• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1696-1700
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• 2016

In order to discover plant-derived signaling pathway inhibitors with antifungal properties, a two-component screening system utilizing the calcineurin and Hog1 mitogen-activated protein kinase pathways responsible for the virulence networks of Cryptococcus neoformans was employed, owing to the counter-regulatory actions of these pathways. Of the 1,000 plant extracts tested, two bioactive compounds from Miliusa sinensis were found to act specifically on the calcineurin pathway of C. neoformans. These compounds, identified as pashanone and 5-hydroxy-6,7-dimethoxyflavanone, exhibited potent antifungal activities against various human pathogenic fungi with minimum inhibitory concentration values ranging from 4.0 to >128 μg/ml.

• Microbiology and Biotechnology Letters
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• v.10 no.4
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• pp.283-288
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• 1982

Trypsin inhibitor produced by Streptomyces sp. was investigated its reactive characteristics against trypsin. The mode of inhibition against trypsin was mixed type of non-competitive and competitive with casein, and enzyme-inhibitor complex was formed rapidly. The inhibitory activity was increased by the addition of isoleucine and depressed by silver, mercuric or cupric ion. And when egg albumin or hemoglobin was used as substrate for trypsin, the inhibition ratio was changed. The inhibitor inhibited coagulation of blood of bovine.

• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1429-1432
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• 2015

To identify plant-derived cell signaling inhibitors with antifungal properties, a twocomponent screening system using both wild-type Cryptococcus neoformans and a calcineurin mutant was employed owing to their counter-regulatory actions on the Hog1 mitogenactivated protein kinase and calcineurin pathways. Of the 2,000 plant extracts evaluated, a single bioactive compound from M. obovata Thunb. was found to act specifically on the calcineurin pathway of C. neoformans. This compound was identified as magnoloside A, and had potent antifungal activities against various Cryptococcus strains with minimum inhibitory concentration values ranging from 1.0 to 4.0 μg/ml.

• Journal of Microbiology and Biotechnology
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• v.7 no.3
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• pp.167-173
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• 1997

Everted membrane vesicles of Helicobacter pylori were prepared and the membrane-resided ATPases were characterized. For comparison, Escherichia coli membrane ATPases and hog gastric mucosal H,K-ATPase were employed. ATPase assay revealed that the composite enzyme pool was relatively low in specific activities, below 1/10 times than that found in E. coli. According to their inhibitory specificities, most of the ATPase pool appeared to belong to the P-type ATPase, sensitive to vanadate but not to azide. The enzyme pool was extraordinarily resistant against treatment by N,N'-dicyclohexylcarbodiimide (DCCD). Certain monovalent cations, e.g., $K^+$ or $NH_4^{+}$ stimulated the whole enzyme pool only in the presence of $Mg^{2+}$. On the contrary, $Ni^{2+}$ and $Zn^{2+}$ increased enzyme activity rather effectively without the aid of $Mg^{2+}$. Under a defined condition employed, H. pylori cells could retain the membrane ATPase pool to the extent of $17{\%}$ at pH 3.2. Moreover, its activity was most stable in acidic conditions (pH 5.4-6.4). However, cytoplasmic or peripheral ATPase pools were hardly detected under acidity (below pH 4.6).

• Journal of Microbiology and Biotechnology
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• v.5 no.1
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• pp.36-40
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• 1995

Valistatin, a new inhibitor of aminopeptidase M(AP-M) was discovered in the culture broth of Streptomyces sp. SL20209 isolated from a soil sample. The inhibitor was purified by extraction with n-butanol and the various column chromatographies, and then isolated as whitish powder. The $^1 H-and ^1 H, ^1 H-COSY$ NMR studies, amino acid analysis, and fragmentation patterns by FAB-MS suggested the presence of one 3-amino-2-hydroxy-4-phenylbutanoic acid and two valine residues in the inhibitor. Thus, the structure of valistatin was determined as 3-amino-2-hydroxy-4-phenylbutanoyl-valyl-valine. Valistatin has the molecular formular $C_20H_31N_3 O_5$ (MW 394), and its $IC_50$ value against hog kidney AP-M was determined to be 3.12 $mu g/ml$.

• Journal of Advanced Navigation Technology
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• v.15 no.1
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• pp.91-96
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• 2011

In this paper, we design and implement a automatic control system of wireless sensor network based sprayer for hog barns. The proposed control system is driven by events from sensor nodes. It gathers various sensor readings such as temperature, humid, water level and water temperature, and controls the sprayer in real time by analyzing the sensor readings. Through experiments, we show that the proposed control system manages temperature and humidity steadily. Our proposed system enhances the existing system about 33% for temperature management and 37.3% for humidity management.

• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1377-1381
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• 2014

In order to discover and develop novel signaling inhibitors from plants, a screening system was established targeting the two-component system of Cryptococcus neoformans by using the wild type and a calcineurin mutant of C. neoformans, based on the counter-regulatory action of high-osmolarity glycerol (Hog1) mitogen-activated protein kinase and the calcineurin pathways in C. neoformans. Among 10,000 plant extracts, that from Harrisonia abyssinica Oliv. exhibited the most potent inhibitory activity against C. neoformans var. grubii H99 with fludioxonil. Bioassay-guided fractionation was used to isolate two bioactive compounds from H. abyssinica, and these compounds were identified as chebulagic acid and chebulanin using spectroscopic methods. These compounds specifically inhibited the calcineurin pathway in C. neoformans. Moreover, they exhibited potent antifungal activities against various human pathogenic fungi with minimum inhibitory concentrations ranging from 0.25 to over $64{\mu}g/ml$.

• 한국태양에너지학회:학술대회논문집
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• 2008.11a
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• pp.36-41
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• 2008

In this paper, we study The Effects of PV Cell's Electrical Characteristics for PV Module Application. Photovoltaic module consists of serially connected solar cell which has low open circuit voltage and high short circuit current characteristics. The whole current flow of PV module is restricted by lowest current of one solar cell. For the experiment, we make PV module composing the solar cells that have short circuit current difference of 0%, 1%, 3% and Random. The PV module exposed about 35days, its the maximum power drop ratio was 4.282% minimum and 6.657% maximum. And PV module of low current characteristics has electrical stress from other modules. The solar cell temperature of PV module was higher compared to PV cell. To prevent early degradation, it is need to have attention to PV cell selection.

• ETRI Journal
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• v.35 no.6
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• pp.1038-1047
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• 2013

Augmented reality (AR) is currently being applied actively to commercial products, and various types of intelligent AR systems combining both the Global Positioning System and computer-vision technologies are being developed and commercialized. This paper suggests an in-vehicle head-up display (HUD) system that is combined with AR technology. The proposed system recognizes driving-safety information and offers it to the driver. Unlike existing HUD systems, the system displays information registered to the driver's view and is developed for the robust recognition of obstacles under bad weather conditions. The system is composed of four modules: a ground obstacle detection module, an object decision module, an object recognition module, and a display module. The recognition ratio of the driving-safety information obtained by the proposed AR-HUD system is about 73%, and the system has a recognition speed of about 15 fps for both vehicles and pedestrians.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.3
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• pp.1700-1721
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• 2017

The problem of blocking artifacts is very common in block-based image and video compression, especially at very low bit rates. In this paper, we propose a post-processing method for JPEG-coded image deblocking via sparse representation and adaptive residual threshold. This method includes three steps. First, we obtain the dictionary by online dictionary learning and the compressed images. The dictionary is then modified by the histogram of oriented gradient (HOG) feature descriptor and K-means cluster. Second, an adaptive residual threshold for orthogonal matching pursuit (OMP) is proposed and used for sparse coding by combining blind image blocking assessment. At last, to take advantage of human visual system (HVS), the edge regions of the obtained deblocked image can be further modified by the edge regions of the compressed image. The experimental results show that our proposed method can keep the image more texture and edge information while reducing the image blocking artifacts.