• Journal of Photoscience
• /
• v.9 no.2
• /
• pp.150-153
• /
• 2002

Solar UV light is a well-known carcinogen. UVA radiation is probably carcinogenic to humans. In addition, recent investigations point to the importance of UVA irradiation in the photoaging. We investigated the mechanism of sequence- specific DNA damage using $\^$32/P-Iabeled DNA fragments in relation to carcinogenesis and aging. Furthermore, we investigated whether UVA accelerates the telomere shortening in human WI-38 fibroblasts. The exposure of double- stranded DNA fragments to 365 nm light in the presence of endogenous sensitizers produced sequence-specific cleavage at the 5' site of 5'-GG-3' and 5'-GGG-3' sequences. In addition, HPLC analysis revealed that sensitizers plus 365 nm light increased the 8-oxodG content of double-stranded DNA. We discuss the mechanisms of guanine-specific DNA damagecaused by excited photosensitizers in relation to carcinogenesis and aging.

• Journal of Korean Society for Atmospheric Environment
• /
• v.2 no.3
• /
• pp.19-26
• /
• 1986

The degree of air pollution is usually presented in terms of concentration. But, in evaluating the environmental impacts or air pollution control policies, the concentration values need to be interpreted in terms of damage effects on property and human health. The damage effect varies with the types of pollution, subjects and land use pattern of an affected area. Therefore, this study is aimed at developing a method of analyzing effects of various types of air pollutions on surrounding environmental setting with the EIMS (Environmental Information Management System) developed for land suitability analysis. Using the method formulated in this study, the long- term effects of such pollutants as $SO_2$ and HF on types of vegetation and residents, and potential, short-term effects of HCl leak accidents from manufacturing facilities in Ulsan and Onsan Industrial complex are analyzed. The presentation of the damage effects of air pollution rather than the concentration of pollutants will be useful for the preparation of environmental impact statements, the formulation of environmental policies, and the development of land use plans in heavily industrialized areas.

• Earthquakes and Structures
• /
• v.7 no.6
• /
• pp.1045-1060
• /
• 2014

The 2011 off the Pacific coast of Tohoku Earthquake brought serious damage around the Tohoku district in Japan, and much human life and fortune were lost. Bridges were damaged by this earthquake. It was the most serious damage that the superstructures of bridges were flowed out by tsunami. Earthquakes of the same scale are predicted in other areas of Japan. It is necessary to take measures for bridges near coast. In order to understand the tsunami force acting on the bridge, hydraulic model experiments was conducted. In addition, this paper focused on fairing that is effective in wind resistant stability. Installing fairing to bridges has been verified by experiments whether it is possible to reduce the force of tsunami.

• Journal of the Korean Professional Engineers Association
• /
• v.45 no.1
• /
• pp.45-48
• /
• 2012

The 2011 Japan Tsunami caused tremendous damage to coastal areas. Because of their drastic propagation speed and large run-up height, nearshore tsunami can cause catastrophic damages on coastal communities within a short time. It is necessary to establish the tsunami hazard mitigation to reduce human injury housing damage. The construction of Tsunami warning system and production of hazard map are needed for minimizing damage by tsunami.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
• /
• 2009.05a
• /
• pp.340-343
• /
• 2009

Recently, the lightning damage to human and structure is increasing steadily and many countries try to reduce lightning-caused casualties and economical loss through special disaster management system. The organization of warning system and management system for lightning damage in developed countries were investigated in this paper. The organization of central government for disaster management and cooperation system with local government in USA, Japan, the Britain, and Germany were introduced and the division of roles with meteorological administration were described.

• Journal of the Architectural Institute of Korea Planning & Design
• /
• v.34 no.8
• /
• pp.129-136
• /
• 2018

Termites are important sub-baits of natural ecosystems and are beneficial insects that are natural decomposer. However, the building materials used by human beings can not coexist with each other on timber which is a termite food. As of 2017, due to fertile forests, warming, and the spread of boilers, termite damage to timber construction is increasingly occurring day by day. Currently, Korea utilizes fumigation, Beit system, preserved wood, etc. of chemical products as termite control measures. But in the case of control measures, timber that has already been eaten by termites can not serve as a structural material and must be repaired. On the other hand, in the case of overseas, Scale of damage to termites the influence on the construction is also large, and a lot of research has been carried out on the prevention methods. In this thesis, investigate such foreign control measures, Would like to suggest a suitable method for Korea.

• Toxicological Research
• /
• v.8 no.2
• /
• pp.285-302
• /
• 1992

Human and rat hepatocytes were isolated by nonperfusion method and cultured for longer than 5 days. Human liver biopsy sample and rat liver were used as hepatocyte source. Several physical and chemical factors which were influencing on hepatocyte isolation procedure were examined and a batch isolation procedure was established for small size sample of rat liver. Isolated hepatocytes showed normal morphlologica characteristics in microscopy and electron microscopical examinations and a morphologica response to phalloidin. Isolated cells were cultured as a monolayer and proven to have intact morphological characteristics for longer than 15 days. Because human liver sample is harder and tighter compared with rat liver, a standard procedure for rat hepatocytes was slightly modified to reduce mechanical damage. Similarly with rat hepatocytes, isolated human hepatocytes showed a normal morphological characteristics and could be cultured for longer than 15days. Human and rat hepatocytes were examined on their functional integrities including cytochrome-P450 related enzyme activity and it's inducibility, hormonal inducibility of AIB uptake and TAT activity, albumin synthesis, DNA synthesis, cellular protein maintenance. In all parameters used in the present study, human and rat hepatocytes showed normal functional characteristics.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.3
• /
• pp.633-643
• /
• 2017

To examine the pro-apoptotic role of the human ortholog (YPEL5) of the Drosophila Yippee protein, the cell viability of Saccharomyces cerevisiae mutant strain with deleted MOH1, the yeast ortholog, was compared with that of the wild-type (WT)-MOH1 strain after exposure to different apoptogenic stimulants, including UV irradiation, methyl methanesulfonate (MMS), camptothecin (CPT), heat shock, and hyperosmotic shock. The $moh1{\Delta}$ mutant exhibited enhanced cell viability compared with the WT-MOH1 strain when treated with lethal UV irradiation, 1.8 mM MMS, $100{\mu}M$ CPT, heat shock at $50^{\circ}C$, or 1.2 M KCl. At the same time, the level of Moh1 protein was commonly up-regulated in the WT-MOH1 strain as was that of Ynk1 protein, which is known as a marker for DNA damage. Although the enhanced UV resistance of the $moh1{\Delta}$ mutant largely disappeared following transformation with the yeast MOH1 gene or one of the human YPEL1-YPEL5 genes, the transformant bearing pYES2-YPEL5 was more sensitive to lethal UV irradiation and its UV sensitivity was similar to that of the WT-MOH1 strain. Under these conditions, the UV irradiation-induced apoptotic events, such as FITC-Annexin V stainability, mitochondrial membrane potential (${\Delta}{\psi}m$) loss, and metacaspase activation, occurred to a much lesser extent in the $moh1{\Delta}$ mutant compared with the WT-MOH1 strain and the mutant strain bearing pYES2-MOH1 or pYES2-YPEL5. These results demonstrate the functional conservation between yeast Moh1 and human YPEL5, and their involvement in mitochondria-dependent apoptosis induced by DNA damage.

• Proceedings of the Society of Korea Industrial and System Engineering Conference
• /
• 2002.05a
• /
• pp.25-30
• /
• 2002

The problem of qualitative reliability system is very important issue in the digitalized nuclear power plant, because the failure of its system brings about extravagant economic loss, extensive environment destruction, and fatal damage of human. Therefore this study is to develop the reliability evaluation model through the normalized scoring model by the quantitative and qualitative factors considering the advanced safety factors In the Advanced Pressurized water Reactor 1400MWe(APR 1400) under uncertainty Especially, the qualitative factors considering the information and human factors for the systematic and rational justification have been closely analyzed. The reliability evaluation model can be simply applied in real fields in order to minimize the industrial accident and human error in the digitalized nuclear power plant.

• Environmental Mutagens and Carcinogens
• /
• v.15 no.2
• /
• pp.81-87
• /
• 1995

The clastogenic activity of capsaicin, a major pungent and irritating constituent of hot chili pepper, was evaluated in cultured human lymphocytes. Capsaicin (125, 250, and 500 $\mu$M) caused cytogenetic damage as determined by increased frequency of chromosome/chromatid aberrations compared to the solvent control. The mitotic indices were also decreased in a concentration-related manner in capsaicin-treated cells. Moreover, capsaicin suppressed [$^3$]thymidine incorporation into lymphocytes. The clastogenicity and cytotoxicity of capsaicin towards human lymphocytes were evident without an external metabolic activation system. Taken together, these findings suggest that capsaicin is a genotoxic agent and may thus represent a potential health hazard in humans.