• Archives of Plastic Surgery
• /
• 제33권1호
• /
• pp.21-30
• /
• 2006

Alginate, a polymer of guluronic and mannuronic acid, is used as a scaffolding material in biomedical applications. The research was to produce highly-purified alginate from seaweeds and to evaluate the efficacy of alginate as dermal substrate. Our alginate purification method showed a production rate as high as 25%. The purified alginate contained little polyphenol contents and endotoxin, proteins. For study of wound healing, full thickness skin defects were made on the dorsal area of the animal models. And then alginate, fibroblast-growth-factor mixed alginate, alginate-collagen complex, vaseline gauze as control were applied on the wound, respectively, and were evaluated grossly and histopathologically. For biocompatibility test, alginate and alginate-collagen complex discs were implanted on the back of Sprague-Dawly rats. Four weeks after implantation, the animals were examined immunologically against alginate and collagen. Alginate and FGF-mixed alginate, alginate-collagen complex group showed statistically higher percentage of wound contraction and wound healing than control group(p<0.05). Alginate-collagen complex group and FGF-mixed alginate group showed statistically higher percentage of wound healing than alginate group. The experiment of biocompatibility and immunologic reaction against impanted alginate or collagen needs more investigation. Highly-purified alginate from seaweeds by our purification method, showed the effect of wound healing, and addition of FGF or collagen increases the alginate's wound healing effect. It shows the possibility of alginate as a dermal substrate.

• 한국해양바이오학회지
• /
• 제12권1호
• /
• pp.66-74
• /
• 2020

Marine algae contain a variety of substances, such as mycosporine-like amino acids, which can defend against UV irradiation. Among them, Porphyra-334 derived from Porphyra yezoensis is attracting attention as a novel active ingredient for anti-aging cosmetics because of its excellent anti-oxidative, anti-inflammatory, and wound-healing properties through promoting skin cell migration. In this study, a process using direct current (DC) for increasing the yield of large-scale purification of Porphyra-334 was developed. When DC was applied to obtain Porphyra-334 efficiently, the purification time was shortened by approximately 1/4 compared with the process wherein DC was not applied; moreover, the yield of purification was improved.

• 한국환경생태학회지
• /
• 제30권2호
• /
• pp.277-286
• /
• 2016

본 연구는 숲에서 치유를 경험한 사람들의 체험수기 115편을 질적 분석하여, 숲 치유로 나타나는 핵심 심리현상과 치유의 과정을 추적하기 위하여 수행되었다. 숲 치유 과정에서 경험하는 핵심 심리현상은 <행복감> <편안함> <깨달음>의 순이었으며, 숲 치유의 과정은 <정서적 변화>를 먼저 경험한 후에 <인지적 변화>와 <행동의 변화>로 이끌어지는 경향을 나타내었다. 숲에서의 치유단계는 시간의 흐름에 따라 <자극단계> <수용단계> <정화단계> <통찰단계> <채움단계> <변화단계>의 6단계 과정을 거쳤으며, 이러한 과정은 <자연과의 교감> <자신과의 교감> <세상과의 소통>이라는 기제를 통해 치유가 진전되는 것으로 확인되었다. 기존의 연구는 주로 집단프로그램에 의한 비교 연구와 체험후의 일시적 상태의 측정으로 결과를 도출하여 효과의 지속성이나 심리적 변화 과정을 검증할 수 없는 한계가 존재하였다. 본 연구는 기존의 양적 연구의 한계를 보완하여 개인적 심리현상과 치유과정 등을 통합적으로 이해할 수 있는 방법을 제안하였으며, 연구 결과가 산림치유의 메커니즘을 밝히고 산림치유 프로그램 개발이나 실행에 기초자료로 활용될 수 있을 것으로 기대한다.

• 센서학회지
• /
• 제32권4호
• /
• pp.213-218
• /
• 2023

Triboelectric nanogenerators (TENGs) have emerged as a highly promising energy harvesting technology capable of harnessing mechanical energy from various environmental vibrations. Their versatility in material selection and efficient conversion of mechanical energy into electric energy make them particularly attractive. TENGs can serve as a valuable technology for self-powered sensor operation in preparation for the IoT era. Additionally, they demonstrate potential for diverse applications, including energy sources for implanted medical devices (IMDs), neural therapy, and wound healing. In this review, we summarize the potential use of this universally applicable triboelectric energy harvesting technology in the disinfection and blocking of pathogens. By integrating triboelectric energy harvesting technology into human clothing, masks, and other accessories, we propose the possibility of blocking pathogens, along with technologies for removing airborne or waterborne infectious agents. Through this, we suggest that triboelectric energy harvesting technology could be an efficient alternative to existing pathogen removal technologies in the future.

• 산업식품공학
• /
• 제22권4호
• /
• pp.353-357
• /
• 2018

Previous studies have suggested that rice bran oil (RBO), an edible oil from the byproducts of rice milling, has anti-inflammatory effects in inflammation inducing macrophages, known as M1 subsets. Yet the effects of RBO on the counterpart M2 subsets, the "healing" macrophages, were poorly investigated to date. In this regard, recent studies on the molecular/cellular anti-inflammatory mechanisms of dietary components have demonstrated that mitochondrial respiration contributes to macrophage functioning. Therefore, the current study examined whether RBO regulates cytokine secretion by modulating mitochondrial metabolism in wound healing M2 subsets. Palm oil (PO), enriched with medium-chain fatty acids, served as a positive control. C57BL/6 mice were fed a diet containing either corn oil (CO), PO or RBO for 4 weeks, followed by purification of bone marrow-derived macrophages (BMDM) from their tibias and femurs. Cells were further polarized to M2-BMDM, and the expression of M2 marker (CD206) on cellular surfaces were not affected by dietary intervention. In addition, the secretion of anti-inflammatory cytokine (IL-10) in the culture supernatant was not affected by dietary lipids. Oxygen consumption rate, the indicator of mitochondrial respiration in M2-BMDM was not regulated by RBO intervention and PO treatment. Taken together, this study imply that RBO did not intervene both the regulation of inflammatory responses and mitochondrial respiration in M2 macrophages.

• 한국산학기술학회논문지
• /
• 제16권1호
• /
• pp.78-85
• /
• 2015

광대는 주로 우스꽝스러운 모습과 바보 같은 행동으로 사람들을 웃게 만드는 역할을 한다. "리어왕"에 등장하는 바보 광대 또한 특유의 익살과 해학으로 우리에게 즐거움을 주고 있다. 하지만, 그는 진정한 바보가 아니다. 그는 현명함을 지니고 있고 사람들의 마음을 꿰뚫어보는 직관력을 가지고 있으며, 언제나 현실을 직시한다. 그는 미래를 예측하고, 옳지 않은 것에 대해 비판하며, 또한 상처 받은 마음을 치유하는 역할을 한다. 그는 항상 리어왕의 옆을 지키며, 리어왕이 그 자신의 어리석음을 깨닫도록 도움을 준다. 모든 고통과 두려움에서 벗어난 리어왕은 광대의 도움으로 자신의 감정이 순화되고 치유되는 과정을 겪게 된다. 이러한 면에서 "리어왕"에 등장하는 바보광대는 리어왕의 현명한 조력자이자 치유자인 것이다.

• 대한방사성의약품학회지
• /
• 제7권2호
• /
• pp.113-118
• /
• 2021

Chitosan is a polysaccharide derived from chitin by deacetylation. Chitosan is non-toxic, biodegradable, and biocompatible, so that it can be used in wide variety of medical applications such as wound healing and antimicrobial biomaterials. It also used as dermal fillers due to its ability to inject with liquid formulations. For investigation on in vivo distribution of these chitosans, Bolton-Hunter-conjugated chitosan (Chitosan-BH) was synthesized by the reaction between the primary amino group of chitosan and N-hydroxysuccinimide ester group of Bolton-Hunter reagent. Then Chitosan-BH was radiolabeled with ¹²⁵I (Chitosan-BH-¹²⁵I) using a Chloramine-T method. The effects of each radiolabeling step on the radiolabeling yield of the final product were tested. The results showed that purification step had significant effects on the radiolabeling yield of the final product. Finally, SPECT/CT images were obtained to evaluate in vivo uptake of the radiolabeled chitosan (Chitosan-BH-¹²⁵I) in several organs. The highest uptake was found in the site of injection at 21 days post-injection. The results of this study suggest that chitosan is expected to be useful for biomaterials of dermal fillers.

• 한국자연치유학회지
• /
• 제11권1호
• /
• pp.18-30
• /
• 2022

배경: 제주도 화산섬 지하자원인 현무암 "숲" 지하 공기(숨골)의 효과를 검증하기 위하여 '지하 공기정화 층을 이용한 공기정화시스템(APS)'을 개발한 후에 정화 공기 체험장을 설치하였다. 이 정화 공기를 채취하여 분석하고 인체에 유용성에 관한 탐색연구가 필요하다. 목적:현무암 "숲" 지하 공기(숨골)를 APS로 채취하여 성분을 분석하고, 이 공기를 체험한 후에 인체에 미치는 영향을 탐색하는 것이었다. 방법: 제주도의 파파빌레 지역의 4개의 지점에 APS 장치를 설치하였다. APS에서 배출하는 지하 공기를 채집하여 자원화 성분을 분석하였다. 또한 공기 밀폐 연구 체험장을 지상에 설치하였으며, 이 체험장의 음이온 농도는 5,000 ions/m³정도이었고, 체험자들이 60~120분 정도 머무르게 한 후에 혈관 상태를 조사하였다. 결과: 현무암 '숲' 지하 공기를 채취하여 분석한 결과는 O₂ 농도는 21.18%로 대기의 평균 산소 농도 20.94%보다 높았다. 폼알데하이드는 검출되지 아니하였고, CO₂농도는 419 ppm으로 실내공기보다 낮았다. 미세먼지(PM2.5) 농도는 24 ㎍/ms 이하였으며, 음이온도 5.000 ions/m³ 이상 검출되었다. 체험한 사람들의 혈관 건강 지수 개선되었고, 평균 맥박의 증가 및 스트레스 해소 등의 효과가 높게 나타났다. 결론: 현무암 "숲" 지하공기(숨골)를 분석하여 규명한 유용한 성분은 자연치유 등에 활용할 가치가 높았고, 체험한 결과에서는 맥박의 증진, 혈관과 스트레스 개선에 효과를 나타냈다. 이러한 조건은 제주지역의 현무암 숲이 자연치유와 웰니스 산업으로 확장하기 위한 새로운 지역이라고 높게 평가할 수 있다.

• 인간식물환경학회지
• /
• 제23권6호
• /
• pp.603-614
• /
• 2020

Background and objective: This study was conducted to understand urban residents' perception of indoor plants in order to assist in the preparation of guidelines for growing plants indoors. Methods: Questionnaires were distributed to urban residents currently growing plants or with experience of growing plants. The data of 247 respondents were subjected to analysis to assess their level of interest and ability with regard to growing plants, and their recognition of the selection and function of plants. Results: Respondents showed high interest in growing plants at a level of 6.77, but their ability was moderate. This suggests that information regarding plant maintenance should be provided to urban residents. They recognized the function of plants for improving the environment as important, and expected that indoor plants would improve their mood and visual experience. Satisfaction with growing plants was high from an emotional perspective. They recognized that the function of air purification function was important even if the actual performance or effect was not great. This indicates that the function of air purification should be emphasized more. As the result of the IPA, plants should be selected in consideration of morphological characteristics such as leaf, flower and fruit, and continuously managed to maintain their characteristics. Since the performance of pots was less satisfactory compared to their importance, they should be selected so that they harmonize better with plants and are of the appropriate size to fit the space. The type of plants is an important factor in plant selection in order to perform an environmental function, and the flower color and pot size are important in terms of aesthetics and healing. Conclusion: When horticulture information on indoor plants is provided to urban residents, first, it should be provided to satisfy the functions of the plants required by urban residents and in order to promote and maintain the proper growth of plants. Second, various contents that utilize components should be developed to achieve plant function so that resident can determine the function of plants and select the type, quantity, and shape of plants to achieve the desired function.

• 농업과학연구
• /
• 제48권4호
• /
• pp.1003-1013
• /
• 2021

Thioredoxin (TRX) protein is an antioxidant responsible for reducing other proteins by exchanging cysteine thiol-disulfide and is also known for its anti-allergic and anti-aging properties. On the other hand, epidermal growth factor (EGF) is an important material used in the cosmetics industry and an essential protein necessary for dermal wound healing facilitated by the proliferation and migration of keratinocytes. EGF also assists in the formation of granulation tissues and stimulates the motility of fibroblasts. Hence, genetically modified soybeans were developed to overexpress these industrially important proteins for mass production. A single-dose oral-toxicity-based study was conducted to evaluate the potential toxic effects of TRX and EGF proteins, as safety assessments are necessary for the commercial use of seed-specific protein-expressing transgenic soybeans. To achieve this rationale, TRX and EGF proteins were mass purified from recombinant E. coli. The single-dose oral-toxicity tests of the TRX and EGF proteins were carried out in six-week old male and female Institute of Cancer Research (ICR) mice. The initial evaluation of the single-dose TRF and EGF treatments was based on monitoring the toxicity signatures and mortality rates among the mice, and the resultant mortality rates did not show any specific clinical symptoms related to the proteins. Furthermore, no significant differences were observed in the weights between the treatment and control groups of male and female ICR mice. After 14 days of treatment, no differences were observed in the autopsy reports between the various treatment and control groups. These results suggest that the minimum lethal dose of TRX and EGF proteins is higher than the allowed 2,000 mg·kg^-1 limit.