• IMMUNE NETWORK
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• 제8권4호
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• pp.107-123
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• 2008

It has now been well documented in a variety of models that T regulatory T cells (Treg cells) play a pivotal role in the maintenance of self-tolerance, T cell homeostasis, tumor, allergy, autoimmunity, allograft transplantation and control of microbial infection. Recently, Treg cell are isolated and can be expanded in vitro and in vivo, and their role is the subject of intensive investigation, particularly on the possible Treg cell therapy for various immune-mediated diseases. A growing body of evidence has demonstrated that Treg cells can prevent or even cure a wide range of diseases, including tumor, allergic and autoimmune diseases, transplant rejection, graft-versus-host disease. Currently, a large body of data in the literature has been emerging and provided evidence that clear understanding of Treg cell work will present definite opportunities for successful Treg cell immunotherapy for the treatment of a broad spectrum of diseases. In this Review, I briefly discuss the biology of Treg cells, and summarize efforts to exploit Treg cell therapy for autoimmune diseases. This article also explores recent observations on pharmaceutical agents that abrogate or enhance the function of Treg cells for manipulation of Treg cells for therapeutic purpose.

• Asian-Australasian Journal of Animal Sciences
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• 제7권3호
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• pp.427-434
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• 1994

The primordial germ cells (PGCs) were transfected in vitro and expressed the exogenous RSVLTR/${\beta}G2$ plasmid, suggesting thaI PGC is a possible vector for direct gene transfer into the germ line. Transfection efficiency of cell suspensions containing PGCs was 1.5% by liposome mediated DNA transfection. By microinjection of the transfected PGCs into the host germinal crescent, PGCs migrated via blood vessel to the future gonad and these transfected PGCs resulted in the RSVLTR/${\beta}G2$ expression in the gonad. The results from the seeding of PGCs on the chorioallantoic membrane were insufficient to test the hypothesis that PGCs can penetrate or invade the chorioallantoic membrane for transport via the circulatory system.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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• pp.18-21
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• 2007

Digital image watermarking is a technology used in copyrighting of digital images by embedding unremovable informations. In this paper, a pixel-domain look-up-table-based watermarking algorithm is presented. With this methodology, the watermark was embedded in the host image, but we did not observe any distortion at certain specific region of interest. This means the proposed method is preferred in case of satellite images. Then, the image manipulation tool which is called 'StirMark' will be used to perform many kinds of attacks such as rotation, scaling, filtering and compression on the watermarked image. Finally, the effectiveness of a watermarking technique in terms of 'robustness' and 'data integrity' criteria will be measured by calculating PSNR of watermark and watermarked image.

• 미생물학회지
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• 제27권2호
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• pp.92-97
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• 1989

the vector, pGUR19, for Rhizobium gene manipulation, was constructed by combining the replication and mobilization function of indigenous multicopy plasmid from Acacia(Robinia pseudoacacia L.) Rhizobia sp86 with E. coli cloning vehicle, pBR322. The vector could be efficiently mobilized by RP4 tra function incorporated into chromosome of E. coli named SM10 and efficiently transferred to various gram negative hosts including Rhizobium and Afrobacterium by transformation. Mobilization frequency of the constructed vector was ranged from $1.2\times 10^{-2}$ (E.coli HB 101) to $4.6\times 10^{-4}$ (A. tumefaciens 15955) and transformation frequency was ranged from $5.4\times 10^{-7}$(E. coli HB101) to $1.2\times 10^{-10}$ (A. tumefaciens 15955). The vector, pGUR19, was stably replicated and maintained in a variety of Rhizobium and Agrobacterium.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2018년도 춘계학술대회 및 임시총회
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• pp.51-51
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• 2018

The application of recombinant DNA technology has been remarkable and nearly replaced commonly used traditional methods. Traditional industrial microbiology long depended on the discovery of valuable strains and mutagenesis of such strains to improve its secretion capacity of enzymes and secondary metabolites on the industrial scale. Commodities included industrial enzymes and biopharmaceuticals. The purpose of genome manipulation by the crossing of different strains or genetic recombination of naked DNA to the genome is of increased production of valuable metabolites. We optimized a transformation method to either for removal of innate genes, introduction of heterologous genes, or combination of both. We have been used selected whole or partial genes to manipulate target fungi toward the development of strains overproducing invaluable proteins. We have also used the whole genome sequence information of fungal genomes in public databases and functional genomics approach to select genes to manipulate and eventually contributing greatly to the development of overproducing industrial strains overproducing proteins or secondary metabolites. I will briefly review 1) filamentous fungi as a host for production of recombinant proteins and secondary metabolites, 2) markets of industrial metabolites, 3) a new approach to manipulate up to five genes at the same time in the system that ProxEnrem uses.

• 한국수정란이식학회지
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• 제29권1호
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• pp.83-90
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• 2014

The main purpose of this study is to estimate the effect of adding Tea-N-Tris (TES) to the freezing buffer for miniature pig sperm. In particular, we attempted to identify the association between the MMPs expression and the fertility and viability of frozen sperm from each extender (LEY (Lactose Egg-Yolk), TLE (TES + LEY), TFGE (TES + Fructose + Glucose Egg-Yolk)). In accordance with this, Hypoosmotic Swelling Test (HOST) respond test was the lowest among sperms frozen in LEY while the highest HOST respond was observed among sperms frozen in TLE. Furthermore, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of MMP-9 and MMP-2 was the highest in sperms frozen in LEY, Meanwhile, sperms from the TFGE and TLE group showed lower level of MMP-9 and MMP-2 expression in the order of TLE being the lowest. LEY group showed lower rate of blastocyst development than the TES supplement group, although the difference was not statistically significant. Meanwhile the rate of blastocyst development appeared similar when sperms from TLE and TFGE group were used for IVF. Together, these results indicate that adding Tea-N-Tris to the sperm freezing buffer only suppresses MMPs protein activation but also maximize in-vitro fertility, providing a means to improve the success rate in the in vitro manipulation of miniature pig sperm.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2003년도 추계종합학술대회
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• pp.320-324
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• 2003

최근, 가상현실분야가 매우 다양하게 각 산업분야에서 응용되고 있다. 본 논문에서는 가상환경하에서 인터페이스 조작에 의하여 로봇의 움직임을 제어하였다. 3D Graphic Tool을 사용하여 가상로봇을 생성하고 생성된 가상로봇에 실제로봇의 텍스쳐를 입혀 X파일로 변환시켜 3차원 가상현실에 적용되는 Direct 3D Graphic의 Component들을 사용하여 실제로봇과 동일한 모습을 재현하였다. 또한 조이스틱을 통해서 가상로봇의 움직임을 표현하고 실제로봇을 제어했다. 개발된 로봇은 크게 로봇 제어부와 Host PC에서의 Visual I/F program으로 구성되어 있다. 개발된 로봇의 구동부와 Camera의 구동부는 2자유도를 갖고 있으며 사용자 친화적으로 개발된 조이스틱으로 원격조종된다. 외부 상황은 비젼 시스템과 초음파 센서로 인식되며, 화상 및 센서 데이터, 명령어 둥을 각각 900MHz와 447MHz RF로 통신된다. 사용자는 실제 로봇을 제어하기 위하여 시뮬레이터를 이용하여 로봇 제어 명령을 내리면 로봇을 구동하기 위한 원격 송/수신부에서 447MHz의 특정소출력국용 주파수를 사용하는 모듈을 통해 Half duplex 방식으로 4800bps로 실외 500m까지 제어한다.

• International Journal of Computer Science & Network Security
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• 제21권1호
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• pp.143-151
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• 2021

The enormous prevalence of transferring official confidential digital documents via the Internet shows the urgent need to deliver confidential messages to the recipient without letting any unauthorized person to know contents of the secret messages or detect there existence . Several Steganography techniques such as the least significant Bit (LSB), Secure Cover Selection (SCS), Discrete Cosine Transform (DCT) and Palette Based (PB) were applied to prevent any intruder from analyzing and getting the secret transferred message. The utilized steganography methods should defiance the challenges of Steganalysis techniques in term of analysis and detection. This paper presents a novel and robust framework for color image steganography that combines Linear Congruential Generator (LCG), simulated annealing (SA), Cesar cryptography and LSB substitution method in one system in order to reduce the objection of Steganalysis and deliver data securely to their destination. SA with the support of LCG finds out the optimal minimum sniffing path inside a cover color image (RGB) then the confidential message will be encrypt and embedded within the RGB image path as a host medium by using Cesar and LSB procedures. Embedding and extraction processes of secret message require a common knowledge between sender and receiver; that knowledge are represented by SA initialization parameters, LCG seed, Cesar key agreement and secret message length. Steganalysis intruder will not understand or detect the secret message inside the host image without the correct knowledge about the manipulation process. The constructed system satisfies the main requirements of image steganography in term of robustness against confidential message extraction, high quality visual appearance, little mean square error (MSE) and high peak signal noise ratio (PSNR).

• 한국미생물·생명공학회지
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• 제23권2호
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• pp.164-169
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• 1995

An endogenous cryptic plasmid, pBL1, which has been used to construct plasmid vectors for coryneform bacteria producing amino acids, was eliminated from Brevibacterium lactofermentum. The pBL1 was partially digested with Sau3AI and the resulting DNA fragments were subcloned into a suicide vector pEM1 which contains a kanamycin-resistant (km$^{r}$) gene. KM$^{r}$ B. lactofermentum transconjugants were obtained by conjugal transfer of the pEM1 derivatives containing pBL1 DNA fragments from Escherichia coli into B. lactofermentum. A km$^{r}$ transconjugant was analyzed to contain a plasmid pEB14, which occurred in vivo by homologous recombination between pBL1 and the conjugal-transferred plasmid. The pEB14 including the pEM1-derived km$^{r}$ gene was found to be lost concomitantly with km$^{r}$ phenotype, resulting in the construction of a pBL1-free strain of B lactofermentum. Based on transformation efficiencies and plasmid stability, the resultant pBL1- free strain is more useful than wild strain as a host cell for genetic manipulation. It could be concluded that foreign plasmid DNAs are efficiently isolated and analyzed from the pBL1-free strain because of the absence of endogenous pBL1 plasmid.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권4호
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• pp.219-226
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• 2000

The application of recombinant DNA technology to restructure metabolic net-work can change metabolite and protein products by altering the biosynthetic pathways in an organism. Although some success has been achieved, a more detailed and thorough investigation of this approach is certainly warranted since it is clear that such methods hold great potential based on the encouraging results obtained so far. In last decade, there have been tremendous advances in the field of glycobiology and the stage has been set for the biotechnological production of glycoproteins for therapeutic use. Today glycoproteins are one of the most important groups of pharmaceutical products. In this study the attempt was made to focus on identifying technologies that may have general application for modifying glycosylation pathway of the yeast cells in order to produce glycoproteins of therapeutic use. The carbohydrates of therapeutic recombinant glycoproteins play very important roles in determining their pharmacokinetic properties. A number of biological interactions and biological functions mediated by glycans are also being targeted for therapeutic manipulation in vivo. For a commercially viable production of therapeutic glycoproteins a metabolic engineering of a host cell is yet to be established.