• 제목/요약/키워드: helicase

검색결과 105건 처리시간 0.03초

인진청간탕(茵蔯淸肝湯)의 B형(型) 및 C형(型) 간염 바이러스에 대한 항(抗) 바이러스 효과(效果) (The inhibition effects of Injinchunggantang on Hepatitis B and C Virus)

  • 김수성;김기열;윤철호;서운교;김종대;정지천
    • 동국한의학연구소논문집
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    • 제8권1호
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    • pp.93-106
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    • 1999

  • 우리나라와 같이 B형 간염 바이러스의 유병율이 높은 지역에서는 B형 간염 바이러스뿐만 아니라 HCV에 대한 항 바이러스 효과도 동시에 시행함이 유용할 것으로 사료되었다. 본 연구에서는 인진청간탕(菌蔯淸肝湯)을 이용하여 B형 간염 바이러스에 대한 항(抗) 바이러스 효과를 PCR을 이용하여 HBV의 DNA를 검색하였다. 인진청간탕(菌蔯淸肝湯)이 처리된 처리군의 경우 대조군에 비하여 농도 의존적으로 B형 간염 바이러스의 표면항원 생산을 억제하였고, PCR을 이용하여 증폭한 경우에도 증폭된 DNA의 양은 감소하였다. 또한 C형 간염 바이러스에 대한 항(抗) 바이러스 효과를 검색하기 위하여 복제에 필수적인 단백질인 HCV helicase의 ATPase에 대한 활성 억제 효과를 조사하였다. 인진청간탕(菌蔯淸肝湯)이 HCV helicase의 ATPase 활성을 억제하는 효과가 있음을 확인한 후 dose-respond에 따른 실험을 수행하여 HCV helicase의 ATPase 활성을 억제함으로써 탈인산화 되지 않은 ATP의 양을 이용하여 PSL로 분석한 결과 인진청간탕(菌蔯淸肝湯)이 HCV helicase의 ATPase 기능에 대한 억제효과가 우수함이 확인되었다. 이상의 결과로 보아 인진청간탕(菌蔯淸肝湯)이 B형 및 C형 간염치료에 동시에 응용될 수 있을 것으로 사료된다.

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Isolation of HRD3 gene, a homologous RAD3 gene from fission yeast Schizosaccharomyces pombe

  • Choi, In-Soon;Jin, Yong-Hwan;Park, Sang-Dai
    • 한국환경성돌연변이발암원학회지
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    • 제16권2호
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    • pp.77-82
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    • 1996

  • The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA-RNA helicase activies. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, we have cloned the RAD3 homolog, HRD3, from the distantly related yeast Schizosaccharomyces pombe. Here, we report the partial cloning and characterization of HRD3 gene (Homologous of RAD3 gene) which was isolated by PCR amplification using conserved domain of Saccharomyces cerevisiae RAD3 gene. Chromosomal DNA isolated from S. pombe had similar restriction patterns to those from S. cerevisiae, as determined by Southern blot analysis. The 2. 8 kb transcript of mRNA was identified by Northern hybridization. The level of transcript did not increase upon UV-irradiation, suggesting that the HRD3 gene in S. pombe is not UV-inducible.

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Analysis of In Vivo Interaction of HCV NS3 Protein and Specific RNA Aptamer with Yeast Three-Hybrid System

  • HWANG BYOUNGHOON;LEE SEONG-WOOK
    • Journal of Microbiology and Biotechnology
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    • 제15권3호
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    • pp.660-664
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    • 2005

  • We have previously isolated specific RNA aptamers with high affinity against the helicase domain of hepatitis C virus (HCV) nonstructural protein 3 (NS3). The RNA aptamers competitively and efficiently inhibited the helicase activity, partially impeding HCV replicon replication in human hepatocarcinoma cells. In this study, the RNA aptamers were tested for binding to the HCV NS3 proteins in eukaryotic cells, using a yeast three-hybrid system. The aptamers were then recognized by the HCV NS3 proteins when expressed in the cells, while the antisense sequences of the aptamers were not. These results suggest that the in vitro selected RNA aptamers can also specifically bind to the target proteins in vivo. Consequently, they could be potentially utilized as anti-HCV lead compounds.

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Expression of the mexA Gene Requires the DNA Helicase RecG in Pseudomonas aeruginosa PAO1

  • Heo, Aram;Park, Woojun
    • Journal of Microbiology and Biotechnology
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    • 제25권4호
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    • pp.492-495
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    • 2015

  • This study provides evidence that RecG regulates the expression of the OxyR-independent gene mexA in Pseudomonas aeruginosa PAO1. A reduction in mexA expression was observed in the absence of RecG, but not OxyR, by northern blot and quantitative real-time PCR analyses. The canonical palindromic RecG binding sequence was present upstream of the mexA promoter, and bound purified RecG and single strand-binding protein. These data reveal a novel mechanism of OxyR-independent gene transcription by RecG.

  • https://doi.org/10.4014/jmb.1410.10011 인용 PDF KSCI KPUBS

The translational landscape as regulated by the RNA helicase DDX3

  • Park, Joon Tae;Oh, Sekyung
    • BMB Reports
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    • 제55권3호
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    • pp.125-135
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    • 2022

  • Continuously renewing the proteome, translation is exquisitely controlled by a number of dedicated factors that interact with the ribosome. The RNA helicase DDX3 belonging to the DEAD box family has emerged as one of the critical regulators of translation, the failure of which is frequently observed in a wide range of proliferative, degenerative, and infectious diseases in humans. DDX3 unwinds double-stranded RNA molecules with coupled ATP hydrolysis and thereby remodels complex RNA structures present in various protein-coding and noncoding RNAs. By interacting with specific features on messenger RNAs (mRNAs) and 18S ribosomal RNA (rRNA), DDX3 facilitates translation, while repressing it under certain conditions. We review recent findings underlying these properties of DDX3 in diverse modes of translation, such as cap-dependent and cap-independent translation initiation, usage of upstream open reading frames, and stress-induced ribonucleoprotein granule formation. We further discuss how disease-associated DDX3 variants alter the translation landscape in the cell.

  • https://doi.org/10.5483/BMBRep.2022.55.3.188 인용 PDF KSCI