• Water for future
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• v.17 no.2
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• pp.85-97
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• 1984

Measuring the river flow and water quality in the midstream of the Nakdong River, the natural purification status in examined through the analyses of the elements which affect the variation of dissolved oxygen, and DO model is evaluated to the midstream reach of the river. The major results of this study are as follows; the pruification factor of the of the river is relatively high, it is worried over eutrophication considering much production of algae, and it is evaluated that important factor affecting the DO value computed by the proposed DO model are in order of reaeration coefficient, carbonaceous BOD and deoxygenation constant.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.719-722
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• 2001

Silk proteins from Nephila clavipes are fibrous proteins containing repetitive sequences with both crystalline and amorphous domains. In order to obtain high-level production of silk protein, the synthetic genes had 16 contiguous units of the consensus repeat sequence of the silk protein were expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. For production of recombinant silk protein in large amounts, pH-stat fed-batch cultures were carried out. The recombinant silk protein was produced as soluble forms in E. coli, and the recombinant silk protein content was as high as 11% of the total protein. When cells were induced at $OD_{600}$ of 60, the amount of silk protein produced was 6.49 g/L. After simple purification steps, 9.2 mg of silk protein that was more than 80% pure was obtained from a 50 mL culture, and the recovery yield was 26.3%.

• The Korean Journal of Food And Nutrition
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• v.9 no.3
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• pp.242-246
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• 1996

High viscous hyaluronic acid complex from Klebsiella sp. L-10 NTG 50 mutant was purified by two-phase extraction system using PEG-K2HP04 and its physicochemical properties were Investigated. Viscosity of the purified hyaluronic acid complex was decreased as temperature and salts concentration were Increased and also showed low viscosity at below pH 5.0 and above pH 11.0. Hardness, cohesiveness and adhesiveness of the purified hyaluronic acid complex were 1, 20kg, 1.91 and 0.62, respectively. Water holding capacity was 6.9ml per gram of the purified hyaluronic acid complex powder.

• Fisheries and Aquatic Sciences
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• v.15 no.3
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• pp.191-195
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• 2012

We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powdered P. siliquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative light-scattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol (3:2, v/v), to obtain 10.96 mg fucosterol with 96.8% purity from 50 mg of crude extract; the recovery rate was approximately 90.5%.

• Korean Journal of Environmental Agriculture
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• v.26 no.1
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• pp.25-35
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• 2007

Excess runoff contaminated with N and P can impact the quality of downstream water. It has been known that aquatic plants improve the water quality through their intake of organic or inorganic nutrients. This study was conducted to select aquatic plants having high purification ability for nutrient N and P, and mineral nutrients related to EC such as K, Ca, Na, Cl, and $SO_4$ in raw sewage water in greenhouse. We assessed nutrient phytoremediation potential of alien hydrophyte and hydro-crop as well as native landscape hydrophyte to select suitable aquatic plant applied to artificial wetland and buffering site of stream-side. The amount of irrigation water during whole growing period of aquatic plane ranged from 225 L $m^{-2}$ to 444 L $m^{-2}$. Oryza sativa, Typha orientalis, Zizania latifolia, Aster subulatus, Coix lachryma-jobi var. mayuen, Paspalum disdichum var. indutum which had high biomass consumed the large amount of irrigation water over 350 L $m^{-2}$. As a result of analysis of water purification effect N and P content of shoot biomass, and media soil after experiment, Oryza sativa, Zizania latifolia, Aster subulatus, Coix lachryma-jobi var. mayuen, Paspalum distichum var. indutum showed high purification ability about eutrophication elements such as T-N and T-P. It is presented that Pistia stratiotes, Eichhornia crassipes, and Paspalum distichum var. indutum had excellent purification ability about K, Ca, Na, and Cl. Moreover, Paspalum distichum var. indutum greatly removed $SO_4$ in row sewage water.

• BMB Reports
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• v.44 no.4
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• pp.279-284
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• 2011

The glutathione S-transferase (GST) system is useful for increasing protein solubility and purifying soluble GST fusion proteins. However, purifying half of the GST fusion proteins is still difficult, because they are virtually insoluble under non-denaturing conditions. To optimize a simple and rapid purification condition for GST-pyruvate kinase muscle 2 (GST-PKM2) protein, we used 1% sarkosyl for lysis and a 1 : 200 ratio of sarkosyl to Triton X-100 (S-T) for purification. We purified the GST-PKM2 protein with a high yield, approximately 5 mg/L culture, which was 33 times higher than that prepared using a conventional method. Notably, the GST-high-temperature requirement A2 (GST-HtrA2) protein, used as a model protein for functional activity, fully maintained its proteolytic activity, even when purified under our S-T condition. This method may be useful to apply to other biologically important proteins that become highly insoluble in the prokaryotic expression system.

• Archives of Pharmacal Research
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• v.21 no.3
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• pp.291-297
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• 1998

Cop protein has been overexpressed in Escherichia coli using a T7 RNA polymerase system. Purification to apparent homogeneity was achieved by the sequential chromatography on ion exchange, affinity chromatography, and reverse phase high performance liquid chromatography system. The molecular weight of the purified Cop was estimated as 6.1 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). But the molecular mass of the native state Cop was shown to be 19 kDa by an analytical high performance size exclusion chromatography, suggesting a trimer-like structure in 50 mM Tris-HCI buffer (pH 7.5) containing 100 mM NaCl. Cop protein Was calculated to contain $39.1% {\alpha}-helix, 16.8% {\beta}-sheet$, 17.4% turn, and 26.8% random structure. The DNA binding property of Cop protein expressed in E. coli Was preserved during the expression and purification process. The isoelectric point of Cop was determined to be 9.0. The results of amino acid composition analysis and N-terminal amino acid sequencing of Cop showed that it has the same amino acid composition and N-terminal amino acid sequence as those deduced from its DNA sequence analysis, except for the partial removal of N-terminal methionine residue by methionyl-aminopeptidase in E. coli.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.29 no.6
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• pp.276-282
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• 2019

The purpose of this study was to analyze the structure of general filter using ultrafine filter (Profilter), dust collector filter, HEPA (HAPA-High Efficiency Particulate Air) filter, deodorized filters, etc. of air purifiers and to study new types of purified filters that can improve ultrafine dust, harmful gases, and sterilization cleanup performance. The study was also conducted by adding photocatalyst filters to the existing step-by-step filtration filter types, which were proposed in the design three coupling structure filters of the left and right UV-LED installation frames and the photocatalyst coating honeycomb frame. Future research is needed on the effect of photocatalyst filters. This study was to investigate the application and structure of photocatalyst filters to air purifiers.

• Journal of the Korean Applied Science and Technology
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• v.24 no.4
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• pp.383-392
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• 2007

This paper is studied on the efficient purification process of dimethyl carbonate (DMC) from the melt layer crystallization combining crystallization process, sweating process and distillation recovery process. Purity and yield of DMC crystal depended mainly on the crystallization temperature, cooling rate, sweating termperature sweating rate. Through the optimization of crystallization and sweating operation, DMC crystal can be upgraded to very high purity over 99.9% and high yield over 85%.

• Applied Chemistry for Engineering
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• v.25 no.3
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• pp.330-336
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• 2014

Purification of indole contained in model coal tar absorption oil was examined by extraction-distillation-crystallization combination. The absorption oil consists of nine components such as four kinds of nitrogen heterocyclic compounds (9.2% quinoline, 2.4% iso-quinoline, 4.7% indole, 2.4% quinaldine), three kinds of bicyclic aromatic compounds (14.2% 1-methylnaphthalene, 31.8% 2-methylnaphthalene, 23.5% dimethylnaphthalene), 5.5% biphenyl and 3.3% phenyl ether. 99.5% indole was recovered by combination of formamide extraction-distillation-solute crystallization using n-hexane. Furthermore, the recovery process of indole contained in coal tar absorption oil was studied by using the experimental results obtained by each operation of this work.