• Preventive Nutrition and Food Science
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• v.16 no.2
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• pp.95-103
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• 2011

This study was performed to investigate the antioxidant mechanism of tomato wine with varying lycopene content in rats fed a high fat diet (HFD). Male Sprague-Dawley rats were randomly divided into five groups (n=10 per group) and fed an HFD (35% of total energy from fat) plus ethanol (7.2% of total energy from alcohol), tomato wine with varying lycopene content (0.425 mg%, 1.140 mg% or 2.045 mg% lycopene) or an isocaloric control diet for 6 weeks. Mice fed HFD plus ethanol significantly increased erythrocyte hydrogen peroxide and thiobarbituric acid reactive substances (TBARS) levels with increases in activities of erythrocyte antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) compared to pair-fed rats. Supplementation of tomato wine with varying lycopene content decreased ethanol-mediated increases of erythrocyte lipid peroxidation and antioxidant enzyme activities in HFD-fed rats, and tomato wine with higher lycopene appeared to be more effective. Tomato wine also dose-dependently lowered TBARS levels with decreased pro-oxidant enzyme, xanthine oxidase (XOD) activity in plasma of HFD-fed rats. In contrast to erythrocytes, the inhibitory effects of tomato wine on hepatic lipid peroxidation were linked to increased hepatic antioxidant enzymes (SOD and CAT) and alcohol metabolizing enzyme (alcohol dehydrogenase and aldehyde dehydrogenase) activities. There were no significant differences in hepatic XOD and cytochrome P450-2E1 activities among the groups. Together, our data suggest that tomato wine fortified with lycopene has the potential to protect against ethanol-induced oxidative stress via regulation of antioxidant or pro-oxidant enzymes and alcohol metabolizing enzyme activities in plasma, erythrocyte and liver.

• Microbiology and Biotechnology Letters
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• v.6 no.2
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• pp.51-57
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• 1978

The study was made of the cultural condition and physiological characteristics of the symbiotic pair of microorganisms, Cellulomonas flavigena and the second organism. It also contains the results of a taxonomical study of the second organism. The results obtained wers summarized as follows : 1) Cell yield of the mixed culture, Cellulomonas and the second organism, was higher than that of each pure culture in CM-Cellulose medium. 2) The taxonomical characteristics of the second organism revealed that it probably belonged to the genus Sporocytophaga because it had a gliding motility and microcyst. 3) Optimum pH of the mixed culture was found to be in the vicinity of 7.2, and optimum temperature of the cell growth in the mixed culture was observed to be in the vicinity of 30$^{\circ}C$. 4) It was found that the majority of the population during growth in the mixed culture consisted of Cellulomonas flavigena. 5) Cellulomonas flavigena required thiamine and biotin as growth factors but Sporocytophaga sp. had no requirement of vitamins. 6) Gulucose was not found in detectable amounts in the medium of Cellulomonas flavigena but it was traced in the mixture by thin layer chromatography. 7) Sixteen amino acids were analyzed from the cell protein of Cellulomonas flavigena by amino acid autoanalyzer. The amount of the leucine, valine and arginine was very high.

• Journal of Nutrition and Health
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• v.16 no.4
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• pp.263-272
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• 1983

Effects of dietary protein qualify, energy restriction, and subsequent rehabilitation were studied in the weanling rats. Rats were devided into the six experimental diet groups. Rats in AC (casein- containing diet ), AS (soyprotein-containing diet), and AG(gluten-containing diet) group consumed their diets ad libitum, and rats in PC, PS, PG groups were pair-fed in 60% of the of amounts eaten by corresponding rats in the AC, AS, AG groups, respectively. After 3 weeks of nutritional restriction, all rats were switched to the AC diet for 14 days. At the end of restriction period, body weight, weight gain, food intake, and FER were higher in casein- fed rats than other groups of rats. The differences were, however, disappeared with rehabilitation diet, except body weights which were not fully recovered until the end of experiment. The weights of liver, gastrocnemius muscle, kidney, small intestine, spleen, and lung were the highest in the casein group, and the lowest in gluten group at the end of restriction period. The recovery with rehabilitation diet were differ in different organs. The muscle and liver seemed to be the most affected organs by dietary protein quality and energy restriction. There were no differences in brain weights among the experimental groups during the restriction period, however, after 2 weeks of rehabilitation, rats in AC group showed lower brain weight compared to AC and AS groups, and the brain weight of PC group was lower than AC group. Protein contents in liver and muscle showed the same trends as their weights. Gluten-fed rats showed low serum protein concentration, but recovered fully with rehabilitation diet for 3 days.

• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.650-655
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• 1998

The chromosomal DNA fragment from Phaffia rhodozyma CBS 6938 which is able to autonomously replicate in the yeast Saccharomyces cerevisiae was cloned on an integrative URA3 plasmid. Its minimal fragment exhibiting autonomously replicating activiy in the S. cerevisiae gave a higher frequency transformation efficiency than that found for centromere-based plasmid, and enabled extrachromosoma1ly stable transmission of the plasmids in one copy per yeast cell under non-selective culture condition. The 836-bp DNA element lacked an ORF and did not contain any acceptable match to an ARS core consensus. Sequence analysis, however, displayed a cluster of three hairpin-Ioop-sequences with individual $\triangle {G_{25}}^{\circ}C$ free energy value of -10.0, -17.5, and -17.0 kcal. $mor^{-l}$as well as a 9-bp sequence with two base pair mismatches to the S. cerevisiae/E. coli gyrase-binding site. This 836-bp sequence also included one 7-bp sequence analogous to the core consensus of centromeric DNA element III (CDEIII) of S. cerevisiae, but CDEIII-like 7 bp sequence alone did not give a replicative function in this yeast.

• Korean Journal of Food Science and Technology
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• v.47 no.5
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• pp.608-614
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• 2015

Four Korean commercial rice wines of diverse sensory properties were hedonically rated by 41 consumers. Each rice wine was paired with the selection from six widely popular compatible foods such as kimchi-jjigae, dotori-muk muchim, tteok-galbi, beoseot-jeongol, satae pyeonyuk, and haemul pajeon, and consumers were asked to rate the ideal match of each pair of four rice wines and six compatible foods by using a structured, 12-cm 'just right' line scale. Hedonic ratings of wines were additionally measured after intake of the food-wine pairs. Flavor matches of rice wines with strong ginseng, medicinal, and earthy flavor (JK) or with intense grain and sweet flavor (HS) were significantly higher compared to wines with fruity (SS) or mild-balanced (BS) flavor. The preference for HS wine, which ideally matched satae pyeonyuk, increased most significantly after intake of satae pyeonyuk, while the SS wine that matched ideally with kimchi jjigae did not show any significant increase in preference after intake of kimchi jjigae. Matching wines with food does not negatively affect the preference for the wine; rather, intake of a wine-food pairing increases the overall preference for rice wine.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1375-1378
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• 2005

The objective of this study was to investigate polymorphisms of BMPRIB (bone morphogenetic protein type IB receptor) gene and its effect on litter size traits in sheep. Three populations including 101 Small Tailed Han sheep, 79 Poll Dorset and 81 hybrids (Poll Dorset${\times}$Small Tailed Han sheep) were used to detect the polymorphisms in exon 6 region of sheep BMPRIB gene. A fragment of approximately 190bp was amplified by one pair of primers, the polymorphism was revealed from the analysis of three populations by the technique of PCR -SSCP, and a mutation from A to G at 746 of the coding region was confirmed by sequencing in several individual. Statistical results indicated the distribution of allele B (with a A$\longrightarrow$G mutation) and A (without mutation) or genotype AA, AB and BB frequencies differed in three populations. BB genotype (44.55%) and B allele (66.34%) frequencies of Small Tailed Han sheep were higher than those of the others. Analysis of variance showed that the polymorphism of BMPRIB gene was associated with positive effect on litter size traits. The means of genotype BB and AB were about 1.04 and 0.74 more than genotype AA for litter size (p<0.05). Analysis of BMPRIB genotype effects on litter size in three populations indicates the existence of genotype BB or B allele increases the litter size. It suggested that the polymorphism in exon 6 (at 746 in the coding region) of sheep BMPRIB gene may be used as a marker for early selection of prolificacy in sheep.

• Journal of The Korean Society of Agricultural Engineers
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• v.47 no.3
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• pp.59-68
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• 2005

The rainfall-runoff potential of Jangseong reservoir watershed was studied based on SCS (Soil Conservation Service, which is now the NRCS, Natural Resources Conservation Service, USDA) runoff curve number (CN) technique. Precipitation and reservoir operation data had been collected. The rainfall-runoff pairs from the watershed for ten years was estimated using reservoir water balance analysis using reservoir operation records. The maximum retention, S, for each storm event from rainfall-runoff pair was estimated for selected storm events. The estimated S values were arranged in descending order, then its probability distribution was determined as log-normal distribution, and associated CNs were found about probability levels of Pr=0.1, 0.5, and 0.9, respectively. A subwatershed that has the similar portions of land use categories to the whole watershed of Jangseong reservoir was selected and hydrologic monitoring was conducted. CNs for subwatershed were determined using observed data. CNs determined from observed rainfall-runoff data and reservoir water balance analysis were compared to the suggested CNs by the method of SCS-NEH4. The $CN_{II}$ measured and estimated from water balance analysis in this study were 78.0 and 78.1, respectively. However, the $CN_{II}$, which was determined based on hydrologic soil group, land use, was 67.2 indicating that actual runoff potential of Jangseong reservoir watershed is higher than that evaluated by SCS-NEH4 method. The results showed that watershed runoff potential for large scale agricultural reservoirs needs to be examined for efficient management of water resources and flood prevention.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1090-1097
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• 2007

Genomic analysis of Thermococcus sp. NA1 revealed the presence of a 3,927-base-pair (bp) family B-type DNA polymerase gene, TNA1_pol. TNA1_pol, without its intein, was overexpressed in Escherichia coli, purified using metal affinity chromatography, and characterized. TNA1_pol activity was optimal at pH 7.5 and $75^{\circ}C$. TNA1_pol was highly thermostable, with a half-life of 3.5h at $100^{\circ}C$ and 12.5h at $95^{\circ}C$. Polymerase chain reaction parameters of TNA1_pol such as error-rate, processivity, and extension rate were measured in comparison with rTaq, Pfu, and KOD DNA polymerases. TNA1_pol averaged one incorrect bp every 4.45 kilobases (kb), and had a processivity of 150 nucleotides (nt) and an extension rate of 60 bases/s. Thus, TNA1_pol has a much faster elongation rate than Pfu DNA polymerase with 7-fold higher fidelity than that of rTaq.

• Korean Journal of Remote Sensing
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• v.33 no.1
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• pp.69-77
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• 2017

S The need for an automated geo-positioning approach for near real-time results and to boost cost-effectiveness has become increasingly urgent. Following this trend, a new approach to automatically compensate for the bias of the rational function model (RFM) was proposed. The core idea of this approach is to remove the bias of RFM only using tie points, which are corrected by matching with the digital elevation model (DEM) without any additional ground control points (GCPs). However, there has to be a additional evaluation according to the quality of DEM because DEM is used as a core element in this approach. To address this issue, this paper compared the quality effects of DEM in the conduct of the this approach using the Shuttle Radar Topographic Mission (SRTM) DEM with the spatial resolution of 90m. and the National Geographic Information Institute (NGII) DEM with the spatial resolution of 5m. One KOMPSAT-2 stereo-pair image acquired at Busan, Korea was used as experimental data. The accuracy was compared to 29 check points acquired by GPS surveying. After bias-compensation using the two DEMs, the Root Mean Square (RMS) errors were less than 6 m in all coordinate components. When SRTM DEM was used, the RMSE vector was about 11.2m. On the other hand, when NGII DEM was used, the RMSE vector was about 7.8 m. The experimental results showed that automated geo-positioning approach can be accomplished more effectively by using NGII DEM with higher resolution than SRTM DEM.

• Proceedings of the Korea Society for Simulation Conference
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• 2001.10a
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• pp.321-324
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• 2001

The robot soccer simulation game is a dynamic multi-agent environment. In this paper we suggest a new reinforcement learning approach to each agent's dynamic positioning in such dynamic environment. Reinforcement Beaming is the machine learning in which an agent learns from indirect, delayed reward an optimal policy to choose sequences of actions that produce the greatest cumulative reward. Therefore the reinforcement loaming is different from supervised teaming in the sense that there is no presentation of input-output pairs as training examples. Furthermore, model-free reinforcement loaming algorithms like Q-learning do not require defining or loaming any models of the surrounding environment. Nevertheless it can learn the optimal policy if the agent can visit every state-action pair infinitely. However, the biggest problem of monolithic reinforcement learning is that its straightforward applications do not successfully scale up to more complex environments due to the intractable large space of states. In order to address this problem, we suggest Adaptive Mediation-based Modular Q-Learning(AMMQL) as an improvement of the existing Modular Q-Learning(MQL). While simple modular Q-learning combines the results from each learning module in a fixed way, AMMQL combines them in a more flexible way by assigning different weight to each module according to its contribution to rewards. Therefore in addition to resolving the problem of large state space effectively, AMMQL can show higher adaptability to environmental changes than pure MQL. This paper introduces the concept of AMMQL and presents details of its application into dynamic positioning of robot soccer agents.