• 한국식품과학회지
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• 제39권4호
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• pp.452-457
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• 2007

본 실험은 백합의 생리활성 기능을 탐색하여 기능성 식품의 원료로 활용하기 위해 구근, 잎, 꽃을 3부분으로 구분하여 부위별 추출물의 총 페놀함량, 전자 공여능, SOD 유사활성, hydroxyl radical scavenging activity 및 항균성을 측정하였다. 백합 부위별 총 페놀함량에서는 L. davidii 잎 0.068 mg/mL, L. lancifolium 구근 0.039 mg/ml로 나타났다. 플라보노이드 함량은 L. davidii 잎 0.72 mg/mL, L. lancifolium 구근 0.65 mg/ml로 측정되어 백합 품종 및 부위에 따라 페놀성 화합물의 함량에 큰 차이가 있었다. 백합 에탄올 추출물의 SOD 유사활성 측정결과 L. davidii 의 잎 82.7 ${\pm}$ 2.8%, 꽃 66.1 ${\pm}$ 6.7% 구근 50.6 ${\pm}$ 2.0%로 잎에서 가장 높았다. L. lancifolium 에탄올 추출물은 잎 74.9 ${\pm}$ 3.5%, 꽃 73.9 ${\pm}$ 4.1% 및 구근 60.0 ${\pm}$ 4.7%로 나타났다. 백합 부위별 에탄올 추출물의 hydroxyl radical 소거능 측정결과 L. davidii 에탄올 추출물의 소거능은 잎 81.8 ${\pm}$ 2.7%, 꽃 81.4 ${\pm}$ 4.6%, 구근 80.9 ${\pm}$ 7.1% 이었고 L. lancifolium 추출물은 잎 76.4 ${\pm}$ 4.6%, 꽃 81.4 ${\pm}$ 4.6%, 구근 84.5 ${\pm}$ 1.2%로 나타나 품종별, 부위별로 hydroxyl radical 소거능에 큰 차이는 나타나지 않았다. L. davidii 추출물의 전자 공여능 측정 결과 잎 24.9 ${\pm}$ 5.6%, 꽃 14.9 ${\pm}$ 0.4%, 구근 13.798 ${\pm}$ 8.0% 이었고 L. laneifolium 추출물은 잎 15.1 ${\pm}$ 0.7%, 꽃 17.3 ${\pm}$ 0.0%, 구근 13.3 ${\pm}$ 0.2%의 순으로 나타났다. 또한 백합 부위별 추출물을 이용한 그램 양성 균주에서 항균효과 측정 결과는 B. subtilis와 S. enteritidis 에서도 L. davidii, L. lancifolium 의 잎과 꽃에서 10 mm 이상의 clear zone을 형성하였고, 특히 B. subtilis에서는 L. lancifolium 의 꽃 부위에서 항균효과가 높았다. 또한 Gram 음성 균주에서는 Listeria monocytogenes 및 E coli 에서 L. davidii, L. laneifolium의 잎과 꽃에서 10mm 이상의 clear zone을 형성하였으며, L. davidii의 꽃과 L. laneifolium의 꽃, 잎에서 12mm 이상의 높은 항균효과를 나타내었다.

• 한국식품저장유통학회지
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• 제15권3호
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• pp.325-331
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• 2008

The propose of this study was to investigate the antioxidant activity of 80% ethanol extracts and various solvent fractions of dandelion (Taraxacmn officinale) leaves and roots, Total phenolics and phenolic acid contents were also examined. The total phenol content of leaves and roots were $7.9{\pm}0.4%$ and $9.4{\pm}0.3%$ respectively. Eight phenolic acids were separated by GC, among which caffeic acid (113.7 mg%)and m-coumaric acid (152.6 mg) were the dominant phenolic acids in leaves and roots, respectively. Amongst solvent functions of leaves and roots, the ethyl acetate fraction showed the strongest radical scavenging activity. A strong correlation was found between total phenol content and electron-donating ability, and ABTS radical scavenging activity showed a similar trend as electron-donating ability. Hydroxyl-radical-scavenging activity and lipid peroxidation were significantly higher in the ethyl acetate fraction than other factions. In particular, the SOD-like activity was highest (43.6%) in the ethyl acetate fraction of dandelion leaves, and was higher than that of trolox. Thus, the ethyl acetate fraction of dandelion leaves exhibited significant phenol content, antioxidant activity, and free-radical-scavenging effects.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1523-1527
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• 2009

The present study was to investigate the in vitro antioxidant potential of hot water extract and its fractions from dichloromethane ($CH_2Cl_2$), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water ($H_2O$) of castor-aralia (Kalopanax pictus) leaves using different antioxidant tests. Among these crude extract and fractions, EtOAc fraction exhibited higher antioxidant potency than others in 1,1-diphenyl-2-pricylhydrazyl (DPPH) free radical scavenging, reducing power assay, and reactive oxygen species (ROS) scavenging activity. However, $CH_2Cl_2$ fraction showed higher hydroxyl radical scavenging and DNA damage protective activity. This work demonstrates the potential of castor-aralia leaves as antioxidant functional food ingredients.

• 한국식품영양학회지
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• 제34권3호
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• pp.289-294
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• 2021

The antioxidant activity and α-glucosidase inhibitory activity of the solvent fraction fractionated from the methanol extract of Saururus chinensis Baill were examined. As a result of measuring the yields of methanol, hexane, chloroform, ethylacetate, butanol, and water fractions, the extraction yield of fraction was 18.60, 3.38, 24.03, 7.75, 8.11 and 62.57%, respectively. The total polyphenol content of the methanol extract of Saururus chinensis Baill was 13.40, 4.62, 7.39, 31.24, 25.76 and 5.64 mg GAE/g, respectively. DPPH radical scavenging activity (IC_50%) results were 20.81, 5.47, 10.15, 22.63, 19.68 and 21.06 ug/mL, respectively, and hydroxyl radical scavenging activity (IC_50%) results were 15.81, 2.69, 8.84, 12.80, 3.70 and 3.39 ug/mL. Hydrogen peroxide scavenging activity scavenging activity measurement (IC_50%) showed 33.63, 8.88, 16.93, 32.84, 33.79, and 33.71 ug/mL in methanol, hexane, chloroform, ethyl acetate butanol, and water fractions, respectively. The α-glucosidase inhibitory activity of the solvent fraction fractionated with the methanol extract of 300 sec was measured for the α-glucosidase inhibitory activity of methanol, hexane, chloroform, ethyl acetate butanol, and water fraction, respectively, 15.85, 10.84, 15.74, 24.90, 2.58 and 35.70%.

• 동의신경정신과학회지
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• 제17권1호
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• pp.1-16
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• 2006

Objective: An water extract of the Hwang-Ryun-Chung-Sim-Um (HRC) was assessed to determine the mechanisms of its antioxidant activity. In addition, the HRC was examined in vitro for the inhibitory effect on the acetylcholinesterse (AChE). Methods: The HRC exhibited a concentration-treatment; scavenging ${\alpha},{\alpha}-diphenyl-{\beta}-picrylhydrazyl$ (DPPH) radical, linoleic acid oxidation in a thiocyanate assay system, hydroxyl radical-induced DNA nicking. We investigated mRNA levels such as catalase activity, superoxide-dismutase and glutathione peroxidase. The water extract of HRC showed inhibitory effect on AChE activity. Result: The HRC extract showed dose-dependent free radical scavenging activity, including DPPH radicals and hydroxyl radicals, using different system. The HRC was also found to be effective in protecting plasmid DNA against the strand breakage induced by Hydroxyl radicals in Fenton's reaction mixture. Futhermore, catalase mRNA expression levels increased, but SOD1 and MnSOD was not expressed. HRC in a various concentration-dependent decreased AChE mRNA levels and inhibitory effect showed AChE. Conclusion: According to the above results, it is supposed that HRC is applicable to the Dementia-type of Alzheimer clinically.

• 한국식품영양학회지
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• 제30권5호
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• pp.1127-1131
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• 2017

당조고추의 기능성 소재로서의 가능성을 알아보기 위하여 당조고추 70% 에탄올 추출물에 대한 항산화 활성 및 항암활성을 측정하였다. 당조고추 70% 에탄올 추출물의 총 폴리페놀은 30.29 mg GAE/g extract로 나타났다. 당조고추 70% 에탄올 추출물에 대한 DPPH radical 및 hydroxyl radical 소거활성을 $IC_{50%}$ 측정한 각각 2.87, 10.55 mg/mL의 소거활성을 보여 주었으며, ${\alpha}$-glucosidase 및 ${\alpha}$-amylase 저해활성은 10 mg/mL의 농도에서 각각 35.67, 58.41%로 나타났다. 당조고추 70% 에탄올 추출물에 대한 항암활성은 대장암 세포주의 0.5 mg/mL 농도에서 50% 암세포 성장억제를 보였지만, 다른 암세포에서는 억제효과를 나타내지 않았다.

• Journal of Microbiology and Biotechnology
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• 제28권1호
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• pp.1-11
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• 2018

An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structure-activity relationship in these peptides. To prepare antioxidant peptides better and improve their stability, the design and expression of an antioxidant peptide from Pinctada fucata (named DSAOP) was studied. The peptide contains a common precursor of an expression vector containing an ${\alpha}$-helix tandemly linked according to the BamHI restriction sites. The DNA fragments encoding DSAOP were synthesized and subcloned into the expression vector pET-30a (+), and the peptide was expressed mostly as soluble protein in recombinant Escherichia coli. Meanwhile, the DPPH radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging activity of DSAOP $IC_{50}$ values were $0.136{\pm}0.006$, $0.625{\pm}0.025$, and $0.306{\pm}0.015mg/ml$, respectively, with 2-fold higher DPPH radical scavenging activity compared with chemosynthesized AOP (p < 0.05), as well as higher superoxide radical scavenging activity compared with natural AOP (p < 0.05). This preparation method was at the international advanced level. Furthermore, pilot-scale production results showed that DSAOP was expressed successfully in fermenter cultures, which indicated that the design strategy and expression methods would be useful for obtaining substantial amounts of stable peptides at low costs. These results showed that DSAOP produced with recombinant Escherichia coli could be useful in cosmetic skin care products, health foods, and pharmaceuticals.

• 한국식품영양과학회지
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• 제40권6호
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• pp.804-808
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• 2011

본 연구는 염생식물이라는 특성으로 인해 갯벌이 발달한 우리나라에서 쉽게 접할 수 있는 칠면초의 항산화활성을 검토하고자 이루어졌다. 70% ethanol로 추출한 추출수율은 9.74%였으며 이를 hexane, chloroform, ethyl acetate, butanol, water로 순차 분획한 수율은 water 층이 원물대비 8.73%로 가장 높은 수율을 보였고 대체적으로 극성이 높을수록 높은 분획 수율을 나타내었다. 하지만 분획물간 총 페놀과 총 플라보노이드 함량 측정 결과 ethyl acetate 분획물의 에서 가장 높게 측정되었다. Ethyl acetate 분획물의 항산화력을 hydroxyl radical 소거능, hydrogen peroxide 소거능, xanthine oxidase 소거능 등을 측정하여 합성항산화제인 BHA와 비교한 결과 BHA의 80~90% 가량의 활성을 보여주었다.

• Food Science and Biotechnology
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• 제17권3호
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• pp.501-507
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• 2008

The quantitative structure-activity relationships (QSAR) study of antioxidative anthocyanidins and their glycosides were evaluated using 4 different assays of Trolox equivalent antioxidant capacity (TEAC), superoxide radical ($O_2^{{\cdot}-}$), hydrogen peroxide ($H_2O_2$), and peroxynitrite radical ($ONOO^-$) scavenging with TSAR software. Four models were developed with significant predictive values ($r^2$ and p value), which indicated that the antioxidant activities were mainly governed by the 3-dimensional structural energy (torsional energy), constitutional properties (the number of hydroxyl and methyl groups), and electrostatic properties (heat of formation, and dipole, quadrupole, and octupole components). This QSAR approach could contribute to a better understanding of structural properties of anthocyanidins and their glycosides that are responsible for their antioxidant activities. It might also be useful in predicting the antioxidant activities of other anthocyanins.

• 한국약용작물학회지
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• 제22권6호
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• pp.442-448
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• 2014

The major objective of this study was to investigate the usability as cultivar for processing of new Citrus hybrid 'Hamilgam'. We investigated various antioxidant activities, such as DPPH radical, ABTS radical, Hydroxyl radical and Superoxide anion radical effect, along with total polyphenol content and total flavonoid content of Citrus hybrid 'Hamilgam', C. unshiu, C. natsudaidai. The total polyphenol content and total flavonoid content were the highest in the Hamilgam. The major flavonoids of Hamilgam and Natsudaidai were naringin and neohesperidin, and those of Unshu were narirutin and hesperidin, as determined by HPLC. Especially, the neohesperidin content of Hamilgam showed much higher (100 times over) than that of Unshu. However, the aglycone form was not found. DPPH and ABTS radical scavenging activities were the highest in Hamilgam peels. Hydroxyl radical scavenging activity was high in the order of Unshu, Hamilgam and Natsudaidai. Superoxide anion radical scavenging of 3 cultivars peels displayed low activities compared to DPPH and ABTS radical scavenging. Based on these results, Hamilgam peel extracts possess antioxidant activities and may thus serve as potential sources of functional food, cosmetic products, etc.