• Journal of Genetic Medicine
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• v.13 no.1
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• pp.1-13
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• 2016

Although some mutations are beneficial and are the driving force behind evolution, it is important to maintain DNA integrity and stability because it contains genetic information. However, in the oxygen-rich environment we live in, the DNA molecule is under constant threat from endogenous or exogenous insults. DNA damage could trigger the DNA damage response (DDR), which involves DNA repair, the regulation of cell cycle checkpoints, and the induction of programmed cell death or senescence. Dysregulation of these physiological responses to DNA damage causes developmental defects, neurological defects, premature aging, infertility, immune system defects, and tumors in humans. Some human syndromes are characterized by unique neurological phenotypes including microcephaly, mental retardation, ataxia, neurodegeneration, and neuropathy, suggesting a direct link between genomic instability resulting from defective DDR and neuropathology. In this review, rare human genetic disorders related to abnormal DDR and damage repair with neural defects will be discussed.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.2
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• pp.90-98
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• 2023

The human eye, constantly exposed to solar radiation, can be damaged by UV radiation. In particular, ultraviolet B (UVB)-induced damage plays an important role in retinal degeneration and cell aging. In this study, we investigated the protective effects of the methanol extract of Oxya chinensis sinuosa (OCM), an edible insect known for its high protein content (64.2%), and various pharmacological effects, on human retinal pigment epithelial cells. ARPE-19 cells were treated with OCM and subsequently UVB irradiated. Our results showed that OCM effectively attenuates UVB-induced cell damage by reducing MAPK phosphorylation (JNK and p38 MAPK). Additionally, OCM increased the phosphorylation of Akt, and cell cycle regulators, including p21 and p27, in a dose-dependent manner. Moreover, OCM treatment increased ARPE-19 cell proliferation by activating the S6K1/S6 pathway. This study suggests that OCM prevents UVB-induced retinal cell damage by increasing cell proliferation via ROS reduction, suggesting its potential as a functional therapeutic superfood against retinal cell damage.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.31 no.11
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• pp.936-941
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• 2007

This paper is on the influence of gas explosion caused by Vapor Cloud Explosion(VCE). Also, it is to understand the influence of the booth for explosion experiment which is installed to let the trainees for legal education which is managed by IGTT(Institute or Gas Technology Training) know the riskiness of explosion. In this study, the influence of explosion shock wave caused by VCE in enclosure was calculated by using the Hopkinson's scaling law and the accident damage was estimated by applying the influence on the adjacent human into the probit model. As a result of the damage estimation conducted by using the probit model, both the damage possibility of explosion overpressure to human 8 meters away and that of shock wave to hurt 15 meters away showed nothing.

• Journal of Digital Convergence
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• v.17 no.6
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• pp.51-57
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• 2019

The objective of the study was to determine the mediation effects of hope and grit in the relationship between human rights damage and self-esteem. A sample of students was recruited from two universities in Korea. For data analysis, SPSS PC+ and SPSS PROCESS macro were used. Frequency, reliability, correlation and mediating effect analysis were performed. Bootstrap technique was done to verify the mediating effect. Results showed as follows. First, grit, self-esteem and hope were negatively correlated with human rights damage, but grit, self-esteem and hope were positively correlated with each other. Second, hope and grit mediated in the relationship between human rights damage and self-esteem. For further research, it was discussed how university students who experienced human rights abuses could use grit and hope to maintain their lowered self-esteem.

• Explosives and Blasting
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• v.22 no.3
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• pp.85-95
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• 2004

A damage criterion by blast vibration & noise has to be established through a engineering view point. Though a blast vibration has effects on human and structure, they have experienced a different damage. Thus, a damage criterion for structure needs to get a peculiar criterion which are different from one for human. At present, blast damage criterions authorized by government are Vibration & Noise Act made by Ministry of Environment and blast damage criterion of specifications for tunnel made by Ministry of Construction & Transportation. Vibration & Noise Act is based on the response of human, and while it has some application problems, it turns out to be partly rational. But, blast damage criterion of specifications for tunnel has unreasonable guidelines. In this article, its problems are reviewed and a alternative blast damage criterion is suggested.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.302-311
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• 2008

There have been repetitive landslides and debris flows on natural terrain induced by intensive rainfalls which have never been experienced during the last a few decades in Korea. Frequencies and magnitudes of landslides and debris flows are steeply increased after 2000 resulting in huge damages of human beings and facilities. According to a statistical data from NEMA, the human deaths induced by landslides and slope hazards occupies 22.3% of the total human deaths by all the natural hazards in Korea during the last 30 years. Among the human deaths by landslides and slope hazards, 85% of the damages were caused by landslides and debris flows on natural hazards. Therefore, this paper summarizes important events of landslides and debris flows, their characteristics, and suggests some methods of damage mitigation.

• Proceedings of the Korean Society of Toxicology Conference
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• 2003.05a
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• pp.38-38
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• 2003

High iron consumption is associated with an increased risk of cancer possibly via production of reactive oxygen species (ROS) which in turn induces oxidative damage to lipids, proteins and DNA. The aim of the study was to determine whether Fe-NT A causes DNA damage and targets TP 53 in human peripheral lymphocytes. (omitted)

• The Korean Journal of Physiology and Pharmacology
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• v.27 no.5
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• pp.427-436
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• 2023

Mitotic arrest deficient 2 like 2 (Mad2L2, also known as Mad2B), the human homologue of the yeast Rev7 protein, is a regulatory subunit of DNA polymerase ζ that shares high sequence homology with Mad2, the mitotic checkpoint protein. Previously, we demonstrated the involvement of Mad2B in the cisplatin-induced DNA damage response. In this study, we extend our findings to show that Mad2B is recruited to sites of DNA damage in human cancer cells in response to cisplatin treatment. We found that in undamaged cells, Mad2B exists in a complex with Polζ-Rev1 and the APC/C subunit Cdc27. Following cisplatin-induced DNA damage, we observed an increase in the recruitment of Mad2B and Cdc20 (the activators of the APC/C), to the complex. The involvement of Mad2B-Cdc20-APC/C during DNA damage has not been reported before and suggests that the APC/C is activated following cisplatin-induced DNA damage. Using an in vitro ubiquitination assay, our data confirmed Mad2B-dependent activation of APC/C in cisplatin-treated cells. Mad2B may act as an accelerator for APC/C activation during DNA damage response. Our data strongly suggest a role for Mad2B-APC/C-Cdc20 in the ubiquitination of proteins involved in the DNA damage response.

• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.111-115
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• 2005

Ultraviolet (UV) radiation to mammalian skin is known to alter cellular function via generation of Reactive Oxygen Species (ROS), DNA damage and DNA lesions, such as pyrimidine dimmers and photoproducts, which could lead to DNA mutation if they are not repaired. In this study, we have investigated the reduction of DNA damage and of apoptosis with a particular attention to genetic effect of paeoniflorin in Normal Human Epidermal Keratinocytes (NHEK). After UVB irradiation from $10\;to\;500mJ/cm^{2}$ to NHEK, Mean Tail Moments (MTM) were increased with UVB dose increase. The greatest amount of strand breaks was induced at $500mJ/cm^{2}$ of UVB. Even at the lowest dose of UVB ($10mJ/cm^{2}$), change in MTM was detected (P<0.0001). Pretreated cell with 0.1% paeoniflorin maximally reduced the level of DNA damage to about 21.3%, compared to untreated cell. In the lower concentrations less than 0.01% of paeoniflorin, MTM had a small increase but paeoniflorin still had reductive effects of DNA damage. We measured the apoptosis suppression of paeoniflorin with annexin V flous staining kit. As we observed under the fluorescence microscopy to detect apoptosis in the irradiated cell, the fluorescence intensity was clearly increased in the untreated cell, but decreased in treated cells with paeoniflorin. These results suggest that paeoniflorin reduces the alteration of cell membranes and prevents DNA damage. Therefore, the use of paeoniflorin as a free radical scavenger to reduce the harmful effects of UV lights such as chronic skin damage, wrinkling and skin cancer can be useful to prevent the formation of photooxidants that result in radical damage.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.24 no.68
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• pp.51-65
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• 2001

This study is to make LCIA(Life Cycle Impact Assessment) easier as a methodology of environmental scores(called E-score) that integrated environmental load of each emission substance based on environmental damage such as in human health, ecosystem and resources category. The concept is to analyzes the LCI(Life Cycle Inventory) and defines the level of environment damages for human health, ecosystem and resources to objective impact assessment standard, and makes the base of marginal damage to calculate the damage factor, which can present the indication that can establish the standard value of environmental impact. First, damages to human health are calculated by fate analysis, effect analysis and damage analysis to get the damage factor of health effect as a DALY(Disability Adjusted Life Years) unit. Second, damages to ecosystem are calculated by fate analysis, effect analysis and damage analysis to get the damage factor of the effect as a PDF(Potentially Disappeared Fraction) unit through linking potentially increased disappeared fraction. Third, damages to resources are carried out by resource analysis and damage analysis for linking the lower fate to surplus energy conception to get damage factor as a MJ(Mega Joule) unit. For the ranking of relative environment load level each other, LCIA can be carried out effectively by applying this E-score methodology to the particular emission substances. A case study has been introduced for the emission substances coming out of a tire manufacturer in Korea. It is to show how to work the methodology. Based on such study result, product-designers or producers now can apply the E-scores presented in this study to the substances of emission list, and then calculate the environment load of the product or process in advance at any time and can see the environment performance comparatively and expected to contribute to the environmental improvement in view of environmental pollution prevention.