• 한국식품영양학회지
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• 제24권2호
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• pp.239-245
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• 2011

당의 종류와 혼합비가 쿠키의 품질에 미치는 영향을 살펴보았다. 당 함량이 30%로 고정된 배합비에서 당 종류를 과당, 고과당, 전화당으로 달리하여 첨가하였을 때 고과당은 적정한 퍼짐성을 가졌으며, 관능평가에서도 전체적으로 좋은 평점을 나타내었다. 가장 적절한 물성과 관능을 나타낸 고과당을 함량별로 첨가하여 함량에 따른 영향을 살펴보았을 때, 고과당의 함량이 증가할수록 수분 보유 능력과 퍼짐성은 증가하고, 관능적 차이는 나타나지 않았으나 색상은 어두워졌다. 또한 고과당 함량이 40%인 경우에는 다른 함량비와 상대적인 유의차가 나타났다. 단일당만의 효과와는 달리, 고과당에 전화당을 혼합한 경우에는 전화당의 함량이 증가할수록 퍼짐성은 감소하고 경도는 증가하는 것으로 나타났다. 전화당에 대한 고과당의 혼합비는 고과당을 50%까지 혼합해도 기호도의 차이는 없는 것으로 나타났으며, 전화당:고과당의 비율이 75:25인 경우가 가장 기호도가 높은 것으로 나타났다. 전체적으로 당의 종류가 쿠키의 품질에 미치는 영향은 각각 상대적으로 나타났으며, 혼합한 경우 당 종류에 따라 쿠키의 물성과 관능적 특성이 변화되는 것으로 나타났다.

• 한국미생물·생명공학회지
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• 제20권4호
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• pp.437-444
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• 1992

분쇄마찰매체함유 불균일상 효소반응계를 활용한 생전분의 무증자당화법은 고농도, 고순도의 포도당을 고효율로 직접 얻을 수 있는 새로운 당화법으로 이를 high fructose corn syrup(HFCS) 제조공정에 응용코져 연구하였다. 400g/$\ell$ 생전분을 24시간 무증자당화시켜 이성화반응에 적합한 고농도인 398g/$\ell$와 고순조인 98 포도당 용액을 농축과정을 거치지 않고 직접 얻을 수 있었다. 전분에 대한 포도당수율은 0.90으로, 미반응 잔유전분은 쉽게 원심분리되었으며, 재당화도 용이하였다. 또한 무증자당화액은 단백질성 변성물, 이온 등 불순물의 함량이 매우 적어, 이성화 반응 전단계인 분리 정제과정을 단순화할 수 있었다. 생성된 당용액의 고정화 포도당 이성화효소 기질로서의 적합성을 검토하여 우수한 결과를 얻었다. 생전분 직접당화법을 도입한 새로운 HFCS 제조공정은 액화/당화공정을 경유하는 기존의 공정에 비하여 여러가지 장점이 있어 산업적 활용이 기대되며, 이를 위한 후속연구가 요망된다.

• 식품산업과 영양
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• 제21권1호
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• pp.26-30
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• 2016

• 식품과학과 산업
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• 제47권3호
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• pp.39-49
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• 2014

• Journal of Dairy Science and Biotechnology
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• 제35권2호
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• pp.99-104
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• 2017

The global consumption of fructose in the form of high-fructose corn syrup (HFCS) has increased considerably over the past several decades. The current intake of HFCS exceeds that of other major caloric sweeteners such as sucrose. Fructose has potentially adverse effects on human metabolism. Therefore, the objective of this study was to analyze the monosaccharide contents of 13 sweetened milk-based beverages by using high-performance liquid chromatography (HPLC). The total monosaccharide (fructose and glucose) contents of various sweetened milk-based beverages ranged from 0.60 g/100 mL (cheese) to 26.03 g/100 mL (maple caramel snack), while milk showed only 0 g/100 mL monosaccharides. The fructose-to-glucose ratio of various popular sweetened milk-based beverages ranged from not-detected (ND) to 2.24 g/100 mL, but the content of glucose was higher than that of fructose in only three samples, namely, strawberry flavored milk, shrimp chips, and maple caramel snack. The analyzed results revealed important basic information about monosaccharides in various milk-based sweetened beverages, presenting ideas for future fructose research.

• 식품과학과 산업
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• 제51권1호
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• pp.45-60
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• 2018

The starch industry in Korea had been based on sweet potato and potato for long time to produce starches which were used for mainly starch noodle such as cellophane noodle. Because of the poor storage stability, high price, and fluctuation of production by year and year of potatoes, the raw material for the production of starch had been changed to corn in 1970s. Along with this, the mass production system had been established, which enabled the production of various starch-related products including modified starches for food, textile, paper, and other industrial uses, starch sweetners, high fructose corn syrup, and gelatinized starch. In this paper, a brief background of corn industry in Korea has been described. The production of starch from corn has been emphasized and the future of corn industry in relation with GMO has been suggested.

• 한국식품영양학회지
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• 제9권3호
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• pp.259-264
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• 1996

Five different liquid sugars, invert sugar, corn syrup, high fructose corn syrup, sorbitol and oligosaccharide were compared to improve the shelf life of sponge cake and to decrease the contamination of microorganisms in the baking plant. Sucrose was used as a control. The use of 40% of liquid sugar was more effective than that of 20% on the staling and softness. Invert sugar showed the lowest water activity and the highest water holding capacity. Oligosaccharide showed the highest water activity and the lowest water holding capacity among loquid sugars used. 20% of replacemint of sucrose with invert sugar decreased the contamination more than 100% of sucrose.

• BMB Reports
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• 제51권9호
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• pp.429-436
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• 2018

Fructose in the form of sucrose and high fructose corn syrup is absorbed by the intestinal transporter and mainly metabolized in the small intestine. However, excess intake of fructose overwhelms the absorptive capacity of the small intestine, leading to fructose malabsorption. Carbohydrate response element-binding protein (ChREBP) is a basic helix-loop-helix leucine zipper transcription factor that plays a key role in glycolytic and lipogenic gene expression in response to carbohydrate consumption. While ChREBP was initially identified as a glucose-responsive factor in the liver, recent evidence suggests that ChREBP is essential for fructose-induced lipogenesis and gluconeogenesis in the small intestine as well as in the liver. We recently identified that the loss of ChREBP leads to fructose intolerance via insufficient induction of genes involved in fructose transport and metabolism in the intestine. As fructose consumption is increasing and closely associated with metabolic and gastrointestinal diseases, a comprehensive understanding of cellular fructose sensing and metabolism via ChREBP may uncover new therapeutic opportunities. In this mini review, we briefly summarize recent progress in intestinal fructose metabolism, regulation and function of ChREBP by fructose, and delineate the potential mechanisms by which excessive fructose consumption may lead to irritable bowel syndrome.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.151-156
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• 2017

High-fructose corn syrup (HFCS) is widely used as sweetener, and its overconsumption is become a major health problem. In the present study, we used adult female rats and applied a 28 days HFCS feeding model to monitor the estrous cycle and changes in tissue weights and histology. Adult female rats were divided into three groups. Animals were fed with ad libitum normal chow and (1) 24 hours tap water (Control group), (2) 12 hours HFCS access during dark period and 12 hours tap water (12H group), and (3) 24 hours HFCS only access (24H group). Total exposure period was 28 days. There is no significant change in body weight between control and HFCS-fed animals. Both absolute and relative weights of ovary in 24H animals were significantly heavier than those in control or 12H animals. The absolute and relative weights of the kidney and liver in 24H groups were significantly heavier than those in control or 12H animals. The estrous cycles of the 24H animals were significantly longer. Histological analyses revealed that 24H ovaries were relatively bigger and possessed more corpus lutea than control ovaries. Uterine sections of 12H and 24H animals showed a well-developed stratum vasculare between inner and outer myometrial layers. The number of endometrial glands were decreased in 12H uteri, and recovered in 24H uteri compared to control. Numbers of convoluted tubule in distal region increased in 12H and 24H kidney samples. Liver specimens of 12H and 24H showed the increased number of fat containing vacuoles. In conclusion, our study demonstrated that HFCS treatment for 28 days could induce (1) changes in length of estrous cycle with extended estrous and diestrous stages, (2) altered ovarian and uterine histology, and (3) liver and renal lipid accumulation. These findings reveal the adverse effects of HFCS drinking on the reproductive function and lipid metabolism of female rats.

• Applied Biological Chemistry
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• 제26권4호
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• pp.222-230
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• 1983

본 연구에서는 저자등이 분리한 바 있는 Streptomyces griseolus가 분비한 xylose 이성화 효소(D-xylose ketol isomerase, EC 5.3.1.5, 포도당 이성화 효소)의 고정화 및 이 고정화 효소에 의한 파일롯트 규모의 이성화당 생산에의 응용을 시도하였다. 세포내 분비된 Xylose 이성화 효소를 함유한 미생물 균체를 호모 게나이저로 $500kg/cm^2$압력하에 파쇄한 결과 원 효소 역가의 98.8%가 얻어졌고, lysozyme으로 분해 했을 때는 54.7%가 얻어졌다. 이 효소의 고정화를 위한 담체로서는 Diaion HP 20, Duolite A-7, Amberlite IRA 93 및 94와 같은 포리스형 수지류가 효과적임이 밝혀졌고, Amberlite IRA 93에 대한 재생 형태는 $BO_4--$가, Diaion HP 20에 대해서는 $HCO_3-$가 효과적이었다. Amberline IRA 93에 대한 고정화 최적 조건은 pH 8.0에 $55^{\circ}C$로서 80.6%의 효소 고저오하 수율을 나타내었으며 효소 활성 반감기는 $65^{\circ}C$에서 24일 이상이었다. Amberlite IRA 93에 효소를 탈착(脫着) 실험한 결과, 담체는 재 사용이 가능 하였으며 2,3,4,5회째의 효소의 재 고정화율은 각각 98.2, 93.3, 90.7 및 87.5%를 나타내었다. 고정화 효소의 최적 반응 온도는 $60{\sim}70^{\circ}C$로 원효소의 경우에 비하여 다소 낮아지면서 폭이 넓어졌으며, 최적 pH는 $8.0{\sim}8.3$으로 알카리 쪽으로 이동하였다. 파일롯트 규모로 본 고정화 효소 충전탑(내경 30cm, 높이 85cm)에 의한 이성화당의 생산을 시도하였던바, 고정화 효소(350 IXIU/ml-R) 1리터가 30일동안에 약 293리터의 이성화당을 생산할 수 있는 것으로 나타났다.