• Title/Summary/Keyword: house detection

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Genetic Study of Soybean Sudden Death Syndrome Pathogen(Fusarium solani f. sp. glycines) isolated from Geographically Different Fields based on RFLPs of Mitochondrial DNA

  • Cho, Joon-Hyeong;J. C. Rupe
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.2
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    • pp.143-149
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    • 2000
  • From the soils of soybean fields in Cotton Branch Station (CBS) and Pine Tree Station (PTS), Arkansas, USA, various single spore isloates of sudden death syndrome (SDS) pathogen were obtained on modified Nash & Snyder's medium (MNSM) with dilution plating technique and transferred to potato dextrose agar (PDA) medium to identify the cultural colony shape. The colony shapes of these isolates resembled F. solani isolate 171 which was white and chalky shaped on MNSM and most of them had unique form of morphology which produced white margin and blue center colony on PDA. Although, some of these isolates had more dark blue or showed slightly different color, all isolates that were selected randomly for green-house inoculation assay produced typical foliar symptoms on leaves of soybean, Hartz 6686. To determine the genetic differences among the isolates, mitochondrial DNA restriction fragment length polymorphism (RFLP) was conducted with fourty isolates from both fields, using mtDNA probes, 2U18 and 4U40, derived from Colletotrichum orbiculare. We obtained distinctive RFLPs in each treatment of restriction enzyme, EcoRI and HaeⅢ. Isolates, 11-2-5 and 14-3-1-1, from CBS and isolates, 104-3-1-2 and 701-1-5-1, from PTS showed different band patterns from 171 in both or in either treatment of restriction enzymes. Even if some of these isolates showed heterogeneous, they were more closer to 171 than PN603. And, also, rest of the thirty-six isolates had exactly same polymorphisms as 171 in each treatment of restriction enzyme. Although, some of the isolates showed the different morphological shape on PDA and slightly different band patterns on RFLPs, all of the isolates selected on MNSM due to their distinctive colony shape from other fungi produced the typical foliar symptoms on soybean leaves in greenhouse inoculation assay. It might be suggested that these isolates were not genetically different from check isolate 171 and they were unique strain of F. solani.

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Detection of Waterborne Pathogens in Public Bath Houses by PCR-Reverse Blot Hybridization Assay (PCR-REBA) (분자생물학적 방법인 PCR-REBA를 이용한 대중목욕탕 수질 중 수인성병원성미생물 검출)

  • Song, Woon-Heung;Choi, Seung-Gu;Yang, Byoung-Seon;Lee, Jae-Sang
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.8
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    • pp.3517-3522
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    • 2011
  • Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to background rates of disease. The aim of this study is to determine the prevalence of waterborne pathogens in public baths. A total of 30 water samples were collected from 30 different public baths in seoul, Korea. Pathogens in water samples were concentrated by 0.45 ${\mu}m$ nitrocellulose membrane filter, analyzed by both cultivation and polymerase chain reaction-reverse blot hybridization (PCR-REBA) of partial 16S rRNA gene. Various microorganisms including Escherichia coli and Shigella spp. were identified by microbiological cultivation. E. coli, Shigella spp., Salmonella spp., Pseudomonas spp. and Mycobacterium spp. were identified by PCR-REBA. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.

Detection of Genital HPV Infection Using Urine Samples: a Population Based Study in India

  • Sabeena, Sasidharanpillai;Bhat, Parvati;Kamath, Veena;Mathew, Mary;Aswathyraj, Sushama;Devadiga, Santhosha;Prabhu, Suresha;Hindol, Maity;Chameetachal, Akhil;Krishnan, Anjana;Arunkumar, Govindakarnavar
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.3
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    • pp.1083-1088
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    • 2016
  • Background: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. Objective: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years. Materials and Methods: The study area chosen was Perdoor village in Udupi Taluk, Karnataka State and all the women in the age group of 18-65 years formed the study cohort. A cross sectional study was conducted by house visits and 1,305 women were enrolled in the study. After taking written informed consent a data sheet was filled and early stream random urine samples were collected, transported to a laboratory at 4OC and aliquoted. Samples were tested using nested HPV PCR with PGMY09/11 and GP5+/6+ primers. Positive cases were genotyped by sequence analysis. Results: Study participants included 1,134 sexually active and 171 unmarried women with a mean age at marriage of 22.1 (SD=3.9) years. Study area showed high female literacy rate of 86.6%. Five urine samples tested positive for HPV DNA (0.4%). Conclusions: We found very low genital HPV infection rate among women from monogamous community. This is the first major population based study carried out among asymptomatic rural women to detect genital HPV infectio from Karnataka using urine samples.

Domestic Efforts for SFCL Application and Hybrid SFCL (국내 초전도 한류기 요구와 하이브리드 초전도 한류기)

  • Hyun, O.B.;Kim, H.R.;Yim, Y.S.;Sim, J.;Park, K.B.;Oh, I.S.
    • Progress in Superconductivity
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    • v.10 no.1
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    • pp.60-67
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    • 2008
  • We present domestic efforts for superconducting fault current limiter (SFCL) application in the Korea Electric Power Corporation (KEPCO) grid and pending points at issue. KEPCO's decision to upgrade the 154 kV/22.9 kV main transformer from 60 MVA to 100 MVA cast a problem of high fault current in the 22.9 kV distribution lines. The grid planners supported adopting an SFCL to control the fault current. This environment friendly to SFCL application must be highly dependent upon the successful development of SFCL having specifications that domestic utility required. The required conditions are (1) small size of not greater than twice of 22.9 kV gas insulated switch-gear (GIS), (2) sustainability of current limitation without the line breaking by circuit breakers (CB) for maximum 1.5 seconds. Also, optionally, recommended is (3) the reclosing capability. Conventional resistive SFCLs do not meet (1) $\sim$ (3) all together. A hybrid SFCL is an excellent solution to meet the conditions. The hybrid SFCL consists of HTS SFCL components for fault detection and line commutation, a fast switch (FS) to break the primary path, and a limiter. This characteristic structure not only enables excellent current limiting performances and the reclosing capability, but also allows drastic reduction of HTS volume and small size of the cryostat, resulting in economic feasibility and compactness of the equipment. External current limiter also enables long term limitation since it is far less sensitive to heat generation than HTS. Semi-active operation is another advantage of the hybrid structure. We will discuss more pending points at issues such as maintenance-free long term operation, small size to accommodate the in-house substation, passive and active control, back-up plans, diagnosis, and so on.

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Prevalence of Toxoplasma gondii in Dogs in Zhanjiang, Southern China

  • Jiang, Hai-Hai;Li, Ming-Wei;Xu, Min-Jun;Cong, Wei;Zhu, Xing-Quan
    • Parasites, Hosts and Diseases
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    • v.53 no.4
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    • pp.493-496
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    • 2015
  • Toxoplasmosis, caused by Toxoplasma gondii, is a parasitic zoonosis with worldwide distribution. The present study investigated the prevalence of T. gondii in dogs in Zhanjiang city, southern China, using both serological and molecular detection. A total of 364 serum samples and 432 liver tissue samples were collected from the slaughter house between December 2012 and January 2013 and were examined for T. gondii IgG antibody by ELISA and T. gondii DNA by semi-nested PCR based on B1 gene, respectively. The overall seroprevalence of T. gondii IgG antibody was 51.9%, and T. gondii DNA was detected in 37 of 432 (8.6%) liver tissue samples. These positive DNA samples were analyzed by PCR-RFLP at 3'- and 5'-SAG2. Only 8 samples gave the PCR-RFLP data, and they were all classified as type I, which may suggest that the T. gondii isolates from dogs in Zhanjiang city may represent type I or type I variant. This study revealed the high prevalence of T. gondii infection in dogs in Zhanjiang city, southern China. Integrated measures should be taken to prevent and control toxoplasmosis in dogs in this area for public health concern.

Accuracy Assessment of 3D Reconstruction Using LiDAR Data (LiDAR 자료를 이용한 3차원복원 정확도 평가)

  • Chung, Dong-Ki
    • Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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    • 2005.11a
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    • pp.81-104
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    • 2005
  • Accurate 3D models in urban areas are essential for a variety of applications, such as virtual visualization, CIS, and mobile communications. LiDAR(Light Detection and Ranging) is a relatively new technology for directly obtaining 3D points. Because Manual 3D data reconstruction from LiDAR data is very costly and time consuming, many researchs is focused on the automatic extraction of the useful data. In this paper, we classified ground and non-ground points data from LiDAR data by using filtering, and we reconstructed the DTM(Digital Terrain Model) using ground points data, buildings using nonground points data. After the reconstruction, we assessed the accuracy of the DTM and buildings. As a result of, DTM from LiDAR data were 0.16m and 0.59m in high raised apartments areas and low house areas respectively, and buildings were matched with the accuracy of a l/5,000 digital map.

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Antituberculosis Medication in Children (소아의 항결핵제 사용 실태)

  • Kim, Mun Hee;Shin, Young Kyoo;Park, Sang Hee;Tockgo, Young Chang
    • Pediatric Infection and Vaccine
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    • v.3 no.2
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    • pp.128-132
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    • 1996
  • Tuberculosis in children is an important disease because of higher incidence and mortality, especially in developing and underdeveloped countries. The objectives of this study were to evaluate the cause of antituberculosis medication in children and to find out the basic data for proper drug regimen. We reviewed the medical records of 198 patients who had been treated with antituberculosis drugs from Jan. 1991 to Dec. 1993 in Anam Hospital of Korea University Medical Center. The results are as following; 1) Of 198 patients, 69 cases(34.8%) had treated due to BCG complications. They were all medicated with INH. The durations of medication were 3 months in 46 patients(66.7%), 4~6 months in 17 patients(5.8%), 7~9 months in 4 patients(5.8%), 10-12 months in 2 patients(2.9%). 2) Of 198 patients, 68 cases(34.3%) had treated due to chemoprophylaxis, 59 patients (29.8% of all cases) had histories of house hold contact. Of 68 cases, 51 patients (86.4%) were medicated with INH only, 8 patients (13.6%) were medicated with INH and RFP. 3) Other causes of antituberculosis medication were tuberculous lymphadenitis(14.1%), pulmonary tuberculosis(10.6%), meningitis, miliary tuberculosis(2.0%), and pleurisy(2.0%). Most common causes of antituberculosis medications in children were complication of BCG vaccination and chemoprophylaxis after household contact. So early detection of adult tuberculosis and development of convenient diagnostic methods and safe vaccine for childhood tuberculosis is necessary.

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Effects of loose farrowing facilities on reproductive performance in primiparous sows

  • Choi, Yohan;Min, Yejin;Kim, Younghwa;Jeong, Yongdae;Kim, Doowan;Kim, Joeun;Jung, Hyunjung
    • Journal of Animal Science and Technology
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    • v.62 no.2
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    • pp.218-226
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    • 2020
  • The present study investigated the effects of loose farrowing type during gestation and after farrowing on reproductive performance and of lactating sows. A total of 22 primiparous sows (Landrace; average initial body weights [BW], 228.54 ± 12.79 kg) were allotted to one of two treatments on the basis of body weight. Sows were divided into two experimental groups, conventional farrowing crates (CON), and loosed-farrowing pens (LFP). The experiment duration was around 38 days ranging from 10 days before parturition to 28 days after parturition. Gestating sows at the age of 105 d were placed in gestational stalls (group housing). All the sows were fed a common diet according to the National Research Council requirements for lactation. Cross-fostering was performed within 1 day of parturition. From 1 day after weaning, estrus detection was performed twice-daily (0900 and 1730 h) for 10 min by boar exposure. There were significant effects of LFP housing type on the farrowing duration, and farrowing interval. At the farrowing time, none of the litter parameters including total born, stillborn, mummy, born alive piglets and total litter weight and piglet weight were affected. There were no effects of housing type on the mortality of piglets at d 1, 3, 7, 21, and 28. In conclusion, the result of this study showed that there is no performance difference between the crated or LFP sows, which indicate that the LFP housing has the potential to be used as an alternative to the crated house without any detrimental effects in reproduction performance of lactating sows.

Monitoring of Pig Body Temperature Using Infrared Sensors (적외선 센서를 이용한 돼지 체온 모니터링)

  • Jang, Jin Cheol;Lee, Jun Yeop;Lee, Sang Yoon;Kim, Hyuck Joo;Choi, Dong Yoon;Lee, Sunghyoun;Kim, Hyeon Tae
    • Journal of Animal Environmental Science
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    • v.20 no.3
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    • pp.91-96
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    • 2014
  • The temperature of a pig is the most key point in determining, it's health status. We wanted to monitor the body temperature of pig to find out if any changes would occur, we used 3 minipigs (about 20 kg) who were feed using a feeding system while being confined in a pig house. The infrared testings were taken from a height of 30 cm above the pigs backs over a period of 28 days. We were able to conclude that the results between the back and indoor temperature were y = 0.5487x + 18.459. These values were compared with the values found after infrared sensor results were taken. We found an error range of $0.004{\sim}1.82^{\circ}C$ and an average of $0.58^{\circ}C$. In conclusion, using an infrared thermometer made monitoring of pigs back possible. This system seems to be feasible and effective in monitoring pig temperature.

Development of Magnetic Sensor for Live Line Detector of the Underground Cable (지중케이블 활선검출기를 위한 자장 센서 개발)

  • Kim, Ki-Joon;Oh, Yong-Cheul;Lee, Kyeong-Seob;Jung, Han-Seok;Kim, Tag-Yong;Choi, Mi-Hui;Soung, Min-Yeong;Shin, Cheol-Gi;Kim, Jin-Sa
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.24 no.2
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    • pp.166-171
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    • 2011
  • We use the electrical energy and it is essential energy in modern life, but we lay cable underground due to the issue for environment and safety. Safety for worker is still insufficient for the development of safety equipment and related research has been focused on the cable lifetime diagnosis at underground cable work. I have to develop live line detector around the magnetic field were investigated at underground cable. In this paper, we were investigated by variation of coil turns and load due to detection of magnetic field at lines around. And detected value of developing products compared with measured value of milli-gauss meter. As a result, the value of the number of coil turns was found to be proportional to the measured value. But turn-numbers increase showed that the weak noise. I could be confirmed that sensor showed the optimum value from 4,000 to 5,0000.