sphingolipid biosynthesis. To study sphingolipid metabolism, we have got the 5'-upstream regions of human serine palmitoyl transferase subunit II and acid ceramidase gene by using GenomeWalker kits(Clontech Co.). Human genomic DNA was purified from HT29, human colon canser cell line by using DNAzol. We got several bands after secondary PCR and subcloned them to T7bule vector. Human SPTII promoter which we got was 2690bp but we cut it with Bgl II and vector with Bgl II and BamH I, and subcloned 1782bp to pGL2-enhancer vector and pGL2-basic vector with luciferase reporter gene. Human acid ceramidase promoter which we got were 2028bp and 1034bp and subcloned to pGL2-enhancer vector and pGL2-basic vector. We transfected these promoters to HT29 cell and assayed luciferase activity. For measuring transfection efficiency, pRL-TK vector with seapancy luciferase reproter gene was cotransfected with these promoters.