• Journal of The Korean Society of Integrative Medicine
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• v.11 no.2
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• pp.169-179
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• 2023

Purpose : Although human periodontal ligament stem cells (hPDLSCs) are a supportive factor for tissue engineering, oxidative stress during cell culture and transplantation has been shown to affect stem cell viability and mortality, leading to failed regeneration. The aim of this study was to evaluate the antioxidant and protective effects against cell damage of celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, and the antioxidant signal of hPDLSCs in H₂O₂-induced oxidative stress. Methods : To induce oxidative stress in cultured hPDLSCs, H₂O₂ was used as an exogenous reactive oxygen species (ROS). Dose-dependent celecoxib (.1, 1, 10, or 100 µM) was administered after H₂O₂ treatment. WST-1 assay was used to assess cell damage and western blot was used to observe antioxidant activity of hPDLSCs in oxidative stress. Immunohistochemistry was performed for inverting the localization of the SOD and Nrf2 antibody. Results : We found that progressive cell death was induced in hPDLSCs by H₂O₂ treatment. However, low-dose celecoxib reduced H₂O₂-induced cellular damage and eventually enhanced the SOD activity and Nrf2 signal of hPDLSCs. Oxidative stress-induced morphological change in hPDLSCs included lowered the survival and number of spindle-shaped cells, and shrinkage and shortening of cell fibers. Notably, celecoxib promoted cell survival function and activated antioxidants such as SOD and Nrf2 by positively regulating the cell survival signal pathway, and also reduced the number of morphological changes in hPDLS. Immunohistochemistry results showed a greater number of SOD- and Nrf2-stained cells in the celecoxib-treated group following oxidative stress. Conclusion : By increasing SOD and Nrf2 expression at the antioxidant system, the findings suggest that celecoxib enhanced the antioxidative ability of hPDLSCs and protected cell viability against H₂O₂-induced oxidative stress by increasing SOD and Nrf2 expression in the antioxidant system.

• Journal of the Korean Society of Safety
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• v.30 no.1
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• pp.87-93
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• 2015

Most incidents and accidents involved human during operating NPPs have a tendency to be structured by complicated and various organizational, individual, and environmental factors. The salient feature of the human error in NPP was extremely low frequency, extremely high complicated and extremely serious damage of human life and property. Our research team defined as 'rare human errors'. To prevent the rare human errors, the most researchers and analysts insist invariably that the root causes be made clear. The making them clear, however, is difficult because their root causes are very various and uncertain. However, These tools have limits that they do not adapt all operating situations and circumstances such as design base events. The purpose of this study is to improve the rare human error hazards consider the situational contex. Through this challenging try based on evidences to the human errors could be useful to prevent rare and critical events can occur in the future.

• Journal of the Korean Geographical Society
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• v.50 no.3
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• pp.355-373
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• 2015

In this study the factors of fire damage are analyzed through previous research reviews. Local environmental factors as well as those factors attributed to fire damage (number of fire events, number of injured, number of death, economic loss) were selected to compose mutual relationship model. In order to verify this relationship model, official statistics concerning fire damage were collected from 228 local governments and compared with results from previous research. As a result of this comparison four dependent variables and 22 independent variables that affect fire damage were analyzed. Independent variables are divided into human vulnerability factors, physical vulnerability factors, economic vulnerability factors, mitigating factors and local characteristics. To analyze a relationship between selected dependent variables and independent variables, we applied a semi-logarithm model and performed regression analysis. Among the 22 independent variables, the number of the weak to disaster, social welfare service workers, workers in manufacturing industry, and the number of workers in restaurants and bars per 10,000 people show the significant correlation with the number of fire incidence. The number of death from fire is significantly related to two variables which are the number of social welfare service workers per 10,000 and the ratio of commercial area. Damage cost is significantly dependent on the property taxes per 10,000 people. These factors were included in the research model as vulnerability factors (human, physical, economic) and mitigating factors and local characteristics, and the validity of research model was verified. The result could contribute to fire-fighting resource allocation in Korea or they can be utilized in establishing fire prevention policy, which will enhance the national level of fire safety.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.29 no.4
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• pp.124-133
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• 2021

The airline industry has been growing steadily since 2016 with more than 100 million air passengers, renewing the largest number of air passengers every year. Increasing air demand leads to an increase in air traffic in limited airspace, increasing the likelihood of accidents between aircraft. Due to the massive human and material damage caused by a single mistake, aviation safety is being heavily focused around the world to efficiently use limited airspace. Studies related to various human factors are underway as most of the aviation accidents are found to be caused by human factors, but research on human factors by controllers is insufficient while they are active in terms of control and operation. Given that 82% of air accidents caused by controllers are caused by human error, the importance of management of human error and changes in perception are urgently needed. This study aims to understand the seriousness of the controller's human error by analyzing the accident cases caused by the controller's human error using TEM to identify threats and errors and derive common human factors.

• Journal of Preventive Medicine and Public Health
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• v.35 no.4
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• pp.275-281
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• 2002

Objectives : To evaluate the DNA damage by hair dyeing in human lymphocytes Methods : Comet assays were carried out to evaluate the DNA damage in lymphocytes by hair dyeing. Twenty subjects were selected from women volunteers whose age ranged from 55 to 67 year old. All subjects had no smoking history. Blood samples were collected before and 6 hours after hair dyeing. DNA damage was evaluated by means of the tail moments, which were quantified by a KOMET 4.0 image analysis system. Results : The tail moments before hair dyeing showed no significant differences among subjects except for the high frequency group. The mean values of the tail moments in subjects with low and high frequencies of hair dyeing were 1.39 and 1.77, respectively (p<0.05). The tail moments after hair dyeing increased significantly, The mean values of tail moments in subjects before and after hair dyeing were 1.45 and 1.79, respectively (p<0.01). However, the difference levels of DNA damage in lymphocytes before and after hair dyeing were found to be slightly lower in both the dietary supplement taking group and high frequency group. Conclusions : The high frequency group appears to have a higher level of DNA damage than the low frequency group before hair dyeing. DNA damage in lymphocytes was found to be significantly higher in the volunteers after hair dyeing. In this study, the related factors such as high frequency and taking dietary supplements appeard to reduce DNA damage in lymphocytes after hair dyeing.

• Journal of Radiation Protection and Research
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• v.33 no.2
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• pp.67-76
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• 2008

As the first comprehensive attempt at a national implementation, this study aims at assessing the external costs of major electricity generation technologies in Korea, particularly an evaluation of the impacts on human health resulting from exposures to atmospheric radiological emissions from nuclear power plants, and a monetary quantification of their damages. The methodology used for the assessment of the externalities of the selected fuel cycles has been developed by the International Atomic Energy Agency (IAEA), namely the SimPacts Model Package. The model is internationally recognized as a tool which can be applied to a wide range of fuels, different technologies and locations, for an externalities study. In this study, the relevant emissions are quantified first and then their impacts on human health are evaluated and compared. The study focused on all the nuclear power plants for the last 6 years ($2001{\sim}2006$) in Korea. With respect to nuclear power, the impact analysis only focuses on a power generation, however the front- and back-end nuclear fuel cycles are not included, namely uranium mining, conversion, enrichment, reprocessing, conditioning, etc., because these facilities are not present in Korea. The analysis results show that nuclear power in general, generates low external costs. The highest damage costs from the nuclear power plants among the 4 sites in Korea were estimated to be 3.9 mills/MWh, which is about 1/20th of the result for a similar case study conducted in the U.K., implemented through the ExternE project. This difference is largely due to the number of radionuclides included in the study and the amount of released radioactive emissions based on up-to-date information in Korea. In this study, the sensitivities of the major factors for nuclear power plants were also calculated. The analysis indicates that there was around a ${\pm}3%$ damage costs variation to a ${\pm}15%$ change of the reference population density and a ${\pm}1%$ damage cost variation to a $1{\sim}30$ meters change of the effective release height, respectively. These sensitive calculations show that there is only a minor difference when the reference costs are compared.

• Journal of Life Science
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• v.33 no.5
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• pp.414-421
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• 2023

Cnidium japonicum (C. japonicum) is a type of halophyte that inhabits soil of a high salinity, and according to previous studies, it is known to have antitumor effects. However, the skin's protective effect, particularly against UVB irradiation, has not been revealed. In this study, C. japonicum crude extract was studied to determine its effect on damage to human keratinocytes (HaCaT) induced by UVB irradiation, and ROS assays were performed, the results of which showed that C. japonicum crude extract affects UVB-induced photoaging damage in human keratinocytes. To examine inhibitory effects against the expressions of MMPs, RT-PCR and Western blot assay were performed by treating the crude extract at concentrations of 10, 50, and 100 ㎍/ml by irradiating UVB at 15 mJ/cm². As a result, it was confirmed that the mRNA and protein expression levels of MMP-1, MMP-3, and MMP-9 decreased in the group treated with C. japonicum crude extract, which also effectively regulated the antioxidant defense mechanism pathway by activating JNK, ERK, and p38. In conclusion, the current study suggested the possibility that C. japonicum could be used as a raw material for anti-photoaging cosmeceuticals in the future.

• Toxicological Research
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• v.23 no.1
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• pp.25-31
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• 2007

To evaluate the protective effect of Houttuynia cordata on hydrogen peroxide-induced oxidative DNA damage in HepG2 cell line, we used an alkaline single-cell gel electrophoresis (SCGE; comet assay). The DNA damage was analyzed by tail moment (TM) and tail length (TL), which used markers of DNA strand breaks in SCGE. The $100{\mu}g/ml$ of methanolic extract of Houttuynia cordata root showed significant protective effects (p < 0.01) against hydrogen peroxide-induced DNA damage in HepG2 cells and increased cell viability against hydrogen peroxide. The results of this study indicate that Houttuynia cordata root methanol extract acts as a potential antioxidant, and exhibits potential anticancer properties, which may provide a clue to find applications in new pharmaceuticals for oxidative stability.

• Journal of Nutrition and Health
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• v.27 no.7
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• pp.740-751
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• 1994

Sister chromatid exchanges(SCE) in peripheral lymphocytes is recently used as a biomarker for increased cytogenetic damage in smokers. The purpose of the investigation was to determine if there were any relationships between dietary factors and their DNA damage as measured by SCE test in a group of 62 male cigarette smokers and 36 non-smokers. As expected, smokers as compared with non-smokers had high SCE levels (10.59$\pm$0.21 versus 9.23$\pm$0.17 SCE/lymphocytes ; p<0.05). No significant relationships were observed between SCEs and age in smokers and non-smokers. In smokers, SCEs were negatively correlated with egg frequency score(r=-0.336) and total food frequency scores(r=-0.283). In non-smokers, SCEs were positively correlated with white vegetable frequency score(r=0.333) and instant food frequency score(r=0.382). There was a positive association between SCEs and the history of coffee intake of smokers(r=0.318). SCE frequency was not influenced by any other dietary factors considered ; dietary diversity and quality scores, alcohol consumption, use of processed foods and intake of burned food. No significant relationships were found between SCEs and serum cholesterol or other hematological parameters of the subjects. These results indicate that increased egg frequency score, total food frequency score which reflects dietary quality, and decreased coffee intake may reduce cancer risk by preventing smoking-induced DNA damage as reflected by sister chromatid exchanges in human lymphocytes.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.720-723
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• 2004

This study is intended to propose a systematic approach for determining optimum Life-Cycle Cost (LCC)-effective seismic design for continuous PSC bridges considering lifetime expected seismic risks. In the paper, a set of cost function for LCC analysis of bridges is proposed. The total LCC functions consist of initial cost and direct/indirect damage costs considering repair/replacement costs, human losses and property damage costs, road user costs, and indirect socio-economic losses. The damage costs are expressed in terms of Park-Ang median global damage indices (Park and Ang, 1985) and lifetime damage probabilities. The proposed approach is applied to model bridges of both moderate seismicity regions like Korea and high seismicity regions like Japan. Since, in case of bridges, a number of parameters may have an influence on optimal target reliability, various sensitivity analyses are performed in this study. It may be expected that the proposed approach can be effectively utilized for the development of cost-effective performance criteria for design and upgrading of various types of bridges as well as continuous PC bridges.