• Journal of Microbiology and Biotechnology
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• v.33 no.5
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• pp.680-686
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• 2023

Aging is a complex series of multi-organ processes that occur in various organisms. As such, an in vivo study using an animal model of aging is necessary to define its exact mechanisms and identify anti-aging substances. Using Drosophila as an in vivo model system, we identified Crataegus pinnatifida extract (CPE) as a novel anti-aging substance. Regardless of sex, Drosophila treated with CPE showed a significantly increased lifespan compared to those without CPE. In this study, we also evaluated the involvement of CPE in aging-related biochemical pathways, including TOR, stem cell generation, and antioxidative effects, and found that the representative genes of each pathway were induced by CPE administration. CPE administration did not result in significant differences in fecundity, locomotion, feeding amount, or TAG level. These conclusions suggest that CPE is a good candidate as an anti-aging food substance capable of promoting a healthy lifespan.

• Food Science and Preservation
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• v.24 no.7
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• pp.975-982
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• 2017

Health of human intestine has deteriorated due to excessive stress and western diet. In this study, Vigna angularis was fermented by Bacillus subtilis KCCM11965P in order to develop prebiotic resource for improving bowel movement. The contents of ash and crude protein were $3.35{\pm}0.04%$ and $21.1{\pm}0.19%$ respectively. Vigna angularis extract (1, 3, 5%) were incubated with 3% (v/v) Bacillus subtilis KCCM 11965P for 0, 24, 48, and 72 h. Total bacterial numbers showed that the combination of 3% powdered seeds and 72 h incubation time was optimum condition for this experiment. Total polyphenol content increased from $0.18{\pm}0.010mg/mL$ in pre-incubation to $0.23{\pm}0.007mg/mL$ in post-incubation with the condition mentioned above. DPPH radical scavenging activity also increased from $36.1{\pm}6.0%$ to $63.6{\pm}5.2%$. Analysis of protease activity showed $2.69{\pm}0.003unit/mL$ in combination of 5% powdered seeds and 72 h incubation time. Amylase activity increased from $1.0{\pm}0.1unit/mL$ in pre-incubation to $26.0{\pm}0.2unit/mL$ in post-incubation. The analysis of free amino acids after incubation with Bacillus subtilis KCCM 11965P showed that leucine increased from 5.22 mg/L to 67.59 mg/L and tyrosine, one of non-essential amino acid also increased 10.08 mg/L to 259.35 mg/L by incubation with 5% powdered seeds. Most of organic acid were reduced by incubation for 72 h. These results suggest that Vigna angularis could be utilized most as a prebiotic resources.

• Research in Community and Public Health Nursing
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• v.9 no.1
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• pp.40-63
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• 1998

A long life is the desire of many people. The purpose of this study was to describe long life factors for people aged one hundred and above on Chju island. The research was conducted using a phenomenological method to add understanding to this subject. The field work for this study was done from July, 1997 to February, 1998 on Cheju island. The sample consisted of 10 persons of one hundred or above and 12 families. They were audio-recorded and analyzed using Colaizzi's (1978) method. The results of the present study shows the following: The thoughts of the persons one hundred and above showed ten important concepts; 'dietary regimen', 'mental regimen', 'sleep regimen', 'residence regimen', 'health regimen', 'labor regimen', 'exercise regimen', 'being born', 'filial piety and posterity', 'attachment to life'. Concerning 'Dietary Regimen': Dietary regulation, Preferential food intake, Living on vegetables, Light eating, and concern for longevity in food intake were important. Concerning 'Mental Regimen' : Harmony, Clearheadedness, Mildness, Abstinence, Generosity, Relaxation, Gaiety, Inspiration and Strength were important. Concerning 'Sleep Regimen'; Enough sleeping hours, Sound sleep, Curved sleeping posture, and Comfortable bedding were important. Concerning 'Residence Regimen' ; Clean dwelling, Pleasant surroundings, Thatched house life, Living in the village with good-hearted people, and Regular life were important. Concerning 'Health Regimen'; Temperance of drug, use Proper temperature, Proper humidity, and a Clean body were important. Concerning 'Labor Regimen'; Agricultural labor, Diligence, and Domestic labor were important. Concerning Exercise Regimen': Proper exercise, and Sunbathing were important. Concerning 'Being Born' ; Strong physical constitution, Longevity lineage, and Destiny were important. Concerning 'Filial Piety and Posterity' : Showing respect to a long-lived elder and Reducing anxiety were important. Concerning 'Attachment to Life' ; The desire to live long was important. The following conclusion was made from the above results. Human beings cannot avoid death, but the span of life can be prolonged to the maximum span of human life by wisdom, health care, and proper environmental conditions. As a result, a health regimen must be imposed for longevity such as controling food intake, having positive thoughts, being relaxed, sleeping enough, clean dwelling, taking care of oneself which can improve immunity and resistance to decrease, as well as proper labor and exercise. In addition, when filial piety is served, the desire of longevity and retaining one's youthfulness for a long time can be achieved. These should be done to live long and lead a qualitative life. By the results of this research, the following is suggesed for nursing. To satisfy the basic nursing principle that nursing must be practiced to help people live long, education must be spread to people who are nursed and their family so that they can lead a healthy life. And in nursing elders, nurses must recognize that elders have a strong desire for life, even though they may have negative thoughts about life at times. Therefore nurses have to respect the elders' value of life, and help them improve their self-esteem and self -fulfilment.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.5
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• pp.548-557
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• 2014

The purpose of this study was to understand how vegetarian diets in Taiwanese hospitals are well established and provide suggestion for successful implementation in Korea. Micro-ethnographic case study with in-depth interviews, field observations, and paper reviews were used for this study. Experiences of doctors and nutritionists from Taiwanese hospitals where deliver vegetarian hospital diets were analyzed. This research found out Taiwanese life respect culture has influence on introducing vegetarian diets in hospital, besides most Participants agreed to use vegetarian diets for the medical purpose according to their clinic experiences and research results. Meanwhile there is no restriction to compel patients to keep vegetarian diets. All participants answered food has a large effect on the human body and further study on vegetarian diets' efficacy is required. The hospital chief's decision making is crucial to introduce vegetarian diets in hospitals. For the bigger picture, social supports, organizational supports and national policy supports are all important factors for it. The implications and recommendations for Korean hospitals were discussed. The public awareness for vegetarian diet for medical purpose along with research on its efficacy would be needed. In order to make good use of a vegetarian diet for medical purposes, we first need to build a vegetarian-friendly environment, go deep into the study of a diet's effects using diverse methodologies, and combine the diet with traditional medicine theory. Based on the results of this primary study, more related studies should be carried out in the future.

• International Journal of Human Ecology
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• v.9 no.1
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• pp.29-40
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• 2008

This study clarified the classifications of Vietnamese traditional patterns and analyzed the symbolic meanings that reflect the emotional and cultural background of the Vietnamese. The type and symbolism of Vietnamese traditional patterns were analyzed through research on the history of Vietnamese costumes, the history of Vietnamese arts, the history of Vietnamese traditional culture, and a Vietnamese museum survey. The results of this study were as follows: 1. Animal patterns are diverse and include dragons, unicorns, tortoises, phoenixes, cranes, lions, bats, tigers, and buffalo patterns. 2. Dragon patterns are the most utilized pattern for practical purposes. They are often used in royal costumes, Vietnamese traditional dresses, mandarin boots, bamboo fans, silk, satin, ceramics, and other detailed decorations. 3. The patterns symbolized fortune that meant good luck and longevity so people can live long and happy. Then the symbolic meanings of patterns were followed by prosperity, nobleness, apotropism, prolificacy, and wealth. 4. Since the most of animal patterns have symbolic meanings of fortune and longevity, animal patterns can be utilized as the source of traditional patterns of design inspiration for the development of Vietnamese modern patterns and the application on Vietnamese fashion products.

• Herbal Formula Science
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• v.30 no.2
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• pp.59-66
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• 2022

Objectives : This study is to investigate whether Carthami flos exhibits a synergistic effect on the apoptotic effect of doxorubicin on human gastric cancer cells. Methods : We used AGS, a human gastric cancer cell line. To investigate the apoptotic efficacy of doxorubicin and Carthami flos, MTT and CCK-8 methods were used. To confirm apoptosis, cell cycle and mitochondrial membrane potential changes were confirmed. To investigate the mechanism of apoptosis, the reactive oxygen species (ROS) experiment was performed. Results : 1. Doxorubicin or Carthami flos induced cell death in the human gastric cancer cell line AGS. 2. Carthami flos showed a synergistic effect of cell death by doxorubicin. 3. The cell cycle and mitochondrial membrane potential changes revealed that cell death was apoptosis. 4. Apoptosis was related to reactive oxygen species (ROS) generation. Conclusions : This result shows the anticancer synergistic effect of Carthami flos in gastric cancer cells, and is considered to be an important basis for the development of anticancer drugs for Carthami flos.

• Journal of Oriental Neuropsychiatry
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• v.26 no.2
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• pp.103-116
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• 2015

Objectives: Medical students suffer from mental health and academic problems due to academic stress. We investigated academic stress and exhaustion along with psychological issues caused by medical education during the years in the School of Korean Medicine. Methods: Two hundred fifty-one university students from the School of Korean Medicine, Pusan National University, were asked to complete the Medical Stress Scale (MSS), Maslach Burnout Inventory-Student Survey (MBI-SS), Positive Affect and Negative Affect Schedule (PANAS), and State-Trait Anxiety Inventory (STAI) three times during each semester for two years from 2013 onwards. We analyzed the influence of school term and vacation on educational stress and negative affect with the T-test. The Pearson's correlation analysis and regression analysis were used to predict changes during the first semester or first years of study in the School of Korean Medicine. Results: Academic stress and burnout increased steadily until the first semester of third grade, which was measured with MSS and MBI-SS. The anxiety level was highest when the students started the first grade and it decreased significantly after the first semester. Negative affect repeatedly increased significantly after each school term; however, it reduced after each vacation. In the first grade, 19.5% of the last measure of MSS can be explained with the first measure of MSS. Discussion: This study extensively reviewed the trends and characteristics of four years of academic stress and its related psychological influence, and discussed its importance for developing a more efficient academic curriculum for traditional Korean Medicine.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.96-100
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• 2004

Ion exchange polymer membrane in the dehydrated state was found to exhibit bending upon a small applied voltage, although the investigations on the hydrated ion exchange polymer membrane bending behavior have been performed quite intensively for more than a decade for the purpose of producing a practical polymer actuator. Our investigation on the dehydrated ion exchange polymer membrane has revealed that its bending direction is perfectly controllable by the polarity control of applied voltage and the degree of its bending curvature is also almost completely determined by the control of duration time of voltage application on it, while the hydrated ion exchange polymer membranes lack of such properties. Furthermore the longevity of dehydrated ion exchange polymer membrane sustaining such a highly controllable properties has been found quite longer than that of the hydrated ion exchange polymer membrane.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.105-108
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• 2003

To enhance the performance of a serum-free medium (SFM) for human thrombopoietin (hTPO) production in suspension cultures of recombinant Chinese hamster ovary (rCHO) cells, several low-cost hydrolysates such as yeast hydrolysate (YH), soy hydrolysate, wheat gluten hydrolysate and rice hydrolysate were tested as medium additives. Among various hydrolysates tested, the positive effect of YH on hTPO production was most significant. When 5 g/L YH was added to SFM, the maximum hTPO concentration in batch culture was 40.41 ${\mu}g/mL$, which is 11.5 times higher than that in SFM without YH supplementation. This enhanced hTPO production in YH-supplemented SFM was obtained by the combined effect of enhanced $q_{hTPO}$ and increased culture longevity. In addition YH supplementation did not affect in vivo biological activity of hTPO. Taken together, the results obtained demonstrate the potential of YH as a medium additive for hTPO production in serum-free suspension cultures of rCHO cells.

• Journal of Ginseng Culture
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• v.2
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• pp.39-70
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• 2020

In this paper, through the mutual interpretation and verification of the ancient Korean history books with different origin that have been suspected as false documents, it proves that they could be logically real records and reveal that the substance of the legendary 'medicine for anti-aging and longevity', which also had been mentioned in Chinese old books, is Korean ginseng. Furthermore, with reference to the modern Y chromosomal map of the migratory routes of mankind corresponding to these routes recorded in 「Budoji」, the core history book, the formation of the four ethnic constitution groups (Sasang Constitution) based on the life style of each human group has been estimated. And the cause of Korean ginseng with fever problem for Southeast Asians is their pharmacogenomic constitution problem by protopanaxatriol (PPT) type ginsenosides in ginseng. It was resolved with over production of protopanaxdiol (PPD) type ginsenosides against PPT type in Korean red ginseng as historical or scientific point of view. In addition, by explaining that the processing method to Korean red ginseng could increase red ginseng acidic polysaccharides (RGAP), the RGAP, PPD type ginsenosides, and arginine which is originally abundant in Korean ginseng could increase the expression of the 'heat shock proteins' as a kind of chaperone in the body, this paper presents the theory allowing the scientific interpretation of the efficacy of Korean red ginseng as an 'adaptogen' or 'medicine for anti-aging and longevity'. Lastly, through the consideration of the growing environment of American ginseng and Korean ginseng, the differences are presented.