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http://dx.doi.org/10.5762/KAIS.2011.12.8.3517

Detection of Waterborne Pathogens in Public Bath Houses by PCR-Reverse Blot Hybridization Assay (PCR-REBA)  

Song, Woon-Heung (Dept. of Clinical Laboratory Science, Shinheung College)
Choi, Seung-Gu (Dept. of Clinical Laboratory Science, Shinheung College)
Yang, Byoung-Seon (Dept. of Clinical Pathology, Jinju Health College)
Lee, Jae-Sang (Dongarm Medical Institute)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.12, no.8, 2011 , pp. 3517-3522 More about this Journal
Abstract
Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to background rates of disease. The aim of this study is to determine the prevalence of waterborne pathogens in public baths. A total of 30 water samples were collected from 30 different public baths in seoul, Korea. Pathogens in water samples were concentrated by 0.45 ${\mu}m$ nitrocellulose membrane filter, analyzed by both cultivation and polymerase chain reaction-reverse blot hybridization (PCR-REBA) of partial 16S rRNA gene. Various microorganisms including Escherichia coli and Shigella spp. were identified by microbiological cultivation. E. coli, Shigella spp., Salmonella spp., Pseudomonas spp. and Mycobacterium spp. were identified by PCR-REBA. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.
Keywords
Public bath house; Waterborne pathogens Polymerase Chain Reaction-Reverse Blot Hybridization (PCR-REBA);
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