• Biomolecules & Therapeutics
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• v.6 no.3
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• pp.276-282
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• 1998

Retinoids regulate a wide variety of biological processes such as cellular proliferation and differentiation in many cell types. They have also shown to stimulate replication of several viruses including human cytomegalovirus (CMV). Retinoid signalling pathway involves two distinct subfamilies of nuclear receptors, retinoic acid receptors (RARs) and retinoid X receptors (RXRs) that bind to specific retinoic acid response elements (RAREs) in the promoter regions of retinoid-target genes. Here, we characterized RAREs in the regulatory regions of the CMV and of the hepatitis B vi.us (HBV). The viral RAREs, i.e., CMV-RARE and HBV-RARE, are composed of two consensus RARE half-sites (A/GGGTCA) arranged as a direct repeat separated by 5-bp and 1-bp, respectively. The RAREs were activated by both RAR/RXR heterodimers and RXR homodimers in transient transfection experiments. We also found that COUP-TF$\alpha$ (chicken ovalbumin upstream promoter-transcription factor u) and COUP-TF$\beta$ repressed the retinoid response of the viral elements. Further we demonstrated that previously known retinoid antagonist, SRI 1330, repressed retinoid-induced transactivation of the CMV-RARE. These results implicate Vitamin A, it's nuclear receptors and COUP-TFs as important regulators of the CMV and HBV pathogenesis and the SRl1330 as potential negative modulator of such retinoid-dependent processes.

• Parasites, Hosts and Diseases
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• v.39 no.1
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• pp.43-48
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• 2001

We have studied the genetic differences among four isolates of Trichinella including a new strain of Trichinella spiralis (ISS 623) recently found from a human case who took a badger in Korea. Because they have a different host origin and came from geographically separated regions, we supposed the genetic pattern of the isolates might be different as had been previously reported. It was analysed by PCR-RFLP analysis of the rDNA repeat that can readily distinguish a species or strain from others. Isolated genomic DNA of each isolate of Trichinella larvae was amplified with ITSl specific primers and digested with restriction endonucleases. The PCR product of ITSl was confirmed using Southern blot analysis to be a 910 Up fragment. The restriction fragments of each isolate had variable patterns when it was digested with Rsa I only. According to the RFLP patterns, the estimated genetic divergence between each isolate was different. In conclusion, four isolates of Thichinella including a new strain of T. spiralis obtained from a Korean patient may have genetic differences in the ITSl region and the Shanghai isolate was genetically more similar to the Japanese unknown isolate than others in the ITSl region.

• Journal of Environmental Science International
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• v.14 no.8
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• pp.739-748
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• 2005

The purpose of this study is to analyze seasonal changes in structure and landscape of Gongji stream corridor in Chuncheon, and to suggest some guidelines to contribute to creating a desirable close-to- nature stream. The study seasonally surveyed floodplain and revetment conditions, channel micro-topography, streamflow level and velocity, and vegetational cover. Flooding, water level, and vegetation were major factors of affecting seasonal changes in streambed structure and stream landscape. Small sand bars and islands were considerably disturbed by flooding and water level change. However, large islands and sand bars in the upper and middle section of the study stream remained or reappeared even after flooding. Flooding also tended to repeat channel sedimentation at the same spot. Controlling water volume of the Euiam Lake, which is adjacent to the study stream, caused higher water level downstream in the dry seasons. The majority of vegetation in sand bars and islands was washed away by the floods. Vehicle passing, crop cultivation, and ball game were other elements which disturbed vegetation in the floodplain. Creating a close-to-nature stream should reflect micro-topographical changes of channel by flooding, prevent improper vehicle entry and human use, and remove concrete material in the revetment and floodplain.

• The Research Journal of the Costume Culture
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• v.14 no.6
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• pp.1042-1055
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• 2006

The purpose of this study is to consider the formative characteristics of dance sports from such points of view as shapes, colors, material and decorations, and to examine artistic characteristics that are expressed through those elements. As a result, this study shows five artistic characteristics as follows; First, rhythmic characteristic is expressed with repeat of lines that is formed by pleats, shirring or gather, with duplication of ruffle's wave, with gradation of colors, with Moire-effect that is generated with overlap of see-through material, with glossy material that reflects lights to a great extent, with fringe. Second, bodily characteristic emphasizes the beauty of human bodies through direct or indirect body exposure by way of cut-and-open, see-throughness or close adhesion. Third, decorative characteristic is shown with beads, fringe, ruffle, embroidery, shirring, binding, or feather. Fourth, the beauty of female body is manifested to express eroticism, which is culminated by body exposure, body consciousness, use of illuminating or see-through material or colorful trimming. Finally, elegant characteristic is revealed through feminity, sophistication, and harmony that are expressed with black and white colors, see-through material, or bead trimmings.

• Journal of the Korean Society of Safety
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• v.28 no.1
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• pp.137-143
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• 2013

Cultural heritage passing down to posterity is a global concern. But there are many culture heritage that not remained to now disappeared by various disasters. It is told that disaster is learnt from disaster. The most fundamental task in disaster prevention is to collect and analyze cases of accidents so as not to repeat a disaster. In other words, an analysis of various disaster-related materials and their countermeasures in the Joseon period is an actual condition-based phenomenological methodology used to prevent disasters at cultural properties today. In this context, it is important to collect histories of disasters in the Joseon period, to extract disaster-related historical data and to categorize the data to build a disaster literature database. This study has produced [Database of Disasters in the Joseon Era], a collection of articles on fire outbreaks based on The Annals and The Daily Records belonging to the Memory of the World. On the basis of this chronology, human and property damages caused by fires in Joseon have been examined, identified by districts, as well as disaster technologies, have been analyzed. It has also built disaster vulnerability maps in the Joseon Dynasty Era.

• Tunnel and Underground Space
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• v.20 no.2
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• pp.112-118
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• 2010

Before blasts that can have direct impacts on human bodies or structures, it is necessary to assess impacts of ground vibration. Therefore, frequency has been recognized as an important factor in order to assess impact on ground vibration and damages. There have been many studies on impacts of frequency. But, there have been no studies on relations between vibration and frequency according to delay time difference. In this study, we examined the relations between delay time difference and frequency according to each frequency with which reinforcement and destructive intervention repeat through delay time difference obtained using superposition modeling of single hole blasting waveform based on the theory of time difference developed by Langefors.

• Journal of Genetic Medicine
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• v.3 no.1
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• pp.21-24
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• 1999

To study the X chromesome mosaicism in the cytogenetically pure 45,X Turner syndrome patients, we applied PCR technique using DNAs extracted from archived cytogenetic slides. We amplified the DNAs using nested primers targeted to a highly polymorphic short tandem repeat(STR) of the human androgen receptor gene(HUMARA) for the detection of X chromosome mosaicism. This assay is a very sensitive and useful method which can be applied to the DNAs extracted from archived cytogenetic slides to detect X mosaicism. We have tested 50 normal Korean females to determine whether the HUMARA locus is highly polymorphic among Koreans. 85% of Korean population showed heterozygosity in the HUMARA locus. We analysed the 24 DNAs extracted from archived slides of patients and abortuses with Turner syndrome in cytogenetic analysis. We observed the heterozygosities of 50% from pure 45,X patients, 83% from the patients with mosaic Turner syndrome and 8.3% from the abortuses of pure 45,X. Using the PCR technique of the HUMARA locus in the archived cytogenetic slides, we detected X chromosome mosaicism which could not be detected in cytogenetic analysis.

• Microbiology and Biotechnology Letters
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• v.49 no.4
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• pp.594-602
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• 2021

The NLRP3 (nucleotide-binding domain, leucine-rich repeat family pyrin domain containing 3) inflammasome plays an important role in the initiation of inflammatory responses, through the recognition of pathogen-associated molecular patterns and tumor progression, including tumor growth and metastasis. In this study, we examined the effects of defective NLRP3 on the growth, migration, and invasiveness of hepatocellular carcinoma (HCC) SK-Hep1 cell. First, HCC SK-Hep1 cells were transfected with human NLRP3 targeting LentiCRISPRv2 vector using the CRISPR-Cas9 system, and NLRP3 deficiency was confirmed by RT-qPCR and western blotting. NLRP3 deficient SK-Hep1 cells showed delayed cell growth and decreased protein expression of PI3K, p-AKT, and pNF-κB when compared to NLRP3 complete SK-Hep1 cells. In addition, NLRP3 deficiency arrested the cell cycle at G1 phase through an increase in p21 and a reduction in CDK6. NLRP3 deficient SK-Hep1 cells also showed significantly delayed cell migration, invasion, and wound healing. The expression of epithelial-mesenchymal transition signaling molecules, such as N-cadherin and MMP-9, was found to be dramatically decreased in NLRP3 deficient SK-Hep1 cells compared to NLRP3 complete SK-Hep1 cells.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.55-55
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• 2022

Corylopsis Siebold & Zucc. (Hamamelidaceae) is widely used for horticultural plant and comprise ca. 25 species in East Asia (1 species in Korea; 4 species in Japan; 20 species in China). Previous revisions have gone from 7 to more than 30 species, causing confusion in the nursery industry and public gardens. Due to morphological similarity within Corylopsis, molecular research is needed to distinguish it. In this study, the chloroplast genome of C. gotoana and C. pauciflora distributed in Japan was completed by using NGS (Next-Generation Sequencing) technique. The genome size of C. gotoana and C. pauciflora were 159,434 bp (large single-copy (LSC): 88,164 bp; small single-copy (SSC): 18,702 bp; inverted repeat regions (IRs): 26,284 bp) and 159,363 bp (LSC: 88,097 bp; SSC: 18,700 bp; IRs: 26,283 bp), respectively. In addition, we investigated the repeats, SNPs, and indels, and that could be used as DNA markers. Phylogenetic analysis demonstrated that C. pauciflora was sister to C. gotoana and C. spicata. The genus Corylopsis is a monophyletic group and Loropetalum is closely related to Corylopsis. The results of our study will provide the basic data necessary for the analysis of the species identification markers and genetic diversity within the genus Corylopsis in the future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.102-110
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• 2019

Today's professional sports teams are in a highly competitive environment with no prospects in sight. A that was considered the favorite to win a championship can suffer from a severe slump, a team that was expected to be at the bottom can achieve victory. This unexpected situation is continuing to repeat. It is also a sign of the need to look at the use of human resources and strategies in the sports industry. Accordingly, this study looks at Daegu FC soccer team, Korea's first team for citizens. A case study on human resources innovation was done. This study introduces an argument for ordinary resources (AOR) from Frey et al. (2015) for a theoretical review and a case analysis of domestic professional football. This study determines the direction of how Daegu FC should proceed. The results of the research are as follows: first, the success of harmonizing strategic and mundane resources, and second, belief in coaches. The main contribution of this research is that it presents the importance of human resources, which is one of basic tactics that sports teams should utilize. In the sports industry, where the temporality is underlined for its clear beginning and ending, teams are more likely to use diverse sports tactics to obtain superior grades, which enriches the basis of their tactics.