• 생약학회지
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• 제16권2호
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• pp.73-80
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• 1985

Experimental studies were undertaken to investigate for the effect of Yijin-tang on the digestive system and isolated uterus of rats. Yijin-tang was composed of five crude drugs; Pinelliae Tuber, Aurantii nobilis Pericarpium, Hoelen, Glycyrrhizae Radix and Zingiberis Rhizoma. The results obtained from experiments with water soluble fraction extracted from Yijin-tang were as follows; Spontaneous motilities of isolated ileum of mice and rabbits were suppressed, and contraction of isolated ileum of mice and guinea-pigs induced acetylcholine, barium chloride and histamine were inhibited. The anticathartic action of mice induced by castor oil were significantly observed. The spontaneous motility of isolated uterus of rats was suppressed. Inhibitory effect of gastric juice secretion and antiulcerogenic action were significantly shown in Shay rats.

• Journal of Pharmaceutical Investigation
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• 제39권3호
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• pp.161-166
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• 2009

This study is to specify the criteria and testing methods of WHW extract which has a potency as a therapeutic agent for chronic renal failure. The determination of specifications of WHW extract is mostly important because of the quality assurance. Three batches of WHW extract were obtained by the extraction at $98^{\circ}$C for 3 hr using water from mixture of 15 herbal medicines including Codonopsis Pilosulae Radix, Salviae Radix, Pinelliae Rhizoma, Coptidis Rhizoma, Evodiae Fructus, Epimedii Herba, Rhei Rhizoma, Perillae Herba, Glycyrrhizae Radix, Artemixiae capillaris Herba, Alimatis Rhizoma, Hoelen, Atractylodies Rhizoma alba, Polyporus and Cinnamomi Ramulus, subsequently, vaccum-dried for 15 hr. The yield of WHW extracts was 24.53% on the average. The identification of each herbal medicine of WHW extract was performed by modification of Korean Pharmacopeia IX (KP IX). The assay of WHW extract was performed using standard such as berberine, icariin, glycyrrhizin, and cinnamic acid of indicative herbal medicines by modification of KP IX, too. As well as, paticle size classification test was carried out to indicate the boundary of particle size of WHW extract and the particle size of WHW extract more than 50% showed the 140 ${\mu}$m. Taken together, WHW extract could be prepared reproducibly and assurable if follows the presented extraction and drying steps and its specifications were satisfied with the indicated criteria.

• 대한본초학회지
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• 제29권6호
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• pp.157-163
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• 2014

Objectives : Gamisoyo-san complex prescription were made with Angelicae Gigantis Radix, Paeoniae Radix, Atractylodes rhizome white, Hoelen, Bupleuri Radix, Moutan Cortex Radicis, Gardeniae Fructus, Zingiberis Rhizoma Crudus, Menthae Herba. The purpose of this study was to research the whitening effect of the extract from Gamisoyo-san, which is one of the used herbal complex prescription. Methods : This study investigated inhibitory effect of Gamisoyo-san in tyrosinase activity. Cell viability were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Then, Gamisoyo-san measured reversed-transcription-PCR for mRNA expression using B16F10 mouse melanoma cells. Results : For whitening effects, the tyrosinase inhibition effect of extract was shown to 52.4% at $5,000{\mu}g/m{\ell}$ concentration. The cell viability on B16F10 melanoma cells of Gamisoyo-san extract showed higher than 75% at $1,000{\mu}g/m{\ell}$ concentration. In this study, an experiment was performed by setting the non-toxic concentration range of 50, 150, $250{\mu}g/m{\ell}$. The Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a positive control. The microphthalmia-associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), tyrosinase mRNA expression inhibitory by reverse transcription-PCR of Gamisoyo-san extract were decreased by 95.3%, 98.8%, 96.3% and 49.5% at $250{\mu}g/m{\ell}$ which the highest concentration. Conclusions : All these findings could verify that whitening effects of Gamisoyo-san extract by tyrosinase inhibitory activity and mRNA expression. The Gamisoyo-san could be used as material for functional cosmetics, such as skin whitening products.

• 생약학회지
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• 제18권2호
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• pp.127-135
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• 1987

In vitro sensitivity testing was performed for 21 kinds putative anticancer drugs selected from references and information. Cellular damage of P815 mastocytoma cells following exposure to water extracts of drugs was evaluated by colony formation assay. Highly effective drugs with more than 50% inhibition of colony formation were seven (Houttuyniae Herba, Sanguisorbae Radix, Nepetae Herba, Manitis Squama, Lonicerae Flos, Amomi Semen, Polyporus), though not more effective than BCNU. According to the results of $^3H-thymidine$ incorporation assay for determination of selective cytotoxicity, 3 of these drugs (Houttuyniae Herba, Polyporus, Manitis Squama) were found to be low cytotoxic to normal mouse lymphoid cells. These findings suggest that the above 3 drugs may be used for effective anticancer drugs in vivo.

• 대한한의학회지
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• 제20권2호
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• pp.121-127
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• 1999

Oriental herbal medicines were examined for mutagenicity in the reverse mutation test on Salmonella typimurium T A98/100 and chromosomal aberration test on cultured mammalian cells (Chinese hamster cell lines). The reverse mutation test was performed by a plate incorporation method with and without a metabolic activation system (S9 mix). The tested herbal medicines did not significantly increase revertible colonies on any of the test strains with and without a metabolic activation system (S9 mix) at concentrations of 1 mg/ml. In the chromosomal aberration test, most tested herbal medicines did not significantly increase the number of aberrant cells on any of the test strains with a metabolic activation system (S9 mix) at concentrations of 1 mg/ml, compared with the vehicle control. However. Ansu Semen significantly increased the number of aberrant cells without a metabolic activation system (S9 mix). Paeoniae Radix. Hoelen, Aurantii nobilis Pericarpium, Cnidii Rhizoma, Angeliacae gigantis Radix, Perillae Herba and Moutan Cortex Radicis slightly increased revertible colonies on any of the test strains with a metabolic activation system (S9 mix), These results indicate that most herbal medicines might be carefully used in obstetrics and gynecology, although they do not have the potent mutagenic potential under the present test conditions.

• 대한한의학방제학회지
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• 제21권2호
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• pp.53-62
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• 2013

Objectives : Diabetic nephropathy is associated with morbidity and mortality of diabetes mellitus patients. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis. Oryeong-san, Korean formula, is widely used for the treatment of nephrosis, edema, and uremia. Oryeong-san is composed of five herbs: Alismatis Rhizoma, Polyporus, Atractylodis Rhizoma Alba, Hoelen, and Cinnamomi Cortex. Methods : The present study was performed to investigate potent inhibitory effect of Oryeong-san on high glucose (HG)-induced rat mesangial cells (RMC) proliferation. Results : RMC proliferation under 25 mM glucose was significantly accelerated compared with 5.5 mM glucose, which was inhibited by Oryeong-san in dose dependent manner. Pre-treatment of Oryeong-san induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, Oryeong-san reduced HG-induced RMC proliferation by suppressed the mitogen-activated protein kinase (MAPK) phospholyration such as extracellular signal regulated kinase (ERK), Jun N-terminal Kinase (JNK), and p38. Oryeong-san significantly suppressed HG-induced ROS production. Conclusions : Oryeong-san consequently inhibited HG-induced mesangial cell proliferation through the inhibition of MAPK and ROS signaling pathway. These results suggest that Oryeong-san may be effective in the treatment of renal dysfunction leading to diabetic nephropathy.

• 한방재활의학과학회지
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• 제24권4호
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• pp.29-40
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• 2014

Objectives This study was designed to collect basic information about the herbal medicine used for dementia through analysis on Korean medicine prescriptions used in dissertations for a degree of Korean medicine related dementia (experimental study). Methods We searched dissertations in the National Assembly Library and oriental medicine advanced searching integrated system web DB by using keyword '치매' and 'dementia'. We reviewed and analyzed dissertations for the degree of Korean medicine, published from 2000 to 2013. Results As a result, We found 36 dissertations about Korean herbal medicines used for Dementia Yukmijihwang-tang, Chenwangbosim-dan were used much more than other prescriptions and Panax ginseng, Glycyrrhiza uralensis, Acorus gramineus, Polygala tenuifolia, Pachyma hoelen were used more than 10 times in these dissertations. Conclusions In relieving symptoms and the treatment of dementia, it is difficult to find tendency. Because the purpose and perspective of using each Korean medicine prescriptions and their herbs are various. However, as referred to outcome of preceding researches, Yukmijihwang-tang is considered as a potential dementia medicine.

• 대한의생명과학회지
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• 제11권3호
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• pp.417-420
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• 2005

To investigate the anti-obesitic effect of FD extract which consisted of Mori radicis Cortex, Hoelen, Pueraria radix, Schizonepetae spica, Carthami Flos, Bupleuri radix and Saposhnikoviae radix in high-fat diet-fed female lean Zucker rats. Obesity was induced by feeding high-lipid diet contained $3\%$ corn oil and $1\%$ cholesterol for 8 weeks, in which FD extract was added to the diet for treatment group. The rats fed on the high fat diet showed increased gain of body weights, leading to enhanced feed efficiency ratio. Moreover high-fat diet induced hepatic lipid peroxidation and hyperlipemia. The body weight, food efficiency ratio and lipid peroxidation in the liver was decreased as compared with the rats fed on the high fat diet by FD extract. With all such changes, the blood biochemistry about lipid was, if anything, risen. These results suggest that FD extract was supposed to have effective ingredients for improving obesity for controlling adipose tissue.

• Biomolecules & Therapeutics
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• 제12권3호
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• pp.151-156
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• 2004

This study was carried out to evaluate the putative anxiolytic-1ike effects of the aqueous extracts of Sanjoin-tang (SJIT) and its ingredients using the elevated plus maze (EPM) test in mice. SJIT consists of five herbs, namely, Zizyphi Spinosi Semen (roasted), Glycyrrhizae Radix, Cnidii Rhizoma, Anemarrhenae Rhizoma, and Hoelen. The aqueous extracts of SJIT and each herbal drug were orally administered to ICR mice, 1 hr before evaluating behavioral activity in the EPM test, respectively. Repeated treatments (for 3 days) of the aqueous extract of SJIT (400 mg/kg) significantly increased time-spend in the open arms and arms entries into the open arms in the EPM test. Zizyphi Spinosi Semen (400 mg/kg), an ingredient of SJIT, significantly increased timespent in the open arms and arm entries into the open arms (P < 0.05). However, the other ingredient of SJIT did not show any anxiolytic-like behaviors. In addition, the anxiolytic-like effects of Zizyphi Spinosi Semen were blocked by pindolol (lO mg/kg), a $5-HT_{1A}$ receptor antagonist. These results suggest that Zizyphi Spinosi Semen (roasted) as an ingredient of SJIT plays a crucial anxiolytic role, and it acts via the serotonergic nervous system.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2002년도 창립10주년기념 및 국립독성연구원 의약품동등성평가부서 신설기념 국재학술대회:생물학적 동등성과 의약품 개발 전략을 위한 국제심포지움
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• pp.219-219
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• 2002

The SPP003 is a mixture of water extract from Schizandrae Fructus, Poligoni multiflori Radix, Ginseng Radix Akba and Hoelen. The aim of this study was to investigate the anti-hyperglycemic effect of SPP003 in normal and streptozotocin (STZ)-induced diabetic rats, and to monitor the toxicity of SPP003. Oral glucose tolerance test (OGTT) was performed after oral administration of SPP003 100, 300, 600 and 900 mg/kg in normal rats. Blood glucese concentration was measured at -30 min (vehicle, SPP003 or tolbutamide 60 mg/kg, 0 min (glucose treatment), 60, 120 and 180 min. Rats were administerd STZ 65mg/kg (0.1M citrate buffer, pH 4.5) intraperitoneally to induce diabetes and administered vehicle, Spp003 (100, 300 and 600 mg/kg) or tolbutamide (60 mg/kg) orally once a day for 4 weeks. Blood glucose level was measured a 0, 4, 7, 14, 21 and 29 day after initial drug administration. A single oral toxicity of SPP003 was studied in Sprague-Dawley rats of both sexes. In this study, rats were administered with doses of 1, 2 and 5 g/kg of SPP003. In glucose tolerance test, SPP003 900 mg/kg markedly decreased glucose concentration at 1 hr after glucose treatment. Blood glucose levels were much higher in STZ-diabetic rats. These increases were significantly attenuated by SPP003 600 mg/kg. SPP003 did not show any signigicant toxicity. These findings suggest that SPP003 has hypoglycemic properties in STZ-diabetic rats.