• BMB Reports
• /
• v.39 no.6
• /
• pp.749-758
• /
• 2006

The cytosolic members of the HSP70 family of proteins play key roles in the molecular chaperone machinery of the cell. In the study we cloned and sequenced the full-length cDNA of Delia antiqua HSP70 gene, which is 2461 bp long and encodes 643 a.a. with a calculated molecular mass of 70,787 Da. We investigated gene copies of cytosolic HSP70 members of 4 insect species with complete genome available, and found that they are quite variable with species. In order to characterize this protein we carried out an alignment and a phylogenetic analysis with 41 complete protein sequences from insects. The analysis divided the cytosolic members of the family into two classes, HSP70 and HSC70, distinguishable on the basis of 15 residues. HSP70 class members were slightly shorter in length and smaller in molecular mass relative to the HSC70 class members, and the conservative and functional regions in these sequences were documented. Mainly, we investigated the expression of Delia antiqua HSP70 gene, in response to diapauses and thermal stresses. Both summer and winter diapauses elevated HSP70 transcript levels. Cold-stress led to increased HSP70 expression levels in summer- and winter-diapausing pupae, but heat-stress elevated the levels only in the winter-diapausing pupae. In all cases, the expression levels, after being elevated, gradually decreased with time. HSP70 expression was low in non-diapausing pupae but was up-regulated following cold- and heat-stresses. Heat-stress gradually increased the mRNA level with time whereas cold-stress gradually decreased levels after an initial increase.

• Nuclear Engineering and Technology
• /
• v.42 no.6
• /
• pp.620-635
• /
• 2010

In this paper we describe current activities on the project Multi-Scale Modeling and Analysis of convective boiling (MSMA), conducted jointly by the Paul Scherrer Institute (PSI) and the Swiss Nuclear Utilities (Swissnuclear). The long-term aim of the MSMA project is to formulate improved closure laws for Computational Fluid Dynamics (CFD) simulations for prediction of convective boiling and eventually of the Critical Heat Flux (CHF). As boiling is controlled by the competition of numerous phenomena at various length and time scales, a multi-scale approach is employed to tackle the problem at different scales. In the MSMA project, the scales on which we focus range from the CFD scale (macro-scale), bubble size scale (meso-scale), liquid micro-layer and triple interline scale (micro-scale), and molecular scale (nano-scale). The current focus of the project is on micro- and meso-scales modeling. The numerical framework comprises a highly efficient, parallel DNS solver, the PSI-BOIL code. The code has incorporated an Immersed Boundary Method (IBM) to tackle complex geometries. For simulation of meso-scales (bubbles), we use the Constrained Interpolation Profile method: Conservative Semi-Lagrangian $2^{nd}$ order (CIP-CSL2). The phase change is described either by applying conventional jump conditions at the interface, or by using the Phase Field (PF) approach. In this work, we present selected results for flows in complex geometry using the IBM, selected bubbly flow simulations using the CIP-CSL2 method and results for phase change using the PF approach. In the subsequent stage of the project, the importance of effects of nano-scale processes on the global boiling heat transfer will be evaluated. To validate the models, more experimental information will be needed in the future, so it is expected that the MSMA project will become the seed for a long-term, combined theoretical and experimental program.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1999.04a
• /
• pp.209-214
• /
• 1999

Strength provisions in Korea Concrete Institute code are more conservative that those in ACI code by increasing load factors and decreasing capacity reduction factors. Cement content of mix design in construction field is usually higher than the modified for standard deviation because of rigorous inspection. Higher cement content increases not only strengths but also heat of hydration, shrinkage and brittleness which are not beneficial. To reduce and optimize the cement content in current mix design of Korean Highway Corporation, properties of fresh and hardened concrete for 16 different mix proportions have been investigated. It is found that the chemical admixture and cement of current mix proportions for highway construction are somewhat higher than the optimum amount. Therefore, the optimum mix design for 16 different purposes has been proposed.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.19 no.3
• /
• pp.731-740
• /
• 1995

The objective of this paper is to develop defect assessment technology for integrity evaluation of CANDU pressure tubes. In fracture mechanics analysis, three-dimensional and two-dimensional (line-spring model) finite element analyses were performed to obtain the stress intensity factor for axial and circumferential surface cracks. In leak before break (LBB) analysis, heat transfer analyses for through-wall cracks were performed by considering the cooling effect and the LBB application time was computed. It was shown that the analytical results obtained in this study provide less-conservative but accurate solution for defect assessment of CANDU pressure tubes.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.24 no.11
• /
• pp.2812-2822
• /
• 2000

Several methods have been developed to predict the rupture time of the boiler tubes in thermal power plant. However, existing life prediction methods give very conservative value at operating stress of power plant and rupture strain cannot be well estimated. Therefore, in this study, rupture time and strain prediction method accounting for creep, corrosion and heat transfer is newly proposed and compared with the current research results. The creep damage evolves by continuous cavity nucleation and constrained cavity growth. The corrosion damage evolves by steam side and fire side corrosion. The results showed good correlation between the theoretically predicted rupture time and the current research results. And rupture strain may be well estimated by using the proposed method.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.5 no.4
• /
• pp.284-292
• /
• 1981

The flow of fluid with low prandtl number in the entrance region of a circular pipe has been considered, where the wall temperature is maintained to be constant. A finite difference method is used for the integral form of the governing equations in order that they satisfy the conservative properties of the numerical solutions. It is confirmed that the hydrodynamic entrance length and be divided into growing boundary layer region and fully viscous region, which is compared with existing results obtained by using boundary layer approximations. By assuning the developing velocity profile in the entrance region, the thermal entrance length is estimated and the local Nusselt number is obtained at various locations along the axial dirction.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.31 no.4
• /
• pp.335-341
• /
• 2005

This study discusses the effects of infrared thermography in dentistry. Infrared thermography allows the quantification of infrared rays and provides useful data for dental care and research. It has been used for the diagnosis of temporomandibular dysfunction (TMD) and proposed as an objective measure of myofascial pain. It is closely related to temperature and produces beneficial objective data. A study of the heat emissions from facial structures has the potential to benefit dental diagnosis of TMD, postoperative inferior alveolar damage, and endodontic and conservative treatments. In this paper, we assessed infrared thermography in dentistry and consider future developments of infrared thermography.

• Journal of Korean Dental Science
• /
• v.15 no.2
• /
• pp.112-120
• /
• 2022

In this article, the contemporary root canal treatment procedure using nickel-titanium (NiTi) instruments was reviewed to understand the correlations between the properties of files and safety of the clinical usage. Literatures were reviewed according to the process of clinical procedure of the root canal preparation, mainly for shaping during orifice flaring, glide-path preparation, and main canal instrumentation. Considering the reasons for NiTi file fracture, clinically implacable issues and ideas were discussed to reduce the fracture risk and increase clinical efficiency of the NiTi file systems. Various kinds of NiTi file systems have their own characteristics and properties given from their geometries and heat treatments and so on. Proper selection and careful usage of the NiTi file systems may reduce the risk of file fracture and increase the efficiency of NiTi file systems. Understanding of the clinical implications from the mechanical properties and characteristics of the engine driven NiTi instruments may decrease the risk of NiTi file fractures and increase the success rate in root canal treatment.

• Restorative Dentistry and Endodontics
• /
• v.27 no.6
• /
• pp.587-599
• /
• 2002

Extracellular single unit recordings were made from the ventral posteromedial thalamic (VPM) nociceptive neurons to determine mechanoreceptive field (RF) and response properties. A total of 44 VPM thalamic nociceptive neurons were isolated from rats anesthetized with urethane-chloralose. Based on responses to various mechanical stimuli including touch, pressure and pinch applied to the RF, 32 of 44 neurons were classified as nociceptive specific (NS) neuron. The other 12 neurons, classified as wide dynamic range (WDR), showed a graded response to increasingly intense stimuli, with a maximum discharge to noxious pinch. The VPM nociceptive neurons showed various spontaneous activity ranged from 0-6 Hz. They were located throughout the VPM, and had an contralateral RF including mainly intraoral (and perioral) regions. The RF size was relatively small, and very few neurons had a receptive field involving 3 trigeminal divisions. The NS neurons activated only by pressure and pinch stimuli had high mechanical thresholds compared to WDR neurons activated also by touch stimuli. The VPM nociceptive neurons were tested with suprathershold graded mechanical stimuli. Most of 21 NS and 8 WDR neurons showed a progressive increase in number of spikes as mechanical stimulus intensity was increased. In some neurons, the responses reached a peak before the highest intensity was given. Application of 5 mM $CoCl_2{\;}(10{\;}{\mu}\ell)$ solution to the trigeminal subnucleus caudalis did not produce any significant changes in the spontaneous activity, RF size, mechanical threshold, and response to suprathreshold mechanical stimuli of 9 VPM nociceptive neurons tested. 17 of 33 VPM nociceptive neurons responded to noxious heat as well as noxious mechanical stimuli applied to their RF. Application of the mustard oil, a small-fiber excitant and inflammatory irritant, to the right maxillary first molar tooth pulp induced an immediate but short-lasting neuronal discharges upto approximately 4 min in 16 of 42 VPM nociceptive neurons. These results suggest that VPM thalamic nucleus may contribute to the sensory discriminative aspect of orofacial nociception.

• International Journal of Oral Biology
• /
• v.35 no.2
• /
• pp.43-49
• /
• 2010

Enterococcus faecalis, a gram-positive bacterium, has been implicated in endodontic infections, particularly in chronic apical periodontitis. Proinflammatory cytokines, including tumor necrosis factor-$\alpha$ (TNF-$\alpha$), are involved in the pathogenesis of these apical lesions. E. faecalis has been reported to stimulate macrophages to produce TNF-$\alpha$. The present study investigated the mechanisms involved in TNF-$\alpha$ production by a murine macrophage cell line, RAW 264.7 in response to exposure to E. faecalis. Both live and heat-killed E. faecalis induced high levels of gene expression and protein release of TNF-$\alpha$. Treatment of RAW 264.7 cells with cytochalasin D, an inhibitor of endocytosis, prevented the mRNA up-regulation of TNF-$\alpha$ by E. faecalis. In addition, antioxidant treatment reduced TNF-$\alpha$ production to baseline levels. Inhibition of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein (MAP) kinase also significantly attenuated E. faecalis-induced TNF-$\alpha$ expression by RAW 264.7 cells. Furthermore, activation of NF-${\kappa}B$ and AP-1 in RAW 264.7 cells was also stimulated by E. faecalis. These results suggest that the phagocytic uptake of bacteria is necessary for the induction of TNF-$\alpha$ in E. faecalis-stimulated macrophages, and that the underlying intracellular signaling pathways involve reactive oxygen species, ERK, p38 MAP kinase, NF-${\kappa}B$, and AP-1.