• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.570-583
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• 2021

Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.

• 한국식품저장유통학회지
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• 제24권6호
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• pp.820-826
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• 2017

대두는 된장, 간장, 스낵 등 전통식품의 주요 원료이며, 열처리 공정은 대두의 가공과정에서 대부분 수반되는 단위 조작이다. 본 연구에서는 열처리가 대두의 이화학적 특성에 미치는 영향을 조사하기 위하여 상업적인 조건에서 대두를 증자, 가열팽화, 튀김 처리한 다음 단백질의 분자량 분포와 용해도, 수분과 유지 흡착력 및 열 특성과 산패도의 변화를 조사하였다. 대두는 가열처리에 의하여 단백질이 10-40 kDa 범위의 작은 분자량으로 분해되는 경향을 나타내었다. 대두의 용융 엔탈피는 199.62 J/g이었으며 열처리에 의하여 123.07-135.90 J/g 범위로 엔탈피가 감소하였고 지질 산화를 보여주는 fluorescence intensity도 열처리로 증가하였으며 열처리 효과는 튀김, 증자, 가열팽화의 순으로 높았다. 또한 대두의 수분 흡착력은 열처리 한 경우가 비열처리 대두보다 상대적으로 높았으며, 단백질의 용해도는 산성 영역(pH 3-6)에서 같은 경향을 보였다. 결과적으로 대두는 가열처리에 의하여 용융 엔탈피가 감소하고 단백질이 분해되며 지질 산화와 용해도 및 수분흡착력이 증가하는데 대두에 대한 열처리의 효과는 튀김 공정에서 뚜렷하였다.

• 열처리공학회지
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• 제26권4호
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• pp.185-190
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• 2013

Influence of solution treatment (T4) and peak-aging (T6) on damping capacity was investigated in permanent-mold cast Mg-3%Nd alloy. In as-cast state, the microstructure was characterized by eutectic $Mg_{12}Nd$ intermetallic phase network in the intergranular region. T4 treatment resulted in a dissolution of the eutectic particles, but small amount of the particles still remained in the microstructure. After T6 treatment, nano-sized ${\beta}^{\prime}(Mg_{12}Nd)$ particles were precipitated within the matrix. T4 microstructure showed higher damping capacity than as-cast and T6 ones. In view of the microstructural features, this may well be associated with the dissolution of second-phase particles which play a role in pinning the dislocations acting as a damping source.

• 수산해양기술연구
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• 제59권2호
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• pp.155-163
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• 2023

This study deals with the maximum thermal load analysis and optimal capacity determination method of tank culture system for applying seawater source heat pump to save energy and realize zero energy. The location of the fish farm was divided into four sea areas, and the heat load in summer and winter was analyzed, respectively. In addition, two representative methods, the flow-through aquaculture system and the recirculation aquaculture system were reviewed as water treatment methods for fish farms. In addition, the concept of the exchange rate was introduced to obtain the maximum heat load of the fish farms. Finally, power consumption for heat pumps was analyzed in the view point of sea areas, tank capacity, and exchange rate based on the calculated maximum thermal load.

• Metals and materials international
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• 제24권6호
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• pp.1346-1358
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• 2018

A novel additive manufacturing method with TIG-MIG hybrid heat source was applied for fabricating 5356 aluminum alloy component. In this paper the microstructure evolution, mechanical properties and fracture morphologies of both as-deposited and heat-treated component were investigated, and how these were affected by different heat-treated temperature. The as-deposited microstructure showed dominant equiaxed grains with second phase, and the size of them is coarse in the bottom region, medium in the middle region and fine in the top region owing to different thermal cycling conditions. Compared with as-deposited microstructure, the size of grain becomes large and second phases gradually dissolve in the matrix as heat-treated temperature increase. Different microstructures determine the mechanical properties of component. Results show that average ultimate tensile strength enhances from 226 to 270 MPa and average microhardness increases from 64.2 to 75.3 HV0.1 but ductility decreases from 33 to 6.5% with heat-treated temperature increasing. For all components, the tensile properties are almost the same in the vertical direction (Z) and horizontal direction (Y) due to equiaxed grains, which exhibits isotropy, and the mechanisms of these are analyzed in detailed. In general, the results demonstrate that hybrid arc heat source has the potential to fabricate aluminum alloy component.

• 열처리공학회지
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• 제36권2호
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• pp.86-95
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• 2023

This paper explained the equipment and process development to secure the source technology of controlled nitrification technology. The nitriding potential in the furnace was controlled only by adjusting the flow rate of ammonia gas introduced into the furnace. In addition, a control system was introduced to automate the nitriding process. The equipment's hardware was designed to enable controlled nitriding based on the conventional gas nitriding furnace, and an automation device was attached. As a result of measuring the temperature and quality uniformity for the equipment, the temperature and compound uniformity were ±1.2℃ and 14.3 ± 0.2 ㎛, respectively. And, it was confirmed that nitriding potential was controlled within the tolerance range of AMS2759-10B standard. In addition to parts for controlled nitriding, it was applied to products produced in existing conventional nitriding furnaces, and as a result, gas consumption was reduced by up to 80%.

• Journal of Dairy Science and Biotechnology
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• 제37권1호
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• pp.82-82
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• 2019

• Journal of Welding and Joining
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• 제35권1호
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• pp.49-54
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• 2017

In this study, the volumetric heat source in electron beam welding (EBW) is modeled through finite element method taking advantage of ABAQUS software package. Since this welding method is being applied in plates with different thicknesses and also considering that residual stresses reduce the strength of these weldments, the effect of thickness in the distribution and magnitude of residual stresses after welding is studied. Regarding the vast application of Inconel 706 super-alloy in aerospace industries, this material was selected in the current research. In order to validate the finite element model, the obtained results were compared to those of other researchers in this area, and good agreement was observed. The simulation results revealed that increase in the plate thickness leads to increase in the residual stresses. In addition heat treatment in the base metal (before welding) increases the residual stresses significantly.

• 한국정밀공학회지
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• 제22권11호
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• pp.51-58
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• 2005

Laser surface hardening technologies have been used to improve characteristics of wear and to enhance the fatigue resistance for automotive parts. The objective of this research work is to investigate the influence of the process parameters, such as power of laser and defocused spot position, on the characteristics of laser heat treatment for the case of SM45C medium carbon steel. CW Nd:YAG laser is selected as the heat source. The optical lens with the elliptical profile is designed to obtain a wide heat treatment area with a uniform hardness. From the results of the experiments, it has been shown that the maximum hardness is approximatly 780 Hv when the power and the travel speed of laser are 1,095 W and 0.6 m/min, respectively. In addition, the hardening width using the elliptical lens was three time larger than that using the defocusing of laser beam.

• 상하수도학회지
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• 제24권6호
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• pp.683-690
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• 2010

Renewable and unutilized energy (biogas power generation, wind power, solar, small hydro-power, sewage heat source, etc.) seems to be suitable to install for the sewage treatment facilities. There are 357 sewage treatment plants in 2007. 17 plants among these have been operating for self-supporting energy by using solar power, small hydro-power and biogas in 2008. Newly built sewage treatment plant of 96,000 $m^3$/day for a newtown is expected to get up to energy consumption of 10 GWh/yr. If solar energy, small hydro-power and biogas-equipments were applied to the new treatment plant, self-supporting energy of the new sewage treatment plant will get up to 56.1%. As a results, about 2,379ton $CO_2$/yr $CO_2$ emission reduction can be expected by using renewable energy. These efforts for self-supporting energy will lead sewage treatment plant to new energy recycle center.