• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2008년도 추계종합학술대회 B
• /
• pp.66-69
• /
• 2008

IP계층에서의 이동성 지원에 대한 연구가 활발히 진행되어 IETF에서 MIPv6에 대한 표준화가 진행되었다. MIPv6는 이동 노드가 망과 망 사이를 이동할 때, 서비스에 대한 연결성을 유지하여 끊김 없는 통신을 가능하게 해주며, 이러한 방법을 핸드오버라고 한다. IEEE 802.21에서는 MIH(Media Independent Handover)라는 끊김 없는 핸드오버를 위한 방법을 표준화했다. MIH는 하위 레이어와 상위 레이어에서의 핸드오버를 위해 세 가지 서비스를 사용하였으며, 본 논문에서는 기존의 MIH에 두 가지 모드를 적용하여 네트워크 시뮬레이터에서 듀얼 모바일 호스트와 싱글 모바일 호스트의 성능 평가를 수행하였다.

• 한국어병학회지
• /
• 제34권1호
• /
• pp.17-22
• /
• 2021

Eukaryotic translation is initiated by either cap-dependent or cap-independent way, and the cap-independent translation can be initiated by the internal ribosomal entry site (IRES). In this study, to know whether the 5'UTR leader sequence of marine birnavirus (MABV) segment A and segment B can act as IRES, bicistronic vectors harboring a CMV promoter-driven red fluorescent gene (mCherry) and poliovirus IRES- or MABV's leader sequence-driven green fluorescent gene (eGFP) were constructed, then, transfected into a mammalian cell line (BHK-21 cells) and a fish cell line (CHSE-214 cells). The results showed that the poliovirus IRES worked well in BHK-21 cells, but did not work in CHSE-214 cells. In the evaluation of MABV's leader sequences, the reporter eGFP gene under the 5'UTR leader sequence of MABV's segment A was well-translated in CHSE-214 cells, indicating 5'UTR of MABV's segment A initiates translation in the cap-independent way and can be used as a fish-specific IRES system. However, the 5'UTR leader sequence of MABV's segment B did not initiate translation in CHSE-214 cells. As the precise mechanism of birnavirid IRES-mediated translation is not known, more elaborate investigations are needed to uncover why the leader sequence of segment B could not initiate translation in the present study. In addition, further studies on the host species range of MABV's segment A IRES and on the screening of other fish-specific IRESs are needed.

• 무역상무연구
• /
• 제44권
• /
• pp.239-255
• /
• 2009

The purpose of this article is to examine the Utilization of Umbrella Clauses in Investor-State Contract Disputes. To accomplish the purpose, this article analyzes the ICSID case of SGS v. Pakistan and SGS v. Philippines. Umbrella clauses have become a regular feature of international investment agreements and have been included to provide additional protection to investors by covering the contractual obligations in investment agreements between host countries and foreign investors. In particular, two recent ICSID decisions, SGS v. Pakistan and SGS v. Philippines, have brought to the forefront the question of whether the umbrella clause applies to obligations arising under otherwise independent investment contracts between the investor and the host State. In focusing on the SGS decisions, this article will give some useful guidelines to Government and Academia under currently prevailing environment of the Free Trade Agreement("FTA") in Korea.

• 음성과학
• /
• 제15권2호
• /
• pp.67-77
• /
• 2008

In this paper, we implemented a robust speech recognizer using the TMS320VC33 DSP. For this implementation, we had built speech and noise database suitable for the recognizer using spectral subtraction method for noise removal. The recognizer has an explicit structure in aspect that a speech signal is enhanced through spectral subtraction before endpoints detection and feature extraction. This helps make the operation of the recognizer clear and build HMM models which give minimum model-mismatch. Since the recognizer was developed for the purpose of controlling car facilities and voice dialing, it has two recognition engines, speaker independent one for controlling car facilities and speaker dependent one for voice dialing. We adopted a conventional DTW algorithm for the latter and a continuous HMM for the former. Though various off-line recognition test, we made a selection of optimal conditions of several recognition parameters for a resource-limited embedded recognizer, which led to HMM models of the three mixtures per state. The car noise added speech database is enhanced using spectral subtraction before HMM parameter estimation for reducing model-mismatch caused by nonlinear distortion from spectral subtraction. The hardware module developed includes a microcontroller for host interface which processes the protocol between the DSP and a host.

• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
• /
• pp.62-72
• /
• 2001

The N-carbamoyl-D-amino acid amidohydrolase (D-carbamoylase) gene (dcb) from Agrobacterium tumefaciens AM 10 has been successfully cloned and expressed in Escherichia coli. Expression of D-carbamoylase gene under the 17 promoter in different host strains showed that the optimal expression was achieved in E. coli JM109 (DE3) with a 9-fold increase in enzyme production compared to the wild-type strain. The co-expression of the GroEL/ES protein with D-carbamoylase protein caused an in vivo solubilization of D-carbamoylase in an active form. The synergistic effect of GroEL/ES at 28$^{\circ}C$ led to 60 ％ solubilization of the total expressed target protein with a 6.2-fold increase in enzyme activity in comparison to that expressed without GroEL/ES and 43-fold increase in enzyme activity compared to A. tumefaciens AM 10. Attempts to express D-carbamoylase in an altered redox cytoplasmic milieu did not improve the enzyme production in an active form. The Histidyl-tagged D-carbamoylase was purified in a single step by Nickel-affinity chromatography and was found to have a specific activity of 9.5 U/mg protein.

• Molecules and Cells
• /
• 제39권9호
• /
• pp.705-713
• /
• 2016

The efficiency of Agrobacterium-mediated transformation in plants depends on the virulence of Agrobacterium strains, the plant tissue culture conditions, and the susceptibility of host plants. Understanding the molecular interactions between Agrobacterium and host plant cells is crucial when manipulating the susceptibility of recalcitrant crop plants and protecting orchard trees from crown gall disease. It was discovered that Arabidopsis voltage-dependent anion channel 1 (atvdac1) mutant has drastic effects on Agrobacterium-mediated tumorigenesis and growth developmental phenotypes, and that these effects are dependent on a Ws-0 genetic background. Genetic complementation of Arabidopsis vdac1 mutants and yeast porin1-deficient strain with members of the AtVDAC gene family revealed that AtVDAC1 is required for Agrobacterium-mediated transformation, and there is weak functional redundancy between AtVDAC1 and AtVDAC3, which is independent of porin activity. Furthermore, atvdac1 mutants were deficient in transient and stable transformation by Agrobacterium, suggesting that AtVDAC1 is involved in the early stages of Agrobacterium infection prior to transferred-DNA (T-DNA) integration. Transgenic plants overexpressing AtVDAC1 not only complemented the phenotypes of the atvdac1 mutant, but also showed high efficiency of transient T-DNA gene expression; however, the efficiency of stable transformation was not affected. Moreover, the effect of phytohormone treatment on competence to Agrobacterium was compromised in atvdac1 mutants. These data indicate that AtVDAC1 regulates the competence of Arabidopsis to Agrobacterium infection.

• Journal of Korea Trade
• /
• 제25권1호
• /
• pp.184-202
• /
• 2021

Purpose - This study empirically investigates the relationship between expatriate CEOs of multinational corporation (MNC) foreign subsidiaries and local philanthropy. Since corporate social responsibility (CSR) enables MNCs to achieve local legitimacy, this research argues that local philanthropy is a valuable strategic means for expatriate CEOs of foreign subsidiaries to secure local legitimacy. Design/methodology - To investigate our argument, we use a sample of 5,459 observations from 576 foreign subsidiaries of MNCs in Korea between 2002 and 2016. We conduct a random-effects panel Tobit regression with subsidiary CEO having foreign nationality as the independent variable and local philanthropy as the dependent variable. Findings - Our main findings are that expatriate CEOs of foreign subsidiaries are more actively engaged in local philanthropy. In addition, the positive relationship between expatriate CEOs and local philanthropy is weaker as their tenure increases. Originality/value - How expatriate CEOs overcome their weak local legitimacy as foreigners in a host country has remained unclear because existing studies mainly focused on the control and coordination aspects of staffing expatriates in CEO positions of foreign subsidiaries. This study broadens the literature on subsidiary CEO staffing and CSR activities of MNCs by identifying complementary relationships between expatriate CEOs and corporate philanthropy in the host country.

• IMMUNE NETWORK
• /
• 제24권1호
• /
• pp.11.1-11.18
• /
• 2024

IL-15 belongs to the common gamma chain cytokine family and has pleiotropic immunological functions. IL-15 is a homeostatic cytokine essential for the development and maintenance of NK cells and memory CD8⁺ T cells. In addition, IL-15 plays a critical role in the activation, effector functions, tissue residency, and senescence of CD8⁺ T cells. IL-15 also activates virtual memory T cells, mucosal-associated invariant T cells and γδ T cells. Recently, IL-15 has been highlighted as a major trigger of TCR-independent activation of T cells. This mechanism is involved in T cell-mediated immunopathogenesis in diverse diseases, including viral infections and chronic inflammatory diseases. Deeper understanding of IL-15-mediated T-cell responses and their underlying mechanisms could optimize therapeutic strategies to ameliorate host injury by T cell-mediated immunopathogenesis. This review highlights recent advancements in comprehending the role of IL-15 in relation to T cell responses and immunopathogenesis under various host conditions.

• Molecules and Cells
• /
• 제46권11호
• /
• pp.710-724
• /
• 2023

The plant defense responses to microbial infection are tightly regulated and integrated with the developmental program for optimal resources allocation. Notably, the defense-associated hormone salicylic acid (SA) acts as a promoter of flowering while several plant pathogens actively target the flowering signaling pathway to promote their virulence or dissemination. Ralstonia pseudosolanacearum inject tens of effectors in the host cells that collectively promote bacterial proliferation in plant tissues. Here, we characterized the function of the broadly conserved R. pseudosolanacearum effector RipL, through heterologous expression in Arabidopsis thaliana. RipL-expressing transgenic lines presented a delayed flowering, which correlated with a low expression of flowering regulator genes. Delayed flowering was also observed in Nicotiana benthamiana plants transiently expressing RipL. In parallel, RipL promoted plant susceptibility to virulent strains of Pseudomonas syringae in the effector-expressing lines or when delivered by the type III secretion system. Unexpectedly, SA accumulation and SA-dependent immune signaling were not significantly affected by RipL expression. Rather, the RNA-seq analysis of infected RipL-expressing lines revealed that the overall amplitude of the transcriptional response was dampened, suggesting that RipL could promote plant susceptibility in an SA-independent manner. Further elucidation of the molecular mechanisms underpinning RipL effect on flowering and immunity may reveal novel effector functions in host cells.

• 한국통신학회논문지
• /
• 제35권7A호
• /
• pp.688-696
• /
• 2010

PMIPv6(Proxy MIPv6)는 네트워크 기반의 도메인 내 이동(network localized mobility)만을 지원하는 프로토콜이며 글로벌 이동은 지원하지 않는다. 한 도메인(LMD)에서 MN(Mobile Node)는 통신중에 IP 계층의 이동에 필요한 시그널링에 간여하지 않으며, 오직 PMIPv6 홈 주소(home address)만을 사용한다. 따라서 MN가 다른 도메인으로 이동을 하면 MN은 PMIPv6의 홈 주소를 변경해야 한다. 이 때 호스트 기반의 시그널링(host-based signaling)이 활성화되고, 따라서 PMIPv6의 네트워크 기반 이동성 관리가 유지될 수 없다. 또한 MN가 글로벌 이동을 지원하지 않는 경우에는 CN(Correspondent Node)와 통신 세션을 유지할 수 없다. 본 논문에서는 PMIPv6에서 글로벌 이동(Global Mobility)을 지원하는 방안을 제안하였으며, 이동 프로토콜 스택(mobility protocol stack)을 갖지 않는 MN가 다른 도메인으로 이동을 해도 통신 세션을 유지할 수 있다. 따라서 본 논문에서 제안한 방법은 글로벌 이동을 지원하면서 동시에 PMIPv6의 장점을 유지할 수 있다. 또한 제안된 방법과 PMIPv6의 성능을 비교함으로써 제안된 방법의 성능이 향상되었음을 알 수 있었다.