• Journal of Soil and Groundwater Environment
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• v.20 no.5
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• pp.47-51
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• 2015

Biocatalytic degradation of total petroleum hydrocarbons (TPHs) in contaminated soil by hemoglobin and hydrogen peroxide is an effective soil remediation method. This study used a laboratory soil reactor experiment to evaluate the effectiveness of a nonspecific biocatalytic reaction with hemoglobin and H₂O₂ for treating TPH-contaminated soil. We also quantified changes in the soil microbial community using real-time PCR analysis during the experimental treatment. The results show that the measured rate constant for the reaction with added hemoglobin was 0.051/day, about 3.5 times higher than the constant for the reaction with only H₂O₂ (0.014/day). After four weeks of treatment, 76% of the initial soil TPH concentration was removed with hemoglobin and hydrogen peroxide treatment. The removal of initial soil TPH concentration was 26% when only hydrogen peroxide was used. The soil microbial community, based on 16S rRNA gene copy number, was higher (7.1 × 10⁶ copy number/g of bacteria, and 7.4 × 10⁵ copy number/g of Archaea, respectively) in the hemoglobin catalyzed treatment. Our results show that TPH treatment in contaminated soil using hemoglobin catalyzed oxidation led to the enhanced removal effectiveness and was non-toxic to the native soil microbial community in the initial soil.

• Journal of Korean society of Dental Hygiene
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• v.20 no.1
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• pp.19-27
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• 2020

Objectives: This study aimed to analyze the association between self-assessed periodontal symptoms and glycated hemoglobin levels in patients with type 2 diabetes. Methods: This cross-sectional study involved 156 patients with type 2 diabetes who were aged 50 years or older. Structured questionnaires were used to investigate the self-assessed periodontal symptoms of the patients. The glycated hemoglobin test was performed to evaluate their long-term blood glycemic control. Chi-square test and logistic multiple regression were performed to analyze the factors associated with glycated hemoglobin levels. Results: Compared with patients aged 65 years and above, more patients aged 64 years and below showed poor glycemic control (p=0.020). Further, compared with patients without self-perceived gingival bleeding and halitosis, more patients with these two conditions showed poor glycemic control (p<0.05). Compared with the group of patients without any periodontal symptoms, the group of patients that had at least one periodontal symptom had a higher proportion of patients with poor glycemic control (p<0.001). In the logistic regression model, gingival bleeding and halitosis were the factors most associated with hyperglycemia (p<0.05). Conclusions: The results of our study suggest that gingival bleeding and halitosis can predict hyperglycemia in patients with type 2 diabetes.

• The Journal of Korean Medicine
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• v.23 no.1
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• pp.112-119
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• 2002

Objectives : Anemia is the center of interest in every maternity, both during pregnancy and lactation. The oriental postpartum care has one principle : to invigorate Qi and blood greatly. In this study, we seek to confirm that most sufferers of anemia recover under oriental postpartum care. To assure that this study had significance in non-iron supplement, we made a comparative study between oral iron supplement and non-iron supplement groups in oriental postpartum care. Methods : A comparative study was conducted to discern the difference in hemoglobin change between admission and after 2 weeks under the circumstances of 30 oral iron supplemented and 38 non-iron supplemented women who were admitted to the hospital attached to Woosuk University Oriental Medical College from April to August 2001 for postpartum care. Results : The mean hemoglobin level of after 2 weeks was 11.95 mg% (p<0.001). The mean hemoglobin level after 2 weeks was 11.65 mg% and 12.18 mg% in the iron supplemented and non-iron supplemented women respectively. The difference between the two groups was not significant. Conclusions : Oriental postpartum care overcomes anemia significantly in postpartum maternity. This is confirmed significantly under the non-iron supplement.

• Journal of Korean Biological Nursing Science
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• v.23 no.2
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• pp.119-126
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• 2021

Purpose: This study confirmed the effects of body mass index (BMI) and waist circumference (WC) on fasting blood glucose (FBS) and hemoglobin A1c (HbA1c) in adults without diabetes mellitus (DM). Methods: The subjects were 4,659 adults (≥ 20 years), and data were extracted from the 6th National Health and Nutrition Survey 2015 (KNHANES VI-3). The subject's data were analyzed using a complex sample t-test, χ²-test, and multiple logistic regression analysis (SPSS 24.0). Results: The incidence of the high FBS group was increased 1.60 times in males with a WC ≥ 90 cm and 1.78 times in females with a WC ≥ 85 cm. The incidence of the high HbA1c group was increased 1.54 times in those who were overweight and 2.22 times in those who were obese. The incidence of the high HbA1c group was increased 1.99 times in males with a WC ≥ 90 cm and 1.87 times in females with a WC ≥ 85 cm. Conclusion: This study presented evidence that interventions for BMI and WC should be included in DM prevention programs. It also suggested that these findings can be utilized for early detection of DM.

• Korean Journal of Psychosomatic Medicine
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• v.24 no.1
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• pp.94-101
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• 2016

Objectives : Anemia is a common problem in the management of elderly patients. Recent studies reported that anemia was associated with cognitive impairment and Alzheimer's disease. This study was aimed to analyze the differences of hemoglobin levels in Korean patients with Alzheimer's disease(AD) dementia, mild cognitive impairment(MCI), and healthy controls. Furthermore, the study also examined if any association between hemoglobin levels and cognitive functions existed. Methods : A total of 116 Korean elderly adults were participated in this study(mean age 74.67 years ; 60.3% female). The Korean version of the Mini-Mental State Examination(MMSE-K), Clinical Dementia Rating(CDR) and Global Deterioration Scale(GDS) were applied to all subjects. Hematological and related blood chemistry values were investigated. Results : We found that patients with AD dementia had significantly lower hemoglobin and hematocrit levels than MCI subjects and healthy control group. The hemoglobin levels showed a positive correlation with MMSE-K and negative correlation with CDR, GDS. Conclusions : Among Korean elderly, low hemoglobin level is associated with a cognitive impairment. This study indicates that AD is associated with anemia, and low hemoglobin levels may contribute to potentially useful clinical markers of AD. The risk factor for dementia needs to be confirmed by prospective longitudinal studies in a larger group of patients.

• BMB Reports
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• v.31 no.5
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• pp.503-507
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• 1998

To investigate the effects of the Vitreoscilla hemoglobin (VHb) gene on the production of a heterologous protein, a comparative expression system for VHb and ferritin was constructed. First, the VHb gene was inserted into the downstream and upstream regions of the ferritin gene to construct pHF2 and pHF3, respectively. Next, the two plasmids pACHB1 and pVUTFH10, having the VHb gene and the ferritin gene respectively, were constructed in order to express the two genes in different plasmids by using a coplasmid expression system. It was observed that the cell growth was improved in all strains containing the VHb gene. Furthermore, in our coplasmid expression system, the presence of the VHb gene increased production of the ferritin by 1.8 times, as much as that in a strain not having the VHb gene.

• Biomedical Science Letters
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• v.11 no.1
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• pp.85-88
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• 2005

Hemoglobin is oxygen carrier protein within erythrocyte in blood. Apoprotein of this, globin, is synthesized in the cytosol but it's cofactor, heme, is synthesized in the mitochondria. It has not been known very well how globin receives the heme from mitochondria and folds to hemoglobin. In this folding process, the initial structure of globin seems to be very important. A small volume of globin at acid pH was added rapidly into the bulk of an egg phosphatidylcholine $60\%$ liposome, containing hemins, at neutral pH according to the Rapid Dilution method. It was observed that an acid-induced unfolding structure of globin is initially needed to receive hemins from the lipid bilayer of liposomes. Also, this conclusion was confirmed with the absorption spectrum of the refolded globin separated by centrifugation.

• Bulletin of the Korean Chemical Society
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• v.31 no.7
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• pp.2103-2106
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• 2010

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.26-35
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• 2017

The hydrolysis of proteins constitutes an invaluable tool, granting access to a variety of peptide fragments with potentially interesting biological properties. Therefore, a hemoglobin (Hb) hydrolysate of Crocodylus siamensis was generated by digestion under acidic conditions. The antibacterial and antioxidant activities of the Hb hydrolysate were assessed in comparison with intact Hb. A disc diffusion assay revealed that the Hb hydrolysate exhibited antibacterial activity against eight strains of gram-positive bacteria and showed a higher efficacy than intact Hb. Moreover, the antioxidant activity of intact Hb and its hydrolysate was evaluated using ABTS and DPPH radical scavenging assays. The Hb hydrolysate exhibited free radical scavenging rates of 6-32%, whereas intact Hb showed a slightly higher activity. In addition, non-toxicity to human erythrocytes was observed after treatment with quantities of Hb hydrolysate up to $10{\mu}g$. Moreover, active fragmented Hb (P3) was obtained after purifying the Hb hydrolysate by reversed-phase HPLC. Scanning electron microscopy demonstrated the induction of bacterial cell membrane abnormalities after exposure to P3. Antibacterial and antioxidant activities play crucial roles for supporting the wound healing activity. Consequently, an in vivo mice excisional skin wound healing assay was carried out to investigate the effects of intact Hb treatment on wound healing in more detail. The results clearly demonstrate that intact Hb is capable of promoting 75% wound closure within 6 days. These findings imply that intact Hb of C. siamensis and its acid hydrolysate may serve as valuable precursors for food supplementary products benefitting human health.

• Journal of the Optical Society of Korea
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• v.16 no.1
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• pp.57-62
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• 2012

Using continuous wave near-infrared spectroscopy, we measured time-resolved concentration changes of oxy-hemoglobin and deoxy-hemoglobin from the primary motor cortex following finger tapping tasks. These data were processed using partial least squares-discriminant analysis (PLS-DA) to develop a prediction model for a brain-computer interface. The tasks were composed of a series of finger tapping for 15 sec and relaxation for 45 sec. The location of the motor cortex was confirmed by the anti-phasic behavior of the oxy- and deoxy-hemoglobin changes. The results were compared with those obtained using the hidden Markov model (HMM) which has been known to produce the best prediction model. Our data imply that PLS-DA makes better judgments in determining the onset of the events than HMM.