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http://dx.doi.org/10.4014/jmb.1410.10011

Expression of the mexA Gene Requires the DNA Helicase RecG in Pseudomonas aeruginosa PAO1  

Heo, Aram (Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University)
Park, Woojun (Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.4, 2015 , pp. 492-495 More about this Journal
Abstract
This study provides evidence that RecG regulates the expression of the OxyR-independent gene mexA in Pseudomonas aeruginosa PAO1. A reduction in mexA expression was observed in the absence of RecG, but not OxyR, by northern blot and quantitative real-time PCR analyses. The canonical palindromic RecG binding sequence was present upstream of the mexA promoter, and bound purified RecG and single strand-binding protein. These data reveal a novel mechanism of OxyR-independent gene transcription by RecG.
Keywords
DNA helicase RecG; OxyR; Pseudomonas aerruginosa; efflux pump; mexA; transcriptional regulator;
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1 Ochsner UA, Vasil ML, Alsabbagh E, Parvatiyar K, Hassett DJ. 2000. Role of the Pseudomonas aeruginosa oxyR-recG operon in oxidative stress defense and DNA repair: OxyR-dependent regulation of katB-ankB, ahpB, and ahpC-ahpF. J. Bacteriol. 182: 4533-4544.   DOI
2 Ochsner UA, Hassett DJ, Vasil ML. 2001. Genetic and physiological characterization of ohr, encoding a protein involved in organic hydroperoxide resistance in Pseudomonas aeruginosa. J. Bacteriol. 183: 773-778.   DOI   ScienceOn
3 Poole K, Srikumar R. 2001. Multidrug efflux in Pseudomonas aeruginosa: components, mechanisms and clinical significance. Curr. Top. Med. Chem. 1: 59-71.   DOI
4 Sánchez P, Rojo F, Martínez JL. 2002. Transcriptional regulation of mexR, the repressor of Pseudomonas aeruginosa mexAB-oprM multidrug efflux pump. FEMS Microbiol. Lett. 207: 63-68.   DOI
5 Sharples GJ, Ingleston SM, Lloyd RG. 1999. Holliday junction processing in bacteria: insights from the evolutionary conservation of RuvABC, RecG, and RusA. J. Bacteriol. 181: 5543-5550.
6 Yeom J, Lee Y, Park W. 2012. ATP-dependent RecG helicase is required for the transcriptional regulator OxyR function in Pseudomonas species. J. Biol. Chem. 287: 24492-24504.   DOI   ScienceOn
7 Cañas C, Suzuki Y, Marchisone C, Carrasco B, Freire-Benéitez V, Takeyasu K, et al. 2014. Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution. J. Biol. Chem. 289: 17634-17646.   DOI   ScienceOn
8 Daigle DM, Cao L, Fraud S, Wilke MS, Pacey A, Klinoski R, et al. 2007. Protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa. J. Bacteriol. 189: 5441-5451.   DOI   ScienceOn
9 Evans K, Adewoye L, Poole K. 2001. MexR repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa: identification of MexR binding sites in the mexA-mexR intergenic region. J. Bacteriol. 183: 807-812.   DOI   ScienceOn
10 Li XZ, Nikaido H, Poole K. 1995. Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob. Agents. Chemother. 39: 1948-1953.   DOI   ScienceOn
11 Lloyd RG, Buckman C. 1991. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair. J. Bacteriol. 173: 1004-1011.   DOI
12 Maseda H, Yoneyama H, Nakae T. 2000. Assignment of the substrate-selective subunits of the MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 44: 658-664.   DOI
13 McGlynn P, Mahdi AA, Lloyd RG. 2000. Characterisation of the catalytically active form of RecG helicase. Nucleic Acids Res. 28: 2324-2332.   DOI
14 Morita Y, Cao L, Gould G, Avison MB, Poole K. 2006. nalD encodes a second repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa. J. Bacteriol. 188: 8649-8654.   DOI   ScienceOn
15 Buss JA, Kimura Y, Bianco PR. 2008. RecG interacts directly with SSB: implications for stalled replication fork regression. Nucleic Acids Res. 36: 7029-7042.   DOI   ScienceOn
16 Buyck JM, Guénard S, Plésiat P, Tulkens PM, Van Bambeke F. 2012. Role of MexAB-OprM in intrinsic resistance of Pseudomonas aeruginosa to temocillin and impact on the susceptibility of strains isolated from patients suffering from cystic fibrosis. J. Antimicrob. Chemother. 67: 771-775.   DOI   ScienceOn