• Journal of Korean Neurosurgical Society
• /
• v.64 no.4
• /
• pp.505-513
• /
• 2021

Objective : The adaptive immune response following subarachnoid hemorrhage (SAH) is not well understood. We evaluated and compared the T cell receptor (TCR) immune repertoire of good-grade and poor-grade SAH patients to elucidate the T cell immunology after ictus. Methods : Peripheral blood from six SAH patients was collected at two different times, admission and at the 7-day follow-up. Composition and variation of the TCR β-chain (TCRB) complimentary determining regions (CDR) 3 repertoire was examined using high-throughput sequencing; the analysis was based on sampling time and disease severity (good vs. poor-grade SAH). Results : Clonality at admission and follow-up were 0.059 (0.037-0.038) and 0.027 (0.014-0.082) (median, 25th-75th percentile). Poor-grade SAH (0.025 [0.011-0.038]) was associated with significantly lower clonality than good-grade SAH (0.095 [0.079-0.101]). Poor-grade SAH patients had higher diversity scores than good-grade SAH patients. CDR length was shorter in good-grade SAH vs. poor-grade SAH. Differences in clonotype distribution were more prominent in TCRBV gene segments than TCRBJ segments. TCRBV19-01/TCRBJ02-04 and TCRBV28-01/TCRBJ02-04 were the most increased and the most decreased V-J pairs in the 7-day follow-up compared to admission in good-grade SAH. The most increased and decreased V-J pairs in poor-grade SAH patients were TCRBV28-01/TCRBJ02-06 and TCRBV30-01/TCRBJ02-04, respectively. Conclusion : The TCRB repertoire is dynamic in nature following SAH. TCRB repertoire may facilitate our understanding of adaptive immune response according to SAH severity.

• The Journal of Society for e-Business Studies
• /
• v.26 no.4
• /
• pp.1-13
• /
• 2021

With the development of big data-related technologies, it has become possible to process vast amounts of data that could not be processed before. Accordingly, the establishment of an automated data selection and fusion process for the realization of big data-based services has become a necessity, not an option. In this paper, we propose an automation technique to create meaningful new information by fusing datasets containing spatial information. Firstly, the given datasets are embedded by using the Node2Vec model and the keywords of each dataset. Then, the semantic similarities among all of datasets are obtained by calculating the cosine similarity for the embedding vector of each pair of datasets. In addition, a person intervenes to select some candidate datasets with one or more spatial identifiers from among dataset pairs with a relatively higher similarity, and fuses the dataset pairs to visualize them. Through such semi-automatic data fusion processes, we show that significant fused information that cannot be obtained with a single dataset can be generated.

• Journal of Life Science
• /
• v.18 no.7
• /
• pp.924-930
• /
• 2008

Community structure refers to the number of species in a community and the pattern of distribution of individuals among those species. The purpose of this paper was to describe a statistical analysis for detecting a ecological biodiversity which is valid even though the assumption at the different sampling points is not violated spatial randomness of species. Counts and cover were determined from 10 ($20\;m{\times}20\;m$) plots in five sites of the Oncheon River which is located in Busan, Korea. Total 95 taxa (85 species, 9 varieties, and one form) were identified and measured in edge sides of this river. These were a total of present in the five sites. Overall across the fragments, mean number of species per plot differed significantly among the five sites (F=7.75, p<0.01). Shannon-Wiener functions differed significantly among plots (F=4.12, p<0.05), with the St. 1 having significantly higher value (2.380) than the others (2.206 for St. 2, 2.116 for St. 3, 2.069 for St. 4, and 0.637 for St. 5). The richness indices, R1 decreased from the upper stream of the Oncheon River to the lower stream. We used a novel way of representing community structure to show that abundance within closely related pairs of co-occurring species in the Oncheon River. The differences between the distributions for of congeners and pairs of non-congeners showed at the largest difference of the cumulative fractions of the data sets (x=0.85).

• Journal of Korean Society of Forest Science
• /
• v.95 no.4
• /
• pp.435-442
• /
• 2006

A total of 167 peculiar haplotypes confirmed from 28 cpSR variants that were observed in 19 populations of Japanese red pine in Korea through cpSSR marker analysis. Thirteen individuals that showed identical haplotype dispersed evenly in 10 populations, and the average number of effective haplotype within population was 13.37. Estimate of genetic diversity (He) was 0.987 on the basis of cpSSR haplotype variants that was equivalent to or higher than the estimates reported in other studies on some forest tree species. Estimation of genetic diversity (S.I.) on the basis of cpSSR variants composing each haplotype revealed the highest estimate of 1.109 for the population of Gangwon-Yeongwol and the lowest estimate of 0.411 for the population of Gyeongbuk Mungyeong with the average of 0.887. Most of observed cpSSR variants appeared to exist commonly in 19 populations (97.62%), and genetic differentiation of cpSSR variants among populations was turned out to be weak (${\Phi}_{ST}=0.024$). Relatively fast rate of mutation of cpSSR marker might be a major cause for such weak population differentiation. There was no identical haplotype shared between 39 population pairs of 173 pair-wise population pairs. Estimation of genetic distance among 19 populations on the basis of population pairs was also impossible, that might be resulted from restricted migration among 19 populations. Considering the observed distribution patterns of cpSSR variants in addition to the previous studies on I-SSR variants, informations on the present geographic location and genetic status of populations should be considered together for effective sustainable management of the genetic resources of Japanese red pine in Korea.

• Journal of Korean Society of Transportation
• /
• v.31 no.2
• /
• pp.60-68
• /
• 2013

This study developed plural gravity models and their application method in order to increase the accuracy of trip distribution estimation. The developed method initially involves utilizing the coefficient of determination ($R^2$) to set the target level. Afterwards, the gravity model is created, and if the gravity model's coefficient of determination is satisfactory in regards to the target level, the model creation is complete and future trip distribution estimation is calculated. If the coefficient of determination is not on par with the target level, the zone pair with the largest standardized residual is removed from the model until the target level is obtained. In respect to the model, the removed zone pairs are divided into positive(+) and negative(-) sides. In each of these sides, gravity models are made until the target level is reached. If there are no more zone pairs to remove, the model making process concludes, and future trip distribution estimation is calculated. The newly developed plural gravity model and application method was adopted for 42 zone pairs as a case study. The existing method of utilizing only one gravity model exhibited a coefficient of determination value ($R^2$) of 51.3%, however, the newly developed method produced three gravity models, and exhibited a coefficient of determination value ($R^2$) of over 90%. Also, the accuracy of the future trip distribution estimation was found to be higher than the existing method.

• Journal of Plant Biology
• /
• v.35 no.3
• /
• pp.237-245
• /
• 1992

To understand the properties of the cauliflower mosaic virus (CaMV) 35S promoter and the effect of the deleted maize alcohol dehydrogenase I-S (Adhl-S) intron 1 on the expression of the CaMV $35S{\beta}-glucuronidase$ (GUS) gene in potato (Solanum tuberosum L. cv. Superior), we constructed a chimeric gene and transferred it into potato with Agrobacterium tumefaciens mediated method. The pLS201, a gene transfer vector of 17.7 kilobase pairs, was composed of the CaMV 35S promoter, the 249 base pairs of deleted maize Adhl-S intron 1, the GUS reporter gene, and the kanamycin resistance gene as a selectable marker for transformation. The GUS activity was examined by histochemical and spectrophotometric assay in transformed potato plants. The GUS activity was found primarily around the vascular tissue cells in stem and root. In the spectorophotometric assay, the level of GUS activity of transgenic potato transformed with CaMV 35S/249 bp of intron 1 fragment-GUS (pLS201) was compared with that of potato transformed with CaMV 35S-GUS (pBI121). The quantitative spectrophotometric assay showed that the level of GUS activity in potato transformed with pLS201 was higher in leaf, stem and root by 30-, 34- and 42-fold, respectively than those in potato transformed with pBI121. This results indicate that the inclusion of the deleted maize Adhl-S intron 1 resulted in increament of the GUS gene expression in transgenic potato.potato.

• Journal of the Korea Society of Computer and Information
• /
• v.15 no.12
• /
• pp.197-207
• /
• 2010

Using a variety of data-mining methods on high-throughput cDNA microarray data, the level of gene expression in two different tissues can be compared, and DEG(Differentially Expressed Gene) genes in between normal cell and tumor cell can be detected. Diagnosis can be made with these genes, and also treatment strategy can be determined according to the cancer stages. Existing cancer classification methods using machine learning select the marker genes which are differential expressed in normal and tumor samples, and build a classifier using those marker genes. However, in addition to the differences in gene expression levels, the difference in gene-gene correlations between two conditions could be a good marker in disease diagnosis. In this study, we identify gene pairs with a big correlation difference in two sets of samples, build gene classification modules using these gene pairs. This cancer classification method using gene modules achieves higher accuracy than current methods. The implementing clinical kit can be considered since the number of genes in classification module is small. For future study, Authors plan to identify novel cancer-related genes with functionality analysis on the genes in a classification module through GO(Gene Ontology) enrichment validation, and to extend the classification module into gene regulatory networks.

• Physical Therapy Korea
• /
• v.12 no.3
• /
• pp.39-45
• /
• 2005

Tracking is an experimental paradigm that can be used to study information processing in continuous movements involving accurate, ongoing control of motor performance. The purpose of this study was to identify the effects of knee tracking performance. Six patients with hemiplegia and six age-matched controls participated in the study. The tracking test was administrated. It was composed with regular ranges of $0^{\circ}C$ to $40^{\circ}C$ and randomized range .2 to .4 Hz. Using the Mann-Whitney U test, a comparison was made between subjects who had suffered from stroke and subjects who were well coordinated. The Wilcoxon Matched Pairs Signed Ranks Test was used to compare and analyze the paretic and nonparetic sides of the stroke patients. The results of study were as follows: accuracy index of the tracking test was significantly higher on the control side than paretic and nonparetic sides. Accuracy index scores were significantly higher for nonparetic sides with stroke compared with paretic sides with stroke. This study shows tracking is impaired in paretic and nonparetic knee of subjects with stroke.

• Phonetics and Speech Sciences
• /
• v.4 no.3
• /
• pp.25-30
• /
• 2012

This study investigated whether native Korean listeners could use visual cues for the identification of the English consonants /b/ and /v/. Both auditory and audiovisual tokens of word minimal pairs in which the target phonemes were located in word-initial or word-medial position were used. Participants were instructed to decide which consonant they heard in $2{\times}2$ conditions: cue (audio-only, audiovisual) and location (word-initial, word-medial). Mean identification scores were significantly higher for audiovisual than audio-only condition and for word-initial than word-medial condition. Also, according to signal detection theory, sensitivity, d', and response bias, c were calculated based on both hit rates and false alarm rates. The measures showed that the higher identification rate in the audiovisual condition was related with an increase in sensitivity. There were no significant differences in response bias measures across conditions. This result suggests that native Korean speakers can use visual cues while identifying confusing non-native phonemic contrasts. Visual cues can enhance non-native speech perception.

• Journal of Korean Academy of Nursing
• /
• v.30 no.5
• /
• pp.1133-1144
• /
• 2000

This study was conducted to evaluate the effects of the program for the autistic children which was designed to promote mother-child attachment. The subjects of this study were consisted of 11 pairs of mother and child( 7 for participant group:PG, 4 for non-participant group: NPG), who were diagnosed as Pervasive Developmental Disorder. The intervention was consisted with play activity centered mother-child interaction, educational activity for mothers, lecture, video-feedback, and supports. The main data were collected by video-taping and analyzed by Wilcoxon Rank Sign Test, and Content Analysis. The results obtained were as follows, 1. Before the program, there were no significant group differences on the children's and the mother's characteristics. After the program, total score on the attachment of PG was higher than that of NPG, but not significant. Only proximity-seeking behaviors and contact-maintaining behaviors were higher significantly(p<.05). Then the characteristics on contact- maintaining behaviors of PG were lasting longer and reciprocal than those of NPG. 2. After the program, the score on mother's nurturing behavior of PG was significantly more increased. The mother's behaviors to her child of PG became more child- centered, positively responsive, expressive supportively. But there were pretty big individual difference. It can be concluded that Mother-child Attachment Promotion Program is effective. Thus it can be recommended to be a early intervention model for autistic children.