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http://dx.doi.org/10.7857/JSGE.2015.20.5.047

Enhanced Biodegradation of Total Petroleum Hydrocarbons (TPHs) in Contaminated Soil using Biocatalyst  

Owen, Jeffrey S. (Department of Environmental Science, Hankuk University of Foreign Studies)
Pyo, Sunyeon (Department of Environmental Science, Hankuk University of Foreign Studies)
Kang, Guyoung (Department of Environmental Science, Hankuk University of Foreign Studies)
Publication Information
Journal of Soil and Groundwater Environment / v.20, no.5, 2015 , pp. 47-51 More about this Journal
Abstract
Biocatalytic degradation of total petroleum hydrocarbons (TPHs) in contaminated soil by hemoglobin and hydrogen peroxide is an effective soil remediation method. This study used a laboratory soil reactor experiment to evaluate the effectiveness of a nonspecific biocatalytic reaction with hemoglobin and H2O2 for treating TPH-contaminated soil. We also quantified changes in the soil microbial community using real-time PCR analysis during the experimental treatment. The results show that the measured rate constant for the reaction with added hemoglobin was 0.051/day, about 3.5 times higher than the constant for the reaction with only H2O2 (0.014/day). After four weeks of treatment, 76% of the initial soil TPH concentration was removed with hemoglobin and hydrogen peroxide treatment. The removal of initial soil TPH concentration was 26% when only hydrogen peroxide was used. The soil microbial community, based on 16S rRNA gene copy number, was higher (7.1 × 106 copy number/g of bacteria, and 7.4 × 105 copy number/g of Archaea, respectively) in the hemoglobin catalyzed treatment. Our results show that TPH treatment in contaminated soil using hemoglobin catalyzed oxidation led to the enhanced removal effectiveness and was non-toxic to the native soil microbial community in the initial soil.
Keywords
Hemoglobin; Hydrogen peroxide; Soil remediation; Total petroleum hydrocarbon (TPH); Real time PCR;
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