• The Journal of the Acoustical Society of Korea
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• v.35 no.1
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• pp.74-82
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• 2016

In this paper, a new automatic speech recognition (ASR) was proposed where ultrasonic doppler signals were used, instead of conventional speech signals. The proposed method has the advantages over the conventional speech/non-speech-based ASR including robustness against acoustic noises and user comfortability associated with usage of the non-contact sensor. In the method proposed herein, 40 kHz ultrasonic signal was radiated toward to the mouth and the reflected ultrasonic signals were then received. Frequency shift caused by the doppler effects was used to implement ASR. The proposed method employed multi-channel ultrasonic signals acquired from the various locations, which is different from the previous method where single channel ultrasonic signal was employed. The PCA(Principal Component Analysis) coefficients were used as the features of ASR in which hidden markov model (HMM) with left-right model was adopted. To verify the feasibility of the proposed ASR, the speech recognition experiment was carried out the 60 Korean isolated words obtained from the six speakers. Moreover, the experiment results showed that the overall word recognition rates were comparable with the conventional speech-based ASR methods and the performance of the proposed method was superior to the conventional signal channel ASR method. Especially, the average recognition rate of 90 % was maintained under the noise environments.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.141-141
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• 2017

The ubiquitin-proteasome pathway is the major regulatory mechanism in a number of cellular processes for selective degradation of proteins and involves three steps: (1) ATP dependent activation of ubiquitin by E1 enzyme, (2) transfer of activated ubiquitin to E2 and (3) transfer of ubiquitin to the protein to be degraded by E3 complex. F-box proteins are subunit of SCF complex and involved in specificity for a target substrate to be degraded. F-box proteins regulate many important biological processes such as embryogenesis, floral development, plant growth and development, biotic and abiotic stress, hormonal responses and senescence. However, little is known about the F-box genes in wheat. The draft genome sequence of wheat (IWGSC Reference Sequence v1.0 assembly) used to analysis a genome-wide survey of the F-box gene family in wheat. The Hidden Markov Model (HMM) profiles of F-box (PF00646), F-box-like (PF12937), F-box-like 2 (PF13013), FBA (PF04300), FBA_1 (PF07734), FBA_2 (PF07735), FBA_3 (PF08268) and FBD (PF08387) domains were downloaded from Pfam database were searched against IWGSC Reference Sequence v1.0 assembly. RNA-seq paired-end libraries from different stages of wheat, such as stages of seedling, tillering, booting, day after flowering (DAF) 1, DAF 10, DAF 20, and DAF 30 were conducted and sequenced by Illumina HiSeq2000 for expression analysis of F-box protein genes. Basic analysis including Hisat, HTseq, DEseq, gene ontology analysis and KEGG mapping were conducted for differentially expressed gene analysis and their annotation mappings of DEGs from various stages. About 950 F-box domain proteins identified by Pfam were mapped to wheat reference genome sequence by blastX (e-value < 0.05). Among them, more than 140 putative F-box protein genes were selected by fold changes cut-offs of > 2, significance p-value < 0.01, and FDR<0.01. Expression profiling of selected F-box protein genes were shown by heatmap analysis, and average linkage and squared Euclidean distance of putative 144 F-box protein genes by expression patterns were calculated for clustering analysis. This work may provide valuable and basic information for further investigation of protein degradation mechanism by ubiquitin proteasome system using F-box proteins during wheat development stages.