• Parasites, Hosts and Diseases
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• v.46 no.3
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• pp.187-189
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• 2008

House dust mites produce inhalant allergens of importance to allergic patients, We measured the major group 1 allergens, Der p 1 and Der f 1, from the house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farina, respectively in 100 randomly selected domestic homes from Cheonan, Korea, Dust samples were collected by vacuuming from the living room floor and 1 mattress in each home, Der p 1 and Der f 1 were measured by double mono-clonal ELISA. Der p 1 levels were very low, with geometric mean levels for floors and mattresses being $0.11\;{\mu}g/g$ (range: 0.01-4.05) and $0.14\;{\mu}g/g$ (range: 0.01-30.0), respectively. Corresponding levels of Der f 1 were higher, $7.46\;{\mu}g/g$ (range: 0.01-262.9) and $10.2\;{\mu}g/g$ (range: 0.01-230.9) for floors and mattresses, respectively. D. farinae appears to be the dominant house dust mite in Cheonan.

• Biomedical Science Letters
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• v.21 no.3
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• pp.152-159
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• 2015

Der p 2, which is a major allergen of house dust mite, plays an important role in the pathogenesis of allergic disease. There is controversy regarding whether Der p 2 binds to Toll-like receptor 4 (TLR4), and its inflammatory effect has not yet been elucidated. In the current study, we examined the interaction of Der p 2 with TLR4 and the effect of Der p 2 on cytokine release in THP-1 cells and lymphocytes. Among house dust mite extracts, recombinant TLR4 protein interacted with Der p 2. The overall structure of Der p 2 is characteristic of the immunoglobulin superfamily and contains ten ${\beta}-strands$, forming a ${\beta}-cup$ fold with two anti-parallel ${\beta}-sheets$, and a short 310 helix. The two sheets can be separated, further allowing the formation of a large internal pocket, which is narrow and suitable for binding large flat molecules such as lipid-like molecules. Der p 2 caused increased secretion of IL-6, IL-8, and MCP-1, which are neutrophil survival factors, in human monocytic THP-1 cells in a time-dependent manner. Der p 2 also induces the release of cytokines in normal and allergic lymphocytes. Supernatant after treatment with Der p 2 inhibited neutrophil apoptosis. In coculture of lymphocytes with neutrophils, Der p 2 inhibited spontaneous apoptosis of allergic neutrophils. In summary, Der p 2 binds to TLR4 and induces an inflammatory response such as cytokine secretion in immune cells. These findings may enable elucidation of allergy pathogenesis by specific allergen of house dust mite.

• Parasites, Hosts and Diseases
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• v.47 no.4
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• pp.387-391
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• 2009

Specific IgE against Acarus siro, Glycphagus domesticus, Tyrophagus putrescentiae, and Lepidoglyphus destructor have been investigated by ELISA in sera of 92 children. Of them, 41 were found to be specific IgE positive ($\geq$ 0.35 IU/ml) against at least one of house dust mite species, Dermatophagoides pteronyssinus and Dermatophagoides farinae, by an immunoblot. In 65.9% of the dust mite-sensitized children, specific IgE against at least one of these mite species was found. Sensitization levels, including co-sensitization cases were found to be 35.7% against A. siro, 24.4% against T. putrescentiae, 31.7% against L. destructor, and 26.8% against G. domesticus. In non-sensitized children, dust mite sensitization level was found to be 25.5%. Breakdown of sensitization by individual species in this group was; against A. siro and T. putrescentiae at 7.8%, against L. destructor at 13.7%, and against G. domesticus at 9.8%. When all children were reckoned, 43.5% was found to be sensitized against at least one storage mite species, with sensitizations against A. siro at 18.5%, T. putrescentiae at 26.1%, L. destructor at 21.7%, and G. domesticus at 17.4%. In dust samples collected from the dwellings of children, distribution of species was found to be A. siro (17%), G. domesticus (23%), T. putrescentiae (29%), L. destructor (25%), and unidentified (6%). In Fisher's chi-square test on SPSS program, there was a relationship between dust mite sensitization and storage mite sensitization (P < 0.05), but no meaningful relationship was found on the basis of individual mite species.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.4
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• pp.749-752
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• 2006

The house dust mites are well known to the most important causative allergens of major allergic diseases like pediatric asthma, allergic rhinitis or atopic dermatitis. This study was done for assessment of naphthalene effect against breeding suppression of the house dust mites. Twenty live adult house dust mites(Dermatophaogoides pteronyssinus) were each inoculated on mixed culture media containing 0 mg (control), 1mg, 2 mg, 3 mg, 5 mg, and 10 mg naphthalene and incubated at $25^{\circ}C$ with a relative humidity of 75%. After 4 weeks mean number of live house dust mites were 191.5, 24.3, 1.3, 1.3, 0, and 0, respectively. Above results showing that the naphthalene can suppress of breeding the house dust mites in vitro.

• Parasites, Hosts and Diseases
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• v.44 no.1 s.137
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• pp.73-79
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• 2006

We evaluated the efficacy of health education in reducing indoor arthropod allergens in Seoul. The mite control measures comprised the use of mite-proof mattress and pillow coverings, regular washing of potentially infested materials, maintenance of a low humidity, removal of carpets, and frequent vacuum cleaning. Cockroach control measures included trapping, application of insecticides, and protecting food. Of 201 homes enrolled in October 1999, 63 volunteers were included in a 2-year follow-up survey between April 2000 and January 2002. Before intervention, the density of mites/g of dust varied greatly; 27.1/g in children's bedding, 20/g in adult bedding, 7.2/g on the floors of children's bedrooms, 6.8/g in sofas, 5.9/g on the floors of adult's bedrooms, 3.9/g on living room floors, 3.7/g in carpets, and 1.9 mites/g on kitchen floors. The predominant mite species and house percentages infested were; Dermatophagoides farinae $93\%$, D. pteronyssinus $9\%$, and Tyrophagus putrescentiae $8\%$. Comparing 1999 and 2001 infestations, before and after 25 mo of education, mite abundance was reduced by $98\%$, from 23.7 to 0.57 mites/g of dust. In 1999, cockroaches were detected in $62\%$ homes: $36\%$ Blattella germanica and $35\%$ Periplaneta spp., including $9\%$ double infestations of B. germanica and P. americana. Following intervention, cockroach infestation rates decreased to $22\%$ of houses in 2000 and $23\%$ in 2001. We conclude that continuous and repetitive health education resulted in the effective control of domestic arthropods.

• Journal of Korean Public Health Nursing
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• v.10 no.2
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• pp.51-60
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• 1996

This study was conducted to provide basic knowledges of effective environmental control of patients with allergic rhinitis. From July to October 1995, 58 dust samples Were drawn from the bedrooms of patients with allergic rhinitis who were registerd at an allergy clinic of a hospital. Those samples were examined for the amount of house mite allergens & dusts. The data were analysed by using ANOVA and Pearson correiation coefficients. The results were as follows : 1. As for the amount of house mite allergens in terms of environmental factors, the amount of house mite allergens of using washing water temperature of bedding above than $55^{\circ}C$ was significantly lower than that of below $54^{\circ}C$. Other environmental factors such as type of house, area of bed room floor, bed in bedroom, bedroom cleaning by vacuum cleaner, days after bedding washing, relative humidity of bedroom were relate to the amount of house mite allergens. 2. As for the amount of dusts in terms of environmental factors, the amount of dusts of days of days after bedding washing more than 15 days was significantly lower than thant of less than 15days. Other environmental factors such as type of house, area of bedroom floor, bed in bedroom, bedroom cleaning by vacuum cleaner, was hing water taemperature of bedding, relative humidity of bedroom were not relate to the amount of dusts. 3. There was a significant positive correlation between the amount of house mite allergens and the amount of dusts. It may be conclusively said. the amount of dusts and house mite allergens were closely associated with the washing temperature and days after washing of bedding. Hence. intensive instruction for the methods of bedding washing was needs of the patients with allergic rhinitis.

• Parasites, Hosts and Diseases
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• v.35 no.1
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• pp.9-17
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• 1997

During the period of 1933-1994, house dusts were collected from 65 homes at 10 different localities by operating electric vacuum cleaners. House dust mites were isolated from 10 g dust by applying the modified wet sieving method. Total 7,257 mites were collected and 23 species were identified. Among them, Demctophqgoines loyinaf (DF) was predominant (65. Soy) of the total) , followed by D. pteronvssinus (DP) (20.6%) and Tyrophagus nutrescentios (TP) (6.5%) . Rhizoglvphur robini, Sancqsscnin phvIlophogiann, Cheyletus trcussarti and Scheloribates latipes were the first findings from Korea. DF was predominant in Seoul (66.8%), Kwangju (63.6%), inland of Pusan (79.6%1, Inchon (96.5%), Taejon (83.9%), Chouju (87.15) and Chongiu (95.2%), whereas DP was predominant in Yongkwang-ub (72.5%) and Yongdo (island) of Pusan (64.9%), and TP in Chunchon (38.2%) The localities where DP and TP were predominant showed higher relative humidity in air (> 73% RH) . Among 62 study homes, DF, DP and TP were found in 24.6% of the homes, co-habitat of two species in 48.1% and one species in 27.3%. DF was predominant in 63.5% of the homes studied, DP in 29.6% and TP in 6.9%. In 10 g of the house dust, less than 99 mites were found in 49 homes (70.0%), 100-499 mites in 11 homes (15.7%), 500-999 mites in 3 homes (4.3%) and more than 1,000 mites in 2 homes (2.9%). No mite was found in 5 homes (7.1%). In order to evaluate environmental factors affecting the population density of house dust mites, house type, age of house construction, size of the house, number of the family and frequency of the cleaning were compared with the number of mites, and none of the above factors were statistically correlated with the mite density.

• Proceedings of the Korea Contents Association Conference
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• 2014.06a
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• pp.327-328
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• 2014

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.54-54
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• 2005

• The Journal of Pediatrics of Korean Medicine
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• v.30 no.4
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• pp.77-86
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• 2016

Objectives Hataedock (HTD) is an oral Korean herbal medical oral treatment that removes fetal toxic heat and meconium from new born babies. The purpose of this study is to evaluate whether Hataedock treatment of Duchi extracts has anti-inflammation effects in atopic dermatitis-like skin lesions in House Dust Mite-Induced NC/Nga Mice. Methods The mice were divided into 3 groups (n=10 per group) as follows: the control group (Ctrl group), AD-induced group (AE group), AD-induced with HTD treatment group (DT group). 3-week-old NC/Nga mice were introduced to Hataedock treatment, made of Duchi extract. After 4 weeks, House Dust Mite-Induced application was used six times per week for 3 weeks to induce the first atopic dermatitis, and second AD in 7 weeks after. To examine skin injuries and anti-inflammatory effect, PKC, MMP-9, iNOS immunohistochemistry were used. Results The alleviate effect of the skin damage and angiogenesis was observed in DT group. The damage of stratum corneum, hyperplasia, edema, infiltration of lymphocytes and distribution of capillary were decreased in DT group. Also, the study results suggested that Hataedock treatment made of Duchi extracts in DT group remarkably decreased skin damages by 51% (p < 0.001), as well as PKC by 91%, MMP-9 by 48% (p < 0.001), iNOS by 51% (p < 0.001). Conclusions Based on the study results, we observed that Hataedock treatment of Duchi extracts alleviates AD by diminishing various inflammatory cytokines, initial steps of AD development, in the skin lesions. Potential applications for prevention and treatment of atopic dermatitis are expected.