• Korean Chemical Engineering Research
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• v.44 no.1
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• pp.58-64
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• 2006

We successfully isolated human anagen hair follicles from human scalp skin by microdissection and tried to culture them under various conditions. First we confirmed negative effect of serum on human hair follicle organ culture. As a next step serum-free medium compositions, Philpott medium, IMDM, and DHGM (Dongguk hair growth medium) were tried. Philpott medium is a general medium for hair organ culture based on Williams' E medium and DHGM is a special self-developed medium containing high amino acids and vitamins (B group) composition. As results, hair follicle in Philpott medium and IMDM showed anagen phase morphological structure, but rapid loss of hair elongation, low alkaline phosphatase expression, and very low expression of CK19. It is thought these hair follicles rapidly regressed from apoptosis. However, hair follicles in DHGM showed long term anagen phase morphological structure, continuous hair elongation, high alkaline phosphatase, and CK19 expression. These results demonstrate that high amino acids and vitamins (B group) composition are essential to in vitro long term human hair follicle organ culture and this culture medium will be useful in basic study of hair biology or application study to the development of alopecia treatment drugs.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.228-228
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• 2005

• Journal of the Korean Society of Clothing and Textiles
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• v.41 no.3
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• pp.556-574
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• 2017

This study explored the appearance management experiences of breast cancer patients undergoing mastectomy. For data collection, one-on-one interviews were held with 18 Korean females diagnosed with breast cancer from the first stage to the third stage from 2008 to 2015. The data was analyzed through a three-step analysis of grounded theory. QSR NVivo 11.0 for Mac was applied in order to avoid biased results according to the subjective viewpoint of researchers. From open coding results, six paradigms and 15 categories were drawn. The results of axial coding showed that the psychological changes category was the central phenomenon. Additionally, three categories (skin changes, scalp/hair changes, and general reactions) were found to be causal conditions, and five paradigms (clothing behavior, skin care behavior, makeup behavior, scalp/hair-care behavior, and shopping behavior) were found to be action/interaction strategies. Last, selective coding integrated fragmented qualitative data through open coding and axial coding in order to derive explanatory narratives. A comprehensive examination of the appearance management behaviors of breast cancer patients suffering from extreme stress due to physical/psychological changes enables this study to provide a foundation for related studies to be activated in the clothing and textiles discipline.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.359-362
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• 2003

The recent development of methods for culturing hair follicles in vitro has proved an important tool to investigate many aspects of drug screening. Human hair follicle is composed of multiple types of cells, whose interactions regulate morphology and cycling-anagen, catagen, and telogen. Many investigators have tried to develop models to prolong of the period of hair elongation in vitro. However these are limited in submerged culture, which don't work due to the lack of cell-cell interactions which are abundant in vivo environment. So we applied dermal equivalent (DE) to culturing flair follicles to prolong hair growth period.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.365-368
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• 2003

Hair follicles develop as a result of epithelial-mesenchymal interactions between epidermal keratinocytes and dermal cells. Moreover hair follicles constitute multiple cells that influence hair follicle development and cyclic activity. We isolated some cells using explantation and enzymatic digestion method from human scalp hair follicles. So we could culture some follicular cells, such as outer root sheath (ORS) cells, dermal papilla (DP) cells, dermal sheath (DS) cells, matrix cells and melanocyte.

• Journal of the Korea Convergence Society
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• v.13 no.5
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• pp.35-43
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• 2022

It was to test the efficiency of essence containing scalp derived Leuconostoc mesenteroides extract on relieving hair loss and the possibility of the usage as the functional cosmetic's material. 41 subjects were classified as the control and test group and for 24 weeks, from Apr. 15th 2021 to Nov. 4th 2021, they were double blindness, contrast samples, single organs, and randomized human applicating tests were performed. The amount of hair had meaningful change (p<0.001) seen through phototricogram in 8,16,24 weeks each, and could tell the difference by researcher's bare eye after 24 weeks. Since there were meaningful changes (p<0.017) in subjective evaluation of the subject on after 8,16,24 weeks time, Essence which contains 1% of L. mesenteroides has efficiency on hair loss. Therefore, L. mesenteroides is expected to be used as various cosmetics to prevent hair loss.

• BMB Reports
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• v.46 no.9
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• pp.460-464
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• 2013

The progression of androgenetic alopecia is closely related to androgen-inducible transforming growth factor (TGF)-${\beta}1$ secretion by hair follicle dermal papilla cells (DPCs) in bald scalp. Physiological levels of androgen exposure were reported to increase reactive oxygen species (ROS) generation. In this study, rat vibrissae dermal papilla cells (DP-6) transfected with androgen receptor showed increased ROS production following androgen treatment. We confirmed that TGF-${\beta}1$ secretion is increased by androgen treatment in DP-6, whereas androgen-inducible TGF-${\beta}1$ was significantly suppressed by the ROSscavenger, N-acetyl cysteine. Therefore, we suggest that induction of TGF-${\beta}1$ by androgen is mediated by ROS in hair follicle DPCs.

• Korean Journal of Human Ecology
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• v.17 no.1
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• pp.105-114
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• 2008

This study aims on observing the social recognition and its aspects for men taking care of their outer appearances at current point where men are rapidly rising as the main consumer in the beauty industry while their interest for outer appearances are increasing. Therefore, we observed the overall trend and changes in social recognition for male looks by analyzing the contents of articles in fashion magazines that play the critical role of spreading the trend while suggesting and delivering the diverse trend changes to the public to correspond to the individual and concrete demands of certain class segmented into gender, age, hobby, and more. As a result, articles related to outer appearances were divided into fashion, cosmetic and skin, hair and scalp, body figure care, cosmetic surgery, and others, and among them, fashion category took up the highest ratio. Also, articles related to outer appearances increased even more according to the change in time, where articles related to fashion and hair increased while articles of other categories decreased.

• Journal of Life Science
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• v.29 no.7
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• pp.779-784
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• 2019

Free radicals are known to inhibit hair vitality by damaging the cell membranes of the hair follicles. The purpose of this study was to determine the antioxidant activities and the capacity for hair loss prevention of extracts from Platycodon grandiflorum. We prepared butanol (BF) and water (WF) fractions from P. grandiflorum. DPPH and ABTS radical scavenging activities were measured to investigate the antioxidant activities of the fractions. Both fractions exhibited dose-dependent antioxidant activities for DPPH radical production, and BF and WF almost completely suppressed ABTS radical production when supplied at 10 and 100 mg/ml, respectively. We confirmed a skin regeneration effect by treating human HaCaT skin cells with a range of BF and WF concentrations for 24 and 48 hr. The extract treatments accelerated cell proliferation. We also assayed the capacity of BF and WF to suppress inflammation using RAW264.7 cells. BF dose-dependently suppressed nitrous oxide (NO) production. Treatment of human hair follicle dermal papilla cells (HFDPC) with BF and WF promoted cell proliferation after 24, 48, and 72 hr of treatment when supplied at 10, 50, 100, and $200{\mu}g/ml$. Taken together, these results confirm the possibility of using BF and WF extracts from P. grandiflorum in formulating hair loss prevention products.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.179-182
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• 2017

Cerebral nervous system intervention procedures have been reported frequently due to radiation exposure such as skin baldness, hair loss, and redness due to prolonged procedures. Therefore, the bismuth shield designed to reduce the radiation exposure of the target organ located in the anterior part of the human body sensitive to skin and radiation sensitivity during CT (computed tomography) scan is applied to the cerebral vascular system intervention by ergonomic design, To reduce the radiation dose of sensitive scalp, we propose a study.