• 한국가금학회지
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• 제44권3호
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• pp.199-209
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• 2017

Mitogen-activated protein kinase (MAPK) signaling pathways play a key role in innate immunity, inflammation, cell proliferation, cell differentiation, and cell death. The main objective of this study was to investigate the expression level of candidate MAPK pathway genes in the intestinal mucosal layer of two genetically disparate chicken lines (Marek's disease-resistant line 6.3 and Marek's disease-susceptible line 7.2) induced with necrotic enteritis (NE). Using high-throughput RNA sequencing, we investigated 178 MAPK signaling pathway related genes that were significantly and differentially expressed between the intestinal mucosal layers of the NE-afflicted and control chickens. In total, 15 MAPK pathway genes were further measured by quantitative real-time PCR(qRT-PCR) and the results were consistent with the RNA-sequencing data. All 178 identified genes were annotated through Gene Ontology and mapped onto the KEGG chicken MAPK signaling pathway. Several key genes of the MAPK pathway, ERK1/2, JNK1-3, p38 MAPK, MAP2K1-4, $NF-{\kappa}B1/2$, c-Fos, AP-1, Jun-D, and Jun, were differentially expressed in the two chicken lines. Therefore, we believe that RNA sequencing and qRT-PCR analysis provide resourceful information for future studies on MAPK signaling of genetically disparate chicken lines in response to pathogens.

• Journal of the Korean Wood Science and Technology
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• 제26권1호
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• pp.97-105
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• 1998

This study examined the combined using effects of urea-formaldehyde (UF) resin and emulsifiable methylene diphyenyl diisocyanate (EMDI) resin to overcome performance limit of three-layer particleboards commonly made by UF resin. Two adhesive adding methods were applied with three types of resin combination system to each layer of particleboards. The one was simultaneously spreading method with emulsified compound resin (UF and EMDI) while the other was separately spreading method with unemulsified EMDI resin after UF resin spreading. The performance of particleboards bonded with 2% EMDI resin to the inner layers(IL) were similar to that of controls bonded with 8% UF resin. In the case of the emulsified compound resin application to the all layers of particleboards, there were marked reinforcing effects of EMDI resin, although a small amount of EMDI resin was mixed with UF resin. Especially bending MOR after 24 hours cold water-immersion and thickness swelling after 2 hours hot water-immersion of compound resin-bonded particleboards were remarkably different from those of pure UF resin-bonded particleboards. It was found that separately spreading method with unemulsified EMDI resin was more effective than simultaneously spreading method with emulsified compound resin to sustain the internal bond strength of particleboards after 24 hours cold water-immersion. In the resin combination systems to outer layers/inner layers of particleboards, water resistance and strength properties were superior in order of UF+EMDI/UF+EMDI > UF/UF+EMDI > UF/UF. And water resistance of particleboards was greatly dependent upon EMDI resin level in any adhesive adding method.

• International Journal of Oral Biology
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• 제40권3호
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• pp.111-116
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• 2015

To determine the effect of diabetes on root resorption in periodontitis, we investigated odontoclast formation and root resorption in diabetic rats with periodontitis. Odontoclast formation was observed in three groups of F344 rats: Controls (C) were normal rats without diabetes or periodontitis; the periodontitis (P) group had mandibular first molars to be ligatured; the periodontitis with diabetes (PD) group was intravenously administered streptozotocin (50 mg/kg) to induce diabetes and had mandibular first molars to be ligatured. On days 3, 10, and 20 after ligature, tumor necrosis factor (TNF)-${\alpha}$ and receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) expression, odontoclast formation, and root resorption areas were evaluated by immunohistochemistry, tartrate-resistant acid phosphatase staining, and hematoxylin and eosin staining, respectively. The PD group showed frequent urination, weight loss, and hyperglycemia. Numbers of TNF-${\alpha}$- and RANKL-positive cells were higher in the P and PD groups than in the C group. It was more prevalent in PD group on day 3. Odontoclast formation was greater in the P and PD groups than in the C group on days 3 and 10, then decreased to same level as the C group by day 20. Root resorption in the PD and P groups showed increases on days 3 and 10, respectively, compared to the C group. These results suggest that diabetes may transiently increase root resorption on day 3 with high expression of TNF-${\alpha}$ and RANKL after periodontitis induction. This study could aid the understanding of root resorption in diabetic patients with periodontitis.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.370-378
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• 2003

MFD (Make up Film Deterioration) is a gradual deterioration of applied make up and is a common problem experienced by most foundation users. Our investigation revealed that for 64% of all make up users MFD is their greatest consern is using foundations. Known that the primary cause of MFD is sebum secretion. We observed that the length of time prior to onset of MFD in people who produce high level of sebum varies significantly from person to person. This suggests that other factors besides quantity of sebum production can affect MFD. Control over this factor would, we believe, be key to developing longer-lasting makeups. We studied the relationship between MFD and skin surface conditions. Our study revealed that furrows on the skin surface affect MFD significantly. Sebum reaches the skin surface from sebaceous glands and flows along furrow on the skin. If there are many deep furrows, it takes longer for sebum to overflow. But if the furrows are few or shallow, sebum quickly overflows and spreads over the skin surface where it can degrade the make up film. Therefore even when the volume of sebum produced is the same, the rate of MFD will be different depending on the number and shape of the furrows. A longer-lasting foundation could be produced by matching personal skin condition, but this would be very difficult because individual variations in texture are very large. Therefore we approached the problem by attempting to impose sebum resistance in under make up and foundation. We have developed two new materials and make up products based on our theory. A new fluoroalkyl acrylate-methacrylates copolymer designed for incorporation in under make up is extremely sebum resistant and sweat proof. Another new acrylate polymer designed for inclusion in foundation absorbs sebum and changes to a solid. Usage tests confirm it is possible to reduce MFD by using under make up and foundation which incorporate our new materials to cover where skin furrows are few or shallow

• Journal of Ginseng Research
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• 제44권2호
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• pp.332-340
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• 2020

Background: The valuable medicinal plant Panax ginseng has high pharmaceutical efficacy because it produces ginsenosides. However, its yields decline because of a root-rot disease caused by Ilyonectria mors-panacis. Because species within Ilyonectria showed variable aggressiveness by altering ginsenoside concentrations in inoculated plants, we investigated how such infections might regulate the biosynthesis of ginsenosides and their related signaling molecules. Methods: Two-year-old ginseng seedlings were treated with I. mors-panacis and I. robusta. Roots from infected and pathogen-free plants were harvested at 4 and 16 days after inoculation. We then examined levels or/and expression of genes of ginsenosides, salicylic acid (SA), jasmonic acid (JA), and reactive oxygen species (ROS). We also checked the susceptibility of those pathogens to ROS. Results: Ginsenoside biosynthesis was significantly suppressed and increased in response to infection by I. mors-panacis and I. robusta, respectively. Regulation of JA was significantly higher in I. robusta-infected roots, while levels of SA and ROS were significantly higher in I. mors-panacis-infected roots. Catalase activity was significantly higher in I. robusta-infected roots followed in order by mock roots and those infected by I. mors-panacis. Moreover, I. mors-panacis was resistant to ROS compared with I. robusta. Conclusion: Infection by the weakly aggressive I. robusta led to the upregulation of ginsenoside production and biosynthesis, probably because only a low level of ROS was induced. In contrast, the more aggressive I. mors-panacis suppressed ginsenoside biosynthesis, probably because of higher ROS levels and subsequent induction of programmed cell death pathways. Furthermore, I. mors-panacis may have increased its virulence by resisting the cytotoxicity of ROS.

• Asian-Australasian Journal of Animal Sciences
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• 제32권10호
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• pp.1580-1590
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• 2019

Objective: The aims of this study were to select one strain of Lactobacillus plantarum (L. plantarum) for a potential indigenous safe starter culture with low level antibiotic resistant and low biogenic amine production and evaluate its effect on biogenic amines reduction in Moo som. Methods: Three strains of indigenous L. plantarum starter culture (KL101, KL102, and KL103) were selected based on their safety including antibiotic resistance and decarboxylase activity, and fermentation property as compared with a commercial starter culture (L. plantarum TISIR543). Subsequently, the effect of the selected indigenous safe starter culture on biogenic amines formation during Moo som fermentation was studied. Results: KL102 and TISIR 543 were susceptible to penicillin G, tetracycline, chloramphenicol, erythromycin, gentamycin, streptomycin, vancomycin, ciprofloxacin and trimethoprim (MIC90 ranging from 0.25 to $4{\mu}g/mL$). All strains were negative amino acid-decarboxylase for lysis of biogenic amines in screening medium. For fermentation in Moo som broth, a relatively high maximum growth rate of KL102 and TISIR543 resulted in a generation time than in the other strains (p<0.05). These strain counts were constant during the end of fermentation. Similarly, KL102 or TISIR543 addition supported increases of lactic acid bacterial count and total acidity in Moo som fermentation. For biogenic amine reduction, tyramine, putrescine, histamine and spermine contents in Moo som decreased significantly by the addition KL102 during 1 d of fermentation (p<0.05). In final product, histamine, spermine and tryptamine contents in Moo som inoculated with KL102 were lower amount those with TISIR543 (p<0.05). Conclusion: KL102 was a suitable starter culture to reduce the biogenic amine formation in Moo som.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권3호
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• pp.210-218
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• 2021

Recently, pest-resistant living modified (LM) crops developed using RNA interference (RNAi) technology have been imported into South Korea. However, the potential adverse effects of unintentionally released RNAi-based LM crops on non-target species have not yet been reported. Coccinella septempunctata, which feeds on aphids, is an important natural enemy insect which can be exposed to the double-stranded RNA (dsRNA) produced by RNAi-based LM plants. To assess the risk of ingestion of Snf7 dsRNA by C. septempunctata, we first identified the species through morphological analysis of collected insects. A method for species identification at the gene level was developed using a specific C. septempunctata 12S rRNA. Furthermore, an experimental model was devised to assess the risk of Snf7 dsRNA ingestion in C. septempunctata. Snf7 dsRNA was mass-purified using an effective dsRNA synthesis method and its presence in C. septempunctata was confirmed after treatment with purified Snf7 dsRNA. Finally, the survival rate, development time, and dry weight of Snf7 dsRNA-treated C. septempunctata were compared with those of GFP and vATPase A dsRNA control treatments, and no risk was found. This study illustrates an effective Snf7 dsRNA synthesis method, as well as a high-concentration domestic insect risk assessment method which uses dsRNA to assess the risk of unintentional released of LM organisms against non-target species.

• Journal of Veterinary Science
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• 제24권6호
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• pp.82.1-82.12
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• 2023

Background: The current conventional serotyping based on antigen-antisera agglutination could not provide a better understanding of the potential pathogenicity of Salmonella enterica subsp. enterica serovar Brancaster. Surveillance data from Malaysian poultry farms indicated an increase in its presence over the years. Objective: This study aims to investigate the virulence determinants and antimicrobial resistance in S. Brancaster isolated from chickens in Malaysia. Methods: One hundred strains of archived S. Brancaster isolated from chicken cloacal swabs and raw chicken meat from 2017 to 2022 were studied. Two sets of multiplex polymerase chain reaction (PCR) were conducted to identify eight virulence genes associated with pathogenicity in Salmonella (invasion protein gene [invA], Salmonella invasion protein gene [sipB], Salmonella-induced filament gene [sifA], cytolethal-distending toxin B gene [cdtB], Salmonella iron transporter gene [sitC], Salmonella pathogenicity islands gene [spiA], Salmonella plasmid virulence gene [spvB], and inositol phosphate phosphatase gene [sopB]). Antimicrobial susceptibility assessment was conducted by disc diffusion method on nine selected antibiotics for the S. Brancaster isolates. S. Brancaster, with the phenotypic ACSSuT-resistance pattern (ampicillin, chloramphenicol, streptomycin, sulphonamides, and tetracycline), was subjected to PCR to detect the corresponding resistance gene(s). Results: Virulence genes detected in S. Brancaster in this study were invA, sitC, spiA, sipB, sopB, sifA, cdtB, and spvB. A total of 36 antibiogram patterns of S. Brancaster with a high level of multidrug resistance were observed, with ampicillin exhibiting the highest resistance. Over a third of the isolates displayed ACSSuT-resistance, and seven resistance genes (β-lactamase temoneira [bla_TEM], florfenicol/chloramphenicol resistance gene [floR], streptomycin resistance gene [strA], aminoglycoside nucleotidyltransferase gene [ant(3")-Ia], sulfonamides resistance gene [sul-1, sul-2], and tetracycline resistance gene [tetA]) were detected. Conclusion: Multidrug-resistant S. Brancaster from chickens harbored an array of virulence-associated genes similar to other clinically significant and invasive non-typhoidal Salmonella serovars, placing it as another significant foodborne zoonosis.

• Geomechanics and Engineering
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• 제35권6호
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• pp.581-591
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• 2023

North Anatolian Fault Zone is tectonically active with recent earthquakes (Mw7.6 1999-Kocaeli and Mw7.2 1999-Düzce earthquakes) and it passes through Marmara region, which is highly industrialized, densely populated and economically important part of Turkey. Many power plants, located in Marmara region, are exposed to high seismic hazard. In this study, open source OpenQuake software has been used for the probabilistic earthquake hazard analysis of Marmara region and risk assessment for the specified energy facility. The SHARE project seismic zonation model has been used in the analysis with the regional sources, NGA GMPEs and site model logic trees. The earthquake hazard results have been compared with the former and existing earthquake resistant design regulations in Turkey, TSC 2007 and TBSCD 2018. In the scope of the study, the seismic hazard assessment for a typical natural gas combined cycle power plant located in Marmara region has been achieved. The seismic risk assessment has been accomplished for a typical control building located in the power plant using obtained seismic hazard results. The structural and non-structural fragility functions and a consequence model have been used in the seismic risk assessment. Based on the seismic hazard level with a 2% probability of exceedance in 50 years, considered for especially these type of critical structures, the ratios of structural and non-structural loss to the total building cost were obtained as 8.8% and 45.7%, respectively. The results of the study enable the practical seismic risk assessment of the critical facility located on different regions.

• 한국토양비료학회지
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• 제9권3호
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• pp.183-199
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• 1976

1. The cultivated land in Korea has originally low fertility resulting in high dependence to fertilizers. 2. The total fertilizer requirement calculated by the Office of Rural Development (ORD) in 1976 was about 1,153 thousand mts, and the total amount of supply planned by the Ministry of Agriculture and Fishery was 1,010 thoushand mts which is close to the amount calculated by ORD. However, there is some gap between the amount of recommended (N, 11.6; $P_2O_5$: $K_2O$, 7.3kg/10a> and supply planned (N, 12.0; $P_2O_5$, 6.3; $K_2O$. 4.8kg/10a) fertilizers for each elements per unit area 3. For 15 years from 1960 to 1975 the fertilizer consumption of nitrogen was roughly increased from 200,000 mts to 500,000 mts; phosphorus, from 50,000 to 250,000 mts; potassium, from 10, 000 to 170,000 mts; accounting 2.5, 5, and 17 times of increase respectively. 4. The total fertilizer consumption for 5 years from 1967 to 1971 was about 100,000 mts and another 5years from 1971 to 1975 was 300,000 mts indicating three times increase. 5. The direct factors influenced to the increase of fertilizer consumption in recent years are 1) the dissemination of high yielding Tongil type rice varieties which are resistant to heavy fertilization 2) high price policy for agricaltural products 3) increased cultivation of vegetables:, fruits, and forages which require high level of fertilizers. The indirect factors are 1) dissemination of new improved agricultural techniques, 2, improvement of cultivated land conditions through irrigation system and land reform, 3) increased supply of silicate fertilizers, and 4) increase of farm income. 6. The percentage of total fertilizer consumption by rice (32%) and barley (25%) is about 57%. The ratio of total fertilizer consumption by vegetables and forages is expected to increase greatly. 7. Based on the increasing tendency of cultivated land and yield per unit area for last 10 years in each crop, total fertilizer consumptions in 1980, 1990, and 2000 year are estimated to 1,290,000, 1,580,000 and 1,870,000 mts respectively.