• Title/Summary/Keyword: human-engineering

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Mechanisms of Suppression of Matrix Metalloproteinases in UVB-Irradiated HaCaT Keratinocytes of Colored Rice Varieties (UVB에 조사된 HaCaT Keratinocytes에서의 유색미에 의한 Matrix Metalloproteinases 발현억제 메커니즘)

  • Choi, Eun-Young;Lee, Jae-Bong;Kim, Do-Hoon;Kwon, Yong-Sham;Cheon, Jung-Yoon;Lee, Jin-Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.5
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    • pp.562-571
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    • 2017
  • In this study, we investigated the anti-oxidant activities [electron-donating ability (EDA), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging ability, and reactive oxygen species (ROS) inhibitory activity], anti-wrinkle activities [collagenase inhibitory activity, suppression and/or phosphorylation of matrix metalloproteinases (MMPs), and mitogen-activated protein (MAP) kinase activity], and mRNA expression levels using reverse transcription-polymerase chain reaction (RT-PCR) assay in ultraviolet (UV) B ray ($50mJ/cm^2$)-irradiated human keratinocyte HaCaT cells. Josaengheugchal, Sinneungheugchal (SE), Shintoheug rice, Heugjinju rice, and Heugseol (HE) among colored rice varieties were reported to have excellent antioxidant properties. In the EDA and ABTS radical scavenging assays, extracts of the five colored rice varieties had scavenging activities of 72% at concentrations higher $50{\mu}g/mL$. In the collagenase inhibition assay, ethanol extracts of the five colored rice varieties showed high inhibitory effects of about 60% at concentrations higher $25{\mu}g/mL$. In the ROS inhibition assay, ethanol extracts of HE and SE showed very excellent inhibition efficacies at all concentrations. We determined molecular biological mechanisms of MMPs (MMP-1, -3, -8, and -13) and mitogen-activated protein kinase (MAPK) with HE, and the results show that HE suppressed expression of MMPs and phosphorylation of MAPK and increased expression of pro-collagen type I in UVB-irradiated cells. It was also confirmed by RT-PCR that HE reduced expression of MMPs mRNA. Therefore, these results suggest that HE has anti-wrinkle and collagen production effects and may be used as a material in the development of functional food and cosmetic industries.

Effects of Mulberry Leaves Powder on Lipid Metabolism in High Cholesterol-Fed Rats (뽕잎분말이 고콜레스테롤 식이 투여 흰쥐의 지질대사에 미친 영향)

  • Kim, Ae-Jung;Kim, Sun-Yeou;Choi, Mi-Kyeong;Kim, Myung-Hwan;Han, Myung-Ryun;Chung, Kun-Sub
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.636-641
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    • 2005
  • Mulberry leaves, high in dietary fiber and some nutritional materials, are thought to have hypocholesterolemic effect. Therefore, effect of mulberry leaf powder on serum lipid profiles were studied using rats with diet-induced hypercholesterolemia. Male Sprague-Dawley rats were fed AIN-93 diet (control group), and diets containing high-cholesterol and 0% mulberry leaves powder, high-cholesterol and 5% mulberry leaves powder, and high-cholesterol and 10% mulberry leaves powder for 4 weeks. Hypercholesterolemia was induced by adding 1% cholesterol and 0.5% cholic acid to all diets except in control group. Although no differences were observed in food intake and initial body weight among groups, mulberry leaf treatment resulted in significant decreases in food efficiency ratio and body weight gain. Mulberry leaf treatment decreased serum lipid profiles, atherogenic index, cardiac risk factor, low density lipoprotein cholesterol ratio, serum aspartate transaminase, and liver lipid levels. High density lipoprotein cholesterol, total cholesterol, serum HDL-cholesterol, and fecal lipid levels increased, suggesting mulberry leaves could improve hyperlipidemia and liver action, thereby proventing cardiovascular disease.

Significance Analysis of Facility Fires Though Spatial Econometrics Assessment (공간계량분석 방법에 따른 시설물 화재 발생 유의성 분석)

  • Seo, Min Song;Yoo, Hwan Hee
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.38 no.3
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    • pp.281-293
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    • 2020
  • Recently, large and small fires have been happening more often in Korea. Fire is one of the most frequent disasters along with traffic accidents in korean cities, and this frequency is closely related to the land use and the type of facilities. Therefore, in this study, the significance of fires was analyzed by considering land use, facility types, human and social factors and using 10 years of fire data in Jinju city. Based on this, OLS (Ordinary Least Square) regression analysis, SLM (Spatial Lag Model) and SEM (Spatial Error Model) using space weights, were compared and analyzed considering the location of the fire and each factor, then a statistical model with high suitability was presented. As a result, LISA analysis of spatial distribution patterns of fires in Jinju city was conducted, and it was proved that the frequency of fires was high in the order as follow, central commercial area, industrial area and residential area. Multiple regression analysis was performed by integrating demographic, social, and physical variables. Therefore, the three models were compared and analyzed by applying spatial weighting to the derived factors. As a result of the significance test, the spatial error model was analyzed to be the most significant. The facilities that have the highest correlation with fire occurrence were second type neighborhood facilities, followed by detached house, first type neighborhood facilities, number of households, and sales facilities. The results of this study are expected to be used as significant data to identify factors and manage fire safety in urban areas. Also, through the analysis of the standard deviation ellipsoid, the distribution characteristics of each facility in the residential area, industrial area, and central commercial area among the use areas were analyzed. In, the second type neighborhood facility with the highest fire risk was concentrated in the center. The results of these studies are expected to be used as useful data for identifying factors and managing fire safety in urban areas.

Is the UU Stitch Really Alternative to Modified MA (Mason-Allen) Stitch for Rotator Cuff Repair? - Biomechanical Comparative Study of UU to Modified MA Stitch - (회전근 개 파열의 봉합에서 UU 봉합법은 변형된 MA(Mason-Allen) 봉합법을 대치할 수 있는가? - UU 봉합법과 변형된 MA 봉합법의 생역학적 비교-)

  • Friedman, Darren J;Ko, Sang-Hun;Park, Ki-Bong;Jun, Hyung-Min;Kim, Tae-Won;Lim, Hyun-Woo;Yum, Young-Jin
    • Clinics in Shoulder and Elbow
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    • v.12 no.2
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    • pp.207-214
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    • 2009
  • Purpose: In arthroscopic rotator cuff repairs there are generally weak link in tendon suture interface, arthroscopic rotator cuff repairs can have higher retear rates than open repairs. The purpose of this study was to compare the strength of UU (Ulsan University) suture than open modified MA (Mason-Allen) suture when suture anchored into bone. Materials and Methods: The human supraspinatus tendons were harvested from the shoulder of the cadaver and split in 2 times, producing four tendons per one shoulder, for a total of 24 specimens. Two suture configurations (UU, MA) were randomized and checked on each set of tendons. Specimens were cyclically loaded under force control between 5 and 30 N at 0.25 Hz for fifty cycles. Each specimen was loaded to failure under displacement control at 1 mm/sec. Cyclic elongation, peak to peak displacement, stiffness, ultimate tensile load, mode of failure were checked. Results: No significant difference was found between two suture configuration with respect to peak to peak displacement, cyclic elongation, and stiffness. With regard to ultimate failure load, there were no significant difference statistically between the UU suture and modified MA suture (109.4 N, 110.6 N). The most common mode of failure between both sutures was suture pull-out through the tendon. Conclusion: The UU suture and modified MA suture produced similar biomechanical properties.

A Study on the Correlation Analysis of EEG and Vibraimage due to Auditory and Olfactory Stimulation (청각 및 후각자극에 의한 뇌파(EEG)와 진동이미지기술의 상관성 분석에 관한 연구)

  • Kim, Jung-Min;Kim, Myung-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.6
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    • pp.4291-4297
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    • 2015
  • EEG has been used to measure the emotion of amenity and discomfort in the interior space. EEG is limited to the experiment, because it is a equipment of contact type. However, Vibraimage can measure the emotion with a web camera. Because Vibraimage is a equipment of non-contact type, it is more suitable for the interior space than EEG. Therefor, it tries to find a correlation variable between EEG and Vibraimage to measure the human emotions. In this study, it were analyzed correlation of EEG and vibraimage due to variation of loudness 60[dB], 90[dB] and rosemary, jasmine scents. Check the health status of subjects who were selected 3 male students, and the period of this experiment was about months. The condition of the environmental test room was in temperature 25[$^{\circ}C$], relative humidity 50[RH%], air current speed 0.02[m/s] and illuminance 1000[lux]. It were analyzed correlation of twenty-three index of EEG(absolute ${\theta}$, relative ${\theta}$, absolute $S{\alpha}$, relative $S{\alpha}$, absolute ${\alpha}$, relative ${\alpha}$, absolute ${\beta}$, relative ${\beta}$, absolute $\gamma$, relative $\gamma$, absolute $F{\alpha}$, relative $F{\alpha}$, absolute SMR, relative SMR, $SMR/{\theta}$, $SMR+M{\beta}/{\theta}$, absolute $H{\beta}$, relative $H{\beta}$, $H{\beta}/{\alpha}$, absolute $M{\beta}$, relative $M{\beta}$, SEF50, ASEF50) and ten index of Vibraimage(Aggression, Stress, Tension/Anxiety, Suspect, Balance, Charm, Energy, Self regulation, Inhibition, Neuroticism). As a result, I was found that relative ${\gamma}$ index of EEG and neuroticism index of Vibraimage have a high correlation as (${\pm}$).414 and (${\pm}$).424.

Influence of the curing time for the adhesive on the oxygen-inhibited layer thickness and the shear bond strength to dentin (광조사 시간이 접착제의 표면 미중합층의 두께와 전단접착강도에 미치는 영향에 관한 연구)

  • Choi, Yong-Hoon;Bae, Ji-Hyun;Son, Ho-Hyun;Lee, In-Bog;Um, Chung-Moon;Baek, Seung-Ho;Kim, Oh-Young;Kim, Chang-Keun;Cho, Byeong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.29 no.2
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    • pp.177-184
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    • 2004
  • Objectives : This study investigated the hypothesis that increasing light-curing time would leave the oxygen-inhibited layer (OIL) of the adhesive thinner, and in turn, result in lower shear bond strength (SBS) than those obtained by the routine curing procedures. Methods:120 human extracted posterior teeth were randomly divided into three groups for bonding with three adhesives:All Bond 2/sup (R)/, One Step/sup (R)/, and Adper Prompt/sup (R)/. They were subsequently divided into four subgourps with different light-curing time (10, 20, 30 and 60s). The assigned adhesives were applied on superficial occlusal dentin according to the manufacturer's instructions and cured with one of the four curing times. Composite resin cylinder, 2.35㎜ in diameter, were built on the cured adhesive and light-cured for 40s. SBS were measured after 24h from the bonding using a universal testing machine (crosshead speed 1.0 ㎜/min). The relative thickness of the OIL and the degree of conversion (DC) were determined from the adhesive on a slide glass using FT-NIR in an absorbance mode. Data were analysed with One-way ANOVA and Duncan's multiple test (p〈0.05), Results:With increasing cure time, although there were no significant difference in th SBS of One-step and Adper Prompt (p〉0.05), those of All Bond 2 decreased significantly (p〈0.05). The relative thicknesses of the OIL on each adhesive were not affected by the cure time (p〉0.05). Although the DC of All-Bond 2 were statistically not different with increasing cure time (p〉0.05), those of One-Step and Adper Prompt showed an increasing trends with increasing cure time (p〈0.05). Conclusions:Increasing light-curing time did not affect on the relative thickness of the OIL of the adhesives, and in turn, on the SBS to dentin.

Cellular Protective Effects of Peanut Sprout Root Extracts (땅콩나물 뿌리 추출물의 세포 보호 효과)

  • Jo, Na Rae;Park, Chan Il;Park, Chae Won;Shin, Dong Han;Hwang, Yoon Chan;Kim, Yong Hyun;Park, Soo Nam
    • Applied Chemistry for Engineering
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    • v.23 no.2
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    • pp.183-189
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    • 2012
  • In this study, the cellular protective effect and antioxidative property of peanut sprout root extracts were investigated. Cellular protective effects of peanut sprout root extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction of extracts exhibited a cellular protective effect in a concentration dependent manner. Particularly, the aglycone fraction of extracts showed prominent cellular protective effects in a concentration range (5~50 ${\mu}g/mL$). They are more effective than that of (+)-${\alpha}$-tocopherol, known as a lipid peroxidation chain blocker. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of peanut sprout root extracts on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction of extracts ($OSC_{50}$; 1.59 ${\mu}g/mL$) showed a similar ROS scavenging activity compare with that of L-ascorbic acid (1.50 ${\mu}g/mL$), known as a strong antioxidant. On the other hand, the order of free radical (1,1-diphenyl-2-picrylhydraxyl, DPPH) scavenging activity ($FSC_{50}$) was (+)-${\alpha}$-tocopherol > 80% MeOH extract > aglycone fraction > ethyl acetate fraction. These results indicate that peanut sprout root extracts can function as an antioxidant in biological systems, particularly skin exposed to solar UV radiation by scavenging $^1O_2$ and other ROS, and to protect cellular membranes against ROS.

A survey of the Necessity and Perceptions of Character Education of Health Science and Non-health Science University Students (일개 보건계열 및 비보건계열 학생들의 인성교육에 대한 필요성 및 인식도 조사)

  • Choi, Yong-Keum;Oh, Tae-Jin;Lee, Hyun;Lim, Kun-Ok;Hong, Ji-Heon;Kim, Eun-Jeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.8
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    • pp.344-351
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    • 2019
  • The purpose of this study was to obtain the basic data for developing more advanced courses on character education by surveying and analyzing the perception and demands of character education of university students and further, to provide useful information for creating institutional protocol on character education. The study was conducted from April 2018 to May 2018 on students attending the departments of non-health science and health science university students. A total of 206 students participated in this study, and all the students in the non-health science and health science departments were found to be highly aware of the need for character education, its importance and the possibility of personality development through learning. Students from all the departments showed high levels on average in terms of self-understanding according to their personality abilities, and especially their high levels of 'consideration' and 'responsibility'. For the differences in perception of self-efficacy, the lowest level of recognition was for 'will' and the average values were not high. In their response to personality level, all students answered that their personality was 'high' (42.1%), and that the personality education courses at the schools they are currently attending were 'not satisfied' with both the non-health science and health science students. As a result, there were higher results overall for the health science students than that for the non-health science students, but there were not many significant differences. To this end, education institutes will have to prepare conditions for university students to cultivate their expertise in character, while at the same time helping them grow into human beings with the qualities demanded by society. In addition, the government should establish curriculums and content by accurately identifying the needs of character education and devising concrete measures for their implementation, and by more faithfully considering quantitative and qualitative context types for the content base of character education.

Antioxidant, Antimicrobial and Cellular Protective Effects of Lycopus lucidus Extract and Fraction (택란 추출물 및 분획물의 항산화, 항균 및 세포 보호 효과)

  • Lee, Jin Kyoung;Park, Young Min;Lee, Sang Lae;Song, Ba Reum;Lee, Yun Ju;Park, Soo Nam
    • Applied Chemistry for Engineering
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    • v.30 no.1
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    • pp.114-121
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    • 2019
  • In this study, antioxidative, antibacterial and cytoprotective effects of the ethanol extract and ethylacetate fraction of Lycopus lucidus (L. lucidus) were compared and analyzed. Free radical scavenging activities ($FSC_{50}$) of the L. lucidus extract and fraction were found to be 65.1 and $64.9{\mu}g/mL$ respectively. In the $Fe^{3+}-EDTA/H_2O_2$ system, the reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) for the extract and fraction were 6.6 and $6.3{\mu}g/mL$, respectively which showed excellent total antioxidant abilities. The extract showed antibacterial activity against S. aureus, while the fraction showed in all the bacteria except for A. niger. The cytoprotective effect of L. lucidus extract was compared to that of the fraction and the effect against $^1O_2$-induced cellular damage of human erythrocytes (${\tau}_{50}$) was 51.3 and 73.7 min at $50{\mu}g/mL$, respectively. For the cytoprotective effect of keratinocytes damaged by $H_2O_2$ and UVB, the extracts did not show any efficacy but showed efficacy at $1-2{\mu}g/mL$, respectively. The fraction increased the cell viability up to 85.8 and 81.9%, respectively. As a result of intracellular ROS scavenging activity, the scavenging activity was observed at $1-2{\mu}g/mL$ of the fraction. From the results comparing the physiological activities of L. lucidus extract and the fraction, the ethylacetate fraction of L. lucidus has antioxidative effect similar to that of the extract whereas superior antimicrobial and cytoprotective effects than that of the extract. Overall, the ethylacetate fraction of L. lucidus protects cells from an external stress which can be used as a potential cosmetic material.

Antibacterial, Antioxidative and Antiaging Effects of Allium cepa Peel Extracts (양파껍질 추출물의 항균, 항산화 및 항노화 효과에 관한 연구)

  • Kim, Jung Eun;Kim, A Reum;Kim, Min Ji;Park, Soo Nam
    • Applied Chemistry for Engineering
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    • v.22 no.2
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    • pp.178-184
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    • 2011
  • In this study, the antibacterial, antioxidative and inhibitory effects of Allium cepa peel extracts on tyrosinase and elastase were investigated. MIC values of the ethyl acetate fraction of Allium cepa peel on especially, S. aureus among the skin resident flora (Staphylococcus aureus, S. aureus; Propionibacterium acnes, P. acnes; Pityrosporum ovale, P. ovale; Escherichia coli, E. coli) were 0.06%. The aglycone fraction showed more excellent free radical (1,1-diphenyl-2-picrylhydrazyl radical, DPPH) scavenging activity ($FSC_{50}=5.05{\mu}g/mL$). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the ethyl acetate fraction and aglycone fraction in the luminol-dependent $Fe^{3+}-EDTA/H_2O_2$ system were 0.05 and $0.03{\mu}g/mL$, respectively. The cellular protective effect of the aglycone fraction on the rose-bengal sensitized photohemolysis of human erythrocytes exhibited more prominent (${\tau}_{50}$, 480 min at $25{\mu}g/mL$). The inhibitory effects ($IC_{50}$) of the ethyl acetate fraction and aglycone fraction on tyrosinase were 9.16 and $8.68{\mu}g/mL$, the inhibitory effect ($IC_{50}$) of the aglycone fraction on elastase was $14.12{\mu}g/mL$ The transepidermal water loss of the cream containing 0.1% ethyl acetate fraction was decreased from $8.3g/m^2h$ in control to $6.8g/m^2h$ in the subjects applied with cream containing the ethyl acetate fraction. These results indicate that extract/fractions of Allium cepa peel can function as antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS, and possibly as antiaging agents. Allium cepa peel extract could be used as a new cosmeceutical for whitening and anti-wrinkle products.