• Title/Summary/Keyword: host factors

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Examining the factors influencing leaf disease intensity of Kalopanax septemlobus (Thunb. ex Murray) Koidzumi (Araliaceae) over multiple spatial scales: from the individual, forest stand, to the regions in the Japanese Archipelago

  • Sakaguchi, Shota;Yamasaki, Michimasa;Tanaka, Chihiro;Isagi, Yuji
    • Journal of Ecology and Environment
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    • v.35 no.4
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    • pp.359-365
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    • 2012
  • We investigated leaf disease intensity of Kalopanax septemlobus (prickly castor oil tree) caused by the parasitic fungus Mycosphaerella acanthopanacis, in thirty natural host populations in the Japanese Archipelago. The disease intensity observed for individual trees were analyzed using a generalized additive model as a function of tree size, tree density, climatic terms and spatial trend surface. Individual tree size and conspecific tree density were shown to have significant negative and positive effects on disease intensity, respectively. The findings suggest that the probability of disease infection is partly determined by dispersal of infection agents (ascospores) from the fallen leaves on the ground, which can be enhanced by aggregation of host trees in a forest stand. Regional-scale spatial bias was also present in disease intensity; the populations in northern Japan and southern Kyushu were more severely infected by the fungus than those in southwestern Honshu and Shikoku. Regional variation of disease intensity was explained by both climatic factors and a trend surface term, with a latitudinal cline detected, which increases towards the north. Further research should be conducted in order to understand all of the factors generating the latitudinal cline detected in this study.

Factors Affecting Appressorium Formation in the Rice Blast Fungus Magnaporthe grisea (벼 도열병균의 부차기 형성에 미치는 요인 분석)

  • 이승철;강신호;이용환
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.413-417
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    • 1998
  • Magnaporthe grisea, the casual agent of rice blast, requires formation of an appressorium, a dome-shaped and well melanized infection structure, to penetrate its host. Environmental cues that induce appressorium formation include hydrophobicity and hardness of contact surface and chemicals from its host. Artificial surfaces are widely used to induce appressorium formation, but frequencies of appressorium induction are not always consistent. To understand variable induction of appressorium formation in M. grisea, several factors were tested on GelBond. High levels of appressorium formation were induced over a wide range of temperature (20~3$0^{\circ}C$) and pH (4~7). spore age up to 3-week-old did not significantly affect appressorium formation, but only a few apressoria on GelBond. However, adenosine specifically inhibited appressorium formation. Adenosine inhibition of appressorium formation was restored by exogenous addition of cAMP. Germ tube tips of M. grisea maintained the ability to differentiate appressoria by chemical inducers on GelBond at least up to 16 h after conidia germination. These results suggest that environmental factors have little effect on the variable induction of appressorium formation on the artificial surface in M. grisea.

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Influences of Firm Characteristics and the Host Country Environment on the Degree of Foreign Market Involvement (기업특성과 호스트국가 환경이 해외시장 관여도에 미치는 영향에 관한 연구)

  • Maktoba, Omar;Nwankwo, Sonny
    • Journal of Global Scholars of Marketing Science
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    • v.19 no.2
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    • pp.5-16
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    • 2009
  • Against the backdrop of the increasing trend towards economic globalisation, many international firms are indicating that decisions on how to enter foreign markets remains one of the key strategic challenges confronting them. Despite the rich body of literature on the topic, the fact that these challenges have continued to dominate global marketing strategy discourses point to someevident lacunae. Accordingly, this paper considers the variables, categorised in terms of firm contexts (standardisation, market research, competition, structure, competitive advantage) and host country-contexts (economic development, cultural differences, regulation and political risk), which influence the degree of involvement of UK companies in overseas markets. Following hypotheses were drawn from literature review: H1: The greater the level of competition, the higher the degree of involvement in the overseas market. H2: The more centralised the firm's organisation structure, the higher the degree of involvement in the overseas market. H3a: The adoption of a low cost-approach to competitive advantage will lead to a higher degree of involvement. H3b: The adoption of an innovation-approach to competitive advantage will lead to a higher degree of involvement. H3c: The adoption of a market research approach to competitive advantages will lead to a higher degree of involvement. H3d: The adoption of a breadth of strategic target-approach to competitive advantage will lead to a lower degree of involvement. H4: The higher the degree of standardisation of the international marketing mix the higher the degree of involvement. H5: The greater the degree of economic development in the host market, the higher the degree of involvement. H6: The greater the cultural differences between home and host countries, the lower the degree of involvement. H7: The greater the difference in regulations between the home country and the host country, the lower the degree of involvement. H8: The higher the political risk in the host country, the lower the degree of involvement. A questionnaire instrument was constructed using, wherever possible, validated measures of the concepts to serve the aims of this study. Following two sets of mailings, 112 usable completed questionnaires were returned. Correlation analysis and multiple regression analysis were used to analyze data. Statistically, the paper suggests that factors relating to the level of competition, competitive advantages and economic development are strong in influencing foreign market involvements. On the other hand, unexpectedly, cultural factors (especially individualism/collectivism and low and high power distance dimensions) proved to have weak moderating effects. The reason for this, in part, is due to the pervading forces of globalisation and the attendant effect on global marketing. This paper has contributed to the general literature in a way that point to two mainimplications. First, with respect to research on national systems, the study may hold out some important lessons especially for developing nations. Most of these nations are known to be actively seeking to understand what it takes to attract foreign direct investment, expand domestic market and move their economies from the margin to the mainstream global economy. Second, it should be realised that competitive conditions remain in constant flux (even in mature industries and mature economies). This implies that a range of home country factors may be as important as host country factors in explaining firms' strategic moves and the degree of foreign market involvement. Further research can consider the impact of the home country environment on foreign market involvement decisions. Such an investigation will potentially provide further perspectives not only on the influence of national origin but also how home country effects are confounded with industry effects.

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Effects of N. P. K. and sugar contents of different host-plants on the Biology of the fall web-worm, Hyphantria cunea DRURY (기주식물의 N.P.K. 및 Sugar의 함량이 흰불나방에 미치는 생물학적 영향)

  • Chung K. H.;Choi S. Y.
    • Korean journal of applied entomology
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    • v.5_6
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    • pp.39-46
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    • 1968
  • This experiment was conducted to study the effects of nitrogen, phosphorus, potassium, and sugar contents in the loaves of five different host plants on tile larval period, pupal period, pupal period, number of eggs in the ovary, and the tolerance of fall webworm larvae (Hyphantria cunea DRURY) to gamma-BHC. The results obtained in this study were as follows; 1. The contents of nitrogen, phosphorus, potassium, and sugar in tile leaves tested were varied with the species of the host plants and sampling time. 2. The larval and pupal periods were also varied with the host plants and generation of the fall webworm. The shortest larval and pupal periods were found in the mulberry and the longest ones in the apple tree, and their periods were shortened much more in the second generation. Except the relation between the larval period and tile nitrogen content in the first generation, significant correlation could not be found between their periods and nitrogen, phosphorus, potassium and surgar contents. The nitrogen contents among the other chemical factors, however, might be much more influenced on their developing period. 3. The pupal weight and number of eggs in the ovary were also varied with the host plants. sex, and generation. In both generations, the greatest pupal weight and number of eggs per female were found in the mulberry and the least ones in the apple tree. No significant correlation could be found between the pupal weight and number of eggs per female and the chemical factors analyzed. However, only the nitrogen content seemed to be highly related to the pupal weight and number of eggs. 4. The tolerance of the fall webworm larval to gamma-BHC was highly varied with the host plants. The host order o( the larval tolerance level to gamma-BHC was box-elder, popla, platanus, apple tree, and mulberry. There was no significant correlation between the larval tolerance to the BHC and the chemical factors analyzed. However, the larvae fed on the host plant with higher pottassium content were shown a tendency to be higher tolerant.

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Analysis of TCP/IP Protocol for Implementing a High-Performance Hybrid TCP/IP Offload Engine (고성능 Hybrid TCP/IP Offload Engine 구현을 위한 TCP/IP 프로토콜 분석)

  • Jang Hankook;Oh Soo-Cheol;Chung Sang-Hwa;Kim Dong Kyue
    • Journal of KIISE:Computer Systems and Theory
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    • v.32 no.6
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    • pp.296-305
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    • 2005
  • TCP/IP, the most popular communication protocol, is processed on a host CPU in traditional computer systems and this imposes enormous loads on the host CPU. Recently TCP/IP Offload Engine (TOE) technology, which processes TCP/IP on a network adapter instead of the host CPU, becomes an important way to solve the problem. In this paper we analysed the structure of a TCP/IP protocol stack in the Linux operating system and important factors, which cause a lot of loads on the host CPU, by measuring the time spent on processing each function in the protocol stack. Based on these analyses, we propose a Hybrid TOE architecture, in which functions imposing much loads on the host CPU are implemented using hardware and other functions are implemented using software.

Influence of abiotic factors on seasonal incidence of pests of tasar Silkworm Antheraea mylitta D.

  • Siddaiah, Aruna A.;Prasad, Rajendra;Rai, Suresh;Dubey, Omprakash;Satpaty, Subrat;Sinha, Ravibhushan;Prsad, Suraj;Sahay, Alok
    • International Journal of Industrial Entomology and Biomaterials
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    • v.29 no.1
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    • pp.135-144
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    • 2014
  • Rearing of tropical tasar silkworm, Antheraea mylitta Drury is mainly conducted in outdoor on Terminalia tomentosa W. & A. a nature grown primary host plant available in forest and also on raised primary host plant Terminalia arjuna Bedd. Temperature, relative humidity and rainfall are the main environmental factors for occurrence of pests (parasites and predators) of tasar silkworm during I, II and III crop rearing in the tropical tasar producing zones. The present study was aimed to study the influence of abiotic factors on prevalence of tasar silkworm pests. The study was conducted at different agro-climatic regions viz., Central Tasar Research &Training Institute, Ranchi, Jharkhand, Regional Extension Centre, Katghora, Chattisgarh and Regional Extension Centre, Hatgamaria during 2010-13 covering 3 seed crop and 6 commercial crops. Data on incidence of tropical tasar silkworm endo-parasitoids like Uzi Fly, Blepharipa zebina Walker and Ichneumon fly (Yellow Fly), Xathopimpla pedator, Fabricius and Predators such as Stink bug (Eocanthecona furcellata Wolf), Reduviid bug (Sycanus collaris Fabricius) and Wasp (Vespa orientalis Linnaeus) was recorded Weekly. The meteorological data was collected daily. Data was collected from 4 different agro-climatic zones of tasar growing areas. Analysis of the data revealed a significant negative correlation between abiotic factors and incidence of ichneumon fly and uzi fly. Based on the 3 years data on prevalence of pests region-wise pest calendars and prediction models were developed.

Chicken FMRP Translational Regulator 1 (FMR1) Promotes Early Avian Influenza Virus Transcription without Affecting Viral Progeny Production in DF1 Cells

  • Woo, Seung Je;Park, Young Hyun;Han, Jae Yong
    • Korean Journal of Poultry Science
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    • v.48 no.2
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    • pp.81-90
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    • 2021
  • Avian influenza viruses (AIVs) must utilize host cellular factors to complete their life cycle, and fragile X mental retardation protein (FMRP) has been reported to be a host factor promoting AIV ribonucleoprotein (vRNP) assembly and exports vRNP from the nucleus to the cytoplasm. The functional role of chicken FMRP translational regulator 1 (cFMR1) as a host factor of AIV is, however, poorly understood. In this study, we targeted the cFMR1 gene in DF1 cells using clustered regularly interspaced short palindromic repeats/Cas9-mediated genome editing to examine the functional role of cFMR1 as a host factor of AIV. We found that cFMR1 stimulated viral gene transcription during early stages of the viruses' life cycle and did not affect viral progeny production and viral polymerase activity in DF1 cells 24 hours post infection. cFMR1 overexpression did not exert significant effects on virus production, compared to the control. Therefore, unlike in mammalian systems (e.g., humans or mice), cFMR1 did not play a pivotal role in AIV but only seemed to stimulate viral proliferation during early stages of the viral life cycle. These results imply that the interplay between host factors and AIV differs between mammals and avian species, and such differences should be considered when developing anti-viral drugs for birds or establishing AIV-resistant bird models.

Adaptation of the Hypoosmotic Swelling Test to Evaluate Membrane Integrity of Boar Spermatozoa

  • Jang, Hyun-Yong;Cheong, Hee-Tae;Hwang, Hwan-Sub;Kim, Jong-Taek;Park, Choon-Keun;Lee, Hak-Kyu;Yang, Boo-Keun
    • Reproductive and Developmental Biology
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    • v.31 no.2
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    • pp.121-126
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    • 2007
  • The objective of this study was to establish the optimal conditions for hypoosmotic swelling (HOS) test to assess the functional integrity of the membranes of boar fresh or frozen/thawed spermatozoa. When pooled semen sample was incubated for 30 min at $37^{\circ}C$ with different test solution of varied osmolarity, the highest percentage of HOS positive spermatozoa was observed in a 150 mOsmol fructose/Na-citrate solution (33.6%). Incubation time did not affect significantly the score of HOS positive spermatozoa observed in a 150 mOsmol fructose/Na-citrate solution at $37^{\circ}C$, but the osmolarity affected the score of HOS positive spermatozoa under the same condition above. Fresh semen was significantly better than frozen/thawed semen in semen parameters evaluated such as motility, viability, membrane integrity and lipid peroxidation (p<005). In the relationships of sperm parameters, motility vs viability, motility vs membrane integrity and viability vs membrane integrity were positively correlated ($0.82{\sim}0.94$) but lipid peroxidation vs other estimated factors was negatively correlated ($- 0.90{\sim}- 0.98$). Among the evaluation methods, motility vs Viability, motility vs membrane integrity and lipid peroxidation vs other estimated factors were significantly correlated (p<0.05). These results of this. study indicate that the optimal condition of HOST in boar spermatozoa is a 150 mOsmol fructose/Na-citrate solution for 30 min incubation at $37^{\circ}C$ and HOST can substitute the examination of motility, viability and lipid peroxidation.

Structural and Mechanistic Insights into the Tropism of Epstein-Barr Virus

  • Mohl, Britta S.;Chen, Jia;Sathiyamoorthy, Karthik;Jardetzky, Theodore S.;Longnecker, Richard
    • Molecules and Cells
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    • v.39 no.4
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    • pp.286-291
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    • 2016
  • Epstein-Barr virus (EBV) is the prototypical ${\gamma}$-herpesvirus and an obligate human pathogen that infects mainly epithelial cells and B cells, which can result in malignancies. EBV infects these target cells by fusing with the viral and cellular lipid bilayer membranes using multiple viral factors and host receptor(s) thus exhibiting a unique complexity in its entry machinery. To enter epithelial cells, EBV requires minimally the conserved core fusion machinery comprised of the glycoproteins gH/gL acting as the receptor-binding complex and gB as the fusogen. EBV can enter B cells using gp42, which binds tightly to gH/gL and interacts with host HLA class II, activating fusion. Previously, we published the individual crystal structures of EBV entry factors, such as gH/gL and gp42, the EBV/host receptor complex, gp42/HLA-DR1, and the fusion protein EBV gB in a postfusion conformation, which allowed us to identify structural determinants and regions critical for receptor-binding and membrane fusion. Recently, we reported different low resolution models of the EBV B cell entry triggering complex (gHgL/gp42/HLA class II) in "open" and "closed" states based on negative-stain single particle electron microscopy, which provide further mechanistic insights. This review summarizes the current knowledge of these key players in EBV entry and how their structures impact receptor-binding and the triggering of gB-mediated fusion.

Analysis of the morphological change and the expression of secretory leukocyte protease inhibitor (SLPI) in various cell lines after lipopolysaccharide stimulation

  • Choi, Baik-Dong;Choi, Jeong-Yoon;Jeong, Soon-Jeong;Park, Joo-Cheol;Kim, Heung-Joong;Bae, Chun-Sik;Lim, Do-Seon;Jeong, Moon-Jin
    • 한국전자현미경학회:학술대회논문집
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    • 2005.11a
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    • pp.127-129
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    • 2005
  • Bacterial lipopolysaccharide(LPS) is can stimulate the most LPS-responsive cells in the mammalian host. The macrophage response to LPS can protect the host from infection but high levels, contribute to systemic inflammatory response syndrome and destruction of host itself, The previously study, secretory leukocyte pretense inhibitor (SLPI) was known LPS-induced product of macrophage and had the function that antagonizes their LPS-induced activation of pro-inflammation signaling factors. Purpose of this study was to identify the expression of SLPI involving the infection in various cell lines including odontoblast cell line. Therefore, we conducted in vitro researches, which treated the LPS to the MDPC-23, and compared to NIH3T3, RAW264.7. To investigate the expressionof SLPI in mRNA level, the methods was used RT-PCR and western blotting for protein expression of SLPI. Moreover, we performed the scanning electron microscopic (SEM) observation for the morphological change. This work was supported by Korea Science and Engineering Foundation.

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