• Title/Summary/Keyword: human embryo kidney-ft cell

Search Result 1, Processing Time 0.017 seconds

Preparation of cross-linked silk fibroin film by γ-irradiation and their application as supports for human cell culture

  • Park, Hyean-Yeol;Kim, Yoon-Seob;Choi, Seong-Ho
    • Analytical Science and Technology
    • /
    • v.27 no.1
    • /
    • pp.27-33
    • /
    • 2014
  • This study described about preparation of the cross-linked silk fibroin (SF) film by ${\gamma}$-irradiation of the casted SF film, which is fabricated from aqueous solution regenerated via fibers of cocoons and their application as supports for human cell culture. The properties of cross-linked SF film were evaluated by FT-IR spectroscopy, contact angle, solubility to water, thermal analysis, surface area analyzer, and morphology via scanning electron microscopy (SEM). The cross-linked SF films were not dissolved in water and exhibited the rough surface morphology, large surface area, and good thermal properties. The human fibroblast cell (CCD-986sk) and embryo kidney-ft cell were well growed on the surface of cross-linked SF film supports prepared by ${\gamma}$-irradiation. The cross-linked SF film prepared by ${\gamma}$-irradiation can be used as biomaterials for human cell culture.