• Korean Journal of Poultry Science
• /
• v.27 no.3
• /
• pp.227-233
• /
• 2000

Preset study was carried out to evaluate characteristics of lactic acid producing bacteria(LAB) in hen's cecum as probiotics value. Distribution of LAB in intestinal tracts was investigated using 5∼25 weeks - old hens. So, 12 strains to LAB with different morphology were isolated purely. Acid tolerance of LAB tested at pH 1, 2, 3, and 4, and bile resistant also tested at 0, 0.3% and 0.5% bile salt concentration. Growth pattern of LAB observed to 60h. All strains of cecal LAB couldn't survive at pH 1, and decreased linearly survival colony after incubation at pH 2 although some strains could survive for 2h. Most of LAB maintained constant number at pH 3 and 4. The bacterial action could increase linearly at 0% bile salt concentration in all of tested strains. However, only one strain could multiply at 0.3% bile salt, others were influenced by bile salt. That tendency was similar at 0.5% bile salt. Growth was peaked at 12 to 18 h after innoculation. After peak, the decreasing pattern of colony was different to strains which some strains decreased rapidly or maintained for long time. The LAB of hen's cecum was similar to intolerance acidity, but different to resistant to bile salt and growth pattern by strain. So, we choose three strains which have probiocs value, and identified as Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9 and Lactobacillus vaginalis LLA11.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.11
• /
• pp.1605-1609
• /
• 2001

A total of 252, 50 week-old Isa-brown laying hens were randomly allotted to 7 groups of 3 replicates. Mustard meal (MM) which is a by-product from mustard processing plant, was dried under the sun or in a gas heated pan. It contained on DM basis 30-32% CP, 19-22% EE and 12-13% CF. The meal from either drying method was incorporated into the diets at 0, 10, 20 and 30% which was equivalent to the substitution levels for soybean meal at 0, 31, 63 and 94%, respectively. All birds were individually kept in battery cages where feed and water were freely accessed throughout 84 days experimental period. It was found that egg production, feed intake, body weight gain and egg weight significantly decreased with the increased MM level. The inclusion of 20% MM did not show a significant difference in egg production and quality from the control, but produced 6-8% lower egg production. Feed intake was linearly decreased with the MM levels, except the 10% sun dried MM group. Fat deposition of the birds fed MM diets significantly decreased, while kidney weight increased when compared with the control group. However, the weight of thyroid glands and spleen trended to be heavier in the MM groups, but this was not significantly different among dietary treatments. It was concluded, MM from both drying methods could be incorporated in laying hen diets at the level of 10% without any adverse effect.

• Asian-Australasian Journal of Animal Sciences
• /
• v.5 no.2
• /
• pp.225-228
• /
• 1992

The study was undertaken to determine the effect of various levels of dietary magnesium on the metabolizability of magnesium in laying Tsaiya ducks and Leghorn hens. Twenty-five Tsaiya ducks and 25 Leghorn hens were raised in individual cages. The basal diet was mainly consisted of corn starch and isolated soybean protein. At the beginning of the experiments, birds were fed the basal diet added with 500 mg/kg Mg (1070 mg/kg in total by analysis) for 10 days in order to make the birds adapt to the new diet. Then, they were randomly divided into five treatments with five birds for each treatment and fed the experimental diets containing 690, 1070, 1690, 2150 or 2380 mg/kg Mg for 21 days and during the last five days excreta were collected. The experiment was repeated three times each at the age of 25, 31 and 36 weeks. The metabolizability of magnesium in the diet was determined by the indicator method. The metabolizabilities of magnesium for Tsaiya duck and Leghorn hen were 59.9% and 62.5%, respectively as the diet contained 690 mg/kg magnesium, and were significantly decreased (p<0.05) to 39.7 and 30.7%, respectively when the dietary magnesium content was increased to 2380 mg/kg.

• KSBB Journal
• /
• v.18 no.3
• /
• pp.202-206
• /
• 2003

A study of hen egg lysozyme extraction using reversed micelles and pressurized CO₂ phase was conducted. The relationship between the lysozyme extraction and water content (W/sub 0/) under the pressurized CO₂ conditions was investigated. The water content of the micellar organic phase was a significant parameter affecting the mass transfer of protein and enzymatic activity in reversed micellar process. It was found that the reversed micelles in the organic phase with pressurized CO₂ were larger than the organic phase without CO₂. Therefore, the extractionrate of lysozyme in the interface of the aqueous phase and the organic phase was increased. W/sub 0/ value was increased at the high surfactant concentration and the extraction rate of lysozyme was enhanced.

• Korean Journal of Poultry Science
• /
• v.27 no.2
• /
• pp.133-153
• /
• 2000

The egg's interior quality is one of the most important criteria for commercial producers and consumers. Internal quality is complex, including aesthetic factors such as taste, freshness, nutritional and processing values, and the genetic influences upon these upon these factors ranges from none to considerable. The rate of cholesterol synthesis in the hen is very high compared to other animals and humans. Genetic selection, diet drugs and other chemicals can alter cholesterol concentration in the plasma of laying hen, but attempts to manipulate the cholesterol concentration in the egg yolk are generally unsuccessful since the cholesterol can only be changed to a small extent. Factors which may affect the degree of pigmentation of the yolk include the type of xanthophyll and its concentration in the feed, the feed composition, and the health of the hen. Several feed ingredients interact with carotenoid pigment to improve or reduce their deposition rates in yolks. Egg weight is determined by genetics, body size prior to first egg housing density, environmental temperature, lighting program, total feed consumption, calcium, phosphorus, niacin, water, methionine, total sulfur amino acids, energy, linoleic acid, fat and protein levels. Eggs need to be promote levels. Eggs need to be promoted a versatile commodity and new processed egg items need to be developed. Organic eggs are laid by hens which were raised in chemical and drug frdd environments. There are still difficulties in producing these eggs due to the availability of organic poultry feeds and cost of organic grains.

• Korean Journal of Veterinary Research
• /
• v.58 no.4
• /
• pp.193-199
• /
• 2018

As animal welfare issue becomes important, the European Union bans conventional cages for laying hens from 2012. So the alternative housing systems like floor pens, aviaries or free range systems have been suggested. From 2011 to 2014, we monitored 20 welfare-oriented laying hen farms in South Korea to figure out serological status of major viral diseases. During this period, total 3,219 blood samples were collected from the randomly selected chickens to test and evaluate the hemagglutination inhibition titers for low pathogenic avian influenza, Newcastle disease and egg drop syndrome '76. A total of 2,926 blood samples were tested through enzyme linked immunosorbent assay (ELISA) to assess the serological status of infectious bronchitis (IB). The distribution of ELISA titers for IB was various from almost 0 to 20,000 through the all weeks of age. Also, the antibody coefficient of variation for most of the diseases in this study was higher than those of typical cage layers. As this study was the first surveillance for major avian viral diseases of the animal welfare-oriented farms in South Korea, the results obtained from this study will help to determine what information and resources are needed to maintain better biosecurity and to improve the health and welfare of laying hen flocks.

• Food Science of Animal Resources
• /
• v.41 no.5
• /
• pp.816-825
• /
• 2021

This study aims to validate a fast method of simultaneous analysis of 365 LCamenable and 142 GC-amenable pesticides in hen eggs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS), respectively, operating in multiple reaction monitoring (MRM) acquisition modes. The sample preparation was based on quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction. Key method performance parameters investigated were specificity, linearity, limit of quantification (LOQ), accuracy, precision and measurement uncertainty. The method was validated at two spiking levels (10 and 50 ㎍/kg), and good recoveries (70%-120%) and relative standard deviations (RSDs) (≤20) were achieved for 92.9% of LC-amenable and 86.6% of GC-amenable pesticide residues. The LOQs were ≤10 ㎍/kg for 94.2% of LC-amenable and 92.3% of GC-amenable pesticides. The validated method was further applied to 100 egg samples from caged hens, and none of the pesticides was quantified.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
• /
• v.33 no.2
• /
• pp.48-54
• /
• 2022

Objectives: Among adolescent development tasks, being independent of parents is an essential process for emotional and physical separation. There are many conflicts of separation and individuation between parents and adolescents; however, most clinicians explore the process of separation and individuation only from adolescents' perspective. Whether simultaneously or sequentially, separation-individuation occurs between adolescents and parents, respectively. The authors have already introduced the theory of synchronized individuation in a clinical case to explain the concept of this intersubjective phenomena. This study also attempts to prove the synchronized individuation theory through the interaction of characters in a movie and a fairy tale. Methods: The authors present the basis for the theory of synchronized individuation of adolescence through the growing process of Mason Junior, the main character of the movie "Boyhood," and from the process of the separation of a hen, Sprout, and an orphaned duckling in "The hen who dreamed she could fly." Results: Synchronized individuation was developed and observed from Mason, the son's perspective in "Boyhood," and Sprout, the mother's subjective perspective in the story of the hen. Conclusion: Increasing conflict and mutual impact in the relationship between adolescents and parents, ambivalent feelings for separation, selective identification of each other, mutual respect through mutual recognition, and role changes in relationship position were confirmed. Sons and mothers were individualized with synchronization.

• Korean Journal of Veterinary Service
• /
• v.31 no.3
• /
• pp.385-395
• /
• 2008

This study was carried out to investigate the residue level of fluoroquinolones in hen's general eggs and specific eggs by microbiological assay method and high performance liquid chromatography (HPLC) method. HPLC separation was carried out by reversed phase chromatography on a Symmetry $C_{18}$ (250${\times}$4.6 mm, $5{\mu}m$ particle size) with a phase composed of distilled water (containing 0.4% triethylamine and phosphoric acid) : Methanol (780 : 220, v/v), pumped isocratically at a flow rate of 1.0ml/min. A fluorescence detector was utilized with an excitation wavelength of 278nm and an emission wavelength of 456nm. The calibration curves were linear $({\gamma}^2{\geq}0.999)$ over a concentration range of $0.025{\sim}0.4{\mu}g/ml$. Average recoveries of the five fluoroquinolones in whole eggs at fortified levels of $0.05{\sim}0.2{\mu}g/g$ were ranged mean $78.1{\sim}91.7%$ and low coefficient of variation was less than 10% for all analysed samples. The limits of detection and limits of quantification for whole eggs were $1.2{\sim}6.0ng/g$ and $2.3{\sim}9.1ng/g$, respectively. Only one hen's general eggfrom chicken farm in Incheon was detected with the residual fluoroquinolones (Microbiological assay method; 1 of 47 general eggs) ; the range of residual concentration enrofloxacin was 0.12ppm. Those in food stores were detected with the residual fluoroquinolones (Microbiological assay method; 4 of 88 general eggs) ; the ranges of residual concentration enrofloxacin were $0.15{\sim}2.2 ppm$, ciprofloxacin $0.01{\sim}0.06ppm$, and hen's specific eggs (40) in food stores were not detected. For the microbiological assay method of fluoroquinolones in hen's eggs, as the results of comparative analysis, the disc diffusion method with E coli may be a little highly detected for the residual fluoroquinolones.

• Journal of Animal Science and Technology
• /
• v.51 no.5
• /
• pp.421-426
• /
• 2009

This study was conducted to clarify the characteristics of vocalization of laying hen related with space in battery cage. The size of cages were classified into control (0.30 m ${\times}$ 0.14 m ${\times}$ 0.55 m, length ${\times}$ width ${\times}$ height), small (0.21 m ${\times}$ 0.14 m ${\times}$ 0.55 m) and large (0.30 m ${\times}$ 0.30 m ${\times}$ 0.55 m) size. Vocalization of 16 individuals of laying hen in each group of Hy-Line Brown (80 week old) were recorded 3 hours per day (10:00am~11:00am, 3:00pm~4:00pm and 7:00pm~8:00pm) using digital recorder and microphone during October 2008 and February 2009. Characteristics of frequency, intensity and duration of vocalization were analyzed by GLM (general linear model) and Duncan's multi-test. There were differences in basic and maximum frequency, and intensity based on analysis of spectrogram and spectrum among different cage sizes. Vocalization of laying hen would be one of the indicators to understand the stress caused by rearing space in batter cage.