• Journal of Acupuncture Research
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• v.18 no.4
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• pp.152-160
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• 2001

Objective : This study was undertaken to determine if Plantaginis Semen Aquacupuncture(PSA) has a protective effect against $CCl_4$ induced Hepatoxicity in Rats. Methods : The experimental group were divided into Normal group(untreated group), Control group, PSA group. Rats were administered orally $CCl_4$(0.1 ml/kg) for 4 days. In experiments for PSA effect, rats received 0.1 ml of PSA extraction in both sides of corresponding $G{\bar{a}}nsh{\bar{u}}$(BL18) of human body for 3 days after treated $CCl_4$. Variation of weight and biochemical assays(GOT, GPT, LDH, ALP, total cholesterol, triglyceride, albumin) were performed. Results : In Control group, $CCl_4$ increased serum GOT, GPT, LDH, ALP and albumin and decreased weight, total cholesterol and triglyceride. PSA significantly decreased serum GOT, GPT, LDH and increased total cholesterol as compared with Control group. Conclusion : These results indicate that PSA could be used in prevention and treatment of hepatoxicity. However, precise mechanisms of PSA protection remain to be determined.

• Membrane Journal
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• v.23 no.4
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• pp.297-303
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• 2013

Diagnostic membranes which were made of polyphosphazenes were prepared for the measurements of blood glucose concentration. The effects of possible interferents in human blood on the glucose concentration measurements were examined. Above those interferents, ascorbic acid (AA) showed 3~9% higher K/S values compared to the standard plasma solution, tolbutamide (TA) showed 11~13% lower K/S values than those of plasma, triglycerides and bovine serum albumin (BSA) showed 20~25% lower K/S values than those of plasma. However, most of the interferents except above materials did not show any serious effects on the blood glucose measurements.

• BMB Reports
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• v.38 no.5
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• pp.577-583
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• 2005

Subtilisin QK, which is newly identified as a fibrinolytic enzyme from Bacillus subtilis QK02, has the ability of preventing nitrotyrosine formation in bovine serum albumin induced by nitrite, hydrogen peroxide and hemoglobin in vitro verified by ELISA, Western-blot and spectrophotometer assay. Subtilisin QK also attenuates the fluorescence emission spectra of bovine serum albumin in the course of oxidation caused by nitrite, hydrogen peroxide and hemoglobin. Furthermore, subtilisin QK could suppress the transformation of oxy-hemoglobin to met-hemoglobin caused by sodium nitrite, but not the heat-treated subtilisn QK. Compared with some other fibrinolytic enzymes and inactivated subtilisin QK treated by phenylmethylsulfonylfluoride, the ability of inhibiting met-hemoglobin formation of subtilisin QK reveals that the anti-oxidative ability of subtilisin QK is not concerned with its fibrinolytic function. Additionally, nitrotyrosine formation in proteins from brain, heart, liver, kidney, and muscle of mice that is intramuscular injected the mixture of nitrite, hydrogen peroxide and hemoglobin is attenuated by subtilisin QK. Subtilisin QK can also protect Human umbilical vein endothelial cell (ECV-304) from the damage caused by nitrite and hydrogen peroxide.

• Proceedings of the KAIS Fall Conference
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• 2000.10a
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• pp.243-244
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• 2000

Serum albumin은 혈청 단백질 중 50-60%를 차지한다. 알부민은 순환계 내에서 삼투압을 유지시켜 세포내외의 체액을 유지 및 지용성 물질치 이동에 관여한다. 신사구체와 기저막의 투과성이 증가하거나 세뇨관의 흡수감소 등으로 단백질의 요배설량이 하루에 150mmg 이상되는 단백뇨는 신장내의 병변이 있음을 나타내는 중요한 지표이다. 이러한 단백뇨는 크게 사구체성 단백뇨, 세뇨관성 단백뇨, 혼합성 단백뇨로 나눌수 있는데 이중 사구체 기저막의 손상으로 발생되는 사구체성 단백뇨는 알부민과 같은 고분자량의 단백질이 소변 내로 배설되는 현상이다. 일반적으로 알부민이 정상치 이상으로 소변을 통하여 배설되는 것을 'microalbuminuria'라고 하며 당뇨병으로 인한 신장병변을 예견하는데 중요한 지표로 알려져 있다. Microalbuminuria는 방사 면역확산법, 방사성 면역측정법, 면역혼탁측정법, 비탁측정법, 효소결합면역측정법(ELISA) 등으로 검출할 수 있으나 최근에는 안전하면서도 민감도가 높고 측정이 용이한 방법인 ELISA를 이용한 면역분석방법이 많이 사용되고 있다. 면역특이성이 떨어지는 다세포군 항체를 사용한 방법의 문제점을 극복하기 위해서 세포융합기술을 이용한 단세포군항체를 생산하는 세포주를 개발하였고 그 특성을 규명하였다. 따라서 본 연구에서 세포융합기술을 도입하여 개발된 인간 혈청 알부민에 대한 단세포군항체는 glycohemoglobin에 대한 단세포군항체와 더불어 당뇨병의 진행 정도를 진단할 수 있는 키트의 원료로 사용될 수 있다.

• Proceedings of the KOR-BRONCHOESO Conference
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• 2010.03a
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• pp.43-43
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• 2010

• Journal of Korean Neurosurgical Society
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• v.30 no.1
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• pp.12-19
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• 2001

Objective : Albumin is a very useful drug for the improving of cerebral blood volume and the oncotic effect in cerebral ischemia or cerebral vasospasm. The purpose of this study was to examine the morphological and neurological effect of albumin therapy on reperfusion injury following transient focal cerebral ischemia. Materials and Methods : 18 Male Sprague-Dawley rats weighing 270-320g were used. The ischemia model was produced by 2-hour period of transient middle cerebral artery occlusion with a poly-L-lysin coated intraluminal suture. The agent(20% human serum albumin[HSA]) or control solution(NaCl 0.9%) was administered intravenously at a dosage of 1% of body weight immediate after reperfusion following a 2-hour period occlusion. Neurological function was evaluated by the postural reflex and the forlimb placing test during occlusion(at 60 min) and daily for 3 days thereafter. The brain was perfusion-fixed, and infarct volumes and brain edema were measured. Results : The HSA significantly improved the neurological score in treated group. The rats of albumin treatment group showed significantly reduced total infarct volume(by 34%) and brain edema(by 81%) compared with salinetreated rats. Conclusion : HSA showed a substantial effect on the transient focal cerebral ischemia and reperfusion injury model. These results may indicate its usefulness in treating reperfusion injury patients after thrombolysis treatment for the thrombo-embolic major cerebral artery occlusions.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.1
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• pp.9-16
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• 1998

This study was performed to investigate the stimulating effect on oocyte maturation and cumulus cell expansion in TC199 media by human cord serum (HCS) supplementation. Immature mouse oocyte cumulus complexes (OCCs) were cultured in TC199 media supplemented with bovine serum albumin (BSA), HCS and human chorionic gonadotropin (hCG) instead of luteinizing hormone (LH) respectively, and the expression of cumulus expansion and oocyte maturation were observed. After 4hr and 24hr culture with or without OCCs, media containing 0.4% BSA, 10% HCS and 10 IV hCG respectively were collected and analyzed for changing concentrations of estradiol $(E_2)$, progesterone $(P_4)$, testosterone (T), and $PGF_{2\alpha}$. There were no elevation of $E_2$, T, and $PGF_{2\alpha}$ by OCCs culture, but minute elevation of $P_4$ level by 24hr OCCs culture in hCG supplementation (p=0.048). The stimulating pattern of cumulus expansion of OCCs by HCS and hCG supplementation was similar to our previously report using Ham's F-10 media, however oocyte maturation rates after 24hr OCCs culture in all media were increased by $20\sim30%$ compared to Ham's F-10 media. These results suggest that LH in HCS induce cumulus expansion probably by $P_4$ secretion of OCCs, and TC199 is efficient media for immature mouse oocyte maturation.

• Journal of Pharmaceutical Investigation
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• v.31 no.2
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• pp.73-80
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• 2001

Lung perfusion scanning, invariably combined with ventilation studies provides a reliable and non-invasive mean to diagnose lung related pathologies despite the availability of modern techniques such as angiography, magnetic resonance imaging, magnetic resonance angiography, and helical (spiral) computed tomography. The technique involves the generation of images by radiations emitted from radioisotopes introduced in to the lungs. Various radiopharmaceuticals have been proposed and designed to incorporate $Tc^{99m}$ in to macroparticulate form for lung perfusion imaging. However, most of these have associated difficulties such as reproducibility of the product with regards to particle size distribution and poor elimination from the lung capillary bed. $Tc^{99m}$ macroaggregated albumin $(Tc^{99m}-MAA)$ is used extensively for clinical lung perfusion imaging and is considered as the radiopharmaceutical of choice. It is non-toxic, safe, and being biodegradable, is easily eliminated from the lung capillary bed by proteolytic enzyme metabolism and by mechanical forces due to lung movement.

• Journal of the Korean Chemical Society
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• v.4 no.1
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• pp.1-6
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• 1957

The mobilities and the electrophoretic phenomena of human serum albumin, and of horse serum albumin, etc., on paper were reported, but there is no information about the mobility and the electrophoretic phenomena of ovalbumin. In this study they are determined and considered at various time durations. The mean value of this mobility is compared with the value determined by the electrophoresis in solution. Also, the mobilities of the electroendosmotic flow, which affect on the mobility seriously, are determined at various time durations. Apparatus and method used, are a closed system type and a method with electrode vessels. It is concluded that: 1) The mobilities of ovalbumin have the nearly constant mobilities at six and eight hours of time durations, but it decreases at ten hours of long time duration. Their mean value is (0.243 ${\pm}$ 0.003)cm./hr./v./cm. toward anode at pH 8.0. 2) Under these experimental conditions, ovalbumin cannot be separated into three fractions (A1, A2, A3) on paper. 3) Most of the factors, which affect on the mobility, are fixed by the initial experimental conditions, but the electroendosmotic flow due to the characteristic capillarity of paper is measured. Then, the mean value of its mobilities shows the good constancy, and the value is (0.073 ${\pm}$ 0.0003)cm/hr./v./cm. toward cathode at pH 8.0. 4) By the above facts, if the same paper and the same experimental conditions are chosen, it may be preferred to determine the mobility of the electroendosmotic flow once time, even when many observations are required.

• Korean Journal of Acupuncture
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• v.23 no.2
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• pp.155-166
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• 2006

Objectives: This study was undertaken to determine if Plantaginis Semen(PS) has a protective effect against $CCI_4$ induced hepatoxicity in Rats. Methods: The experimental groups were divided into Normal group (untreated Group), Control group $(group\;treated\;with\;CCI_4)$, Sample 1 (group treated with 0.1 ml Plantaginis Semen herbal acupuncture in both sides of $G\bar{a}nsh\bar{u}\;(BL_{18})$ after $CCI_4$ treating) and Sample 2 (group treated with feeding of 1 cc Plantaginis Semen extraction after $CCI_4$ treating). Rats were administered orally $CCI_4\;(0.1\;ml/kg)$ for 4 days. In experiments for PS effect, rats of Sample 1 group received 0.lml herbal acupuncture of PS extraction in both sides of corresponding $G\bar{a}nsh\bar{u}\;(BL_{18})$ of human body for 3 days after treated $CCI_4$. Rats of Sample 2 group feeded PS extraction (1cc) for 3 days after treated $CCI_4$. Variation of weight and biochemical assays (AST, ALT, LDH, ALP, total cholesterol, triglyceride, total protein, albumin) were performed. Results: In Control group, $CCI_4$ increased serum AST, ALT, LDH, ALP, total protein, albumin and decreased weight, total cholesterol, triglyceride. Sample 1 and Sample 2 significantly decreased serum AST, ALT, LDH, ALP, total protein, albumin as compared with Control group. Conclusion: These results indicate that PS could be used in prevention and treatment of hepatoxicity. However, precise mechanisms of PS protection remain to be determined.