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http://dx.doi.org/10.7847/jfp.2017.30.2.089

Characters of motile aeromonads isolated from imported ornamental fish  

Jin, Se-yun (Department of Aqualife Medicine, Chonnam National University)
Ko, Chang-yong (Department of Aqualife Medicine, Chonnam National University)
Lee, Ye-ji (Department of Aqualife Medicine, Chonnam National University)
Jung, Yun-hee (Department of Aqualife Medicine, Chonnam National University)
Ju, Seong-cheol (Department of Aqualife Medicine, Chonnam National University)
Kim, Eunheui (Department of Aqualife Medicine, Chonnam National University)
Publication Information
Journal of fish pathology / v.30, no.2, 2017 , pp. 89-96 More about this Journal
Abstract
The majority of freshwater ornamental fish are imported and distributed domestically, causing high risk of exposure to exotic pathogens and drug resistant bacteria in Korea. Aeromonas hydrophila is known as a common species of fresh water bacteria and opportunistic fish pathogen, as well as a species causing zoonotic infection. In this study, we isolated motile aeromonads from various imported freshwater ornamental fish and studied the characters of the isolates. Imported freshwater ornamental fish were purchased on day 1 after the fish were deposited in the aquarium. Bacteria were isolated from the liver, kidney and spleen of fish using 0.5% NaCl containing tryptic soy agar medium. Bacteria were grouped on the basis of their morphological characteristics. The colonies with clear zone on starch-ampicillin agar (SA agar) were tentatively identified as Aeromonas spp. Two hundred and twenty-six strains, about 70% of total isolates were assumed to be Aeromonas spp. Nine isolates were further identified based on the result of the API 20E test and PCR using primers specific for A. hydrophila 16S rRNA gene. The isolates were identified as A. hydrophila and the API 20E test showed differences in trisodium citrate, D-sucrose, D-melibiose, amygdalin and L-arabinose availability between the nine isolates and standard A. hydrophila. The susceptibilities of the isolated bacteria to 10 antibacterial agents were confirmed by the disk diffusion method. Isolated strains were found to be resistant to amoxicillin and ampicillin and sensitive to florfenicol. However, 7 isolates showed multiple drug resistances to erythromycin, oxytetracycline, nalidixic acid etc. Pathogenicity of the isolates was determined by the artificial challenge test on goldfish (Carassius auratus). Three isolates caused 60 ~ 80% mortality in goldfish within 5 days after the initiation of challenge. These results indicate that multiple drug resistant, highly pathogenic and exotic A. hydrophila can spread to domestic aquarium and the preventive treatment of fish before sale is necessary.
Keywords
Imported ornamental fish; Motile aeromonads; Multiple drug resistance;
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