• The Journal of Korean Medical History
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• v.27 no.2
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• pp.99-120
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• 2014

Purpose : There are two types of sleep disturbance: inability to sleep (不眠) and somnolence (多眠). This study is to examine treatments of the two types of sleep disturbance in the Books of Cold Damage and how those theories were established and formed. Methods : 1. Verses including the words related to inability to sleep and somnolence were extracted from "Treatise on Cold Damage Diseases" (傷寒論). 2. Among the Books on Cold Damages that are classified according to the symptoms, 17 books with contents related to inability to sleep and somnolence were selected to collect and classify data in three perspectives: mechanism of disease (病機), method of treatment (治法) and disease pattern (病證). 3. Data collected through the above methods were compared and diagramed. Results & Conclusions : 1. On Cold Damage, inability to sleep is mostly rooted when human body lacks yin energy while having excessive yang energy (陽盛陰虛) due to fire and heat (火熱). The reason could be misuse of perspiration inducing method (汗法) or purgation (下法) on the doctors' part. 2. On Cold Damage, somnolence is rooted when pathogen (邪氣) is spread to yin meridians (陰經) and the human body lacks yang while having excessive yin energy (陰盛陽虛) or when heat (熱邪) is invaded into interior parts (裏部). 3. Many scholars of Cold Damage in later periods had copied the "Treatise on Cold Damage Diseases" for treatments of sleep disturbance but many others have applied the verses from "Treatise on Cold Damage Diseases" or added new treatments. Do jeol-am (陶節庵) and Wang Geung-dang (王肯堂) particularly had deep understanding on "Treatise on Cold Damage Diseases" and utilized the content freely or suggested new remedies because they had thorough knowledge on relating formula as well.

• Smart Structures and Systems
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• v.24 no.5
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• pp.597-606
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• 2019

Damage detection based on dynamic characteristics of a structure is one of important roles in structural damage identification. It is difficult to detect local structural damage using traditional dynamic experimental methods due to a limited number of sensors used in an experiment. In this work, a non-contact test stand of fan blades is established, and a full-field noncontact test method, combined with three-dimensional digital image correlation, Bayesian operational modal analysis, and damage indices, is used to detect local damage of a fan blade under ambient excitation without use of baseline information before structural damage. The methodology is applied to detect invisible local damage on the fan blade. Such a method has a seemingly high potential as an alternative to detect local damage of blades with complex high-precision surfaces under extreme working conditions because it is a noncontact test method and can be used under ambient excitation without human participation.

• Molecules and Cells
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• v.19 no.2
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• pp.167-179
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• 2005

The cells of metazoans respond to DNA damage by either arresting their cell cycle in order to repair the DNA, or by undergoing apoptosis. This response is highly conserved across species, and many of the genes involved in this DNA damage response have been shown to be inactivated in human cancers. This suggests the importance of DNA damage response with regard to the prevention of cancer. The DNA damage checkpoint responses vary greatly depending on the developmental context, cell type, gene expression profile, and the degree and nature of the DNA lesions. More valuable information can be obtained from studies utilizing whole organisms in which the molecular basis of development has been well established, such as Drosophila. Since the discovery of the Drosophila p53 orthologue, various aspects of DNA damage responses have been studied in Drosophila. In this review, I will summarize the current knowledge on the DNA damage checkpoint response in Drosophila. With the ease of genetic, cellular, and cytological approaches, Drosophila will become an increasingly valuable model organism for the study of mechanisms inherent to cancer formation associated with defects in the DNA damage pathway.

• Applied Microscopy
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• v.42 no.3
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• pp.136-141
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• 2012

Diversification and individualization are pursued to fulfill the increasing human desire for beauty. There are many methods to change human appearances; of these, a permanent wave is often applied to improve beauty. A permanent wave uses physical and chemical methods to break and reform the bonds of natural hair. Thus, research into the optimized conditions for permanent waves is necessary to minimize hair damage and improve the efficiency of the treatment. The object of this study is to examine the effect of varying the alkalinity (8%, 12%, and 16%) of the permanent wave treatment on the wave efficiency, degree of hair damage via tensile strength, and degree of hair protein release. The results indicate that the treatment with the highest alkalinity (16%) is more effective than that with 8% alkalinity; however, the degree of hair damage when treated with the 16% alkalinity wave is higher than with the 8% alkalinity wave. Additionally, hair proteins increasingly dissolve with increasing alkalinity of the permanent wave.

• Korean Journal of Human Ecology
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• v.17 no.3
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• pp.553-562
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• 2008

These days hair perm and dye is a modern trend that women prefer to create their own image and personality. However, chemicals for hair perm and dye are easy to damage hair. This study, then, is to find out some effective skills to minimize hair damage when hair perm and dye is done in a same time. The subjects were 200 hairs picked from five healthy women in their twenties which have not been permed and dyed since one year and more ago. The experimental group was divided into two groups: 1)the perm- nutritional treatment- dye (PNT) group 2)the dye- perm- nutritional treatment(DPN) group. After experiments, the states of "hair scale", "hair damage", and "color tone in dyed hair" were compared between two groups. The results are as follows: to lessen hair damage when hair perm and dye is done in a same time, nutritional treatments must be necessarily used on hair. To make hair colored more clearly when hair dye is done, it is more desirable to use nutritional treatments on hair.

• Environmental Analysis Health and Toxicology
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• v.19 no.1
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• pp.101-107
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• 2004

To ascertain the effects of hair dyeing application on the DNA damage in human lymphocytes, a mixture of permanent black colored hair dye with the same amount of oxidant containing 6% hydrogen peroxide was used. A hair dyeing with contacting the scalp (conventional dyeing) and a hair dyeing with 3 to 4mm away from the scalp (alternative dyeing) were applied to each If young healthy women. Blood was taken from the brachial vein at two sampling times, just before and 6 hours after the hair dyeing, and tail extent moment(TEM) and tail length (TL) were measured by using a comet assay. After dyeing, TL was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 47% and 28%, respectively, and TL for conventional dyeing group was higher than alternative dyeing group as an average of 1.2 fold. After dyeing, TEM was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 192% and 76%, respectively, and TEM for conventional dyeing group was significantly higher than alternative dyeing group as an average of 1.7 fold. Therefore, alternative dyeing application was induced to lower lymphocyte DNA damage than conventional dyeing application, and TEM was appeared to be a more sensitive tool for the measurement of lymphocyte DNA damage than TL in this study.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.6
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• pp.805-812
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• 2010

DNA damage including base modifications, loss of base and breaks in DNA strands can occur by exposure to irradiation, smoking and several components of food. Unrepaired DNA damage is known to lead to cellular dysfunction, cell death, cancer, and other diseases such as arteriosclerosis and diabetes. The protective effect of garlic on oxidative stress induced DNA damage has been reported recently. In this study, we investigated the protective effect of garlic extracts prepared by different processing methods (raw garlic extracts, RGE; grilled garlic extracts, GGE; pickled garlic extracts, PGE) on leukocytic DNA damage using comet assay. Human leukocytes were incubated with ethanol and methanol extract of garlic at various concentrations (1, 5, 10, 50 ${\mu}g$/mL), followed by oxidative stimuli (200 ${\mu}M$ $H_2O_2$ or 200 ${\mu}M$ 4-hydroxynonenal (HNE)). The methanol and ethanol extracts of RGE, GGE, and PGE showed inhibitory activities of DNA damage induced by $H_2O_2$ or HNE. Especially methanol extract of RGE ($ED_{50}$; 13.3 ${\mu}g$/mL) had a higher antigenotoxic effect on $H_2O_2$ induced DNA damage than those of GGE (23.5 ${\mu}g$/mL) or PGE (24.5 ${\mu}g$/mL). HNE induced DNA damage tended to be effectively inhibited by the lower concentration of all garlic extracts. Therefore, garlic might have protective effects against oxidative DNA damage regardless of processing methods (raw, grilled, pickled) which are the general consumed forms of garlic in Korea.

• Environmental Mutagens and Carcinogens
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• v.23 no.3
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• pp.99-102
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• 2003

IARC classified areca quid as a human carcinogen. Areca quid chewed in Taiwan includes Piper betle inflorescence, which contains high concentrations of safrole (15 mg/fresh weight). Safrole is a documented rodent hepatocarcinogen, and chewing areca quid may contribute to human exposure (420 $\mu$m in saliva). The carcinogenicity of safrole is mediated through 1'-hydroxysafrole formation, followed by sulfonation to an unstable sulfate that reacts to form DNA adducts. Using human liver microsomes and Escherichia coli membranes expressing bicistronic human P450s, CYP2E1 and CYP2C9 were identified as the main P450s involved in the activation of safrole. We have demonstrated the presence of stable safrole-dGMP adducts in human oral tissues following areca quid chewing using $^{32}$ P-postlabeling and HPLC mass spectrometry methods. By studying 88 subjects with a known AQ chewing history and 161 matched controls, we have demonstrated that the presence of safrole-DNA adducts in peripheral blood cells was correlated to AQ chewing, and CYP2E1 seemed to play an important role in the modulation of safrole-DNA adduct formation. We have also shown that safrole can form stable safrole-DNA adducts as well as oxidative damages in rodent liver. However, the stable safrole-DNA adducts may represent a more significant initial lesion as compared to the rapidly repaired safrole-induced 8-hydroxy-2'-deoxyguanosine. This oxidative DNA damage is mediated through the formation of hydoryxchavicol, the major safrole metabolite in human urine. Hydroxychavicol may have gone through two-electron oxidation to the o-quinone; then via one-electron reduction to semiquinone radicals to generate oxidative DNA damage. However, these reactive metabolites can be efficiently conjugated by GSH. These data suggest that safrole may contribute to the initiation of oral carcinogenesis through safrole-DNA adduct and not oxidative DNA damage. In addition, CYP2E1 may modulate this adduct formation.

• Korean Journal of Veterinary Research
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• v.44 no.1
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• pp.41-47
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• 2004

The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of $^{60}Co$ ${\gamma}$-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p<0.01) and TL (p<0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs. Mouse PBLs were more sensitive than human PBLs which were in turn more sensitive than rat PBLs when the treated dosages were 1 and 2 Gy. However, human PBLs were more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs when the irradiation dosages were 4 and 8 Gy. Data from all three species could be fitted to a linear-quadratic model. These results indicated that there may be inherent differences in the radio-sensitivity among PBLs of mammalian species.

• Journal of the Society of Disaster Information
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• v.3 no.1
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• pp.87-101
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• 2007

The definition and concept of disasters and their preparedness have been changing according to the modern situation. The basic change is that the concept of absolute standard and prevention of hardware damage in the past have been changing to the concept of relative standard and mitigation of direct damage to human. For achieving the purpose, advanced countries developed and used their own analysis method of hazard and vulnerability for disaster ; ASHE hazard and vulnerability evaluation method, hazard matrix method by CDC, FEMA model method and SMUG hazard priority method. Because each analysis method cannot evaluate the hazard and vulnerability for specific disaster, the advantages and disadvantages should be applied for specific situation of disaster in Korea and new analysis method should be extracted in the future.