• 제목/요약/키워드: high velocity cell

검색결과 132건 처리시간 0.03초

압밀, 토모그래피, 액상화시험에서 벤더엘리먼트의 적용 (Application of Bender Elements in Consolidation, Tomography, and Liquefaction Tests)

  • 이종섭;이창호
    • 한국지반공학회논문집
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    • 제22권8호
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    • pp.43-54
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    • 2006
  • 본 논문은 벤더 엘리먼트를 압밀, 토모그래피, 그리고 액상화에 적용하는 방법을 다루었다. 모래와 점토를 이용하여 압밀 시험을 수행하면서 전단파 속도를 측정하여 하중 재하 및 제하 시기를 평가하였다. 전단파 속도는 유효응력의 함수이므로 전단파 속도를 이용하여 하중 재하시기를 결정할 수 있으나, 침하량 기준과 전단파 속도(유효응력) 메카니즘이 다를 수 있으므로 주의해야 한다. 또한 전단파 속도는 고결화 제어 구간(cemented controlled regime)과 응력 제어 구간(stress controlled regime)으로 구별할 수 있어 응력이력현상을 확인할 수 있다. 벤더 엘리먼트가 설치된 고정된 프레임에서 전단파 토모그래피가 가능하다. 낮은 구속응력상태와 진 삼축 장비 내에서 토모그래피 실험을 수행한 결과 전단파 토모그래피는 평균유효응력과 관련된 전단파 속도 변화의 관측에 사용될 수 있음을 보여 주였다. 한편 액상화와 같은 어떤 현상을 관측하기 위하여 반복 주기가 높은 방식의 전단파 트랜스-일루미네이션(trans-illumination)이 적용되었다. 전단파 속도와 크기의 전개는 액상화 동안 과잉간극수압의 경향과 평행함을 알 수 있었다. 본 논문에서 소개한 적용들은 벤더 엘리먼트가 실내 시험에서 전단파 검측에 상당히 효과적인 방법이 될 수 있음을 보여준다.

Hollandite $Nax(Ti_8-_xCr_x)O_{16}$의 이온 전송과 고유전율을 위한 분자 시뮬레이션 (Molecular Simulation for Ion Transport and High Frequency Dielectric of the Hollandite $Nax(Ti_8-_xCr_x)O_{16}$)

  • 정인호;최용성;이경섭
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2008년도 제39회 하계학술대회
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    • pp.2299-2300
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    • 2008
  • The Velocity Autocorrelation Function (VAF) of the sodium ions is calculated for a range of temperature from 250K to 1000K and converted into the linear ac-conductivity and ac-susceptibility response via Fourier transformation. A peak is found in the conductivity around $6{\times}10^{12}$ Hz that has some of the character of a Poley absorption. Here it is shown to be due to an harmonically coupled site vibrations of the sodium atoms, which extend only over a limited range. At frequencies below the peak the conductivity tends towards a constant i.e. dc value corresponding to a constant flow of ions through the simulation cell. At high temperatures the conductivity due to this ion transport process behaves like a metal with an insulator to metal transition occurring around a specific temperature.

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이온 전송 및 고주파 유전 흡수 (Ion Transport and High Frequency Dielectric Absorption)

  • 윤주호;최용성;황종선;이경섭
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2008년도 하계학술대회 논문집 Vol.9
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    • pp.379-380
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    • 2008
  • Molecular Dynamics (MD) simulations have been used to model the dynamics of the charge-compensating sodium ions in the non-stoichiometric hollandite Nax$(Ti_{8-x}Cr_x)O_{16}$. These interstitial ions reside in 'tunnels' in the crystal structure and move under the forces of both the ions making up the cage structure and the many body interactions of the other sodium ions in the tunnel. The Velocity Autocorrelation Function (VAF) of the sodium ions is calculated for a range of temperature from 250K to 1000K and converted into the linear ac-conductivity and ac-susceptibility response via Fourier transformation. A peak is found in the conductivity around $6\times10^{12}$ Hz that has some of the character of a Poley absorption. Here it is shown to be due to an harmonically coupled site vibrations of the sodium atoms, which extend only over a limited range. At frequencies below the peak the conductivity tends towards a constant i.e. dc value corresponding to a constant flow of ions through the simulation cell. At high temperatures the conductivity due to this ion transport process behaves like a metal with an insulator to metal transition occurring around a specific temperature.

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고양이의 내측 및 외측 척수망상로 세포의 전기생리학적 비교연구 (A Comparative Study on the Electrophysiological Properties of Medial and Lateral Spinoreticular Tract Cells in Cats)

  • 이석호;전제열;박춘옥;구용숙;김전;성호경
    • The Korean Journal of Physiology
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    • 제24권1호
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    • pp.181-194
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    • 1990
  • Vasomotor area로 알려져 있던 외측연수망상체는 최근 rostral ventrolateral medulla로 불리 면서 sympathoexcitatory neuron이 집중적으로 분포하는 연수내 부위로서, 체성교감신경반사에 중요한 역할을 하는 것으로 알려지고 있어 이 부위로의 입력정보가 그동안 많은 연구가 되어온 내측 척수망상로 세포와 어떤 차이를 보이는가를 규명하고자 하였다. 1) Medial SRT 세포는 34 cell중 약 60%가 동측으로 향하였으나, lateral SRT cell의 경우 47 cell의 약 60%가 반대측으로 향하였다. 2) 각 군의 세포를 말초자극에 대한 반응성에 따라, LT cell, Deep cell, HT cell및 WDR cell로 나누었으며 유해자극을 전달하는 세포를 HT와 WDR cell이라 하고, 무해자극을 전달하는 세포와 유해자극을 전달하는 세포의 비율로 볼 때, 다른 부위에 비하여 rostral VLM에 유해자극정보가 비교적 많이 전달됨을 볼 수 있었다. 3) 평균 전도 속도는 각 군간에 유의한 차이가 없었으나, Deep cell은 HT cell보다 유의하게 빠른 전도속도를 보였다. 4) Medial SRT 세포는 척수 회백질 내에서 Rexed laminae VII및 VIII에 주로 분포하며, LRN projecting SRT cell의 경우엔 전 lamina에 걸쳐 고루 분포하였다. 이상의 결과는 내측 및 외측 망상체간의 말초 입력 정보의 차이를 보여주고 있으며, 연수 망상체의 세 부위중 특히 rostral ventrolateral medulla로 많은 동통정보가 입력되고 있어 RVLM의 기능중 하나인 체성교감반사의 통합과 연관된 역할을 하리라 기대된다.

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Substitution of Glycine 275 by Glutamate (G275E) in Lipase of Bacillus stearothermophilus Affects Its Catalytic Activity and Enantio- and Chain Length Specificity

  • Kim, Myung-Hee;Kim, Hyung-Kwoun;Oh, Byung-Chul;Oh, Tae-Kwang
    • Journal of Microbiology and Biotechnology
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    • 제10권6호
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    • pp.764-769
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    • 2000
  • The lipase gene(lip) from Bacillus stearothermophilus was recombined in vitro by utilizing the DNA shuffling technique. After four rounds of shuffling, transformation, and screening based on the initial rate of clear zone formation on a tricaprylin plate, a clone (M10) was isolated, the cell extract of which showed about 2.8-fold increased lipase activity. The DNA sequence of the mutant lipase gene (m10) showed 3 base changes, resulting in two cryptic mutations and one amino acid substitution: S113($AGC{\rightarrow}AGT$), L252 ($TTG{\rightarrow}TTA$), and G275E ($GGA{\rightarrow}GAA$). SDS-PAGE analysis revealed that the increased enzyme activity observed in M10 was partly caused by high expression of the m10 lipase gene. The amount of the expressed G275E lipase was estimated to comprise as much as 41% of the total soluble proteins of the cell. The maximum velocity ($V_{max}$) of the purified mutant enzyme for the hydrolysis of olive oil was measured to be 3,200 U/mg, which was 10% higher than that of the parental (WT) lipase (2,900 U/mg). Its optimum temperature for the hydrolysis of olive oil was $68^{\circ}C$ and it showed a typical $Ca^{2+}$-dependent thermostability, properties fo which were the same as those of the WT lipase. However, the mutant enzyme exhibited a high enantiospecificity towards (S)-naproxen compared with the WT lipase. In addition, it showed increased hydrolytic activity towards triolein, tricaprin, tricaprylin, and tricaproin.

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Heterologous Expression and Characterization of a Thermostable Exo-β-D-Glucosaminidase from Aspergillus oryzae

  • Wu, Dingxin;Wang, Linchun;Li, Yuwei;Zhao, Shumiao;Peng, Nan;Liang, Yunxiang
    • Journal of Microbiology and Biotechnology
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    • 제26권2호
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    • pp.347-355
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    • 2016
  • An exo-β-D-glucosaminidase (AorCsxA) from Aspergillus oryzae FL402 was heterologously expressed and purified. The deduced amino acid sequence indicated that AorCsxA belonged to glycoside hydrolase family 2. AorCsxA digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-D-glucosaminidase (CsxA) activity. AorCsxA exhibited optimal activity at pH 5.5 and 50℃; however, the enzyme expressed in Pichia pastoris (PpAorCsxA) showed much stronger thermostability at 50℃ than that expressed in Escherichia coli (EcAorCsxA), which may be related to glycosylation. AorCsxA activity was inhibited by EDTA and most of the tested metal ions. A single amino acid mutation (F769W) in AorCsxA significantly enhanced the specific activity and hydrolysis velocity as revealed by comparison of Vmax and kcat values with those of the wild-type enzyme. The three-dimensional structure suggested the tightened pocket at the active site of F769W enabled efficient substrate binding. The AorCsxA gene was heterologously expressed in P. pastoris, and one transformant was found to produce 222 U/ml activity during the high-cell-density fermentation. This AorCsxA-overexpressing P. pastoris strain is feasible for large-scale production of AorCsxA.

Spatio-Temporal Variations of Harmful Algal Blooms in the South Sea of Korea

  • Kim, Dae-Hyun;Denny, Widhiyanuriyawan;Min, Seung-Hwan;Lee, Dong-In;Yoon, Hong-Joo
    • 대한원격탐사학회지
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    • 제25권6호
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    • pp.475-486
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    • 2009
  • Harmful algal blooms (HAB) caused by the dominant species Cochlodinium polykrikoides (C. polykrikoides) appear in the South Sea of Korea and are particularly present in summer and fall seasons. Environmental factors such as water temperature, weather conditions (air temperature, cloud cover, sunshine, precipitation and wind) influence on the initiation and subsequent development of HAB. The purpose of this research was to study spatial and temporal variations of HAB in the Yeosu area using environmental (oceanic and meteorological) and satellite data. Chlorophyll-a concentrations were calculated using Sea-viewing Wide Field-of-view Sensor (SeaWiFS) images by an Ocean Chlorophyll 4 (OC4) algorithm, and HAB were estimated using the Red tide index Chlorophyll Algorithm (RCA). We also used the surface velocity of sequential satellite images applying the Maximum Cross Correlation method to detect chlorophyll-a movement. The results showed that the water temperature during HAB occurrences in August 2002-2008 was $19.4-30.2^{\circ}C$. In terms of the frequency of the mean of cell density of C. polykrikoides, the cell density of the HAB found at low (<300 cells/ml), medium (300-1000 cells/ml), and high (>1000 cells/ml) levels were 27.01%, 37.44%, and 35.55%, respectively. Meteorological data for 2002-2008 showed that the mean air temperature, precipitation, wind speed and direction, and sunshine duration were $22.39^{\circ}C$, 6.54 mm/day, 3.98 m/s (southwesterly), and 1-11.7 h, respectively. Our results suggest that HAB events in the Yeosu area can be triggered and extended by heavy precipitation and massive movement of HAB from the East China Sea. Satellite images data from July to October 2002-2006 showed that the OC4 algorithm generally estimated high chlorophyll-a concentration ($2-20\;mg/m^3$) throughout the coastal area, whereas the RCA estimated concentrations at $2-10\;mg/m^3$. The surface velocity of chlorophyll-a movement from sequential satellite images revealed the same patterns in the direction of the Tsushima Warm Current.

고효율 실리콘 태양전지(II)-확산형 실리콘 태양전지에 대한 모의 실험 (High Efficiency Silicon Solar Cell(II)-Computer Modeling on Diffused Silicon Solar Cell)

  • 강진영;이종덕
    • 대한전자공학회논문지
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    • 제18권4호
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    • pp.49-61
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    • 1981
  • N+P, N+PP+ 형 태양전지를 제조하여 얻은 실험자료들을 근거로 실리콘 접합형 태양전지에 일반적으로 적용할 수 있는 컴퓨터 모의 실험 프로그램을 개발하고, 이의 유용성을 확인하였다. 이 모의 실험 프로그램은 N+P, P+N, N+PP+, P+NN+형의 실리콘 태양전지에 적용할 수 있는 것으로, 입사광은 AMI, 등강도 광원, 인공조명으로 많이 사용되는 GE -ELH 광원이고, AR코팅은 Si3N4형과 투과도가 파장에 관계없는 일정형 2종류가 있으며, 프로그램에서 이들 전지의 구조, 광원, AR 코팅 종류에 대한 파장 특성분포도 쉽게 변화시킬 수 있도록 되어 있다. 이 모의실험의 결과들을 토대로 N+와 P 영역에서의 평균도오핑농도와 전지의 두께, AMI 스펙트럼에 대한 집광도, 앞면 접합깊이에 대하여 효율이 최대가 되는 최적치들을 구하였으며, 앞면의 표면 재결합 속도, 접합부에서의 캐리어의 유효 수명, 누설저항에 대하여는 허용 한계치로, 기타 효율변화인자로서 동작온도 직렬저항과 전기장의 세기에 대하여는 효율의 변화율로서 파라미터들이 효율에 미치는 영향들을 분석하였다.

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사행수로의 흐름구조 및 난류특성 (Flow Structure and Turbulence Characteristics in Meandering Channel)

  • 서일원;이규환;백경오
    • 대한토목학회논문집
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    • 제26권5B호
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    • pp.469-479
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    • 2006
  • 본 연구에서는 사행수로에서 주 흐름과 이차류의 특성을 정량적으로 분석하고자 중심각이 $150^{\circ}$이고 사행도 1.52인 S자형 사행수로에서 실험을 수행하였다. 평균수심과 유량을 달리하여 다양한 실험 조건 하에서 수행한 실험을 통해 다중 만곡부를 갖는 사행수로에서 이차류의 공간적 변화양상을 관찰하였다. 실험결과, 주 흐름은 실험조건에 관계없이 직선구간에서 좌우 대칭적인 유속분포를 보였고, 만곡부에서는 내안쪽에 최대유속이 발생하고 외안쪽에 최소유속이 발생하는 현상을 발견 할 수 있었다. 이렇게 주 흐름이 최단노선을 따라 발생하는 현상은 기존 연구자들의 결과와 일치하는 것이다. 이차류의 거동은 첫번째 만곡부보다 두 번째 만곡부에서 더욱 활발하게 발달하고 외안 회전류가 뚜렷히 나타남을 발견하였다. 이차류의 강도는 직선구간에서는 낮게 나타나고 만곡부에서는 증가하는 주기적인 현상을 보였으며, 만곡부의 이차류 강도가 직선구간보다 2~3배 크게 나타났다. 또한, 두 번째 만곡부에서 이차류 강도의 최대값이 발생했다. 주 흐름방향의 난류 강도와 Reynolds 전단응력을 분석한 결과, 주 흐름 난류 강도는 주 흐름의 유속편차가 클수록 증가하는 것으로 나타났고 Reynolds 전단응력은 주 흐름방향 유속의 편차가 크게 벌어지는 동시에 이차류가 활발히 생성되는 지점에서 크게 나타나는 경향을 보였다.

The Electrophysiological Characteristics of Medullospinal Tract Cells in Cat Ventrolateral Medulla

  • Lee, Woo-Yong;Kim, Sang-Jung;Kim, Jun
    • The Korean Journal of Physiology
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    • 제25권2호
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    • pp.211-221
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    • 1991
  • Although the existence of nerve cells which determine the activity of sympathetic nervous system in ventrolateral medulla is advocated recently, there are wide varieties on the location and function of them according to authors. Present study aimed to identify and characterize the medullospinal tract cells in rostral and caudal medulla of cats .which branch to the lateral horn of the upper thoracic spinal cord. Cats were anesthetized with ${\alpha}-chloralose$. The upper thoracic spinal cord and floor of the IVth ventricle were exposed. Medullospinal tract cells in rostral and caudal medulla were identified by anti-dromic stimulation of the intermediolateral nucleus in the upper thoracic cord and then the location and physiological characteristics of these cells were studied. A total of seventy cells in medulla had constant latency and responded to high frequency stimulation to thoracic cord. Among them fifty-six cells were identified as medullospinal tract cells either by collision with spontaneous activities or activities evoked by sciatic nerve stimulation(27/56), or by determining the refractory period (29/56). Thirty-one of these cells branched to the contralateral thoracic spinal cord, twenty-one cells to the ipsilateral side and remaining four cells branched to both sides. The conduction velocity of cells branching to the contralateral side was $29{\pm}2.9\;m/sec$ and that of cells to the ipsilateral side was $39.1{\pm}6.0\;m/sec$. When medulla was devided into two by a horizontal plane at 3 mm rostral to the obex, fifty-one among seventy cells were in the rostral medulla and nineteen were in the caudal medulla. The conduction velocities of these two groups were $21.6{\pm}1.0\;and\;33.3{\pm}3.9\;m/sec$, respectively. In this study, we confirmed the existence of two groups of medullospinal tract cells in rostral and caudal ventrolateral medulla, which branch to the lateral horn of thoracic cord and these cells have relatively few spontaneous activities and rapid conduction velocity, so we concluded that these cells are different from the previously known sympatho-related cells in ventrolateral medulla.

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