• 한국동물학회지
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• 제17권2호
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• pp.51-56
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• 1974

生後 24시간 이내의 어린 Armenian hamster와 Chinese hamster의 體內에 adenovirus type 12를 注入할 경우, 전자에서는 27일, 후자에서는 30$\\sim$45일이 지난후 腫瘍모양을 한 작은 細胞傀가 생김을 보았다. 이들 細胞傀는 다른 個體에 移植이 가능하며, 上皮細胞와 같은 모양을 하고 있다. 本實驗에서 얻어진 57개의 細胞傀 중에서 6개만이 細胞培養 되었으며 14$\\sim$20회 정도 繼代培養을 계속하는 동안에 染色體의 변동은 없이 細胞의 모양이 纖維狀으로 변하였다. 이들 細胞가 上皮細胞 모양을 할 때는 腫瘍性이며, T-抗體도 발견되었는데, 纖維狀으로 변하게 되면 腫瘍性을 나타내지 않으며, T-抗體도 찾아 볼 수 없다. 다른 2개의 細胞傀를 上皮細胞 모양일 때 SV40 바이러스로 처리하면 바로 纖維狀細胞로 변한다. 이들 細胞에는 adenovirus에 의한 T-抗體는 없으나, SV40의 T-抗體가 존재하며, 다른 hamster에 移植할 경우 皮腫 모양의 細胞傀를 이룬다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5779-5785
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• 2015

The present study was designed to investigate cholangiocarcinoma (CCA) antibodies in hamster serum. Hamster CCA cell lines were processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A candidate biomarker was confirmed by immunoprecipitation and western blot, and was further analyzed using ELISA and sera from normal control hamsters, hamsters with opisthorchiasis and hamsters with various stages of CCA, as well as from CCA patients and healthy individuals. One candidate marker was identified as $HSP90{\alpha}$, as indicated by a high level of anti-$HSP90{\alpha}$ in hamster CCA sera. It was found that the levels of anti-$HSP90{\alpha}$ were specifically elevated in the sera of hamsters with CCA compared with other groups and progressively increased with the clinical stage. At the cut-off point of 0.4850 on the receiver operating characteristic curve, anti-$HSP90{\alpha}$ could discriminate CCA from healthy control groups with a sensitivity of 76.2%, specificity of 71.4% and total accuracy 75.5%. In the present study, we have shown that anti-$HSP90{\alpha}$ may be a potential useful serum biomarker to discriminate CCA cases from healthy persons.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.951-957
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• 2013

Our aim was to investigate the chemopreventive potential of saffron in DMBA-induced hamster buccal pouch carcinogenesis. Assessment was by monitoring the percentage of tumor bearing hamsters, tumor size as well as the status of detoxification agents, lipid peroxidation and antioxidants. Oral squamous cell carcinomas were induced in the buccal pouch of Syrian golden hamsters by painting them with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. We observed 100% oral tumor formation with severe histopathological abnormalities in all the hamsters treated with DMBA alone, activities of phase I and phase II detoxification enzymes, lipid peroxidation and antioxidants being significantly altered. Though oral administration of saffron completely prevented the formation of tumors, we noticed severe hyperplasia and dysplasia in hamsters treated with DMBA, suggesting that tumors might eventually develop. Oral administration of saffron return detoxification enzymes, lipid peroxidation and antioxidants to normal ranges. The chemopreventive potential of saffron thus is likely due to antioxidant properties and modulating effects on detoxification in favour of the excretion of carcinogenic metabolites during DMBA-induced hamster buccal pouch carcinogenesis.

• Preventive Nutrition and Food Science
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• 제7권2호
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• pp.133-138
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• 2002

To examine the cholesterol-towering effects of extruded products made with cassava starch (CS) and blends of cassava starch with either resistant starch (CS-RS) or oat fiber (CS-OF) hamsters were fed with diets containing a high-cholesterol (2%) and high-fat (17%) diet for 20 days. Hamsters fed with a diet containing no cholesterol were used as a control. Total cholesterol (TC) levels in the CS-RS and CS-OF groups were significantly (p>0.05) lower compared to the CS group by 11.5% and 8.5%, respectively. Also, the diets containing fibers decreased the value of low-density lipoproteins plus very low-density lipoproteins fraction by 32.4% (CS-RS diet) and 51.7% (CS-OF), respectively, as compared to the CS diet. Total lipid values were significantly (p<0.05) lower in hamsters fed the CS-RS diet (916 mg/dL) and CS-OF diet (964 mg/dL) as compared to those fed the CS diet (1661 mg/dL). The results obtained in this study suggest that extruded products containing cassava starch blended with either resistant starch or oat fleer, could prevent health problems associated with high levels of cholesterol and hypertriglyceridemia induced by a high cholesterol and fat diet.

• 대한수의학회지
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• 제34권1호
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• pp.127-134
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• 1994

As a series of studies to investigate the effect of immunosuppression on Ascaris suum infection in undefinitive hosts, a delicate relationship between host and parasite, in the present studies golden hamsters were alloted to experiment 1(normal undefinitive host group) and experiment 2(immunosuppressive group treated with prednisolone acetate) and inoculated with a single dose of 1,500 embryonated Ascaris suum eggs. The recovery rates, sizes and features of the larvae and immunological responses in the hamsters were chronologically monitored according to somatic migration. In both experiments, the larvae failed to develop into the adults, but the more and larger larvae were observed for a longer period from experiment 2 in comparison with experiment 1. The numbers of the mast cells in the small intestinal mucosa and mesenteric lymph nodes, of the goblet cells in the small intestinal mucosa and of T-cells in the mesenteric lymph nodes, spleens and cardiac blood from experiment 2 were fewer than those from the experiment 1. In general, increasing of these cells followed by expulsion of the worms in the both groups. Profound leukopenia due to lymphopenia was found through trial period in experiment 2. Considering the experimental results, development or expulsion mechanism of somatic migrant larvae may be related to lymphopenia and temporary increasing tendency of the mast cells, the goblet cells and T-cells. In addition, patent infection of A suum in the hamsters was not obviously observed in spite of immunosuppression by prednisolone acetate.

• 대한수의학회지
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• 제36권1호
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• pp.169-179
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• 1996

This study was carried out to examine the role of small cells and oval cells in cholangiocarcinogenesis in the hamsters infected with Clonorchis(C) sinensis. Forty two female Syrian golden hamsters were divided into two groups. Group I was for the induction of the cholangiocarcinoma, which was infected orally with C sinensis and given dimethylnitrosamine(15ppm) in drinking water for 4 weeks. Group II was served as control. More than 5 heads of hamsters in each group were sacrificed at 4, 7, 11 and 15 weeks after the beginning of the experiment. The livers were examined histopathologically, electron microscopically and immunohistochemically. The results obtained were as follows; 1. Cholangiocarcinomas were occurred in 1 of 6 animals at 11 weeks and in 4 of 6 animals at 15weeks after the beginning of the experiment. 2. Small cells and oval cells were proliferated around the portal triads from 4 weeks and peaked at 11 weeks, and slightly decreased after then. 3. The strong positive reaction to the $\alpha$-fetoprotein was shown in many of small cells and oval cells. But ductlike oval cells, which were arranged rosette form, showed week positive reaction to the $\alpha$-fetoprotein. 4. Most of small cells and oval cells showed negative reaction to the cytokeratin. But weak positive reaction in ductlike oval cells, and moderate positive reaction in cholangiocarcinoma cells were observed. These results suggested that cholangiocarcinoma induced by infection of C sinensis was believed to originate from the proliferated small cells around the portal triads which would be able to differentiate to the oval cells, ductlike oval cells, and cholangiocarcinoma cells gradually.

• 한국발생생물학회지:발생과생식
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• 제6권1호
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• pp.45-54
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• 2002

골든 햄스터의 생식활동은 광주기에 의해 조절된다. 그들의 생식능력은 여름에 왕성하고 겨울에는 퇴화한다. 송과선에서 분비되는 멜라토닌은 계절적 번식동물에서 생식활동을 중재한다. 멜라토닌 수용체가 최근에 사람을 포함하는 몇몇 동물에서 확인되었지만 골든 햄스터의 생식능력과 관련하여 알려진 바가 많지 않다. 역전사 PCR 방법을 사용하여 멜라토닌 수용체의 일부 유전자를 동정하였다(309 염기). 멜라토닌 수용체의 핵산 서열과 추론된 아미노산 서열을 보고된 다른 동물들과 비교하였다. 멜라토닌 수용체는 시상하부, 뇌하수체, 혈액, 지라에서 명백히 탐지되었다. 광주기가 정소 무게 및 생식 호르몬에 현저한 영향을 주었지만 멜라토닌 수용체의 발현에는 영향을 뚜렷하게 보이지 않았다. 이러한 결과는 생식을 조절하는 멜라토닌은 그 수용체의 수보다 수용체에 결합하는 친화도가 더 중요함을 시사한다.

• 한국발생생물학회지:발생과생식
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• 제24권4호
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• pp.263-275
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• 2020

The proper administration of melatonin has well been documented to induce testicular regression in seasonal breeding animals. The subcutaneous injections of melatonin in the afternoon, not in the morning, consistently occurred testicular involution in the male Syrian (golden) hamsters whose reproductive activity is regulated by the photoperiod. But the effects of daily melatonin via gavage have not been estimated. Golden hamsters housed in long photoperiod (LP) were divided into 5 groups: the control animals housed in LP or in short photoperiod (SP) and animals treated daily with low (15 ㎍), middle (150 ㎍), and high dosages (1,500 ㎍) of pure melatonin by using gavage in the evening for 8 weeks. As results, LP control animals had large testes and SP controls displayed small and entirely regressed testes. The animals treated with various dosages of melatonin showed collectively degenerating effects on the weights of testes, epididymides, and seminal vesicles in the middle and high dosage groups, with the individual differences as well. The high dosages induced testicular regression in more proportion than the middle dosages did. The low dosage had large testes like the LP control animals. The small and inactive testes shown in some animals of both middle and high groups presented the complete regression as those of the animals maintained in SP. These results strongly suggest that the administrations of melatonin lead to testicular involution in the male golden hamsters when it is administered through gavage.

• Journal of Photoscience
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• 제9권2호
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• pp.252-254
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• 2002

Djungarian hamsters show distinct seasonal rhythms in several physiological parameters. One of them is daily torpor that occurs in winter with decreased body temperature (about 1O-20$^{\circ}$C) during daytime. Daily torpor is induced by short-day photoperiod, food restriction and castration. But the mechanism to induce daily torpor has not been clarified. In the present study, we tried to clarify the process of daily torpor induction in detail. Adult male hamsters were kept in long photoperiod and high temperature (LP-HT) before the experiment and, thereafter, the animals were transferred to short photoperiod and low temperature (SP-LT), and they were kept in this condition for about six months. The daily rhythms of locomotor activity and body temperature were recorded every three-minutes by using the Minimitter telemetry system. Locomotor activity and body temperature showed very closely synchronized rhythms. All animals under LP-HT showed daily rhythms with higher locomotor activity and body temperature in nighttime than in daytime. Under SP-LT, there were two types of animals with and without showing daily torpor. Thus, they have individual differences in the response to SP -LT.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5753-5757
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• 2012

Apoptosis, also known as cell suicide or programmed cell death, removes unwanted and genetically damaged cells from the body. Evasion of apoptosis is one of the major characteristic features of rapidly proliferating tumor cells. Chemopreventive agents inhibit or suppress tumor formation through apoptotic induction in target tissues. The aim of the present study was to investigate the pro-apoptotic potential of lupeol during 7,12-dimethylbenz(a) anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Topical application of 0.5% DMBA three times a week for 14 weeks in the buccal pouches of golden Syrian hamsters resulted in oral squamous cell carcinoma. The expression pattern of apoptotic markers was analyzed using immunohistochemistry (p53, Bcl-2, Bax) and ELISA reader (caspase 3 and 9). In the present study, 100% tumor formation with defects in apoptotic markerexpression pattern was noticed in hamsters treated with DMBA alone. Oral administration of lupeol at a dose of 50mg/kg bw completely prevented the formation oral tumors as well as decreased the expression p53 and Bcl-2, while increasing the expression of Bax and the activities of caspase 3 and 9. The present study thus indicated that lupeol might inhibit DMBA-induced oral tumor formation through its pro-apoptotic potential in golden Syrian hamsters.