• Biomolecules & Therapeutics
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• 제16권3호
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• pp.286-292
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• 2008

Strawberry is widely consumed in diet and has been attracted much attention due to its potential for human health benefits. Strawberry contains a diverse range of phytochemicals but the biological activities with molecular mechanisms are poorly elucidated yet. In this study, the effects of the extracts of strawberry (Maehyang cultivar) on antioxidant, antiinflammatory, and antiproliferative potential against various cancer cells were investigated. The strawberry extracts (SE) of Maehyang cultivar showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activities. In addition, SE inhibited the growth of human colon (HCT-116), lung (A549), stomach (SNU-638) and fibrosarcoma (HT-1080) cancer cells. The strawberry extracts also exhibited the inhibitory effect on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production and suppressed LPS-induced inducible nitric oxide synthase (iNOS) protein and mRNA expression in mouse macrophage RAW 264.7 cells. These findings suggest that the strawberry extracts (Maehyang cultivar) might have antioxidant, antiinflammotry, and anticancer activities.

• 한국축산식품학회지
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• 제22권4호
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• pp.348-351
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• 2002

유황오리 추출물의 각종 암세포에 대한 생육억제효과를 MTT assay를 이용하여 검토한 결과 10 mg/$m\ell$의 농도에서 KB(구강상피암) 89.5$\pm$0.7%, SNU-1(위암) 69.8$\pm$1.7%, K-562(백혈병) 79.8$\pm$2.8%, Farrow(흑색종) 82.7$\pm$2.6%, WiDr 결장암) 76.3$\pm$2.5%, SK-MES-1(폐암) 59.2 $\pm$4.4%, HL6O(백혈병) 60.5 +3.5%, Calu-3(폐암) 53.2$\pm$1.6%, HEP-2(후두암) 80.7$\pm$0.5%, P388(마우스 백혈병) 79.9$\pm$3.7%, 3LL(마우스 폐암) 87.2$\pm$3.3%의 효과가 있다는 사실이 판명되었다. 그리고 유황오리 추출물의 HP-20 column chromatography에서는 100% methyl alcohol 용출물이 HEP-2에 대한 생육억제효과가 있었으며, 10 mg/$m\ell$에서 99.1$\pm$0.4%의 생육억제효과가 나타났다. 또한 일반오리 추출물과 유황오리 추출물의 각종 암세포에 대한 생육억제효과를 비교하였을 때 거의 모든 암세포에서 유황오리 추출물의 효과가 더 높았다.

• Journal of Gastric Cancer
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• 제11권1호
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• pp.16-22
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• 2011

Purpose: Eupatilin is an antioxidative flavone and a phytopharmaceutical derived from Artemisia asiatica. It has been reported to possess anti-tumor activity in some types of cancer including gastric cancer. Eupatilin may modulate the angiogenesis pathway which is part of anti-inflammatory effect demonstrated in gastric mucosal injury models. Here we investigated the anti-tumor effects of eupatilin on gastric cancer cells and elucidated the potential underlying mechanism whereby eupatilin suppresses angiogenesis and tumor growth. Materials and Methods: The impact of eupatilin on the expression of angiogenesis pathway proteins was assessed using western blots in MKN45 cells. Using a chromatin immunoprecipitation assay, we tested whether eupatilin affects the recruitment of signal transducer and activator of transcription 3 (STAT3), aryl hydrocarbon receptor nuclear translocator (ARNT) and hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) to the human VEGF promoter. To investigate the effect of eupatilin on vasculogenesis, tube formation assays were conducted using human umbilical vein endothelial cells (HUVECs). The effect of eupatilin on tumor suppression in mouse xenografts was assessed. Results: Eupatilin significantly reduced VEGF, ARNT and STAT3 expression prominently under hypoxic conditions. The recruitment of STAT3, ARNT and HIF-$1{\alpha}$ to the VEGF promoter was inhibited by eupatilin treatment. HUVECs produced much foreshortened and severely broken tubes with eupatilin treatment. In addition, eupatilin effectively reduced tumor growth in a mouse xenograft model. Conclusions: Our results indicate that eupatilin inhibits angiogenesis in gastric cancer cells by blocking STAT3 and VEGF expression, suggesting its therapeutic potential in the treatment of gastric cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3203-3207
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• 2012

Backgrounds: To investigate the inhibiting effects of multi-target anti-microRNA antisense oligonucleotide (MTg-AMOs) on proliferation and migration of human gastric cancer cells. Methods: Single anti-microRNA antisense oligonucleotides (AMOs) and MTg-AMOs for miR-221, 21, and 106a were designed and transfected into SGC7901, a gastric cancer cell line, to target the activity of these miRNAs. Their expression was analyzed using stem-loop RT-PCR and effects of MTg-AMOs on human gastric cancer cells were determined using the following two assay methods: CCK8 for cell proliferation and transwells for migration. Results: In the CCK-8 cell proliferation assay, $0.6{\mu}mol/L$ was selected as the preferred concentration of MTg-AMOs and incubation time was 72 hours. Under these experimental conditions, MTg-AMOs demonstrated better suppression of the expression of miR-221, miR-106a, miR-21 in gastric cancer cells than that of single AMOs (P = 0.014, 0.024; 0.038, respectively). Migration activity was also clearly decreased as compared to those in randomized and blank control groups ($28{\pm}4$ Vs $54{\pm}3$, P <0.01; $28{\pm}4$ Vs $59{\pm}4$, P < 0.01). Conclusions: MTg-AMOs can specifically inhibit the expression of multiple miRNAs, and effectively antagonize proliferation and migration of gastric cancer cells promoted by oncomirs.

• Nutrition Research and Practice
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• 제4권3호
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• pp.177-182
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• 2010

The Chaga mushroom (Inonotus obliquus) has been used in folk medicine to treat cancers. However, limited information exists on the underlying anticancer effects of the major component of I. obliquus in vivo. We hypothesize that the pure compounds ($3{\beta}$-hydroxy-lanosta-8,24-dien-21-al, inotodiol and lanosterol, respectively) separated from I. obliquus would inhibit tumor growth in Balbc/c mice bearing Sarcoma-180 cells (S-180) in vivo and growth of human carcinoma cells in vitro. To test this hypothesis, the growth inhibition of each subfraction isolated from I. obliquus on human carcinoma cell lines (lung carcinoma A-549 cells, stomach adenocarcinoma AGS cells, breast adenocarcinoma MCF-7 cells, and cervical adenocarcinoma HeLa cells) was tested in vitro. Then, after S-180 implantation, the mice were fed a normal chow supplemented with 0, 0.1 or 0.2 mg of subfraction 1, 2 or 3 per mouse per day. All of the subfractions isolated from I. obliquus showed significant cytotoxic activity against the selected cancer cell lines in vitro. Subfraction 1 was more active than subfraction 2 and subfraction 3 against the A549, AGS and MCF-7 cancer cell lines in vitro. In in vivo results, subfraction 1 isolated from I. obliquus at concentrations of 0.1 and 0.2 mg/mouse per day significantly decreased tumor volume by 23.96% and 33.71%, respectively, as compared with the control. Subfractions 2 and 3 also significantly inhibited tumor growth in mice bearing S-180 as compared with the control mouse tumor. Subfraction 1 isolated from I. obliquus showed greater inhibition of tumor growth than subfractions 2 and 3, which agrees well with the in vitro results. The results suggest that I. obliquus and its compounds in these subfractions isolated from I. obliquus could be used as natural anticancer ingredients in the food and/or pharmaceutical industry.

• 한국의학물리학회지:의학물리
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• 제8권2호
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• pp.103-109
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• 1997

위암뇨에서 7 ppm과 8 ppm 사이에 네 개의 특성 핵자기공명신호가 나타남을 최근의 핵자기공명분석으로 알게 되었고 , 이 신호들은 정상뇨와 타질병 환자뇨에 비하여 자주 일어남을 발견하였다. 이 네 개의 신호들은 각각 7.25 ppm, 7.38 ppm, 7.63 ppm 그리 고 7.80 ppm이었다. 스핀결합상수의 계산에 의하면, 이 네 개의 공명신호는 종래에 알려졌던 p-hydroxyphenyl이 아니라 m-hydroxyphenyl 임이 밝혀졌다. 본 연구에서는 핵자기공명신호가 자주 일어남을 이용하여 암진단을 가능케 하였고, 이들 네 방향족 핵자기공명신호의 체적자화율을 측정하여 타 목적에 기여할 수 있게 하였다. 체적자화율의 측정 결과는 각각 10.01$\times$$10^{-6}$, 10.07$\times$$10^{-6}$, 10.19$\times$$10^{-6}$ 그리고 10.27$\times$$10^{-6}$이었고, m-hydroxyphenyl의 총체적자화율은 10.27$\times$$10^{-6}$이었다.

• Biomolecules & Therapeutics
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• 제24권5호
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• pp.501-509
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• 2016

Shikonin, which derives from Lithospermum erythrorhizon, has been traditionally used against a variety of diseases, including cancer, in Eastern Asia. Here we determined that shikonin inhibits proliferation of gastric cancer cells by inducing apoptosis. Shikonin's biological activity was validated by observing cell viability, caspase 3 activity, reactive oxygen species (ROS) generation, and apoptotic marker expressions in AGS stomach cancer cells. The concentration range of shikonin was 35-250 nM with the incubation time of 6 h. Protein levels of Nrf2 and p53 were evaluated by western blotting and confirmed by real-time PCR. Our results revealed that shikonin induced the generation of ROS as well as caspase 3-dependent apoptosis. c-Jun-N-terminal kinases (JNK) activity was significantly elevated in shikonin-treated cells, thereby linking JNK to apoptosis. Furthermore, our results revealed that shikonin induced p53 expression but repressed Nrf2 expression. Moreover, our results suggested that there may be a co-regulation between p53 and Nrf2, in which transfection with siNrf2 induced the p53 expression. We demonstrated for the first time that shikonin activated cell apoptosis in AGS cells via caspase 3- and JNK-dependent pathways, as well as through the p53-Nrf2 mediated signal pathway. Our study validates in partly the contribution of shikonin as a new therapeutic approaches/agent for cancer chemotherapy.

• 분석과학
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• 제28권6호
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• pp.417-424
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• 2015

본 연구에서는 한국인을 대상으로 하여 혈중 셀레노단백질의 함량과 위암과의 상관관계를 연구하였다. 혈청 속에서의 셀레노단백질의 함량을 종별로 분석하여, 각 단백질의 함량과 위암과의 상관관계를 알아보고 셀레늄의 위암에 대한 생체지표로서의 가능성을 알아보고자 하였다. SPE를 사용하여 방해 이온들을 제거한 뒤 후컬럼 동위원소법으로 정량한 기존의 복잡한 방법과 달리 SPE를 사용하지 않고 m/z 78을 모니터링하고 검정곡선법으로 정량하였다. 표준인증물질인 CRM BCR-637을 사용하여 정확도를 검증한 결과 72.20±3.35 ng·g⁻¹로서 기준값인 81±7 ng·g⁻¹에 만족할 만한 결과를 얻었다. 먼저 통제군인 순환기 질환 환자 혈청 속의 셀레노단백질의 총농도를 조사해본 결과 105.70±21.20 ng·g⁻¹으로서 정상인의 값과 차이가 없었다. 각 종별 농도를 보면 GPx와 SelP, 그리고 SeAlb이 각 각 26.12±7.84, 65.15±14.50, 14.43±6.99 ng·g⁻¹이며 분포는 24.7%, 61.6%, 및 13.7% 이었다. 한국인의 위암 환자의 혈청을 분석한 결과, GPx와 SelP, 그리고 SeAlb의 농도는 각각 15.41±9.01, 50.83±17.9, 그리고 9.87±5.21 ng·g⁻¹이며 총 농도는 76.11±28.12 ng·g⁻¹으로 대조군에 비하여 총량과 각 종들도 모두 큰 감소를 보였다. 감소폭은 GPx는 41.0%, SelP 22.0%, SeAlb 31.6% 이었다. 하지만 종별 분포는 유의미한 차이를 보이지 않았고 따라서 위암 환자의 경우 셀레노단백의 총량을 분석하거나 또는 그 중 하나의 단백질을 사용하여도 암을 진단하는 보조적인 지표가 될 수 있을 것으로 생각되었다. 또한 나이별에 따른 분석을 보면 노년인 50대 부터 70대에서는 실험군과 대조군이 확실한 차이를 보이나 30대와 40대에서는 별 차이를 보이지 않고 있으므로 성별, 나이별에 따른 조사가 더 필요할 것으로 보인다.

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2004년도 The 13th Korea Genome Conference
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• pp.34.2-34.2
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• 2004

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10483-10487
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• 2015

Heptaphylline derivatives are carbazoles in Clausena harmandiana, a medicinal plant that is utilized for headache, stomach ache, and other treatments of illness. The present study examined the effects of heptaphylline and 7-methoxyheptaphylline on apoptosis of human colon adenocarcinoma cells (HT-29 cell line). Quantification of cell viability was performed using cell proliferation assay (MTT assay) and of protein expression through immunoblotting. The results showed that only heptaphylline, but not 7-methoxyheptaphylline, significantly significantly activated cleaved of caspase-3 and poly (ADP-ribose) polymerase (PARP-1) which resulted in HT-29 cell death. We found that heptaphylline activated BH3 interacting-domain death agonist (Bid) and Bak, proapoptotic proteins. In contrast, it suppressed X-linked inhibitor-of-apoptosis protein (XIAP), Bcl-xL and survivin, inhibitors of apoptosis. In addition, heptaphylline inhibited activation of NF-${\kappa}B$/p65 (rel), a regulator of apoptotic regulating proteins by suppressing the activation of Akt and $IKK{\alpha}$, upstream regulators of p65. The findings suggested that heptaphylline induces apoptosis in human colon adenocarcinoma cells.