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http://dx.doi.org/10.14374/HFS.2013.21.2.053

Oryeong-san Ameliorates High Glucose-induced Mesangial Cell Proliferation  

Yoon, Jung Joo (College of Oriental Medicine, Wonkwang University)
Lee, Yun Jung (College of Oriental Medicine, Wonkwang University)
Lee, So Min (College of Oriental Medicine, Wonkwang University)
Kim, Dae Hwan (College of Oriental Medicine, Wonkwang University)
Lee, Ho Sub (College of Oriental Medicine, Wonkwang University)
Kang, Dae Gill (College of Oriental Medicine, Wonkwang University)
Publication Information
Herbal Formula Science / v.21, no.2, 2013 , pp. 53-62 More about this Journal
Abstract
Objectives : Diabetic nephropathy is associated with morbidity and mortality of diabetes mellitus patients. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis. Oryeong-san, Korean formula, is widely used for the treatment of nephrosis, edema, and uremia. Oryeong-san is composed of five herbs: Alismatis Rhizoma, Polyporus, Atractylodis Rhizoma Alba, Hoelen, and Cinnamomi Cortex. Methods : The present study was performed to investigate potent inhibitory effect of Oryeong-san on high glucose (HG)-induced rat mesangial cells (RMC) proliferation. Results : RMC proliferation under 25 mM glucose was significantly accelerated compared with 5.5 mM glucose, which was inhibited by Oryeong-san in dose dependent manner. Pre-treatment of Oryeong-san induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, Oryeong-san reduced HG-induced RMC proliferation by suppressed the mitogen-activated protein kinase (MAPK) phospholyration such as extracellular signal regulated kinase (ERK), Jun N-terminal Kinase (JNK), and p38. Oryeong-san significantly suppressed HG-induced ROS production. Conclusions : Oryeong-san consequently inhibited HG-induced mesangial cell proliferation through the inhibition of MAPK and ROS signaling pathway. These results suggest that Oryeong-san may be effective in the treatment of renal dysfunction leading to diabetic nephropathy.
Keywords
Oryeong-san; Mesangial cell; Proliferation; Diabetic nephropathy;
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