• Title/Summary/Keyword: heat and irradiation

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Protein Kinase C-$\beta$ Is Induced In Ionizing Irradiation Induced Pigmentation

  • Nelly Rubeiz;Park, Dee-Young;Barbara A. Gilchrest
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.209-212
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    • 2002
  • Cutaneous hyperpigmentation is a well-known consequence of both acute and chronic X-irradiation, although the molecular mechanisms involved are not well understood. Recently, protein kinase C-$\beta$ (PKC-$\beta$) was shown to activate tyrosinase, a key and the rate-limiting enzyme in melanogenesis [1]. In this study, we have investigated its role in mediating ionizing radiation-induced pigmentation by exposing cultured human melanocytes to X-irradiation. Increased tyrosinase activity after the 4 Gys exposure was observed within 48 hrs and total melanin content doubled after 7 days. Interestingly, tyrosinase mRNA level was not affected by X-irradiation. However, there was a 2-3 fold increase in PKC-$\beta$ mRNA after 48 hours of irradiation, coinciding with the increase in tyrosinase activity. This induction was not due to non-specific heat generated during the irradiation because when melanocytes were incubated at 4$0^{\circ}C$, there was no induction of PKC-$\beta$ mRNA. Taken together, these data suggest that X-irradiation induces cutaneous hyperpigmentation, at least in part, by up-regulating the level of PKC-$\beta$.

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Effect of Laser Processing Patterns on the Bonding Interface Quality during Laser Sintering of Magnesium Alloys with Zirconia (마그네슘 합금 표면의 지르코니아 분말 레이저 소결과정에서 조사 패턴이 접합 계면 품질에 미치는 영향)

  • Yoon, Sangwoo;Kim, Joohan
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.20 no.2
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    • pp.51-57
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    • 2021
  • The quality of the ceramic sintered coating on a metal surface through laser surface treatment is affected by the laser irradiation pattern. Depending on the laser irradiation pattern, the amount of residual stress and heat applied or accumulated on the surface increases or decreases, affecting the thickness attained in the ceramic sintering area. When the heat energy accumulated in the sintering area is high, the ceramic and the metal alloy melt and sufficiently mix to form a homogeneous and thick bonding interface. In this study, the thermal energy accumulation in the region sintered with zirconia was controlled using four types of laser processing patterns. The thickness of the diffusion region is analyzed by laser-induced breakdown spectroscopy of Mg-ZrO2 generated by laser sintering zirconia powder on the magnesium alloy surface. On the basis of the analysis of the Mg and Zr present in the sintered region through LIBS, the effect of the irradiation pattern on the sintering quality is confirmed by comparing and analyzing the heat and mass transfer tendency of the diffusion layer and the degree of diffusion according to the irradiation pattern. The derived diffusion coefficients differed by up to 9.8 times for each laser scanning pattern.

Effects of Heat Treatment on the Antigenicity of Gamma-Irradiated Egg White Albumin (감마선 조사된 난백 알부민의 항원성에 미치는 가열의 영향)

  • 이주운;육홍선;조경환;김미리;김천제
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.848-853
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    • 2001
  • This research was conducted to study the effects on antigenicities (allergenicity) and structural changes of gamma-irradiated hen’s egg albumin (ovalbumin, OVA) by heating. Three groups of OVA solution (2.0 mg/mL) were prepared; 1) heat treatment; 2) irradiation after heating; 3) heating after irradiation. Samples were isothermally heated and/or irradiated at the absorption dose of 10 kGy. Competitive indirect ELISA was individually formatted with egg-allergic patients IgE (P-IgE), and mouse murine monoclonal IgG (M-IgG) and rabbit polyclonal IgG (R-IgG) for evaluating bindinhg abilities of antibodies to OVA in the sample solutions. Binding abilities of antibodies to thermally denatured OVA were changed : R-IgG to the sample treated with above 6$0^{\circ}C$, M-IgG to that above 7$0^{\circ}C$, and P-IgE to that above 8$0^{\circ}C$, respectively. P-IgE did not well recognize OVA heated at 8$0^{\circ}C$ and the above. However, binding abilities of M-IgG and R-IgG highly in creased. Significant differences of binding abilities were not observed in all samples with the combination of heat treatment and irradiation, regardless the order of the treatment. Turbidity of samples in creased both by heating and by irradiation, and the increase by irradiation was much higher than by heating. These results showed that allergenicity of OVA reduced by gamma irradiation was not affected by heating.

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Effect of Microwave Irradiation on Crystallinity and Pasting Viscosity of Corn Starches Different in Amylose Content

  • Lee, Su-Jin;Sandhu, Kawaljit Singh;Lim, Seung-Taik
    • Food Science and Biotechnology
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    • v.16 no.5
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    • pp.832-835
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    • 2007
  • Moisture content of normal, waxy, and high amylose com starches was adjusted to 10-35%, and irradiated in a microwave oven. The effect of microwave irradiation on the crystalline structure of starch was measured by using a differential scanning calorimetry (DSC), and X-ray diffractometry. Pasting viscosity profile was also determined by using a rapid viscoanalyzer (RVA). For all the 3 types of starches tested, the rate of temperature increase by the microwave irradiation was faster and more rapidly reached the maximum temperature of the pressure bomb ($120^{\circ}C$) when the moisture content was higher. X-ray diffraction and DSC data revealed that the microwave irradiated starch underwent partial disruption of crystalline structure. RVA studies showed that the irradiation caused significant reductions in maximal viscosity and breakdown, whereas pasting temperature was increased. Overall trends revealed that the microwave irradiation on the starch containing limited moisture content (less than 35%) provided the effects similar to the heat moisture treatment. These effects became more significant when the moisture content was higher. Compared to waxy com starch, normal, and high amylose com starches appeared to be more susceptible to the microwave irradiation.

Effect of Heat-Treated Temperature on Surface Crystal Structure and Catalytic Activity of ACF/ZnO Composite under Ultraviolet Irradiation and Ultrasonication

  • Zhang, Kan;Oh, Won-Chun
    • Journal of the Korean Ceramic Society
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    • v.47 no.2
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    • pp.136-141
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    • 2010
  • ACF/ZnO photocatalyst was synthesized by a sol-gel method using activated carbon fiber (ACF) and Zn $(NO_3)_2$ as precursors. Samples were characterized by Brunauer-Emmett-Teller measurements (BET), scanning electron microscope (SEM), X-ray diffraction (XRD), and energy dispersive X-ray (EDX). The XRD results showed that ACF/ZnO composites only included a hexagonal phase by heat-treated temperature at $400^{\circ}C$, $500^{\circ}C$, $600^{\circ}C$, and $700^{\circ}C$. The SEM analysis revealed that the ACF/ZnO composites did not exhibit any morphological changes of the catalyst surface according to the different heat-treated temperatures. The photocatalytic activity of the samples was tested for degradation of methylene blue (MB) solutions under ultraviolet (UV) light and ultrasonication respectively. The results showed that the photocatalytic activity of ACF/ZnO composites heat-treated at $500^{\circ}C$ was higher than other samples, which is ascribed to the fine distribution of ZnO particles on the surface of the ACF. In addition, an ultrasound of low power (50 W) was used as an irradiation source to successfully induce ACF/ZnO composites to perform sonocatalytic degradation of MB. Results indicated that the sonocatalytic method in the presence of ACF/ZnO composites is an advisable choice for the treatments of organic dyes.

Direct Microwave Sintering of Poorly Coupled Ceramics in Electrochemical Devices

  • Amiri, Taghi;Etsell, Thomas H.;Sarkar, Partha
    • Journal of Electrochemical Science and Technology
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    • v.13 no.3
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    • pp.390-397
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    • 2022
  • The use of microwaves as the energy source for synthesis and sintering of ceramics offers substantial advantages compared to conventional gas-fired and electric resistance furnaces. Benefits include much shorter processing times and reaching the sintering temperature more quickly, resulting in superior final product quality. Most oxide ceramics poorly interact with microwave irradiation at low temperatures; thus, a more complex setup including a susceptor is needed, which makes the whole process very complicated. This investigation pursued a new approach, which enabled us to use microwave irradiation directly in poorly coupled oxides. In many solid-state electrochemical devices, the support is either metal or can be reduced to metal. Metal powders in the support can act as an internal susceptor and heat the entire cell. Then sufficient interaction of microwave irradiation and ceramic material can occur as the sample temperature increases. This microwave heating and exothermic reaction of oxidation of the support can sinter the ceramic very efficiently without any external susceptor. In this study, yttria stabilized zirconia (YSZ) and a Ni-YSZ cermet support were used as an example. The cermet was used as the support, and a YSZ electrolyte was coated and sintered directly using microwave irradiation without the use of any susceptor. The results were compared to a similar cell prepared using a conventional electric furnace. The leakage test and full cell power measurement results revealed a fully leak-free electrolyte. Scanning electron microscopy and density measurements show that microwave sintered samples have lower open porosity in the electrode support than conventional heat treatment. This technique offers an efficient way to directly use microwave irradiation to sinter thin film ceramics without a susceptor.

Enhanced Electrical and Optical Properties of IWO Thin Films by Post-deposition Electron Beam Irradiation (증착 후 전자빔 조사에 따른 IWO 박막의 전기적, 광학적 특성 개선 효과)

  • Jae-Wook Choi;Sung-Bo Heo;Yeon-Hak Lee;Daeil Kim
    • Journal of the Korean Society for Heat Treatment
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    • v.36 no.5
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    • pp.298-302
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    • 2023
  • Transparent and conducting tungsten (W) doped indium oxide (IWO) thin films were deposited on the glass substrate by using RF magnetron sputtering and then electron irradiation was conducted to investigate the effect of electron irradiation on the optical and electrical properties of the films. The electron irradiated films showed three x-ray diffraction peaks of the In2O3 (222), (431) and (046) planes and the full width at half maximum values are decreased as increased electron irradiation energy. In the atomic force microscope analysis, the surface roughness of as deposited films was 1.70 nm, while the films electron irradiated at 700 eV, show a lower roughness of 1.28 nm. In this study, the figure of merit (FOM) of as deposited films is 2.07 × 10-3-1, while the films electron irradiated at 700 eV show the higher FOM value of 5.53 × 10-3-1. Thus, it is concluded that the post-deposition electron beam irradiation is the one of effective methods to enhance optical and electrical performance of IWO thin films.

Influence of Electron Irradiation on the Structural Electrical and Optical Properties of ITO/Ti bi-layered Films (전자빔 조사에 따른 In2O3/Ti 적층박막의 전기적, 광학적 특성 변화)

  • Moon, Hyun-Joo;Jeon, Jae-Hyun;Song, Young-Hwan;Oh, Jung-Hyun;Gong, Tae-Kyung;Choi, Dong-Hyuk;Son, Dong-Il;Kim, Daeil
    • Journal of the Korean Society for Heat Treatment
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    • v.28 no.6
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    • pp.310-314
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    • 2015
  • We have considered the influence of electron irradiation on the optical and electrical properties of $In_2O_3/Ti$ bi-layered films prepared with RF and DC magnetron sputtering. The $In_2O_3/Ti$ thin films irradiated at 600 eV shows the lowest resistivity of $6.9{\times}10^{-4}{\Omega}cm$. The optical transmittance in a visible wave length region also influenced with the electron irradiation energy. The film that electron irradiated at 600 eV shows 82.9% of optical transmittance in this study. By comparison of figure of merit, it is concluded that the opto-electrical performance of $In_2O_3/Ti$ bi-layered film is improved with electron irradiation.

Effects of gamma irradiation on the functional and structural characteristics of soy protein isolates (방사선 조사가 분리대두단백의 기능적, 구조적 특성에 미치는 영향)

  • 오경남;이숙영
    • Korean journal of food and cookery science
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    • v.20 no.3
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    • pp.256-264
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    • 2004
  • The effects of irradiation on the functional and structural characteristics of soy protein isolates were studied. Soymilk was irradiated at 1, 5, and l0kGy, after which soy protein isolates were prepared. The functional properties of soy protein isolates were examined including solubility, emulsion capacity and stability, foam capacity and stability, structural properties as represented by SDS-PAGE pattern, and secondary and tertiary structures. The solubility and emulsion capacity were increased by radiation treatment at 1kGy however the values were adversely affected again as dosage was increased above 5kGy. As irradiation dosage increased, an increase of foaming capacity at 1kGy and a decreasing turnover afterwards were also noted in foaming capacity, although the differences were not statistically significant. The SDS-PAGE pattern showed fragmentation and aggregation of protein molecules as affected by irradiation in proportion to the dosage increase. The results of CD and fluorescence spectroscopy revealed increased aperiodic structure contents with the dosage increase. It was assumed that irradiation dosagefrom 5 to l0kGy could initiate minimal denaturation of protein in various foods compared to general heat treatment.

Technology of the End Cap Laser Welding for Irradiation Fuel Rods (조사연료봉 봉단마개의 레이저용접기술)

  • 김수성;이정원;고진현;이영호
    • Journal of Welding and Joining
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    • v.21 no.6
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    • pp.20-25
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    • 2003
  • Various welding methods such as Gas Tungsten Arc Welding(GTAW), magnetic force electrical resistance welding and Laser Beam Welding(LBW) are now available for end cap closure of nuclear fuel rods. Even though the resistance and GTA welding processes are widely used in manufacturing commercial fuel rods, they can not be recommended for the remote seal welding of fuel rods in the hot cell Facility due to the complexity of the electrode alignment, the difficulty in replacing parts in a remote manner and the large heat input for the thin sheath. Therefore, the Nd:YAG laser system using optical fiber transmission was selected for the end cap welding of irradiation fuel rods in the hot cell. The remote laser welding apparatus in the hot cell Facility was developed using a pulsed Nd:YAG laser of 500 watt average power with an optical fiber transmission. The weldment quality such as microstructure and mechanical strength was satisfactory. The optimum conditions of laser welding for encapsulating irradiation fuel rods in the hot cell were obtained.