• Preventive Nutrition and Food Science
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• 제10권3호
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• pp.251-256
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• 2005

An extract obtained from germinated brown rice was evaluated for protective effects against the adverse effects of a chronic high fat diet. C57BL6/J mice were divided into three groups; normal diet + water group (ND), high fat diet + water group (HD), high fat diet + brown rice water extract group (HD + BE) and fed for 8 weeks (6 day/week). The ND group diet was 11 kcal fat $\%$. The HD group and HD + BE group diet were 42 kcal fat $\%$. Weight gains were not significantly different between groups. However, abdominal fat $\%$ was 1.6-fold higher in the HD group than HD + BE group. Feed consumption was significantly higher in the ND group than HD group. Serum triglyceride, total cholesterol and LDL-cholesterol increased with a high fat diet, but decreased by brown rice water extract administration to the high fat diet group. Serum HDL-cholesterol decreased by the high fat diet, but increased by the brown rice wate extract administration. Also, serum HDL-cholesterol/total cholesterol ratio was 2.3-fold higher in the HD + BE than the HD. Liver triglyceride and total cholesterol were not significantly different among groups. These data suggest that brown rice water extract administration improves the serum lipid profiles of C57BL/6J mice.

• The Korean Journal of Physiology and Pharmacology
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• 제4권2호
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• pp.91-97
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• 2000

The purpose of the present study was to determine the preventive effects of combined interventional trial of fish oil treatment and exercise training on insulin resistance of skeletal muscle in high-fat fed rats. Male Wistar rats were randomly divided into chow diet (CD), high-fat diet (HF), high-fat diet with fish oil (FO), high-fat diet with exercise training (EX), and FO+EX groups. The rats in control group were fed chow diet containing, as percents of calories, 58.9% carbohydrate, 12.4% fat, and 28.7% protein. High-fat diet provided 32% energy as lard, 18% as corn oil, 27% as carbohydrate and 23% as casein. The fish oil diet had the same composition as the high fat diet except that 100 g menhaden oil was substituted for corn oil. Insulin sensitivity was assessed by in vitro glucose transport in the soleus muscle after diet treatment and treadmill running for 4 weeks. While the FO or EX only partially prevented insulin resistance on glucose transport and visceral obesity induced by high-fat diet, these interventions completely corrected hyperinsulinemia and hyperglycemia from the high-fat diet. The rats in the FO+EX showed normalized insulin action on glucose transport, plasma chemicals and visceral fat mass. Insulin-mediated glucose transport was negatively associated with total visceral fat mass (r=－0.734; p＜0.000), plasma triglyceride (r=－0.403; p＜0.05) and lepin (r=－0.583; p＜0.001) concentrations with significance. Multiple stepwise regression analysis showed that only total visceral fat mass was independently associated with insulin-mediated glucose transport (r=－0.668; p＜0.000). In conclusion, combined interventional trial of FO+EX recovered insulin resistance on glucose transport of skeletal muscle induced by high-fat diet. Visceral fat mass might be more important factor than plasma TG and leptin to induce insulin resistance on glucose transport of skeletal muscle in high-fat fed rats.

• Nutrition Research and Practice
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• 제2권4호
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• pp.204-210
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• 2008

This study was performed to investigate effects of Chiarella vulgaris on lipid metabolism in rats fed high fat diet. Sixty 6-week-old male Wistar rats were divided into two groups; normal diet group and high fat diet group, then the rats in each group were further divided into three subgroups and fed 0%, 5% and 10% (w/w) chlorella-containing diets, respectively, and raised for 9 weeks, Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and total protein and albumin concentration were not different among groups. Serum total lipids and liver TG concentration were significantly lower in 5% and 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Serum TG, serum total cholesterol, liver total lipid and liver total cholesterol concentrations were significantly lower in 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Fecal total lipid, TG and total cholesterol excretions were significantly higher in 5% and 10% chlorella groups than 0% chlorella groups in normal diet and high fat diet groups, respectively (p<0.05). These results suggest that Chlorella vulgaris is effective for prevention of dyslipidemia which may be due to the modulation of lipid metabolism and increased fecal excretion of lipid.

• 한국농촌생활과학회지
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• 제10권2호
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• pp.71-77
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• 1999

This study examined the effect of the pine needle sap administration on serum lipid composition and liver antioxidant defense system in rats fed with high fat diet. Forty eight male Sprague-Dawley rats were divided into six groups. three control groups were fed with 5% lipid diet and three high fat groups were fed with 30% lipid diet. Each group was administered with the following pine needle sap respectively : C-0.0, 1.ml water, C-0.5, 0.ml pine needle sap and 0.ml water C-1.0, 1.ml pine needle sap : H-0.0, 1.ml water, H-0.5, 0.ml pine needle sap and 0.ml water H-1.0, 1.0ml pinus needle sap. After 4 weeks of experimental periods the level of serum obtained and serum lipid was measured respectively. The results were as follows 1. Significant low level was observed for food intake, weight gain, FER in the experimental group administered with the pine needle sap. 2. No difference of the concentration of serum triglyceride was observed out the experimental group with 5% fat diet, however out of the experimental group with 30% fat diet it was low in (H-1.0) group where 1.0ml of the pine needle sap was administrated. 3. In the experimental group with 5% fat diet the concentration of serum total cholesterol and LDL cholesterol was low, especially the hypocholesteromic effect in serum administered by the pine needle sap was significantly high in group C-1.0 where 1.0ml of pine needle sap was administered. In the high fat group provided with 30% fat when the pine needle sap was administered there was a tendency that concentration of total cholesterol and LDL cholesterol decreased however there was no difference significantly. 4. Serum GOT activity was relatively high in high fat diet group. However, when 1$m\ell$ of pine needle sap was administered the activity was lower than that of the control group in H-1.0 group and the activity level was similar with that of the high fat diet group in c-1.0 group. There was no difference of serum GPT activitives followed by the administered of pine needle sap among the experimental group. 5. Liver TBARS levels were high in general in high fat diet group, however it showed no difference when the pine needle sap with different concentration level was administered. The results of this study indicate that the pine needle sap administration was effective in decreasing the food intake and weight gain of the experimental animals in the high fat diet and also effective in decreasing the levels of serum triglyceride, total cholesterol and LDL cholesterol and GOT activities. However it was not effective to change the level of TBARS and GSH -Px activities of liver. Thus, it was found that the pine needle sap administration was effective for the improvement of serum lipid composition condition of the experimental animals in the high fat diet group but it was not effective in the antioxidant defense system of liver.

• Journal of Applied Biological Chemistry
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• 제54권3호
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• pp.197-205
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• 2011

This study was carried out to investigate the dietary effects of Monascus pilosus mycelial extract on obesity in high-fat with cholesterol-induced obese rat models. It was observed that M. pilosus mycelial extract contains $25.85{\pm}1.98mg%$ of total monacolin K without citrinin by highperformance liquid chromatography (HPLC). The rats were randomly divided into 2 groups; normal control and a high-fat with cholesterol diet group. The high-fat with cholesterol diet group was fed a 5L79 diet with an added 15% lard and 1% cholesterol supplemented diet for 3 weeks for induction of obesity. After induction, obesity was confirmed by checking obesity indexes, the animals were divided into 4 groups (n=5); first, the normal control (NC), and then taken from the obese model of rats, a high-fat with cholesterol diet obesity control group (HF), 0.5% M. pilosus mycelial extract supplemented high-fat with cholesterol diet group (MPMs), 2% conjugated linoleic acid supplemented high-fat with cholesterol diet group (CLA) for 7 weeks. Body weight gains, obesity indexes, and body fat contents in the experimental groups (MPMs and CLA) were decreased compared with HF group. Feed Efficiency Ratio (FER) in MPMs was significantly lower than that of HF without change of feed intake. These results suggested that the anti-obesity effects of the M. pilosus mycelial extracts (MPMs) could prevent obesity induced by high-fat with cholesterol diet possibly via inhibition of lipid absorption.

• 대한본초학회지
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• 제39권4호
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• pp.1-9
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• 2024

Objective : In this study, the anti-obesity effect of Fragaria orientalis (FO) on high-fat diet-induced obese mice was investigated. Drug treatment methods are widely used as obesity treatment methods, but research using various natural products is being conducted due to safety concerns. This study aims to evaluate the anti-obesity effect of FO extract, a natural product derived from Mongolia. Methods : C57BL/6 mice were used and divided into three groups, normal diet group, high-fat diet group, and high-fat diet with FO oral treatment group at a dose of 300 mg/kg. Extract was orally provided everyday for 6 weeks. Body Weight and food intake were measured every 2 days and blood lipid profiles and liver function in the sacrificed mice were evaluated. In addition, protein expression in hepatic tissue and histomorphological changes in liver and adipose tissue were observed. Results : Body weight, adipose tissue weight and FER were significantly lower in a high-fat diet with FO treatment than fed only high-fat diet. There was a significant difference between the high-fat diet and the FO-treated high-fat diet mice. As a result of analyzing lipid metabolism-related genes in hepatic tissue, all of p-AMPK, p-ACC, PPAR-α, CPT-1, and UCP-1 showed significant increases, and PPAR-γ also decreased significantly compared to the high-fat diet group. Conclusion : Overall, these results indicate that FO is effectual in improving obesity, suggesting that it can be used as a possible material for anti-obesity agents or functional supplements for weight control.

• 한국작물학회지
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• 제58권4호
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• pp.443-450
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• 2013

Effects of newly developed functional rice grains Goami (high-amylose rice) and Nokwon (green-kerneled rice) on the glucose metabolism and antioxidative defense system in C57BL/6N mice under high fat diet condition were investigated. Animals were randomly divided into five groups (n = 8) and given experimental diets for eight weeks: normal control diet (NC), high fat diet (HF), and high fat diet supplemented with white rice (HF-WR), Goami rice (HF-GR), and Nokwon rice (HF-NR). At the end of the experimental period, the HF group exhibited markedly higher blood glucose level, insulin concentration, plasma lipid peroxidation and lower hepatic glycogen concentration than that exhibited by NC group. However, diet supplementation of Goami and Nokwon suppressed the high fat diet-induced hyperglycemia and oxidative stress through inhibition of the glucose-regulating enzymes and enhancement of the antioxidant enzymes activities. The results illustrate that the new functional rice Goami and Nokwon may be useful in the development of functional foods with preventive effect against high fat diet-induced hyperglycemia and oxidative stress.

• 한국체육학회지인문사회과학편
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• 제51권3호
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• pp.353-362
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• 2012

본 연구는 4주령의 male Wister rat을 대상으로 4주간 등열량 고지방식이 (50% calories from fat) 또는 지구성 운동 (treadmill running, slop 8%, 23m/min, 120분/일, 5일/주)이 미토콘드리아 생합성과 인슐린 저항성에 미치는 영향을 알아보는 것이다. 4주간의 등열량 고지방식이는 일반 사료 집단에 비해 체중과 체지방량에 차이가 없었고, 혈중 포도당, 인슐린 수준 및 골격근내의 인슐린 유발 포도당 이동률도 4주간 등열량 고지방식이 및 일반 사료 집단 간에 차이가 없는 것으로 나타났다. 4주간 등열량 고지방식이와 지구성 운동을 병해 실시한 집단의 체중, 체지방, 혈중 포도당 수준도 다른 3집단과 차이가 없는 것으로 나타났다. 그러나 지구성 운동을 실시한 집단은 고지방식이 집단보다 혈중 인슐린 수준이 유의하게(p<0.05) 낮게 나타났다. 4주간의 등열량 고지방식 또는 지구성 운동은 미토콘드리아 생합성을 증가시키지 않았으나 복합처치의 경우 유의하게(p<0.05) 증가시켰고, 이러한 현상은 혈중 유리지방산의 증가와 함께 나타났다(p<0.05). 이상의 연구 결과들을 종합하면 4주간의 등열량 고지방식과 지구성 운동 복합처치는 인슐린 저항성을 발생시키지 않고 미토콘드리아 표지인자를 유의하게 증가시킴으로 미토콘드리아 생합성을 안정적으로 증가시키는데 좋은 대안이 될 것으로 생각된다.

• 동아시아식생활학회지
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• 제7권4호
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• pp.468-474
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• 1997

To evaluate the effect of feeding time and high fat diet on weight gain and blood components in rats, the classification of groups were night(P.M. 8:00-next day A.M. 8:00), day(A.M. 8:00 P.M. 8:00) and ad-libitum(24 hours)group as feeding time. Each group has also 2 groups by fat level(high-fat diet, standard diet). Therefore, the 36 experimental animals were divided into 6 groups. The results of this study can be summarized as follows. Food intake of SA(standard diet / ad-libitum) and SN(standard diet/night) groups was significantly higher than the others, and HA(high fat diet /ad-libitum) and HD(high fat diet /day) groups was significantly lower than the others. Body weight gains of HN group and SA group were significantly higher than the others in 5 weeks. The blood contents of total cholesterol, triglyceride, protein and albumin were no significant difference among experimental groups, but the blood contents of glucose were higher in HN and SD groups than SN and HA groups. Accordingly, eating before asleep and high fat diet bring about one's overweight and abnormal blood concentration.

• Journal of Nutrition and Health
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• 제37권4호
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• pp.266-272
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• 2004

This study investigated the effects of soyoligosaccharide consumption on lipid profile of plasma, liver and feces and immune responses in Sprague-Dawley male rats. Sixty male Sprague-Dawley rats 4-wk-old were provided the soyo-ligosaccharide containing diets for 4 weeks (0, 100 g/kg diet); each of these diets was supplemented with either 70 or 200 g fat/kg diet, giving a total of 4 experimental groups. The effect of weight reduction was most significantly observed in the group fed low fat and soyoligosacchairde diet. The plasma total lipid and cholesterol contents were not changed by either fat proportion or soyoligosaccharide supplementation in the diets. Also the plasma triglyceride lowering effect by soyoligosaccharide was not observed in rats fed either low fat or high fat diet. However, the significant decrease in TG contents was found with rats fed high fat diets compared to the control/no soyoligosaccharide diet. Elevation of plasma LDL-cholesterol and reduction of HDL-cholesterol by feeding high fat diet was not altered by supplementing soyoligosaccharide. This was also applied to the liver lipid profiles. The significant increases in liver total lipid, trigly-cerides and cholesterol by high fat diet was not abolished by feeding soyoligosaccharide. However, the desirable effects of feeding soyoligosaccharide were found with total lipid and cholesterol excretion through feces in rats fed high fat diets. Immune organ weights and spleen cell proliferations did not affected by experimental diets. These results de-monstrated that soyoligosaccharide intakes increased the lipid output via feces, especially in rats fed the high fat diet, but more researches are needed on immune responses.