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Bacterial Contamination Reduction of Minimally Processed Agricultural Products using Antibacterial Foods and Molecular Biological Analysis (항균성 식품을 이용한 간편섭취 농산물 미생물오염의 감소 및 분자생물학적 분석)

  • Kim, Jin-Ah;Lee, Sung-Deuk;Hwang, Kwang-Ho;Song, Mi-Ok;Park, Jung-Eun;Kim, Da-Mi;Chung, Ae-Hee;Oh, Young-Hee
    • Journal of Food Hygiene and Safety
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    • v.31 no.1
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    • pp.8-14
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    • 2016
  • The purposes of this study are to confirm ways to reduce bacteria of minimally processed agricultural products, using antibacterial foods that are easily available in home and to improve bacterial hygienic condition of them. We chose garlic-allicin, ginger-gingerol, green tea-catechin, cinnamon-cinnamic aldehyde, wasabi-allyl isothiocyanate as antibacterial foods and their unique antibiotic materials. We confirmed the better washing effect when these antibiotic extracts were used, compared to washing effect by only distilled water. Their antibiosis was proved by statistical processing. PFGE (Pulsed Field Gel Electrophoresis) of Bacillus cereus shows continuous contamination probability of minimally processed vegetables by same product suppliers and the necessity of systematic measures against bacterial contamination.

A Bibliographical Study of Korean Fan Fried Side dishes(Jeon) in Korean Literatures before the 1900s (1900년대 이전 문헌에 기록된 전 조리법의 문헌적 고찰)

  • Choi, Young-Jin
    • Korean journal of food and cookery science
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    • v.28 no.5
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    • pp.629-639
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    • 2012
  • Jeon refers to a type of Korean cuisine used as a side dish, made with various ingredients such as meat, vegetables, or fish, mixed with flour batter and coated with egg batter and then pan-fried on both sides with oil so that heat spreads through them well. The aim of this study was to provide a cornerstone of further research on Korean dietary life, by reviewing types, recipes and names of Jeon recorded in ancient cookbooks (Korean literatures) and by analyzing changes in recipes to make Jeon, which our ancestors used wisely as one type of side dish. Considering developments and changes recorded in old documents, the Jeon recipe appeared relatively later than the recipes for grilling, steaming et al, which had been developed much earlier, and it had not been recorded until the 1600s. Changes of Jeon recipes by time period are as follows. In the 1600s, there were three recipes: frying only with grain batter after preparation of ingredients, frying right after preparation of ingredients, and putting ingredients on already fried watery batter. These three recipes were still used in the 1700s, in addition to a new recipe, in which ingredients were oil-fried to be skewered. Today's recipe in which prepared ingredients are fried after getting coated with flour and egg only appeared in 1800s. This has been the main recipe for Jeon ever since. In that time period, there was more variety of recipes and ingredients than before. For instance, Jeon was used for soup or steamed dishes instead of being served as a dish itself. Buchimgae with mixed ingredients was also considered Jeon. In the 1900s, there appeared more names for Jeon as more sorts of ingredients got used, even though there were no new recipes for Jeon. The above-mentioned historical records show that traditional recipes for Jeon have been applied to various dishes, using diverse ingredients, and it might be a smart solution to today's problematic dietary habits involving excessive intake of nutrients, in that it provides a healthy way to add fat. In the same sense, the recipe for Jeon can play an active role in internationalization of Korean foods, in which healthiness is a main feature. According to ancient documents, the recipes for Jeon were used even for meals that were not side dishes, such as Jun-Gwa (Jung-Gwa), Jun-Yak and fried rice-cake. Also, there were dishes using the same recipes even without carrying the name of Jeon, like Buchim or Jijim. This might be worthy of further examination in culinary science.

Changes in Glucosinolate Component Content in Urine After Ingestion of Fresh and Cooked Broccoli (신선한 브로콜리와 조리된 브로콜리 섭취 후 소변으로 배설되는 Glucosinolates 대사물질의 함량 변화)

  • Hwang, Eun-Sun
    • Korean journal of food and cookery science
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    • v.26 no.6
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    • pp.804-810
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    • 2010
  • Sulforphane (SF) is a family of biologically active compound that is distributed widely in broccoli. Although studies in rodents have shown that these compounds are effective and versatile inhibitors of tumorigenesis, the role of dietary SF in protection against human cancers remains to be established. The objective of this study was to explore the quantitative relationship between the dietary intake of cruciferous vegetables and urinary excretion of SF. The effects of dietary broccoli on the body's ability to detoxify were studied in six male subjects between the ages of 22~30 years. Study included administering a glucosinolate-free diet for 8 days (control period). The broccoli diet was further subdivided into two periods; 250 g broccoli was fed per day during the first three days and 500 g broccoli was fed per day during the latter three days. After an 8-day washout period, a second experiment was conducted. The same protocol was used with the exception that uncooked broccoli was consumed. Urinary SF mercapturate was measured to determine the bioavailability of broccoli. The linear trend for mercapturate excretion was dose-dependent, resulting in 3.8- and 1.9-fold increase by the third and six days, respectively, compared to the control. Lower amount of SF-NAC conjugate was detected in cooked broccoli compared to fresh broccoli suggesting cooking may have caused a significant loss in glucosinolates in cruciferous vegetables. Therefore, SF can be used as a biomarker for intake of cruciferous vegetables.

Batch Scale Storage of Sprouting Foods by Irradiation Combined with Natural Low Temperature -V. Cooking Qualities of Irradiated Chestnut after Long-term Storage- (방사선조사(放射線照射)와 자연저온(自然低溫)에 의한 발아식품(發芽食品)의 Batch Scale저장(貯藏)에 관(關)한 연구(硏究) -제5보(第五報) : 장기저장(長期貯藏)된 조사(照射)밤의 조리적성(調理適性)에 대하여-)

  • Yang, Ho-Sook;Kim, Jong-Gun;Cho, Han-Ok;Byun, Myung-Woo;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.15 no.3
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    • pp.238-244
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    • 1983
  • The cooking quality of irradiated chestnut after longterm storage were evaluated. 1. Color degree and tannin content of irradiated chestnut were slightly increased with the storage, but there was a little difference according to the radiation dose after nine months storage. 2. The main component of free sugars in the irradiated chestnut were identified as sucrose, glucose, fructose and the amino acids of chestnut were identified in the decreasing order of glutamic acid, aspartic acid, leucine, arginine, glycine, alanine, serine, pheylalanine, threonine, valine, isoleucine, tyrosine, methionine and cystein. Free sugars and amino acids of 25 Krad irradiated group showed a little difference compared with those of control group after nine months storage. 3. The calorie of candied chestnut prepared from nine months stored was marked 199 Kcal/100g of edible parts compared with 159 Kcal of raw chestnut. 4. Texture and sensory evaluation of candied chestnut prepared from nine months stored were better in 20-25 Krad irradiated group than in control group.

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Batch Scale Storage of Sprouting Foods by Irradiation Combined with Natural Low Temperature -Ⅳ. Storage of Chestnuts- (방사선조사(放射線照射)와 자연저온(自然低溫)에 의한 발아식품(發芽食品)의 Batch Scale저장(貯藏)에 관(關)한 연구(硏究) -제4보(第四報) : 밤의 저장(貯藏)-)

  • Cho, Han-Ok;Yang, Ho-Sook;Byun, Myung-Woo;Kwon, Joong-Ho;Kim, Jong-Gun
    • Korean Journal of Food Science and Technology
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    • v.15 no.3
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    • pp.231-237
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    • 1983
  • In order to develop the commercial storage method of chestnut by irradiation combined with natural low temperature, a chestnut variety Ok-gwang was stored in a natural low temperature storage room ($450{\times}650{\times}250$ cmH; year-round temperature change, $2-17^{\circ}C$; R.H., 80-85%) on batch scale followed by irradiation with optimum dose level. Sprouting rate of chestnut was 100% after seven month storage in control whereas that of 20-25 Krad irradiated group was only 5-15%. In comparison of rotting rate, weight loss and texture, 25 Krad irradiated group was better than that of control. Moisture and reducing sugar were increased in contrast with the decrease of total sugar during nine month storage and these changes were more remarkable in control. Ascorbic acid content was slightly decreased both in control and irradiated group; more decrement was noticed in control.

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Department of DNA Chromatographic System for On-Site Detection of Food-Contaminating Bacteria (식중독균 현장탐지를 위한 DNA 크로마토그래피 분석시스템의 개발)

  • 김석하;정우성;백세환
    • KSBB Journal
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    • v.18 no.3
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    • pp.190-196
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    • 2003
  • An analytical system detecting DNA particularly utilizing a concept of membrane strip chromatography initially applied to home-version tests for, such as, pregnancy and ovulation has been developed. We have chosen S. typhimurium as model analyte among food-contaminating microorganisms that occurred in high frequencies, and invA gene, as a detection target, specific to Salmonella species. This gene was able to be amplified by PCR under optimal conditions employing newly designed primers in our laboratory. The PCR product was specifically measured via hybridization between the analyte and a DNA probe, which was a totally different feature from the conventional gel electrophoresis detecting the products based only on the molecular size. It is notable thar the DNA probe sequence was specially designed such that no separation of excess primers present after PCR was required. This was immobilized on a nitrocellulose (NC) membrane via streptavidin-biotin linkage minimizing a steric effect when the hybridization with the amplified DNA took place. The analyrical system detected the microorganism in a concentration of minimum $10^3$ cfu/mL (i.e., 10 cells per system), estimated from the standard curve, 20 to 40 minutes after adding the sample. This sneitivity was approximately 10 times higher than that of gel electrophoresis as an analytical tool conventionally used. Furthermore, the assay was able to be run at room temperature, which would ofter an extra advantage to users.

The study for the mouse cerebellum developments irradiated with γ-ray during embryogenesis (감마선 조사에 의한 발생과정 중인 마우스의 소뇌발달에 관한 연구)

  • Park, Il-Kwon;Lee, Kyoung-Youl;Park, O-Sung;Kim, Sung-Hwa;Lee, Guen-Jwa;Lee, Kang-Yi;Min, Tae-Sun;Yook, Hong-Sun;Byun, Myung-Woo;Kim, Moo-Kang
    • Korean Journal of Veterinary Research
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    • v.45 no.2
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    • pp.139-149
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    • 2005
  • In order to study about the lobule and layer formation and cell migration of the mouse cerebellum from at the birth to 15th day effected by 2.5, 5 and 10 Gy r-raddiation at the 19th pregnancy. The routine tissue preparation and staining procedure, Immunohistochemical staining method by the several antibody and western brotting method were utilized from the birth to the15th day. The results were as followings. 1. The body and cerebellum weights were more slowly increase of the the 2.5 Gy, 5 Gy and 10 Gy irradiation group compare to the control group, and the health condition of the 2.5 Gy group was a little bad. but the 10 Gy group was more severe and begun to die from the 12th day after birth. 2. The thickness, proliferation and migration of the 2.5, 5 and 10 Gy irradiated external granular cells from the maginal zone to the medullary area forming the molecular layer from the 6th day to the 15th day after birth were thinner, weaker and more slower according to the radiated dosages than the control group in the cresyl violet staining. 3. The proliteration, migration and lobulation of the 5 Gy radiated groups from the first day to the 15th day after birth were more weak, incomplete and irregular shape in the immunostaining with Dab, Cdk5, P35, calbindin and Zebrin antibody. 4. In the western blotting analysis using the Reelin, Dab, Cdk5 and P35 antibody. The Bands were in the 60 KD, 80 KD, 33 KD and 35 KD, and there were no differences between the control and irradiated groups in the molecular band except the Reelin. 5. As a results, the proliferation and migration of the outer granular and purkinje cells, and lobulation of the cerebellum by the several dosaege of the ${\gamma}$-ray radiation were proportionally incomplete according to dosage.

A Comparison of the Preference and Consumption Status of Non-Alcohol and Alcohol Beverages of Korean and Chinese University Students in the Jeonbuk Area (전북지역 한국대학생과 중국대학생의 일반음료와 알코올음료의 기호도와 섭취실태 비교)

  • Rho, Jeongok;Chang, Eunha
    • Korean Journal of Human Ecology
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    • v.21 no.4
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    • pp.805-817
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    • 2012
  • This study was conducted to investigate the preference and consumption status of non-alcohol and alcohol beverages of Korean and Chinese university students in the Jeonbuk area. Self-administered questionnaires were collected from 241 Korean and 198 Chinese students. Statistical data analysis was completed using SPSS v. 12.0. 'Water' was the most commonly consumed non-alcohol beverages by all Korean and Chinese students. The intake frequency of 'carbonated drink' (p<.001), 'fruits/vegetable juice' (p<.05), and 'vinegar drink' (p<.01) of Chinese male students was significantly higher than the Korean male students. The intake frequency of 'ionic drink' (p<.001; p<.01) of Korean male and female students was significantly higher than the Chinese male and female students. The intake frequency of 'fruits/vegetable juice' (p<.001), 'milk' (p<.01), and 'soymilk' (p<.05) of Chinese female students was higher than the Korean female students. For the choosing the non-alcohol beverages, the Chinese female students were more health-oriented than the Korean female students (p<.01). 'Beer' was the most commonly consumed alcohol beverages by the Korean male and female and Chinese male students whereas 'Wine' was the most commonly consumed alcohol beverages by the Chinese female students. The intake frequency of 'Soju' of Korean male (p<.001) and female (p<.001) students was higher than the Chinese male and female students. The intake frequency of 'Wine' of Chinese male (p<.05) and female (p<.001) students higher than the Korean male and female students. Compared with the Chinese female students, the Korean female students more funds have to spend, in order to buy alcohol beverages (p<.01). More 81% of the Korean male and 80% of the female students reported drinking alcohol in 'beer house' whereas 78% of the Chinese male and 65% of female students drunken the alcohol in the 'beer house' or at 'home' (p<.01; p<.001). In conclusion, a practically and foreigner-friendly alcohol policies of university should be devised to reduce the alcohol consumption of students and related problems.

Comparison of Microwave and Conventional Cooking Methods on the Nutritional Composition of Potatoes -(1) Changes of Proximate Composition, Minerals and Water-soluble Vitamins- (감자의 몇가지 영양성분(營養成分)에 대한 마이크로파(波)와 재래적(在來的) 조리방법(調理方法)의 비교(比較) -1. 일반성분(一般成分), 무기질(無機質) 및 수용성(水溶性) 비타민의 변화에 대하여-)

  • Yoo, Yang-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.2
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    • pp.171-176
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    • 1985
  • The effects of cooking methods by boiling, electric oven and microwave on the proximate composition, minerals and water-soluble vitamins of two major Korean potato varieties (Soomi and Namjac) were studied. The dry matter content was increased after the cooking by the methods of electric oven and microwave, however, the crude fiber content was highly decreased by both methods. The crude ash content was not affected by the cooking methods used. The potassium content was increased by electric oven and microwave methods, and iron content was increased by electric oven baking, however, the copper content was significantly decreased by the electric oven and microwave cooking methods. The macrominerals (phosphorus, magnesium, calcium) and microminerals (zinc, manganese, nickel, lead, cadmium) content present initially in potatoes have not been changed by the cooking methods employed in this study. Relative retention value of ascorbic acid was the lowest and the value of thiamin was the highest in the potatoes cooked by microwave. The retention values of riboflavin and folic acid obtained. after boiling were similar to those after microwave cooking, and both of these treatments were superior to the electric oven baking. The retention values of niacin and vitamin $B_6$ varied between 93 and 100% depending on the cooking methods used. Overall, with a few exceptions, the retention values of water-soluble vitamins in boiled and microwave cooked potatoes were superior to those of the electric oven baked.

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Minerals, Total Nitrogen and Free Amino Acid Contents in Shank Bone Stock according to Boiling Time (사골 용출액 중의 무기질, 총질소, 아미노산의 함량 변화)

  • Park, Dong-Yean
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.3
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    • pp.243-248
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    • 1986
  • This study was examined the contents of calcium, phosphorus, magnesium, sodium, potassium, total nitrogen (total N) and free amino acid in shank bone stock as results of boiling for eight, twelve, sixteen and twenty hours. The results were as follows. 1) Contents of calium, sodium and potassium were increased according to boiling time, Content of calcium was more than two times after boiling for twenty hours as compared with eight hours. The contents of potassium and sodium were increased slightly but that of magnesium was constant in the course of boiling. The content of phosphorus was showed maximum value after toiling for eight hours and was decreased after boiling for twelve hours. 2) The content of total N was increased according to boiling time and showed moximum value after boiling for twenty hours. Total N was extracted more than two times after boiling for twenty hours as compared with eight hours. The content of free amino acid was little at various boiling time. The contents of glycine, glutamic acid and serine were increased according to boiling time. Glycine was the most abundant free amino acid and was followed in order of glutamic acid. alanine and serine.

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