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Characters of motile aeromonads isolated from imported ornamental fish (수입 관상어에서 분리한 motile aeromonads의 특성)

  • Jin, Se-yun;Ko, Chang-yong;Lee, Ye-ji;Jung, Yun-hee;Ju, Seong-cheol;Kim, Eunheui
    • Journal of fish pathology
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    • v.30 no.2
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    • pp.89-96
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    • 2017
  • The majority of freshwater ornamental fish are imported and distributed domestically, causing high risk of exposure to exotic pathogens and drug resistant bacteria in Korea. Aeromonas hydrophila is known as a common species of fresh water bacteria and opportunistic fish pathogen, as well as a species causing zoonotic infection. In this study, we isolated motile aeromonads from various imported freshwater ornamental fish and studied the characters of the isolates. Imported freshwater ornamental fish were purchased on day 1 after the fish were deposited in the aquarium. Bacteria were isolated from the liver, kidney and spleen of fish using 0.5% NaCl containing tryptic soy agar medium. Bacteria were grouped on the basis of their morphological characteristics. The colonies with clear zone on starch-ampicillin agar (SA agar) were tentatively identified as Aeromonas spp. Two hundred and twenty-six strains, about 70% of total isolates were assumed to be Aeromonas spp. Nine isolates were further identified based on the result of the API 20E test and PCR using primers specific for A. hydrophila 16S rRNA gene. The isolates were identified as A. hydrophila and the API 20E test showed differences in trisodium citrate, D-sucrose, D-melibiose, amygdalin and L-arabinose availability between the nine isolates and standard A. hydrophila. The susceptibilities of the isolated bacteria to 10 antibacterial agents were confirmed by the disk diffusion method. Isolated strains were found to be resistant to amoxicillin and ampicillin and sensitive to florfenicol. However, 7 isolates showed multiple drug resistances to erythromycin, oxytetracycline, nalidixic acid etc. Pathogenicity of the isolates was determined by the artificial challenge test on goldfish (Carassius auratus). Three isolates caused 60 ~ 80% mortality in goldfish within 5 days after the initiation of challenge. These results indicate that multiple drug resistant, highly pathogenic and exotic A. hydrophila can spread to domestic aquarium and the preventive treatment of fish before sale is necessary.

Regional Differentials in Mortality in Korea, 1990-2000 (사망력 수준의 시ㆍ군별 편차 및 그 변화 추이, 1990∼2000)

  • 김두섭;박효준
    • Korea journal of population studies
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    • v.26 no.1
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    • pp.1-30
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    • 2003
  • This paper attempts to explore the effects of ecological and socioeconomic factors on the level of mortality and the changing trends of such effects during the period of 1990∼2000. For this purpose the population census data and micro-data from the vital statistics for years 1990, 1995 and 2000 were used. As indicators of mortality, the crude death rate(CDR), the standardized death rate(SDR) and the longevity rate were calculated for 170 'Si' s and 'Gun's. Using GIS, this paper first presents the mortality and longevity maps for years 1990, 1995 and 2000. Then ANOVA and regression analyses are carried out in an effort to generalize the effects of ecological and socioeconomic factors on the CDR, the SDR and the longevity rate. When the mortality and longevity maps are examined, three indices of mortality are found to be markedly high in the southwest coastal regions of Cholla-Nam-Do. By contrast, Seoul and Pusan metropolitan areas show substantially low level of mortality and longevity in these indices. It is also found that the regional differentials in the SDR and the longevity rate show a trend of becoming smaller after 1990. The research, however, does not find any linear relationship between the SDR and the longevity rate. The causal mechanisms of the two indices are found to be different. The results of the ANOVA and the regression analysis reveal that the locational factors of both mountainous and farming regions tend to increase the CDR and SDR while both coastal and farming regions disclose a tendency of increasing the longevity rate. The level of statistical significance of these analytical results is found to be weaker when socioeconomic factors such as education, income, marital status, availability of medical care, and sanitary conditions of the region are taken into account. The regional differentials in the mortality level seem to have a clear relationship not only with the socioeconomic factors but also with the age structure influenced by the age selectivity of migration during the past 40 years.

Long-Term Sludge Application on Extractable Contents of Copper in Soils (Sludge 를 장기처리(長期處理)한 토양중(土壤中)에서 추출물질종류(抽出物質種類)에 따른 Cu 의 함량(含量))

  • Kim, Seong-Jo;Baek, Seung-Hwa;Han, Goang-Lae
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.2
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    • pp.116-121
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    • 1989
  • An experiment on movements of copper in soils was conducted to evaluate the effectiveness of several soil extractants in predicting to be possibly the plant availability of copper in soils. Soil samples were taken six, from Las Virgines loam that the samples naturally included high contents of heavy metals near Los Angeles, Greenfield sandy loam and IDomino Loam that received 22.5, and $45.0mg.\;ha^{-1},\;yr^{-1}$ of composted sludge from Los Angeles from 1975 to 1983, respectively. Copper in soils were extracted with $4M\;HNO_3$, 0.1M HCl, 0.5M DTPA-0.1M TEA, 0.5M EDTA-0.1M $Ca(NO_3)_2$, and 1M ammonium acetate(pH 7), and analyzed by atomic absorption spectroscopy. The results were as follows: 1. The total copper contents in soil that received $45.0mg.\;ha^{-1},\;yr^{-1}$ of composted sludge were higher than that received $22.5mg.\;ha^{-1}.yr^{-1}$, regardless of soil samples. 2. The ratios of extractable copper contents with EDTA, DTPA, 0.1M HCl and 1M ammonium acetate to total copper contents extracted with 4M $HNO_3$ of long-term sludge applications were larger than those of natural, sludge-nontreated soils. 3. Statistically significant increase in total copper contents was found in the increasing values of multipling CEC, or OM% by R, ratio of extractable copper contents to those by 4M $HNO_3$ extraction as total copper contents in soils.

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Physical Properties and Apparent Thermal Diffusivity of the Soils where Soil Temperature is Measured Regularly (기상청(氣象廳) 지온(地溫) 측정(測定) 토양(土壤)의 물리적(物理的) 성질(性質)과 겉보기 열확산(熱擴散) 계수(係數) 산정(算定))

  • Song, Kwan-Cheol;Jung, Yeong-Sang;Kim, Byung-Chan;Ahn, Yoon-Soo;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.3
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    • pp.220-230
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    • 1992
  • Soil temperature is one of the important environmental factors which control all the physical, chemical and biological processes in soil including germination and root growth of plants and other organisms living in the soil ecosystem. Soil water and nutrient availability and mobility are temperature dependent. Soil temperature change is depended primarily upon energy exchange in soil surface, meteorological variance and physical properties of the soils which are closely related to heat transfer mechanism. In this study physical properties including bulk density, soil texture and organic matter content were measured and thermal diffusivity on the soils was calculated. Soil samples from the 66 meteorological stations under the Korea Meteorology were collected and the physical parameters were measured. To obtain relationship between thermal diffusivity and soil water content a heat probe thermal diffusivity measurement apparatus was designed and used in this experiment. According to the survey on soil physicsal properties on the 66 meteorological stations, the 52% of the surface soil texture were sandy loam and laomy sand or sand, 38% were loam and silty loam, and 10% were clay loam and silty clay loam. The bulk density which was closely related with thermal properties showed average of $1.41g/cm^3$ for sandy soils, $1.33g/cm^3$ for loam and silty loam soils, and $1.21g/cm^3$ for clay loam and silty clay loam soils. The apparent thermal diffusivity of the upper layer from 0 to 30cm ranged from 1.16 to $8.40{\times}10^{-3}cm^3/sec$ with average of $3.53{\times}10^{-3}cm^3/sec$. The apparent thermal diffusivities of the Jeju soils of which organic matter contents were high and the bulk densities were low were near $2{\times}10^{-3}cm^3/sec$. The thermal diffusivity of snow measured in Chuncheon ranged from 0.822 to $2.237{\times}10^{-3}cm^3/sec$. The damping depth calculated from the thermal diffusivity ranged from 5.92 to 13.65cm for daily basis and 124 to 342cm for yearly basis. The significant regression equation to estimate thermal diffusivity with bulk density and soil water content was obtained by the heat probe in laboratory.

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Performance of Drip Irrigation System in Banana Cultuivation - Data Envelopment Analysis Approach

  • Kumar, K. Nirmal Ravi;Kumar, M. Suresh
    • Agribusiness and Information Management
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    • v.8 no.1
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    • pp.17-26
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    • 2016
  • India is largest producer of banana in the world producing 29.72 million tonnes from an area of 0.803 million ha with a productivity of 35.7 MT ha-1 and accounted for 15.48 and 27.01 per cent of the world's area and production respectively (www.nhb.gov.in). In India, Tamil Nadu leads other states both in terms of area and production followed by Maharashtra, Gujarat and Andhra Pradesh. In Rayalaseema region of Andhra Pradesh, Kurnool district had special reputation in the cultivation of banana in an area of 5765 hectares with an annual production of 2.01 lakh tonnes in the year 2012-13 and hence, it was purposively chosen for the study. On $23^{rd}$ November 2003, the Government of Andhra Pradesh has commenced a comprehensive project called 'Andhra Pradesh Micro Irrigation Project (APMIP)', first of its kind in the world so as to promote water use efficiency. APMIP is offering 100 per cent of subsidy in case of SC, ST and 90 per cent in case of other categories of farmers up to 5.0 acres of land. In case of acreage between 5-10 acres, 70 per cent subsidy and acreage above 10, 50 per cent of subsidy is given to the farmer beneficiaries. The sampling frame consists of Kurnool district, two mandals, four villages and 180 sample farmers comprising of 60 farmers each from Marginal (<1ha), Small (1-2ha) and Other (>2ha) categories. A well structured pre-tested schedule was employed to collect the requisite information pertaining to the performance of drip irrigation among the sample farmers and Data Envelopment Analysis (DEA) model was employed to analyze the performance of drip irrigation in banana farms. The performance of drip irrigation was assessed based on the parameters like: Land Development Works (LDW), Fertigation costs (FC), Volume of water supplied (VWS), Annual maintenance costs of drip irrigation (AMC), Economic Status of the farmer (ES), Crop Productivity (CP) etc. The first four parameters are considered as inputs and last two as outputs for DEA modelling purposes. The findings revealed that, the number of farms operating at CRS are more in number in other farms (46.66%) followed by marginal (45%) and small farms (28.33%). Similarly, regarding the number of farmers operating at VRS, the other farms are again more in number with 61.66 per cent followed by marginal (53.33%) and small farms (35%). With reference to scale efficiency, marginal farms dominate the scenario with 57 per cent followed by others (55%) and small farms (50%). At pooled level, 26.11 per cent of the farms are being operated at CRS with an average technical efficiency score of 0.6138 i.e., 47 out of 180 farms. Nearly 40 per cent of the farmers at pooled level are being operated at VRS with an average technical efficiency score of 0.7241. As regards to scale efficiency, nearly 52 per cent of the farmers (94 out of 180 farmers) at pooled level, either performed at the optimum scale or were close to the optimum scale (farms having scale efficiency values equal to or more than 0.90). Majority of the farms (39.44%) are operating at IRS and only 29 per cent of the farmers are operating at DRS. This signifies that, more resources should be provided to these farms operating at IRS and the same should be decreased towards the farms operating at DRS. Nearly 32 per cent of the farms are operating at CRS indicating efficient utilization of resources. Log linear regression model was used to analyze the major determinants of input use efficiency in banana farms. The input variables considered under DEA model were again considered as influential factors for the CRS obtained for the three categories of farmers. Volume of water supplied ($X_1$) and fertigation cost ($X_2$) are the major determinants of banana farms across all the farmer categories and even at pooled level. In view of their positive influence on the CRS, it is essential to strengthen modern irrigation infrastructure like drip irrigation and offer more fertilizer subsidies to the farmer to enhance the crop production on cost-effective basis in Kurnool district of Andhra Pradesh, India. This study further suggests that, the present era of Information Technology will help the irrigation management in the context of generating new techniques, extension, adoption and information. It will also guide the farmers in irrigation scheduling and quantifying the irrigation water requirements in accordance with the water availability in a particular season. So, it is high time for the Government of India to pay adequate attention towards the applications of 'Information and Communication Technology (ICT) and its applications in irrigation water management' for facilitating the deployment of Decision Supports Systems (DSSs) at various levels of planning and management of water resources in the country.

Effects of Medium and Planting Density on Growth and Yield of Seed Potatoes Grown in a Wick Hydroponic System (배지 및 재식밀도가 심지양액재배 씨감자의 생육 및 수량에 미치는 영향)

  • Kim, Chan-Woo;Song, Chang-Khil;Park, Jung-Sik;Mun, Hyun-Ki;Kang, Young-Kil;Kang, Bong-Kyoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.3
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    • pp.251-255
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    • 2008
  • This study was carried out in 2002 to determine the usefulness of Jeju scoria for a component of a growth medium and optimum planting density of 'Dejima' seed potatoes (Solanum tuberosum L.) grown in a wick hydroponic system. The minitubers ($7.0{\pm}0.2\;g$) produced in an aeroponics system were planted at five planting densities (3 to 15 tubers/box; 19 to $95\;tubers/m^2$) in polystyrene boxes (51 cm long $\times$ 31 cm wide $\times$ 20 cm high) containing two media (perlite + peatmoss and Jeju scoria + peatmoss 1:2, v/v mixtures). There were no significant interactions between medium and planting density for the growth and tuber yield traits. Shoot growth and the number of tubers per plant were not significantly affected by the media. However, tuber yield was higher in the perlite + peatmoss mixture than in the Jeju scoria + peatmoss mixture. The percentage of underdeveloped plants ranged from 8.3 to 14.7% at four lower planting densities (3 to 12 tubers/ box), and was 25.8% at the highest planting density (15 tubers/box). As planting density was increased from 3 to 15 tubers per box, seed potato (${\geq}5\;g$ tuber) number increased from 101 to 269 and yield from 6.3 to $11.6\;kg/m^2$. These results indicate that the perlite + peatmoss mixture might be more suitable for seed potato production in the wick hydroponic system, and considering the percentage of underdeveloped plants and tuber yield, optimum planting density would be 56 to $76\;tubers/m^2$ in the system depending on availability of seed potatoes.

Population Structure and Habitat Characteristics of Deutzia paniculata Nakai, as an Endemic Plant Species in Korea (한반도 특산식물 꼬리말발도리 개체군 구조 및 서식지 특성)

  • Jung, Ji-young;Pi, Jung-hun;Park, Jeong-geun;Jeong, Mi-jin;Kim, Eun-hye;Seo, Gang-Uk;Lee, Cheul-ho;Son, Sung-won
    • Korean Journal of Ecology and Environment
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    • v.49 no.1
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    • pp.31-41
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    • 2016
  • Deutzia paniculata is an endemic species to the Korean Peninsula. Despite of importance for conservation, the population structure and habitat characteristics of D. paniculata have not been determined yet. We analyzed the ecological characteristics of the species based on the literature review and field survey. Field survey was conducted on May to October 2014 during which 11 quadrats of size $15{\times}15m$ were studied in six regions. Each of the quadrats were further divided into $5{\times}5m$ small quadrats and population characteristics were recorded. The population and habitat characteristics were analyzed, including species abundance (density and coverage), demographic attributes (flowering rates and fruiting plants), vegetation (structure, species composition), light availability (transmitted light and canopy openness) and soil characteristics (temperature and humidity). We found that D. paniculata mainly distributed in Gyeongsangdo (including Taebaek in Gangwondo) along a broad elevational range of 290~959 m (mean: 493 m) above sea level. In preferred habitat the species grows within the slope range of $7^{\circ}$ and $35^{\circ}$ with the average of $16^{\circ}$. D. paniculata was generally distributed on talus deposits and low adjacent slopes. The average number of individual plants per small quadrat was 12.5 with the mean density $0.5stems\;m^{-2}$. The vegetative reproduction was frequent in D. paniculata and mean flowering rate was as low as 15%. Altogether 138 taxa were found in whole observation area with the dominant tree species mainly spring ephemerals, such as Cornus controversa (importance value: 25.5%) and Fraxinus rhynchophylla (importance value: 15.8%). Although, C. controversa usually grows on steep slopes and F. rhynchophylla mostly distributed at high-altitudes, however, both species distributed in disturbed environments and among talus deposits. Thus based on our results, we concluded that D. paniculata is a disturbance-prone species, primarily existing in habitats subjected to natural disturbances, such as floods. The species occurs less at anthropogenically disturbed sites, thus there is no apparent threat to the populations and habitat of D. paniculata.

Effects of Compost Leachate on Growth and Yield of Leaf Lettuce in Hydroponic Culture (퇴비단 여과액비가 수경재배에서 상추의 생육과 수량에 미치는 영향)

  • Ryoo, Jong-Won
    • Journal of Animal Environmental Science
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    • v.15 no.1
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    • pp.51-58
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    • 2009
  • The compost leachate was dark-colored solution that leaches out of the bottom of the compost pile. The compost leachate was rich in nutrients and can potentially used in plant culture. In the organic production, commercial liquid fertilizer was used to insure the availability of nutrients during the formation of the yield. The cost of supplemental liquid fertilizer could be reduced by developing a fertilizer based on animal fertilizer. This experiment was conducted to investigate the effect of different combination of compost leachate and conventional inorganic solution in hydroponic culture for lettuce growth. Six different treatments were applied. The compost leachate(CL) and nutrient solution(NS) were mixed by six different mixing ratios of 0:100, 20:80, 40:60, 60:40%, 80:20 and 100%:0% based on nitrogen content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of leaf lettuce. The concentration of nutrient solution was adjusted 1.5 mS/cm in EC. The compost leachate was low in phosphorus(P), calcium(Ca), magnesium(Mg), but rich in potassium(K). The plant height of lettuce treated with CL 20+NS 80% was similar with 100% NS of control plot. Plant height was highest in the plot of CL 20+NS 80%. The treatment of 100% compost leachate was lowest in the growth characteristics of leaf lettuce. Number of leaf was very low in 100% compost leachate compared with plot of chemical nutrient solution. In the beginning of growth stage, SPAD value was reduced in plot treated with CL100%, but CL20+NS80% plot was higher compared to 100% compost leachate. SPAD value of leaf lettuce leaves was decreased as the amount of CL was increased. The dry weight of lettuce were 107.4, 104.2g in plot of NS 100% and CL 20%+NS80%, respectively. The leaf number and plant weight were decreased at high application plots of compost leachate. The leaf lettuce showed lowest in the plot treated with 100% compost leachate, and the growth of lettuce severely decreased after application of 100% CL treatment. The results showed that compost leachate can be use as liquid fertilizer for the organic hydroponic production. The mixture solution of 20% of compost leachate and 80% of nutrient solution could be used as a nutrition solution in hydroponic culture of leaf lettuce. Our studies have shown that is possible to produce using compost leachate, although growth is slower than when using a conventional inorganic hydroponic solution.

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Simultaneous Multiple Transmit Focusing Method with Orthogonal Chirp Signal for Ultrasound Imaging System (초음파 영상 장치에서 직교 쳐프 신호를 이용한 동시 다중 송신집속 기법)

  • 정영관;송태경
    • Journal of Biomedical Engineering Research
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    • v.23 no.1
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    • pp.49-60
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    • 2002
  • Receive dynamic focusing with an array transducer can provide near optimum resolution only in the vicinity of transmit focal depth. A customary method to increase the depth of field is to combine several beams with different focal depths, with an accompanying decrease in the frame rate. In this Paper. we Present a simultaneous multiple transmit focusing method in which chirp signals focused at different depths are transmitted at the same time. These chirp signals are mutually orthogonal in a sense that the autocorrelation function of each signal has a narrow mainlobe width and low sidelobe levels. and the crossorelation function of any Pair of the signals has values smaller than the sidelobe levels of each autocorrelation function. This means that each chirp signal can be separated from the combined received signals and compressed into a short pulse. which is then individually focused on a separate receive beamformer. Next. the individually focused beams are combined to form a frame of image. Theoretically, any two chirp signals defined over two nonoverlapped frequency bands are mutually orthogonal In the present work. however, a tractional overlap of adjacent frequency bands is permitted to design more chirp signals within a given transducer bandwidth. The elevation of the rosscorrelation values due to the frequency overlap could be reduced by alternating the direction of frequency sweep of the adjacent chirp signals We also observe that the Proposed method provides better images when the low frequency chirp is focused at a near Point and the high frequency chirp at a far point along the depth. better lateral resolution is obtained at the far field with reasonable SNR due to the SNR gain in Pulse compression Imaging .

The Effectiveness of Spiral Computed Tomography as a Diagnostic Tool in Pulmonary Embolism(Comparison of Spiral CT with Ventilation-Perfusion Scan) (폐색전증 진단의 도구로서의 Spiral Computed Tomography의 유용성(폐환기관류주사와의 비교))

  • Koh, Jae-Hyun;Oh, Eun-Young;Park, Jung-Ho;Park, Sang-Joon;Yun, Jung-Hwan;Park, Jung-Woong;Suh, Gee-Young;Chung, Man-Pyo;Lee, Kyung-Soo;Kwon, O-Jung;Rhee, Chong-H.
    • Tuberculosis and Respiratory Diseases
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    • v.46 no.4
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    • pp.564-573
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    • 1999
  • Background: With variable symptoms and nonspecific radiographic appearances, pulmonary embolism (PE) is a frequent and often undiagnosed cause of mortality and morbidity. The Prospective Investigation of Pulmonary Embolism Diagnosis (PIOPED) study suggested that the majority of patients undergoing ventilation-perfusion (V-Q) scan would require additional studies to establish or to exclude the diagnosis of PE. Pulmonary angiography has been regarded as gold standard for diagnosis of PE. However, it is an invasive procedure that may be associated with significant notable morbidity and mortality. Thus, availability of an accurate, noninvasive screening examination is highly desirable. Method: From October 1994 to February 1997, twenty patients (male 13, female 7, range 23-91 years, median 58 years) who were suspected as pulmonary embolism on the basis of clinical evidence and underwent the spiral volumetric computed tomography (spiral CT), were studied retrospectively to evaluate the effectiveness of spiral CT as a diagnostic tool in PE. Results: PE could be excluded with spiral CT in 4 patients ; diagnoses of these patients were lung cancer, pneumonia with lung abscess, bilateral pleural effusion due to congestive heart failure, nonspecific pulmonary abnormality retrospectively. One patient who disclosed high probability in V/Q scan, could be diagnosed as pneumonia with lung abscess and underlying emphysema with spiral CT. Among 4 patients who showed intermediate and low probability in V/Q scan, 3 patients could be confirmed as PE with spiral CT. Spiral CT was helpful in 3 patients, in whom V/Q scan could not be performed due to other reasons (e.g. night time, mechanical ventilation) to confirm the diagnosis of PE. Spiral CT could demonstrate embolus above lobar artery level in 11 patients, and up to segmental artery level in 5 patients. Conclusion: This study demonstrated that spiral CT could allow accurate demonstration of thrombotic clots in centrally localized embolism. Spiral CT could be effective, specific, noninvasive and useful diagnostic screening modality for the diagnosis of pulmonary embolism.

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