• 제목/요약/키워드: hematological profile

검색결과 59건 처리시간 0.029초

Subacute Oral Toxicity and Bacterial Mutagenicity Study of a Mixture of Korean Red Ginseng (Panax ginseng C.A. Meyer) and Salvia plebeia R. Br. Extracts

  • Seo, Hwi Won;Suh, Jae Hyun;Kyung, Jong-Soo;Jang, Kyoung Hwa;So, Seung-Ho
    • Toxicological Research
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    • 제35권3호
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    • pp.215-224
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    • 2019
  • As various populations are rapidly becoming an aging society worldwide and interest in health issues has increased, demand for functional foods including herbal products has increased markedly to maintain a healthy state which has led to safety issues about their intake as an inevitable result. The objective of this study was to identify the safety profile of a Korean red ginseng and Salvia plebeia R. Br. extract mixture (KGC-03-PS) which is a valuable ingredient that can be used as a functional food. In the present study, the subacute oral toxicity and bacterial reverse mutagenicity of KGC-03-PS were evaluated. Sprague Dawley rats were administered KGC-03-PS orally for 28 days by gavage. Daily KGC-03-PS dose concentrations were 0, 500, 1,000, or 2,000 mg/kg body weight (bw) per day. Bacterial reverse mutation test with KGC-03-PS dose levels ranging from 312.5 to $5,000{\mu}g/plate$ was carried out by OECD test guideline No. 471. Five bacterial strains (Salmonella typhimurium TA98, TA100, TA1535, TA1537, and Escherichia coli WP2) were tested in the presence or absence of metabolic activation by plate incorporation method. There were no toxicological effects related with test substance in the clinical evaluation of subacute oral toxicity test including clinical signs, body weight, and food consumption. Moreover, no toxicological changes related to KGC-03-PS were observed in the hematological and serum biochemical characteristics as well as in the pathological examinations, which included organ weight measurements and in the gross- or histopathological findings. KGC-03-PS did not induce an increase in the number of revertant colonies in all bacterial strains of the bacterial reverse mutation test. The no-observed-adverse-effect level of KGC-03-PS is greater than 2,000 mg/kg bw/day, and KGC-03-PS did not induce genotoxicity related to bacterial reverse mutations under the conditions used in this study.

Potential Probiotic Characteristics and Safety Assessment of Lactobacillus rhamnosus SKG34 Isolated from Sumbawa Mare's Milk

  • Sujaya, I Nengah;Suwardana, Gede Ngurah Rsi;Gotoh, Kazuyoshi;Sumardika, I Wayan;Nocianitri, Komang Ayu;Sriwidyani, Ni Putu;Putra, I Wayan Gede Artawan Eka;Sakaguchi, Masakiyo;Fatmawati, Ni Nengah Dwi
    • 한국미생물·생명공학회지
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    • 제50권1호
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    • pp.51-62
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    • 2022
  • Lactobacillus rhamnosus SKG34 (LrSKG34), a potential probiotic strain, was successfully isolated from Sumbawa Mare's milk. Our previous studies showed that the strain is resistant to gastrointestinal conditions, possesses antioxidant activity, and lowers blood cholesterol levels. Further clarification of the potential probiotic characteristics and safety assessment are necessary. This study aimed to evaluate the adhesion of LrSKG34 to Caco-2 cell monolayers and its effect on mucosal integrity in vitro. We also examined the LrSKG34 safety profile based on antimicrobial susceptibility testing, haemolytic activity determination, Caco-2 cell monolayer translocation evaluation, and in vivo investigation of the effect of LrSKG34 on the physiology, biochemical markers, and histopathological appearance of major organs in an animal model. LrSKG34 attached to Caco-2 cell monolayers and maintained mucosal integrity in vitro. The typical resistance of lactobacilli to ciprofloxacin, gentamicin, vancomycin, trimethoprim-sulfamethoxazole, and metronidazole was confirmed for LrSKG34. No haemolytic activity was observed on blood agar plates, and no LrSKG34 translocation was observed in Caco-2 cell monolayers. Administration of LrSKG34 to Sprague-Dawley rats did not adversely affect body weight. No abnormalities in hematological parameters, serum biochemistry levels, or histopathological structures of major organs were observed in LrSKG34-treated rats. Collectively, the results implicate LrSKG34 as a promising and potentially safe probiotic candidate for further development.

Ustekinumab pharmacokinetics after subcutaneous administration in swine model

  • Grabowski, Tomasz;Burmanczuk, Artur;Derlacz, Rafal;Stefaniak, Tadeusz;Rzasa, Anna;Borkowski, Jacek
    • Journal of Veterinary Science
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    • 제22권5호
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    • pp.47.1-47.10
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    • 2021
  • Background: Due to multiple similarities in the structure and physiology of human and pig skin, the pig model is extremely useful for biological drug testing after subcutaneous administration. Knowledge of the differences between subcutaneous injection sites could have a significant impact on the absorption phase and pharmacokinetic profiles of biological drugs. Objectives: This study aimed to analyze the impact of administration site on pharmacokinetics and selected biochemical and hematological parameters after a single subcutaneous administration of ustekinumab in pigs. Drug concentrations in blood plasma were analyzed by enzyme-linked immunosorbent assay. Pharmacokinetic analyses were performed based on raw data using Phoenix WinNonlin 8.1 software and ThothPro v 4.1. Methods: The study included 12 healthy, female, large white piglets. Each group received a single dose of ustekinumab given as a 1 mg/kg subcutaneous injection into the internal part of the inguinal fold or the external part of the inguinal fold. Results: The differences in absorption rate between the internal and external parts of the inguinal fold were not significant. However, the time of maximal concentration, clearance, area under the curve calculated between zero and mean residence time and mean residence time between groups were substantially different (p > 0.05). The relative bioavailability after administration of ustekinumab into the external part of the inguinal fold was 40.36% lower than after administration of ustekinumab into the internal part of the inguinal fold. Conclusions: Healthy breeding pigs are a relevant model to study the pharmacokinetic profile of subcutaneously administered ustekinumab.

Effects on hematology and blood biochemistry profile of intramuscular meloxicam injection in Brahminy kite and Barn owl

  • Ratiwan Sitdhibutr;Raveewan Ploypan;Sirawit Subaneg;Chaiyan Kasorndorkbua
    • Journal of Veterinary Science
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    • 제24권3호
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    • pp.43.1-43.8
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    • 2023
  • Background: Meloxicam is used widely for exotic animal analgesia, but its toxicity in common raptor species in Thailand is unclear. Objectives: This study evaluated the single-dose effect of intramuscular meloxicam in common raptor species in Thailand for short-term and long-term periods. Methods: Twenty-two raptors were administered a single 1 mg/kg dose of meloxicam individually via intramuscular injection. The following were evaluated: clinical appearance, body weight, body condition score, body temperature, fecal appearance, complete blood cell count, and biochemistry panel before (day 0) and after the injection (1, 7, and 30 days). The collected samples were categorized into three groups: Brahminy kite (Haliastur indus) (n = 10), adult Barn owl (Tyto javanica) (n = 4), and juvenile Barn owl (n = 8). Results: None of the raptors in the study groups showed any abnormalities. The hematological profiles were significantly different in the short-term period (day 1 and day 7). The creatinine, aspartate aminotransferase, and creatinine kinase increased in several groups. On the other hand, the packed cell volume decreased in the Brahminy kite and juvenile Barn owl groups. According to the findings, an intramuscular injection of 1 mg/kg meloxicam affected the blood biochemistry panel of the muscle, but the affected raptors recovered within one week. Conclusions: An intramuscular injection of meloxicam at a single 1 mg/kg dose in Brahminy kites and Barn owls was not associated with the morbidity, hepatotoxicity, gastrointestinal toxicity, and nephrotoxicity in the short- and long-term periods.

Latitude and seasons influence the prevalence of Theileria orientalis and affect the hematology of non-grazed dairy cows in Korea

  • Hector Espiritu;Hee-Woon Lee;Md Shohel Al Faruk;Su-Jeong Jin;Sang-Suk Lee;Yong-Il Cho
    • Parasites, Hosts and Diseases
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    • 제62권1호
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    • pp.64-74
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    • 2024
  • This pilot study aimed to investigate the effects of regional and seasonal variations on the prevalence of Theileria orientalis and the hematological profile of non-grazed dairy cows in Korea. A total of 365 clinically healthy lactating Holstein Friesian cows from 26 dairy farms in 7 provinces that were categorized into northern, central, and southern regions were sampled during the warm period from July to August and the cold period from October to December. The detection of T. orientalis major piroplasm surface protein gene and the hematology non-grazed dairy cows were analyzed using peripheral blood samples. The T. orientalis prevalence was 20.0% (73/365). The prevalence in the southern region was 35.9%, which was significantly higher than that in the central (21.6%) and northern (12.9%) regions (P<0.05). The prevalence during warm period was higher (43.0%) than that during the cold season (13.5%). The infected cows showed significantly lower erythrocyte counts in the southern region (5.8±0.6 M/㎕) and during the warm period (5.8±0.7 M/㎕) compared with those in the central and northern regions and during the cold season, which affected the extended RBC parameters, including hematocrit and hemoglobin concentrations. Our findings revealed the prevalence of T. orientalis in Korea, highlighting its high occurrence during warm periods and in certain geographical regions. Climatic factors could contribute to the health and productivity of cattle, as evidenced by the prevalence of T. orientalis and its negative impact on animals.

오소리 지질이 인슐린 비의존형 당뇨환자의 건강상태 당뇨지표 및 혈청지질농도에 미치는 영향 (The Influences of Meles meles Oil on Health Status, Diabetic Index and Serum Lipid Profile in Non - Insulin Dependent Diabetes Mellitus Patients)

  • 박성혜;백승화;한종현
    • 한국식품영양과학회지
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    • 제33권7호
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    • pp.1139-1146
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    • 2004
  • 본 연구자들은 다른 동물성 지질에 비해 콜레스테롤 함량이 낮고 불포화지방산 함량이 높은 오소리 지질을 당뇨환자들에게 섭취시킨 후 혈당, 혈청의 당뇨지표 및 혈청 지질의 변화를 관찰하여 당뇨환자들에게 동물성인 오소리 지질 섭취가 혈당유지 및 합병증 관리 에 합당한가를 평가하여 오소리 지질을 기능성 식품의 원료로 활용할 수 있는가를 확인하고자 하였다. 일일 평균 7.26 g의 오소리 지질을 12주간 섭취한 후에 총 지질의 섭취량은 유의적 인 차이가 없었으나 $\omega$3계 지방산과 다가불포화지방산의 섭취량은 유의적으로 증가하였고 콜레스테롤의 섭취량은 유의적으로 감소된 결과를 나타냈다. 공복 시 혈당이 오소리 지질의 섭취에 따라 유의적인 상승을 나타내지 않았고 fructosamine과 glucagon농도는 오소리 지질의 섭취 6주째부터 유의적으로 상승되었다. 혈청의 LDL-콜레스테롤 및 중성지방의 농도는 오소리 지질 섭취에 따라 유의적으로 감소되었고 HDL-콜레스테롤 농도는 유의적으로 상승되었다. 또한 일반적인 건강상태 판정지표로 살펴볼 때 간기능 및 철분 영양상태가 증진되었다. 위의 결과들로 보아 제2형 당뇨환자의 12주간 오소리 지질의 섭취는 건강상태와 혈청지질 profile에는 유익한 영향을 주었고 따라서 동물성인 오소리 지질을 건강보조식품으로 사용하거나 기능성 식품의 원재료로 활용이 가능할 것으로 판단된다. 앞으로 오소리 지질 섭취 수준을 달리한 dose-response 실험을 통해 섭취 수준과 혈청지질 및 지방산 조성의 변화를 관찰하여 오소리 지질의 광범위한 활용을 위한 체계적인 자료를 더 확보하고자 한다.

Hemato-biochemical and Cortisol Profile of Holstein Growing-calves Supplemented with Vitamin C during Summer Season

  • Kim, Jong-Hyeong;Mamuad, Lovelia L.;Yang, Chul-Ju;Kim, Seon-Ho;Ha, Jong-K.;Lee, Wang-Shik;Cho, Kwang-Keun;Lee, Sang-Suk
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권3호
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    • pp.361-368
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    • 2012
  • Effect of vitamin C (VC) on biochemical, hematological and cortisol profile of growing Holstein calves during summer was investigated. Eighteen calves between 14 and 16 weeks of age were divided equally into two groups. One group received a diet supplemented with VC (20 g/d) for 60 days, while other non-supplemented diet fed group served as a control (CON). The temperature humidity index (THI) was recorded and computed during the experiment. From days 0 to 60, the THI exceeded 70. Blood samples were collected from the jugular vein of each calf at days 0, 15, 30, 45 and 60. Serum albumin and total protein decreased (p<0.05) in CON and VC calves with age. Serum glutamic-oxaloacetic transaminase concentrations were not affected by treatments. Serum creatinine, albumin and glutamic-pyruvic transaminase concentrations were higher in calves in the VC group than the CON group. While red blood cells, hemoglobin and hematocrit were lower (p<0.05) in VC calves, mean corpuscular volume, mean corpuscular hemoglobin, red blood cell distribution width and mean platelet volume were higher (p<0.05) in these VC supplemented calves. Leukocyte parameters including white blood cells and full term for lymphocytes were not affected by the treatments. Also, serum cortisol was not affected by treatments. At day 15, 30 and 45, the total VC in plasma was higher (p<0.05) in calves fed with VC. In conclusion, serum cortisols were not affected by plasma VC concentration, while some blood parameters were positively influenced in calves fed with VC.

성인 남자의 흡연상태에 따른 영양섭취, 혈압, 혈액 성상 및 지질패턴 비교연구 (Comparative Study on Nutrient Intakes, Blood Pressure and Serum Lipid Profile of Korean Adult Men According to Smoking Status)

  • 최미경;조혜경;승정자
    • 한국식품영양과학회지
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    • 제35권2호
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    • pp.164-170
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    • 2006
  • 본 연구에서는 흡연율이 높은 성인 남자에 있어 흡연상태에 따라 비흡연군(47명), 흡연군(58명), 흡연경험군(44명)으로 나눠 영양섭취와 혈압, 혈액 성상 및 지질패턴을 비교분석하였다. 전체대상자의 평균 연령, 신장, 체중, 체질량지수 각각 54.7세, 165.8 cm, 67.3 kg, $24.5\;kg/m^2$이었으며, 흡연 상태에 따른 세 군간 유의한 차이가 없었다. 1일 평균 에너지와 단백질 섭취량은 각각 1740.9 kcal와 59.69 g이었으며, 흡연군간 유의한 차이가 없었다. 엽산 섭취량은 비흡연군 $324.2{\mu}g$, 흡연군 $235.1{\mu}g$, 흡연경험군 $272.7{\mu}g$으로 흡연군이 유의하게 낮았으나, 콜레스테롤 섭취량은 비흡연군 175.6mg, 흡연군 232.5 mg, 흡연경험군 154.3 mg으로 흡연군이 유의하게 높았다. 1일 총 식품 섭취량은 비흡연군 1250.0 g, 흡연군 1180.1 g, 흡연경험군 1237.5 g으로 세 군간 유의한 차이가 없었다. 버섯류의 섭취량은 비흡연군 1.5g, 흡연군 0.4g, 흡연경험군 4.8 g으로 흡연군이 유의하게 낮은 반면, 난류의 섭취량은 세 군 각각 8.3 g, 17.8 g, 4.4 g으로 흡연군이 유의하게 높았다. 전체대상자의 적혈구수와 혈청 GOT, GPT는 각각 $4598.2\times1000/mm^2$, 27.3 U/L, 27.3 U/L이었으며 비흡연군, 흡연군, 흡연경험군간 유의한 차이가 없었다. 그러나 백혈구수, 헤마토크릿, 헤모글로빈 농도는 흡연군이 각각 $6727.7/mm^3,\;45.9\%$, 14.7g/dL로 비흡연군($4921.5/mm^3,\;43.4\%$, 13.9 g/dL)이나 흡연경험군($5845.4/mm^3,\;44.2\%$, 13.9 g/dL)보다 유의하게 높았다. 전체대상자의 평균 혈압은 128.3/75.5 mmHg이었으며, 혈청 콜레스테롤, 중성지방, HDL-콜레스테롤, LDL-콜레스테롤, 동맥경화지수는 각각 180.2 mg/dL, 160.8 mg/dL, 41.5 mg/dL, 106.5 mg/dL, 3.5로 흡연상태에 따른 세 군간 유의한 차이가 없었다. 그러나 흡연군의 중성지질 농도는 비흡연군보다 유의하게 높았다. 이상의 연구결과를 종합할 때 흡연자는 비흡연자보다 엽산 섭취가 낮고 콜레스테롤 섭취가 높았으며, 백혈구수, 헤마토크릿과 헤모글로빈, 중성지질 함량이 높게 나타나 흡연이 일부 영양소의 섭취와 혈액성상에 변화를 초래하는 것으로 보여진다. 또한 흡연경험자는 비흡연자와 영양소 섭취와 혈액성상에 유의한 차이를 보이지 않아 흡연으로 인한 일부 영양소 섭취와 혈액성상의 변화는 금연으로 인해 비흡연 상태로 회복되는 것으로 생각된다. 따라서 흡연자를 대상으로 한 금연 교육이 우선적으로 요구되며, 흡연으로 인한 영양섭취의 변화가 인체에 미치는 영향을 설명 할 수 있는 직접적이고 세부적인 연구를 통해 흡연자의 영양 문제점과 그에 따른 식사관리 방안이 마련되어야 할 것이다.

Effects of Enzyme Addition to Broiler Diets Containing Varying Levels of Double Zero Rapeseed Meal

  • Ramesh, K.R.;Devegowda, G.;Khosravinia, H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권9호
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    • pp.1354-1360
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    • 2006
  • Maize-soybean meal diets with 0, 100, 200 and 300 g/kg double zero rapeseed meal ('00' RSM) with and without an enzyme mixture (xylanase, pectinase, cellulase) at a level of 1.6 g/kg were evaluated with 624 day-old broiler chicks for 5 weeks. The birds were randomly allocated to eight dietary treatments with three replicates of 26 birds each. Average daily gain (ADG) and feed intake (FI) were recorded weekly and ileal viscosity, organ weights, serum enzyme activity, hormonal profile and hematological parameters were measured at the end of week 5. Average daily gain during the weekly periods was significantly influenced by the dietary level of '00'RSM (p<0.01). Inclusion of '00' RSM improved the ADG up to day 28 with the increased level; beyond that time no improvement was recorded when compared to control groups. However, ADG from 1-35 days was significantly different between 300 g/kg inclusion level of '00' RSM and the control diet. Inconsistent decline in feed intake and feed conversion ratio was observed up to day 21 and the trend was reversed thereafter. The proportion of '00' RSM in the diet had a significant ($p{\leq}0.05$) influence on thyroid weight but had no effect on the relative weights of liver and heart, serum enzyme activities (${\gamma}$-glutamyl transferase, alanine amino transferase and aspartate amino transferase), thyroid hormones ($T_3$ and $T_4$), hemoglobin level and hematocrit. Significant improvement in ADG was recorded during the 2nd week of age with the addition of enzyme, whereas for all other periods, including the whole period of the trial, higher but non-significant ADG was observed. FI and FCR were not affected by the addition of enzyme but there was a numerical reduction in FCR during the whole period. The addition of enzyme reduced the ileal viscosity at all levels of '00' RSM inclusion. The results suggest that '00' RSM can be included up to 300 g/kg in broiler diets without any adverse effects on health and performance. The addition of commercial enzyme mixture containing xylanase, pectinase, cellulase to broiler diets containing '00'RSM has some effect on growth rate and feed conversion efficiency.

Silencing of Suppressor of Cytokine Signaling-3 due to Methylation Results in Phosphorylation of STAT3 in Imatinib Resistant BCR-ABL Positive Chronic Myeloid Leukemia Cells

  • Al-Jamal, Hamid AN;Jusoh, Siti Asmaa Mat;Yong, Ang Cheng;Asan, Jamaruddin Mat;Hassan, Rosline;Johan, Muhammad Farid
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권11호
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    • pp.4555-4561
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    • 2014
  • Background: Silencing due to methylation of suppressor of cytokine signaling-3 (SOCS-3), a negative regulator gene for the JAK/STAT signaling pathway has been reported to play important roles in leukemogenesis. Imatinib mesylate is a tyrosine kinase inhibitor that specifically targets the BCR-ABL protein and induces hematological remission in patients with chronic myeloid leukemia (CML). Unfortunately, the majority of CML patients treated with imatinib develop resistance under prolonged therapy. We here investigated the methylation profile of SOCS-3 gene and its downstream effects in a BCR-ABL positive CML cells resistant to imatinib. Materials and Methods: BCR-ABL positive CML cells resistant to imatinib (K562-R) were developed by overexposure of K562 cell lines to the drug. Cytotoxicity was determined by MTS assays and $IC_{50}$ values calculated. Apoptosis assays were performed using annexin V-FITC binding assays and analyzed by flow cytometry. Methylation profiles were investigated using methylation specific PCR and sequencing analysis of SOCS-1 and SOCS-3 genes. Gene expression was assessed by quantitative real-time PCR, and protein expression and phosphorylation of STAT1, 2 and 3 were examined by Western blotting. Results: The $IC_{50}$ for imatinib on K562 was 362nM compared to 3,952nM for K562-R (p=0.001). Percentage of apoptotic cells in K562 increased upto 50% by increasing the concentration of imatinib, in contrast to only 20% in K562-R (p<0.001). A change from non-methylation of the SOCS-3 gene in K562 to complete methylation in K562-R was observed. Gene expression revealed down-regulation of both SOCS-1 and SOCS-3 genes in resistant cells. STAT3 was phosphorylated in K562-R but not K562. Conclusions: Development of cells resistant to imatinib is feasible by overexposure of the drug to the cells. Activation of STAT3 protein leads to uncontrolled cell proliferation in imatinib resistant BCR-ABL due to DNA methylation of the SOCS-3 gene. Thus SOCS-3 provides a suitable candidate for mechanisms underlying the development of imatinib resistant in CML patients.