• Journal of Microbiology
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• v.43 no.spc1
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• pp.110-117
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• 2005

Salmonella enterica serovar Typhimurium causes human gastroenteritis and a systemic typhoid-like infection in mice. A critical virulence determinant of Salmonella is the ability to invade mammalian cells. The expression of genes required for invasion is tightly regulated by environmental conditions and a variety of regulatory genes. The hilA regulator encodes an OmpR/ToxR family transcriptional regulator that activates the expression of invasion genes in response to both environmental and genetic regulatory factors. Work from several laboratories has highlighted that regulation of hilA expression is a key point for controlling expression of the invasive phenotype. A number of positive regulators of hilA expression have been identified including csrAB, sirA/barA, pstS, hilC/sirC/sprA, fis, and hilD. HilD, an AraC/XylS type transcriptional regulator, is of particular importance as a mutation in hilD results in a 14-fold decrease in chromosomal hilA::Tn5lacZY-080 expression and a 53-fold decrease in invasion of HEp-2 cells. It is believed that HilD directly regulates hilA expression as it has been shown to bind to hilA promoter sequences. In addition, our research group, and others, have identified genes (hilE, hha, pag, and lon) that negatively affect hilA transcription. HilE appears to be an important Salmonella-specific regulator that plays a critical role in inactivating hilA expression. Recent work in our lab has been directed at understanding how environmental signals that affect hilA expression may be processed through a hilE pathway to modulate expression of hilA and the invasive phenotype. The current understanding of this complex regulatory system is reviewed.

• Microbiology and Biotechnology Letters
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• v.40 no.1
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• pp.70-75
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• 2012

In Salmonella enterica, many genes encoded within Salmonella pathogenicity islands (SPI) 1 and 2 are required to cause a range of diseases in a variety of hosts. The SPI1-encoded regulator HilD activates both the SPI1 and 2 genes at different times during growth in Luria-Bertani (LB) media. In this study, the expression levels of hilD during growth in LB were investigated. The data suggest that hilD expression is induced in the early stationary phase and decreases in the late stationary phase, when sseA, an SPI2 gene, is maximally expressed. However, HilD could act as an activator of sseA expression in the late stationary phase despite being present at low levels. SseA expression was investigated in SPI1 regulator mutant strains, hilA, hilD and invF mutants. As expected, hilD mutation decreased sseA expression. However, we found that invF mutation caused a 1.5-fold increase in sseA expression in not only LB but also M9 minimal media, which is thought to resemble an intracellular environment. InvF overexpression restored sseA expression to wild-type levels in an invF mutant but did not cause an additional reduction in sseA expression. These results suggest that SPI1 controls SPI2 expression either positively or negatively.

• Proceedings of the KIPE Conference
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• 2013.11a
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• pp.37-38
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• 2013

This paper presents an intuitive and powerful way to study and design motor drive control. The control of induction motors, as most widely used machines, is discussed. Thanks to ultra low latency and high fidelity Hardware-in-the-Loop systems, different aspects of up-to-date drive regulation are examined. A power stage, comprised of a grid voltage source, a rectifier, a VSC inverter and an induction motor, is emulated on the HIL platform in real time. A digital signal controller is plugged into the interface board and connected to the HIL emulation platform, without any hardware modifications. For motor control and power electronics applications, a dedicated Texas Instruments TMS320F2808 DSP is chosen. The same controller can drive an emulation platform and a real device with no modifications. Current and speed control loop test results are presented and discussed.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.7
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• pp.949-955
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• 2018

Hardware-in-the-loop (HIL) simulation is a technique that can be employed for developing and testing complex real-time embedded systems. HIL simulation provides an effective platform for verifying power management system (PMS) performance of liquefied natural gas carriers, which are high value-added vessels such as offshore plants. However, HIL tests conducted by research institutes, including domestic shipyards, can be protracted. To address the said issue, this study proposes a field programmable gate array (FPGA) based PMS-HIL simulator that comprises a power supply, consumer, control console, and main switchboard. The proposed HIL simulation platform incorporated actual equipment data while conducting load sharing PMS tests. The proposed system was verified through symmetric, asymmetric, and fixed load sharing tests. The proposed system can thus potentially replace the standard factory acceptance tests. Furthermore, the proposed simulator can be helpful in developing additional systems for vessel automation and autonomous operation, including the development of energy management systems.

• The Journal of Korean Institute for Practical Engineering Education
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• v.3 no.2
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• pp.100-105
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• 2011

By adopting HIL(Hardware-in-the-Loop), component characteristics in vehicle environment can be obtained without implementing component in the vehicle. In this paper, when specific motor is adopted as traction motor in hybrid electric vehicle HIL implementation procedures are explained. In order to implement HIL method motor testing. vehicle performance simulator and load characteristic are explained. Vehicle controller used in simulator is directly uploaded in real controller. Especially as a load dynamometer actively controlled motor system is used without connecting conventional mechanical inertia. Motor characteristics are obtained using HIL implementation when test motor is used as a traction motor for parallel hybrid electric vehicle. Proposed method can be used as experimental equipment to educate driving characteristics of hybrid electric vehicle.

• Journal of Animal Science and Technology
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• v.64 no.6
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• pp.1063-1076
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• 2022

Two experiments were conducted to determine the effect of Hermetia illucens larvae (HIL) as protein and protease on growth performance, blood profiles, fecal microflora, and gas emission in growing pig. In experiment 1, the seventy-two crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial body weight (BW) of 27.98 ± 2.95 kg were randomly allotted to one of four dietary treatments (3 pigs per pen and 6 replicates pen per treatments). The experimental design was a 2 × 2 factorial arrangement of treatments evaluating two diets (Poultry offal diets and HIL diets) without or with supplementing protease. The poultry offal in basal diet has been replaced by HIL. In experiment 2, the four crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial BW of 28.2 ± 0.1 kg were individually accepted in stainless steel metabolism cages. The dietary treatments included: 1) PO- (PO-; poultry offal diet), 2) PO+ (PO- + 0.05% protease), 3) HIL- (3% PO of PO- diet was replacement 3% HIL), 4) HIL+ (HIL- + 0.05% protease). In experiment 1, From weeks 0 to 2, average daily gain (ADG) and feed efficiency (G:F) were significantly increased in the PO diet group compared with the HIL group. From weeks 2 to 4, ADG and G:F were higher for protease group than for non-protease group. At weeks 2 and 4, the PO diet group had lower blood urea nitrogen (BUN) levels than HIL diet group. In experiment 2, crude protein (CP) and nitrogen (N) retention were decreased by HIL diet at weeks 2 and 4. The fecal microflora and gas emission were not affected by HIL and protease. The HIL diet showed lower CP digestibility than PO diet and total essential amino acids digestibility tended to higher in PO diet than HIL diet. In summary, the present study revealed that replacement of the PO protein with the HIL protein and the additive of protease in growing pig diets during the overall experimental period had no negative effect.

• Journal of Astronomy and Space Sciences
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• v.26 no.1
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• pp.47-58
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• 2009

In this study, a Hardware-In-the-Loop (HIL) simulator using thrusters is developed to validate the spacecraft attitude system. To control the attitude of the simulator, eight cold gas thrusters are aligned with roll, pitch and yaw axis. Also linear actuators are applied to the HIL simulator for automatic mass balancing to compensate the center of mass offset from the center of rotation. The HIL simulator consists of an embedded computer (Onboard PC) for simulator system control, a wireless adapter for wireless network, a rate gyro sensor to measure 3-axis attitude of the simulator, an inclinometer to measure horizontal attitude, and a battery set to supply power for the simulator independently. For the performance test of the HIL simulator, a bang-bang controller and Pulse-Width Pulse-Frequency (PWPF) modulator are evaluated successfully. The maneuver of 68 deg. in yaw axis is tested for the comparison of the both controllers. The settling time of the bang -bang controller is faster than that of the PWPF modulator by six seconds in the experiment. The required fuel of the PWPF modulator is used as much as 51% of bang-bang controller in the experiment. Overall, the HIL simulator is appropriately developed to validate the control algorithms using thrusters.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1589-1590
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• 2007

본 논문에서는 대체에너지 발전시스템이 포함되는 복합발전시스템의 구성 및 운전 특성 등을 분석하고 종합적인 엔지니어링 기술 확보에 필요한 Hardware-in-the-loop(HIL) Simulator의 구조를 제시하며 이를 효율적으로 제어할 수 있는 운영 소프트웨어를 개발하였다. 개발된 HIL Simulator는 복합발전 시스템을 구성하는 서브시스템의 제어기를 설계하는데 유용하게 사용할 수 있다.

• Proceedings of the KIPE Conference
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• 2018.07a
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• pp.376-377
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• 2018

A hardware-in-the-loop (HIL) platform for a power management control of islanded DC microgrids is established. In order to avoid the complexity and high costs, a decentralized control based on the DC Bus Signaling (DBS) method is applied to the HIL system. The simulation results for the HIL microgrid platform have verified the effectiveness of power management strategy.

• Food Science and Preservation
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• v.17 no.2
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• pp.290-296
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• 2010

To develop anti-acidosis and anti-diabetes agentsfrom natural products, the inhibitory activities of Brazilian plant extracts against microbial $\alpha$-amylase and $\alpha$-glucosidase were evaluated. Among 100 different ethanol extracts tested, those of Acacia jurema Mart., Anacardium humile A. St.-Hil., Cedrela odorata L., and Guazuma ulmifolia Lam showed good inhibitoryactivities toward both enzymes. In addition, an extract of Plumeria drastica Mart. showed specific inhibition of $\alpha$-amylase, whereas that of Eugenia uniflora L. demonstrated strong inhibition of the enzyme. IC50 values of $\alpha$-amylase inhibition suggested that the extract of A. humile A. St.-Hil., which has been used as an anti-diabetes medicine in Brazil, had potent inhibitory activity. The IC50 for the A. humile A. St.-Hil. extract ($91.2{\mu}g/mL$) was similar to that of acarbose ($50.5{\mu}g/mL$). This activity of A. humile A. St.-Hil. was not reduced by heat or acid treatment. Moreover, treatment with HCl (0.01 M) for 1 h increased the inhibitory activity from 57.5% to 81.2%. Also, the extract did not cause hemolysis of human red blood cells at levels up to 1 mg/mL. The results indicate that the extract of A. humile A. St.-Hil. is potentially useful as an anti-acidosis and anti-diabetes agent.