• Parasites, Hosts and Diseases
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• 제28권1호
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• pp.25-30
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• 1990

질트리코모나스(Trichomonas vaginalis)감염 환자 30명과 비감염 건강 대조군 30명을 패 상으로 혈청 내 항-질트리코모나스 IgG 및 IgM 항체가를 효소표식 면역검사법(enzyme-linked immunosorbent assay: ELISA)으로 측정하여 이 질환 진단법으로서 ELISA의 이용 가능성을 검토 하고, 현펑 내 IgG 및 IsM 항체가의 상관성을 알아보았다. 질트리코모나스 환자의 혈청 내 IgG 항체가는 0.37±0.134로 건강 대조군의 0.21±0.054 보다 유 의하게 높았다(p<0.005). 또 혈청 내 IgG 항체가 0.32 이상을 양성으로 하였을 때 ELISA 진단법 의 감수성은 70.0%이었고 특이성은 96.7%이었다. 질트리코모나스 환자의 혈청 내 IsM 항체가는 0.33±0.177로 건강 대조군의 0.11±0.051 보다 유의하게 높았다(p<0.005). 또 혈청 내 Is변 항체 가 0.21 이상을 양성 반응으로 하였을 때 감수성은 70.0%이었고 특이성은 96.7%이었다. 질트리코 모나스 환자의 혈청 내 IgG 와 IsM 항체가의 상관 관계는 상관계수 r=0.77로 유의한 상관관계가 인 정 되 었 다(p<0.005). 이상의 결과로 보아 질트리코모나스 환자에서 혈청 내 19G 및 IsM 항체가를 ELISA법으로 측정 함으로써 질트리코모나스 감염을 진단하거나 역학적 조사에 이용할 수 있을 것으로 생각된다.

• 대한핵의학회지
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• 제27권2호
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• pp.270-276
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• 1993

$Technetium-^{99m}$ labeling method using bifunctional chelating agent cyclic DTPA has been evaluated with human polyclonal nonspecific IgG. IgG was conjugated with cyclic DTPA with various molar ratio. Reduction of $^{99m}Tc$ was done with $Na_2S_2O_4$ with various molar excess. Labeling efficiency and identification of polymer was confirmed with HPLC using TSK4000 SW column. Polymer was purified with 100 cm Sepharose 6LB column. Cultured $1{\times}10^9$ Staphylococcus aureus were injected into rat thigh 24 hours later labeled IgG was injected, and in vivo distribution was observed 4 and 24 hours thereafter. Reduction of $^{99m}Tc$ was optimal with the 10000-50000 times molar excess of $Na_2S_2O_4$. Polymer formation increased with increasing mloar excess of cyclic DTPA to IgG. Three step labeling-labeling DTPA conjugated IgG after reduction of $^{99m}Tc$-made more polymer than two two step labeling-simultaneous mixing DTPA conjugated IgG, $^{99m}Tc$ and $Na_2S_2O_4$. $^{99m}Tc$ blood clearance and lower uptake in the abscess and other organs. IgG conjugated with 200 times molar excess of cyclic DTPA showed slower blood clearance with 200 times molar excess of cyclic DTPA showed slower blood clearance than that of 200 times molar excess of cyclic DTPA showed slower blood clearance than that of 20 times molar excess. In the $^{99m}Tc$ labeling of IgG with cyclic DTPA for the immunoscintigraphy, obtimal labeling condition should be chosen, and effect of the $^{99m}Tc$ labeled IgG polymer should be considered.

• 대한핵의학회지
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• 제28권1호
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• pp.106-111
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• 1994

2-iminothiolane is known to bind $NH_2$ group of lysine in the protein and deliver SH group, which can be used to label protein with $^{99m}Tc$. In this study, we looked for the best reaction condition in which 2-iminothiolane is conjugated to human polyclonal IgG and labeling condition with $^{99m}Tc$-glucoheptonate. Labeling yield was measured with TSK G4000SW column and HPLC or precipitation with 10% TCA (trichloroacetic acid) and 1% HSA. In vivo distribution was investigated with Staphylococcal abscess bearing rats. With decreasing glucoheptonate, the labeling yield decreased. Without 2-iminothiolane, $^{99m}Tc$-glucoheptonate was bound to IgG, which seemed to be direct labeling. With increasing 2-iminothiolane upto 20 times higher than IgG, the labeling yield increased, and plateau was seen with higher molar excess of 2-iminothiolane. Polymer formation was not observed. The pH for the conjugation of 2-iminothiolane and IgG was best around 6.4. $^{99m}Tc$-2-iminothiolane-IgG showed faster blood clearance, higher renal activity and lower hepatic and splenic activity than $^{99m}Tc$-DTPA-IgG. The biodistribution of $^{99m}Tc$-2-iminothiolane-IgG with higher molar excess of 2-iminothiolane was not different from that with lower molar excess. Labeling antibodies with $^{99m}Tc$ using 2-iminothiolane can afford a possible route to simple labeling and wide clinical use of the immunoscintigraphy.

• 센서학회지
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• 제7권4호
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• pp.263-270
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• 1998

본 연구에서는 특정한 생물학적 의미를 갖는 물질들간의 결합 및 분리과정을 실시간에 측정하기 위해 빠른 응답특성과 높은 감도를 갖는 표면 플라즈몬 공명 (Surface Plasmon Resonance, SPR) 현상을 이용한 센서 시스템을 제작하였다. SPR 시스템의 프리즘 표면에 일정 두께의 금 박막이 진공증착된 센서칩을 두고 이 아래에 위치한 시료충전셀에 살모넬라 (salmonella) 항체를 주입시켜 항체가 센서칩 표면에서 자기집합 (self-assembly) 함에 따라 공명각이 변화하는 현상을 측정하였다. 이후 분석대상물질인 살모넬라 항원을 주입하여 항체와의 결합 상태를 일정 시간 간격을 두고 공명각의 변화로서 측정하고 이 결과를 분석하였다. 또한 human Immunglobulin G (hIgG)를 항원으로 한 항원-항체 반응 역시 살모넬라의 경우와 같은 방법으로 측정하였다. 그 결과 살모넬라의 항체는 센서칩 표면에서 약 10분 동안 자기집합하고 반응이 포화됨을 공명각의 변화를 통해 볼 수 있었으며, hIgG의 항체의 경우는 약 60분 동안 반응을 하고 포화됨을, 그리고 살모넬라와 hIgG의 항원 (분석대상물질) 은 모두 각각의 항체에 대해서 약 1분 이내에 결합하고 포화됨을 볼 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제18권8호
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• pp.1393-1400
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• 2008

Recent study has demonstrated an increasing prevalence of food allergy in Korean children. Specific probiotic bacteria may promote potentially anti-allergenic processes through induction of Th1-type immunity and enhance the regulatory lymphocyte. This study investigated whether orally administrated probiotics could suppress allergic responses in an ovalbumin (OVA)-induced allergy mouse model. Thus, female C3H/HeJ mice were orally sensitized with OVA and cholera toxin for 4 weeks. Lactobacillus acidophilus AD031, Bifidobacterium lactis AD011, and L. acidophilus AD031 plus B. lactis AD011 were fed to mice from 2 weeks before the sensitization. The OVA-induced mice that were not treated with probiotics had significantly increased serum levels of OVA-specific IgE and IgG1, and OVA-specific IgA in feces. However, the mice treated with probiotics suppressed production of the OVA-specific IgE, IgG1, and IgA. The level of IL-4 was significantly lower, and the levels of INF-$\gamma$ and IL-10 were significantly higher in the mice treated with probiotics than that in the non-treated mice. The groups treated with probiotics had decreased levels of degranulated mast cells, eosinophil granules, and tail scabs. These results indicate that L. acidophilus AD031 and B. lactis AD011 might be useful for the prevention of allergy.

• Restorative Dentistry and Endodontics
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• 제17권1호
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• pp.222-234
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• 1992

The purpose of this study was to evaluate the systemic and local production of immunoglobulins and their levels in patients with periapical cysts using Enzyme - Linked Immunosorbent Assay. Streptococcus sanguis, Bacteroides gingivalis, and Bacteroides intermedius were grown for use as antigen and they were harvested by centrifugation. The patients were divided into two groups: patients of periapical cysts and normal control. 5 patients of each group were selected and their blood were obtained via intravenous puncture prior to surgical operation. Sera were prepared by centrifugation of each blood samples. Cyst fluid were aspirated from cystic cavity and cyst wall were excised at operation. Control tissue were also excised at extraction site of impacted wisdom teeth from normal control. Each tissue was prepared by homogenization and centrifugation. Then antibodies of each sample were measured by modified ELISA. The following results were obtained: 1. Serum IgG and IgM levels were not significantly different between patients with periapical cyst and normal control. 2. IgG and IgM levels of cyst fluid to Bacteroides gingivalis and Bacteroides intermedius were significantly higher than those of serum of patients with periapical cyst, but there was no significant difference to Streptococcus sanguis. 3. IgG and IgM levels of cyst wall to Bacteroides gingivalis and Bacteroides intermedius were significantly higher than those of control tissue, but there was no significant difference to Streptococcus sanguis. 4. IgG and IgM levels in cyst fluid and IgG levels in cyst wall were highest to Bacteroides gingivalis, and IgM levels in cyst wall were highest to Bacteroides intermedius.

• 한국식품영양학회지
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• 제8권2호
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• pp.70-78
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• 1995

We had examined the levels of specific IgG and IgA to dietary antigens in human breast milk and the relationships between the maternal food intake and the specific antibody level. The highest antibody titers were found in colostrum and decreased as lactation progressed. The specific antibody level was not affected by maternal calorie or protein intake, but affected by the intake frequency of a kind of food. Egg and meat intake significantly related to anti-OVA IgG and anti-BSA IgA antibodies, respectively. Meat intake frequency was generally affected by the other specific antibody levels.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.81-89
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• 2004

Tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and lymphotoxin-$\alpha$ (LT-$\alpha$, TNF-$\beta$) can initiate and perpetuate human diseases such as multiple sclerosis (MS), rheumatoid arthritis (RA), and insulin-dependent diabetes mellitus (IDDM). TNFs can be blocked by the use of soluble TNF receptors. However, since monomeric soluble receptors generally exhibit low affinity or function as agonists, the use of monomeric soluble receptors has been limited in the case of cytokines such as TNF-$\alpha$, TNF-$\alpha$, interleukin (IL)-1, IL-4, IL-6, and IL-13, which have adapted to a multi component receptor system. For these reasons, very high-affinity inhibitors were created for the purpose of a TNFs antagonist to bind the TNFR and trigger cellular signal by using the multistep polymerase chain reaction method. First, recombinant simple TNFR-Ig fusion proteins were constructed from the cDNA sequences encoding the extracellular domain of the human p55 TNFR (CD120a) and the human p75 TNFR (CD120b), which were linked to hinge and constant regions of human $IgG_1$ heavy chain, respectively using complementary primers (CP) encoding the complementary sequences. Then, concatameric TNFR-Ig fusion proteins were constructed using recombinant PCR and a complementary primer base of recombinant simple TNFR-Ig fusion proteins. For high level expression of recombinant fusion proteins, Chinese hamster ovary (CHO) cells were used with a retroviral expression system. The transfected cells produced the simple concatameric TNFR-Ig fusion proteins capable of binding TNF and inactivating it. These soluble versions of simple concantameric TNFR-Ig fusion proteins gave rise to multiple forms such as simple dimers and concatameric homodimers. Simple TNFR-1g fusion proteins were shown to have much more reduced TNF inhibitory activity than concatameric TNFR-Ig fusion proteins. Concatameric TNFR-Ig fusion proteins showed higher affinity than simple TNFR-Ig fusion proteins in a receptor inhibitor binding assay (RIBA). Additionally, concatameric TNFR-Ig fusion proteins were shown to have a progressive effect as a TNF inhibitor compared to the simple TNFR-Ig fusion proteins and conventional TNFR-Fc in cytotoxicity assays, and showed the same results for collagen induced arthritis (CIA) in mice in vivo.

• Agricultural and Biosystems Engineering
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• 제5권1호
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• pp.21-24
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• 2004

Antibody based biosensors are very selective and ultra-sensitive. Antigen-antibody reactions have been used in immunoassays. In this research, a biosensor which uses antigen-antibody reaction was developed to measure and detect rat IgG. Because the antigen-antibody reaction is a physical bounding between antigen and antibody, there are several ways to measure an antigen-antibody reaction. Among the methods, impedance analysis has short measuring time and possibilities of analyzing various properties of the reaction using frequency analysis. Rat IgG could be detected with developed biosensor and impedance analyzer. The biosensor showed good repeatability and availability of detecting concentration changes of rat IgG.

• 대한핵의학회지
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• 제29권1호
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• pp.92-97
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• 1995

감염병소와 진단을 위해 여러 방사성성핵종 표지 사람비특이항체들이 임상이용되었으나, $^{123}I,\;^{99m}Tc,\;^{111}In$등 표지 핵종과 표지방법에 따른 체내동태의 차이에 대한 자료가 필요하며, 감염병소의 진단에 표준적으로 이용되어지던 $^{111}In$-oxine표지백혈구와 비교평가도 요구된다. 저자들은 $10^9$개의 포도상구균을 좌측 대퇴부에 주사하여 농양을 유발한 백서에서 $^{123}In$ 표지, iminothiolane을 이용한 $^{99m}Tc$ 표지, DTPA이용 $^{111}In$ 표지 사람비특이항체와 $^{111}In$-oxine 표지 백혈구의 체내동태 및 농양섭취율을 비교하였다. $^{123}In$-IgG는 갑상선 및 위의 방사능이 높아 체내 탈요드반응이 빠름이 시사되었으며, $^{99m}Tc$-iminothiolane IgG는 신장방사능이 높아 신장으로 IgG 또는 대사물이 배설됨을 알 수 있었다. $^{111}In$-oxine표지 백혈구는 간 및 비장의 방사능이 높았고, 혈액방사능 제거율이 가장 빨랐다. 주사 24시간 후의 농양섭취율은 $^{111}In$-DTPA IgG가 가장 높았고, 농양 대 혈액 방사능비는 $^{111}In$-oxine표지 백혈구가 가장 높았으며, $^{111}In$-DTPA IgG와 $^{99m}Tc$-iminothiolane IgG가 다음으로 비슷하였다. $^{111}In$-oxine표지 백혈구보다는 방사성핵종표지 IgG가 간편하게 이용될 수 있으며, $^{111}In$ 이 $^{99m}Tc$나 $^{123}In$보다 지연영상의 촬영에 유리함을 알 수 있었다.